当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > HSP27 phosphorylation protects against endothelial barrier dysfunction under burn serum challenge.

HSP27 phosphorylation protects against endothelial barrier dysfunction under burn serum challenge.

Abstract：

:F-actin rearrangement is an early event in burn-induced endothelial barrier dysfunction. HSP27, a target of p38 MAPK/MK2 pathway, plays an important role in actin dynamics through phosphorylation. The question of whether HSP27 participates in burn-related endothelial barrier dysfunction has not been identified yet. Here, we showed that burn serum induced a temporal appearance of central F-actin stress fibers followed by a formation of irregular dense peripheral F-actin in pulmonary endothelial monolayer, concomitant with a transient increase of HSP27 phosphorylation that conflicted with the persistent activation of p38 MAPK/MK2 unexpectedly. The appearance of F-actin stress fibers and transient increase of HSP27 phosphorylation occurred prior to the burn serum-induced endothelial hyperpermeability. Overexpressing phospho-mimicking HSP27 (HSP27(Asp)) reversed the burn serum-induced peripheral F-actin rearrangement with the augmentation of central F-actin stress fibers, and more importantly, attenuated the burn serum-induced endothelial hyperpermeability; such effects were not observed by HSP27(Ala), a non-phosphorylated mutant of HSP27. HSP27(Asp) overexpression also rendered the monolayer more resistant to barrier disruption caused by Cytochalasin D, a chemical reagent that depolymerizes F-actin specifically. Further study showed that phosphatases and sumoylation-inhibited MK2 activity contributed to the blunting of HSP27 phosphorylation during the burn serum-induced endothelial hyperpermeability. Our study identifies HSP27 phosphorylation as a protective response against burn serum-induced endothelial barrier dysfunction, and suggests that targeting HSP27 wound be a promising therapeutic strategy in ameliorating burn-induced lung edema and shock development.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Sun HB,Ren X,Liu J,Guo XW,Jiang XP,Zhang DX,Huang YS,Zhang JP

doi

10.1016/j.bbrc.2015.04.152

subject

Has Abstract

pub_date

2015-07-31 00:00:00

pages

377-83

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(15)00997-3

journal_volume

463

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Decreased expression of glutaredoxin 1 is required for transforming growth factor-beta1-mediated epithelial-mesenchymal transition of EpRas mammary epithelial cells.

    abstract：:Transforming growth factor-beta (TGF-beta) is a cytokine important in inducing epithelial-mesenchymal transition (EMT), a crucial morphological event in a wide range of physiological and pathological cellular processes. In this study, we demonstrate that TGF-beta1 induces the EMT phenotype through decreasing the expre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.12.009

    authors： Lee EK,Jeon WK,Chae MY,Hong HY,Lee YS,Kim JH,Kwon JY,Kim BC,Park SH

    更新日期：2010-01-01 00:00:00

  • TLR4 activates NF-κB in human ovarian granulosa tumor cells.

    abstract：:Previous studies have demonstrated expression of Toll-like receptors (TLRs) in the surface epithelium of normal ovaries (OSE) and in epithelial ovarian tumors. Most notably, OSE-derived cancers express TLR4, which activates the nuclear factor-kappa B (NF-κB) signaling cascade as a mediator of inflammatory response. Cu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.05.063

    authors： Woods DC,White YA,Dau C,Johnson AL

    更新日期：2011-06-17 00:00:00

  • Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system.

    abstract：:In the case of a new viral disease outbreak, an immediate development of virus detection kits and vaccines is required. For COVID-19, we established a rapid production procedure for SARS-CoV-2 spike protein (S protein) by using the baculovirus-silkworm expression system. The baculovirus vector-derived S proteins were ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.06.020

    authors： Fujita R,Hino M,Ebihara T,Nagasato T,Masuda A,Lee JM,Fujii T,Mon H,Kakino K,Nagai R,Tanaka M,Tonooka Y,Moriyama T,Kusakabe T

    更新日期：2020-08-20 00:00:00

  • Regulation of the solubilized bovine cerebral cortex muscarinic receptor by GTP and Na+.

    abstract：:The muscarinic acetylcholine receptor was solubilized, in a sensitive form for GTP and Na+, from bovine cerebral cortex using a zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane sulfonate. The solubilized muscarinic receptor displayed characteristics as follows: (1) high affinity to nanomolar co...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91709-6

    authors： Kuno T,Shirakawa O,Tanaka C

    更新日期：1983-05-16 00:00:00

  • Identification of two novel insulin receptor mutations, Asp59Gly and Leu62Pro, in type A syndrome of extreme insulin resistance.

