当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Specific expression of Neu2 type B in mouse thymus and the existence of a membrane-bound form in COS cells.

Specific expression of Neu2 type B in mouse thymus and the existence of a membrane-bound form in COS cells.

Abstract：

:Neu2 mRNA from the mouse thymus, as we have reported [K. Kotani, A. Kuroiwa, T. Saito, Y. Matsuda, T. Koda, S. Kijimoto-Ochiai, Cloning, chromosomal mapping, and characteristic 5'-UTR sequence of murine cytosolic sialidase, Biochem. Biophys. Res. Commun. 286 (2001) 250-258], has a novel sequence at the 5' terminus that shows the ability to encode 6 extra amino acids in the N-terminus than that of the muscle. In this paper, we analyzed the cDNA and EST database and found the five types of alternative splicing of Neu2 mRNA: A, B, C, D and N. We studied the expression of these types in the immune tissues and found that the thymus expressed only type B. We constructed 2 types of plasmid that encode long (B) or short (C) form of Neu2 protein, and transfected them into COS7 cells to study them under the same conditions. We found that 30-40% of the both forms of Neu2 activity was located in the crude membrane-fraction, and hydrolyzed ganglioside effectively, while both soluble fraction showed particular behavior with substrate specificity. Microscopic study by active staining with X-NANA showed that they located not only in the cytoplasm but also in areas surrounding the nucleus and in the peripheral ruffled spot.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Koda T,Kijimoto-Ochiai S,Uemura S,Inokuchi J

doi

10.1016/j.bbrc.2009.07.100

subject

Has Abstract

pub_date

2009-10-02 00:00:00

pages

729-35

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)01460-0

journal_volume

387

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Analysis of the potency of various low molecular weight chemical chaperones to prevent protein aggregation.

    abstract：:Newly translated proteins must undergo proper folding to ensure their function. To enter a low energy state, misfolded proteins form aggregates, which are associated with many degenerative diseases, such as Huntington's disease and chronic kidney disease (CKD). Recent studies have shown the use of low molecular weight...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.019

    authors： Upagupta C,Carlisle RE,Dickhout JG

    更新日期：2017-04-22 00:00:00

  • A computergraphic determination of the chemotactic peptide preferred conformation.

    abstract：:Replacement of leucine in the chemotactic peptide For-Met-Leu-Phe by the sterically constrained amino acids alpha-aminoisobutyric acid and aminocyclohexanecarboxylic acid affords compounds of equal or greater activity than the parent. NMR studies indicate that the parent compound is present as a beta-sheet in solution...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80287-0

    authors： Semus SF,Becker EL,Toniolo C,Freer RJ

    更新日期：1988-12-15 00:00:00

  • Ca2+-dependent interaction between calmodulin and CoDN3, an effector of Colletotrichum orbiculare.

    abstract：:Nuclear magnetic resonance (NMR) data directly indicated a Ca2+-dependent interaction between calmodulin (CaM) and CoDN3, a small effector of the plant pathogenic fungus Colletotrichum orbiculare, which is the causal agent of cucumber anthracnose. The overall conformation of CoDN3 is intrinsically disordered, and the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.05.007

    authors： Isozumi N,Inoue Y,Imamura T,Mori M,Takano Y,Ohki S

    更新日期：2019-06-30 00:00:00

  • Tumor promoter inhibition of cellular binding of somatostatin.

    abstract：:Tumor promoting phorbol esters inhibited the binding of 125I-[Tyr11] somatostatin to isolated acinar cells from guinea-pig pancreas. Maximal inhibition reached 69.7 +/- 5% at 1 microM TPA. Receptor affinity was decreased by 2.5-fold without change in binding capacity. The ability of TPA in inhibiting somatostatin bind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90124-x

    authors： Zeggari M,Susini C,Viguerie N,Esteve JP,Vaysse N,Ribet A

    更新日期：1985-04-30 00:00:00

  • Liposome-encapsulated clodronate specifically depletes spinal microglia and reduces initial neuropathic pain.

    abstract：:Liposome-encapsulated clodronate (LEC) is a specific depletor of macrophages. Our study characterized the LEC depletory effects, given intrathecally, on spinal microglia and assessed its effects on initiation and maintenance of neuropathic pain. Measured by using the MTT assay, LEC treatment specifically inhibited cel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.03.177

    authors： Wang YR,Mao XF,Wu HY,Wang YX

    更新日期：2018-05-15 00:00:00

  • RecA-ssDNA filaments supercoil in the presence of single-stranded DNA-binding protein.

