PCR-cloning of goldfish and tilapia metallothionein complementary DNAs.

Abstract:

:Metallothionein (MT) is believed to be a sensitive and effective "biomarker" for monitoring metal contamination in fish. Comparison of amino acid sequences between flounder MT and trout MT revealed that there is a conserved region at their N-terminals. Using oligonucleotides derived from this conserved region, reverse transcription- polymerase chain reaction (RT-PCR) was performed to obtain MT complementary DNAs (cDNAs) from goldfish, Carassius auratus, and tilapia, Tilapia mossambica. These cDNA probes would be useful in developing sensitive PCR-based methods to detect MT gene expression for monitoring metal pollution in local waters.

authors

Chan KM

doi

10.1006/bbrc.1994.2674

subject

Has Abstract

pub_date

1994-11-30 00:00:00

pages

368-74

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(84)72674-X

journal_volume

205

pub_type

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