    abstract：:To elucidate genetic determinants of insulin resistance, we investigated insulin receptor (IR) and insulin receptor substrate-1 (IRS-1) genes, in vitro IR function and in vivo insulin sensitivity in a family with Type A syndrome. Two missense IR mutations (Asp59Gly and Leu62Pro) found in the proband, resulted in reduc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6695

    authors： Rouard M,Macari F,Bouix O,Lautier C,Brun JF,Lefebvre P,Renard E,Bringer J,Jaffiol C,Grigorescu F

    更新日期：1997-05-29 00:00:00

  • Elafin is a potent inhibitor of proteinase 3.

    abstract：:Elafin, a human skin derived inhibitor of human leukocyte elastase, was tested for inhibitory activity against proteinase 3, an elastin degrading proteinase of neutrophils. The inhibitory activity of elafin was compared with antileukoprotease and eglin C. Elafin proved to be a potent inhibitor of elastin-FITC degradat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90476-n

    authors： Wiedow O,Lüademann J,Utecht B

    更新日期：1991-01-15 00:00:00

  • Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact.

    abstract：:Epimorphin, a mesenchymal cell surface-associated molecule, is detected on hepatic stellate cells (HSCs) in the liver. Here, we show the involvement of epimorphin in differentiation of rat hepatic stem-like cells (HSLCs) through contact with HSCs. HSLCs, isolated from adult rats, cultured in stellate cell-conditioned ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.225

    authors： Miura K,Nagai H,Ueno Y,Goto T,Mikami K,Nakane K,Yoneyama K,Watanabe D,Terada K,Sugiyama T,Imai K,Senoo H,Watanabe S

    更新日期：2003-11-14 00:00:00

  • Cisplatin induced alterations in oriented fibers of DNA studied by atomic force microscopy.

    abstract：:Oriented fibers of DNA, prepared by the wet spin method, were imaged by atomic force microscopy. It was found that an oriented fiber's substructure is an array of assemblages of DNA. For native DNA, the assemblages exhibit a characteristic width of 76 nm, a thickness of 20 nm, and appear to carry a right handed twist....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80131-7

    authors： Rampino NJ

    更新日期：1992-01-15 00:00:00

  • Radiation-inducible hSNK gene is transcriptionally regulated by p53 binding homology element in human thyroid cells.

    abstract：:We identified a species relevant to polo-like kinase family, a human homologue of mouse serum-inducible kinase, hSNK gene, whose mRNA expression was rapidly increased in cultured human thyroid cells after X-ray irradiation. The cDNA cloning and genomic analysis of the hSNK gene showed the presence of 14 exons spanning...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5993

    authors： Shimizu-Yoshida Y,Sugiyama K,Rogounovitch T,Ohtsuru A,Namba H,Saenko V,Yamashita S

    更新日期：2001-11-30 00:00:00

  • Rescue of ligand binding of a mutant IGF-I receptor by complementation.

    abstract：:The IGF-I receptor binds IGF-I with complex kinetics characterized by a curvilinear Scatchard plot, suggesting receptor heterogeneity and apparent negative cooperativity. To explore the molecular mechanisms underlying these properties, we have characterized the binding of a hybrid receptor formed from a wild-type rece...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.122

    authors： Chakravarty A,Hinrichsen J,Whittaker L,Whittaker J

    更新日期：2005-05-27 00:00:00

  • Suppressed hepatocyte proliferation via a ROS-HNE-P21 pathway is associated with nicotine- and cotinine-enhanced alcoholic fatty liver in mice.

    abstract：:CYP2A5 is a major enzyme responsible for nicotine and cotinine metabolism in mice. Nicotine and cotinine enhance alcoholic fatty liver in wild type (WT) mice but not in CYP2A5 knockout (KO) mice, and reactive oxygen species (ROS) generated during the CYP2A5-mediated metabolism contributes to the enhancing effect. In c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.021

    authors： Chen X,Wang K,Cederbaum AI,Lu Y

    更新日期：2019-04-23 00:00:00

  • Differential expression of components of the microRNA machinery during mouse organogenesis.

    abstract：:MicroRNA (miRNA)-mediated gene silencing has recently emerged as a major mechanism of gene expression regulation during development in a variety of species. Little is known, however, about the presence of components of miRNA machinery in mammalian organogenesis. In this study, we report that members of the Argonaute (...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.05.206

    authors： Lü J,Qian J,Chen F,Tang X,Li C,Cardoso WV

    更新日期：2005-08-26 00:00:00

  • The homeodomain of Tinman mediates homo- and heterodimerization of NK proteins.

    abstract：:Cardiac development requires the action of transcription factors, which control the specification and differentiation of cardiac cell types. One of these factors, encoded by the homeobox gene tinman (tin), is essential for the specification of all cardiac cells in Drosophila. An increasing number of examples show that...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.06.090

    authors： Zaffran S,Frasch M

    更新日期：2005-08-26 00:00:00

  • Specific expression of Neu2 type B in mouse thymus and the existence of a membrane-bound form in COS cells.