    abstract：:Using atomic force microscopy (AFM), we find that RecA-single-stranded DNA (RecA-ssDNA) filaments, in the presence of single-stranded DNA-binding (SSB) protein, organize into left-handed bundles, which differ from the previously reported disordered aggregates formed when SSB is excluded from the reaction. In addition,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.014

    authors： Shi WX,Larson RG

    更新日期：2007-06-08 00:00:00

  • Endothelin-1 peptides and IL-5 synergistically increase the expression of IL-13 in eosinophils.

    abstract：:The 21-amino acid-length endothelin-1 (ET-1)(1-21) and its novel derivative, 31 amino acid-length ET-1(1-31), have proinflammatory properties and induce significant eosinophil migration mediated by an increase in the local levels of eotaxin and IL-5. We have analyzed by reverse transcription polymerase chain reaction ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.01.168

    authors： Cui P,Sharmin S,Okumura Y,Yamada H,Yano M,Mizuno D,Kido H

    更新日期：2004-03-19 00:00:00

  • Down regulation of c-myc, c-fos and erb-B during estrogen induced proliferation of the chick oviduct.

    abstract：:Oncogenes c-myc, H-ras, c-fos and erb-B were constitutively expressed in immature chick oviduct withdrawn from estrogen administration for 2.5 weeks after 10 d of primary estrogen stimulation. Following secondary estrogen stimulation of the withdrawn chicks, synthesis of egg white proteins is rapidly induced and remai...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90489-5

    authors： Cohrs RJ,Goswami BB,Sharma OK

    更新日期：1988-01-15 00:00:00

  • Ochratoxin A decreases the activity of phosphoenolpyruvate carboxykinase and its mRNA content in primary cultures of rat kidney proximal convoluted tubule cells.

    abstract：:The effect of ochratoxin A on the specific activities of phosphoenolpyruvate carboxykinase, gamma-glutamyl transpeptidase and phosphate-dependent glutaminase were investigated in primary cultures of rat kidney proximal convoluted tubule cells grown in a serum-free medium supplemented with growth factors. Addition of o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)90759-6

    authors： Thekkumkara TJ,Patel MS

    更新日期：1989-08-15 00:00:00

  • The N-terminal internal region of BLM is required for the formation of dots/rod-like structures which are associated with SUMO-1.

    abstract：:Bloom Syndrome (BS) is a human autosomal genetic disorder characterized by a predisposition to a variety of malignant tumors. The gene responsible for BS encodes a protein (BLM) consisting of 1417 amino acids with a nuclear localization signal in the C-terminal region, which is a member of the RecQ helicase family. We...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5387

    authors： Suzuki H,Seki M,Kobayashi T,Kawabe Yi,Kaneko H,Kondo N,Harata M,Mizuno S,Masuko T,Enomoto T

    更新日期：2001-08-17 00:00:00

  • A common peptide stretch among enzymes localized to the Golgi apparatus: structural similarity of Golgi-associated glycosyltransferases.

    abstract：:A common peptide motif has been discovered among a series of Golgi-localized glycosyltransferases. The peptide stretch, (Ser/Thr)-X-(Glu/Gln)-(Arg/Lys), always occurs near a hydrophobic domain close to the N-terminus of these enzymes which is believed to anchor them to the membrane lipid bilayer (Paulson and Colley, J...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92173-w

    authors： Bendiak B

    更新日期：1990-07-31 00:00:00

  • Rapamycin targeting mTOR and hedgehog signaling pathways blocks human rhabdomyosarcoma growth in xenograft murine model.

    abstract：:Rhabdomyosarcomas (RMS) represent the most common childhood soft-tissue sarcoma. Over the past few decades outcomes for low and intermediate risk RMS patients have slowly improved while patients with metastatic or relapsed RMS still face a grim prognosis. New chemotherapeutic agents or combinations of chemotherapies h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.05.001

    authors： Kaylani SZ,Xu J,Srivastava RK,Kopelovich L,Pressey JG,Athar M

    更新日期：2013-06-14 00:00:00

  • Effect of endothelin-1 on release of arginine-vasopressin from perifused rat hypothalamus.

    abstract：:Endothelin-1 (ET-1) is an endothelium-derived vasoconstrictor peptide with potent pressor activity. We studied the effect of ET-1 on release of arginine-vasopressin (AVP) from perifused rat hypothalamus. ET-1 (10(-10) to 10(-8) M) significantly stimulated AVP release. The ET-1-induced AVP release was completely blocke...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91124-8

    authors： Shichiri M,Hirata Y,Kanno K,Ohta K,Emori T,Marumo F

    更新日期：1989-09-29 00:00:00

  • Enhancement of tubulin polymerization by Cl(-)-induced blockade of intrinsic GTPase.

    abstract：:In growing neurite of neuronal cells, it is suggested that α/β-tubulin heterodimers assemble to form microtubule, and assembly of microtubule promotes neurite elongation. On the other hand, recent studies reveal importance of intracellular Cl(-) in regulation of various cellular functions such as cell cycle progressio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.07.072

    authors： Nakajima K,Niisato N,Marunaka Y

    更新日期：2012-08-24 00:00:00

  • Mechanism-based inhibition of cancer metastasis with (-)-epigallocatechin gallate.

    abstract：:Cell motility and cell stiffness are closely related to metastatic activity of cancer cells. (-)-Epigallocatechin gallate (EGCG) has been shown to inhibit spontaneous metastasis of melanoma cell line into the lungs of mice, so we studied the effects of EGCG on cell motility, cell stiffness, and expression of vimentin ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.10.094

    authors： Takahashi A,Watanabe T,Mondal A,Suzuki K,Kurusu-Kanno M,Li Z,Yamazaki T,Fujiki H,Suganuma M

    更新日期：2014-01-03 00:00:00

  • The transcription factor MEF2C mediates cardiomyocyte hypertrophy induced by IGF-1 signaling.

    abstract：:Myocyte enhancer factor 2C (MEF2C) plays an important role in cardiovascular development and is a key transcription factor for cardiac hypertrophy. Here, we describe MEF2C regulation by insulin-like growth factor-1 (IGF-1) and its role in IGF-1-induced cardiac hypertrophy. We found that IGF-1 addition to cultured rat ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.07.147

    authors： Muñoz JP,Collao A,Chiong M,Maldonado C,Adasme T,Carrasco L,Ocaranza P,Bravo R,Gonzalez L,Díaz-Araya G,Hidalgo C,Lavandero S

    更新日期：2009-10-09 00:00:00

  • Defective GATA-3 expression in Th2 LCR-deficient mice.

    abstract：:Th2 cell differentiation is critically influenced by transcription factor GATA-3 and by various cis-acting elements including enhancers, silencers and a locus control region (LCR) in the Th2 cytokine locus. Th2 LCR-deficient Th2 cells completely lost the expression of GATA-3 and the phosphorylation of STAT6. Histone 3...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.06.080

    authors： Hwang SS,Kim K,Lee GR

    更新日期：2011-07-15 00:00:00

  • Recombinant Cry3Aa has insecticidal activity against the Andean potato weevil, Premnotrypes vorax.

    abstract：:The Andean potato weevil, Premnotrypes vorax, an insect of the order Coleoptera, is a major cause of damage to potato crops in the Andean regions of South America. The insecticidal Cry proteins from Bacillus thuringiensis are useful biological pesticides, and some are toxic to Coleopteran insects. We overexpressed rec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3998

    authors： Gomez S,Mateus AC,Hernandez J,Zimmermann BH

    更新日期：2000-12-20 00:00:00

  • Amino-acid substitutions at the domain interface affect substrate and allosteric inhibitor binding in α-isopropylmalate synthase from Mycobacterium tuberculosis.

    abstract：:α-Isopropylmalate synthase (α-IPMS) is a multi-domain protein catalysing the condensation of α-ketoisovalerate (α-KIV) and acetyl coenzyme A (AcCoA) to form α-isopropylmalate. This reaction is the first committed step in the leucine biosynthetic pathway in bacteria and plants, and α-IPMS is allosterically regulated by...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.02.092

    authors： Huisman FH,Squire CJ,Parker EJ

    更新日期：2013-04-05 00:00:00

  • Sck interacts with KDR and Flt-1 via its SH2 domain.

    abstract：:Vascular endothelial growth factor (VEGF) is one of the major angiogenesis regulators. It binds to its tyrosine kinase receptors, KDR and Flt-1. However, little is known about their downstream signal transduction properties. We screened human brain cDNA library using the yeast two-hybrid system with the KDR cytoplasmi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9442

    authors： Igarashi K,Shigeta K,Isohara T,Yamano T,Uno I

    更新日期：1998-10-09 00:00:00

  • The retinoic acid and brain-derived neurotrophic factor differentiated SH-SY5Y cell line as a model for Alzheimer's disease-like tau phosphorylation.