    abstract：:Neu2 mRNA from the mouse thymus, as we have reported [K. Kotani, A. Kuroiwa, T. Saito, Y. Matsuda, T. Koda, S. Kijimoto-Ochiai, Cloning, chromosomal mapping, and characteristic 5'-UTR sequence of murine cytosolic sialidase, Biochem. Biophys. Res. Commun. 286 (2001) 250-258], has a novel sequence at the 5' terminus tha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.07.100

    authors： Koda T,Kijimoto-Ochiai S,Uemura S,Inokuchi J

    更新日期：2009-10-02 00:00:00

  • Functional NifD-K fusion protein in Azotobacter vinelandii is a homodimeric complex equivalent to the native heterotetrameric MoFe protein.

    abstract：:The MoFe protein of the complex metalloenzyme nitrogenase folds as a heterotetramer containing two copies each of the homologous alpha and beta subunits, encoded by the nifD and the nifK genes respectively. Recently, the functional expression of a fusion NifD-K protein of nitrogenase was demonstrated in Azotobacter vi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.09.105

    authors： Lahiri S,Pulakat L,Gavini N

    更新日期：2005-11-18 00:00:00

  • Direct induction of hepatocyte-like cells from immortalized human bone marrow mesenchymal stem cells by overexpression of HNF4α.

    abstract：:Hepatocytes from human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are expected to be a useful source for cell transplantation. However, relatively low efficiency and repeatability of hepatic differentiation of human BM-MSCs remains an obstacle for clinical translation. Hepatocyte nuclear factor 4 alpha (HNF...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.08.026

    authors： Hu X,Xie P,Li W,Li Z,Shan H

    更新日期：2016-09-16 00:00:00

  • Determining the 3D structure of human ASC2 protein involved in apoptosis and inflammation.

    abstract：:ASC2 structure has been well defined by 1141 NOE experimental restraints. The model consists of five alpha helices. alpha-Helices are connected by short random structure loops. The sole exception is the loop connecting helices 2 and 3, which has a 20-residue length. Folding generally agrees with the folding of recentl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.087

    authors： Espejo F,Patarroyo ME

    更新日期：2006-02-17 00:00:00

  • CTRP3 plays an important role in the development of collagen-induced arthritis in mice.

    abstract：:Rheumatoid arthritis (RA) is an autoimmune inflammatory disease exhibited most commonly in joints. We found that the expression of C1qtnf3, which encodes C1q/TNF-related protein 3 (CTRP3), was highly increased in two mouse RA models with different etiology. To elucidate the pathogenic roles of CTRP3 in the development...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.040

    authors： Murayama MA,Kakuta S,Maruhashi T,Shimizu K,Seno A,Kubo S,Sato N,Saijo S,Hattori M,Iwakura Y

    更新日期：2014-01-03 00:00:00

  • Ang II induces capillary formation from endothelial cells via the AT1R-dependent inositol requiring enzyme 1 pathway.

    abstract：:Previous studies have demonstrated an important interaction between angiotension II type 1 receptor (AT1R) and angiotension II (Ang II) -induced capillary formation from endothelial cells and vascular endothelial growth factor (VEGF). However, the underlying mechanism remains elusive. Recent studies revealed that the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.03.113

    authors： Wang X,Bai YP,Hong D,Gao HC,Li LF,Li CC,Zhu LP,Sun Q,Zhang GG

    更新日期：2013-05-10 00:00:00

  • Alkaline low spin form of sulfite reductase hemeprotein subunit.

    abstract：:The reversible reduction and reoxidation of Escherichia coli sulfite reductase hemeprotein subunit at pH 9.9 produces high and low spin ferric species, the latter with properties distinct from any alkaline low spin yet reported. With virtually no effect on the 298 degrees K optical spectrum, chloride drastically reduc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91429-v

    authors： Young LJ,Siegel LM

    更新日期：1990-05-31 00:00:00

  • NMR-based metabolite profiling of human milk: A pilot study of methods for investigating compositional changes during lactation.

    abstract：:Low-molecular-weight metabolites in human milk are gaining increasing interest in studies of infant nutrition. In the present study, the milk metabolome from a single mother was explored at different stages of lactation. Metabolites were extracted from sample aliquots using either methanol/water (MeOH/H2O) extraction ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.11.114

    authors： Wu J,Domellöf M,Zivkovic AM,Larsson G,Öhman A,Nording ML

    更新日期：2016-01-15 00:00:00

  • MARs mission update.

    abstract：:Genes are organized as chromatin domains and positioned in the nucleus through regions of nuclear matrix association, termed MARs. Although well-studied, the mechanisms regulating expression of the beta-globin locus remain an enigma. Here, we show that certain MAR sequences of the beta-globin locus are conserved and r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7939

    authors： Walter WR,Singh GB,Krawetz SA

    更新日期：1998-01-14 00:00:00

  • Identification and gene structure of a novel human PLZF-related transcription factor gene, TZFP.

    abstract：:A novel cDNA clone was identified through yeast two-hybrid experiments. Following cross-examination between the cDNA clones, EST clones, and the cosmid clone, we could digitally assemble a new zinc finger transcription factor gene. This predicted gene has a cDNA size of about 1960 bp and is translated into a 487-amino...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1594

    authors： Lin W,Lai CH,Tang CJ,Huang CJ,Tang TK

    更新日期：1999-11-02 00:00:00

  • Transcriptome analysis of resistant and susceptible tobacco (Nicotiana tabacum) in response to root-knot nematode Meloidogyne incognita infection.

    abstract：:The root-knot nematode (RKN) Meloidogyne incognita reproduces on the roots of tobacco (Nicotiana tabacum), damaging crops, reducing crop yield, and causing economic losses annually. The development of resistant genotypes is an alternative strategy to effectively control these losses. However, the molecular mechanism r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.11.167

    authors： Xing X,Li X,Zhang M,Wang Y,Liu B,Xi Q,Zhao K,Wu Y,Yang T

    更新日期：2017-01-22 00:00:00

  • Curcumin does not stimulate cAMP-mediated chloride transport in cystic fibrosis airway epithelial cells.

    abstract：:It has been suggested that curcumin and other sarcoplasmic/endoplasmic reticulum Ca(2+)-pump inhibitors could correct the defect in the most common mutation (DeltaF508) in cystic fibrosis (CF), and restore normal chloride transport. In the present study, the effect of curcumin was tested on baby hamster kidney (BHK) c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.146

    authors： Dragomir A,Björstad J,Hjelte L,Roomans GM

    更新日期：2004-09-17 00:00:00

  • P53 modulates hepatic insulin sensitivity through NF-κB and p38/ERK MAPK pathways.

    abstract：:Besides its well-established oncosuppressor activity, the role of p53 in regulating metabolic pathways has been recently identified. Nevertheless, the function of p53 with respect to insulin resistance appears highly controversial. To address this issue, we investigated the expression of p53 in experimental model of i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.12.085

    authors： Geng S,Zhu W,Wang S,Xie C,Li X,Wu J,Li Y,Chen Y,Wang X,Meng Y,Zhang Q,Chen J,Zhong C

    更新日期：2018-01-15 00:00:00

  • Cytosolic alkalinization alone is not sufficient for Ca2+ mobilization, phosphatidic acid formation, and protein phosphorylation in human platelets.

    abstract：:One of the earliest events following stimulation of human platelets with thrombin is a rise in the cytosolic pH, pHi, mediated by Na+/H+ exchange, and an increase in the cytosolic free Ca2+ concentration, [Ca2+]i. In the present study we investigated whether an increase in pHi alone, induced by the Na+/H+ ionophore mo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91343-0

    authors： Siffert W,Akkerman JW

    更新日期：1989-06-30 00:00:00

  • Structure of a ribosomal 5S RNA from a mushroom, Coprinus cinereus.

    abstract：:The nucleotide sequence of the 5S rRNA from a mushroom, Coprinus cinereus, was determined to be: (sequence in text). This sequence is consistent with current models for the secondary structure of 5S RNAs and indicates a very high degree of sequence conservation among the most highly evolved fungi. Sequence heterogenei...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)90393-5

    authors： Liu WY,Nazar RN

    更新日期：1983-10-14 00:00:00

  • Effects of hydroxystilbene derivatives on tyrosinase activity.

    abstract：:Synthesis of melanin starts from the conversion of L-tyrosine to 3,4-dihydroxyphenylalanine (L-dopa) and then the oxidation of L-dopa yields dopaquinone by tyrosinase. Therefore, tyrosinase inhibitors have been established as important constituents of depigmentation agents. Recently, polyhydroxystilbene compounds, whi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(03)01284-1

    authors： Ohguchi K,Tanaka T,Kido T,Baba K,Iinuma M,Matsumoto K,Akao Y,Nozawa Y

    更新日期：2003-08-08 00:00:00

  • Constitutive over-expression of VEGF results in reduced expression of Hand-1 during cardiac development in Xenopus.

    abstract：:During heart development, various signaling cascades are tightly regulated in a stage- and region-dependent manner. Vascular endothelial growth factor (VEGF) is one of the important molecules required for both vascular development and cardiac morphogenesis. VEGF receptors are present in the embryonic heart, so we focu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.05.140

    authors： Nagao K,Taniyama Y,Koibuchi N,Morishita R

    更新日期：2007-08-03 00:00:00