    abstract：:The paired helical filaments of highly phosphorylated tau protein are the main components of neurofibrillary tangles (NFT) in Alzheimer's disease (AD). Protein kinases including glycogen synthase kinase 3 beta (GSK3beta), cyclin-dependent kinase 5 (Cdk5), and c-Jun N-terminal kinase (JNK) have been implicated in NFT f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.075

    authors： Jämsä A,Hasslund K,Cowburn RF,Bäckström A,Vasänge M

    更新日期：2004-07-02 00:00:00

  • Nanomolar concentrations of lysophosphatidylcholine recruit monocytes and induce pro-inflammatory cytokine production in macrophages.

    abstract：:Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.087

    authors： Olofsson KE,Andersson L,Nilsson J,Björkbacka H

    更新日期：2008-05-30 00:00:00

  • The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells.

    abstract：:Stem cells have been identified using the DNA-binding dye Hoechst 33342 and flow cytometry (FCM) in various tissues known as the side population (SP). The present study shows, for the first time, the presence of side population cells in human deciduous dental pulp cells (DPCs). Flow cytometric identification revealed ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.08.058

    authors： Wang J,Wei X,Ling J,Huang Y,Huo Y,Zhou Y

    更新日期：2010-09-24 00:00:00

  • Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria.

    abstract：:How rod-shaped bacteria form and maintain their shape is an important question in bacterial cell biology. Results from fluorescent light microscopy have led many to believe that the actin homolog MreB and a number of other proteins form long helical filaments along the inner membrane of the cell. Here we show using el...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.03.062

    authors： Swulius MT,Chen S,Jane Ding H,Li Z,Briegel A,Pilhofer M,Tocheva EI,Lybarger SR,Johnson TL,Sandkvist M,Jensen GJ

    更新日期：2011-04-22 00:00:00

  • WITHDRAWN: Erratum to "Tomato plants overexpressing CaKR1 enhanced tolerance to salt and oxidative stress" [Biochem. Biophys. Res. Commun. 363 (2007) 983-988].

    abstract：:This article has been withdrawn at the request of the author. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.11.049

    authors： Seong ES,Cho HS,Choi D,Joung YH,Lim CK,Hur JH,Wang MH

    更新日期：2007-11-21 00:00:00

  • Synthesis and processing of killer toxin from Ustilago maydis virus P4.

    abstract：:The synthesis of toxin protein from Ustilago maydis virus (UmV) strain P4 was studied in vitro and in vivo. The protein synthesized in vitro and in vivo has a molecular weight of approximately 30 kd whereas the native toxin has a molecular weight of about 12 kd. In the presence of protease inhibitors and glycosylation...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90379-2

    authors： Podila GK,Bozarth RF,Flurkey WH

    更新日期：1987-12-16 00:00:00

  • GLM, a novel luteolin derivative, attenuates inflammatory responses in dendritic cells: Therapeutic potential against ulcerative colitis.

    abstract：:GLM, a luteolin derivative, shows anti-melanogenic effect via regulation of various signal molecules; however, it is unclear whether it also exerts anti-inflammatory effect. This study investigated the mechanisms of the anti-inflammatory effect of GLM on activated dendritic cells (DCs) to elucidate its therapeutic pot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.012

    authors： Kim WS,Song HY,Han JM,Byun EB

    更新日期：2019-10-08 00:00:00

  • The tetrapeptide Arg-Leu-Tyr-Glu inhibits VEGF-induced angiogenesis.

    abstract：:Kringle 5, derived from plasminogen, is highly capable of inhibiting angiogenesis. Here, we have designed and synthesized 10 tetrapeptides, based on the amino acid properties of the core tetrapeptide Lys-Leu-Tyr-Asp (KLYD) originating from anti-angiogenic kringle 5 of human plasminogen. Of these, Arg-Leu-Tyr-Glu (RLYE...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.05.073

    authors： Baek YY,Lee DK,So JH,Kim CH,Jeoung D,Lee H,Choe J,Won MH,Ha KS,Kwon YG,Kim YM

    更新日期：2015-08-07 00:00:00

  • Ritonavir does not inhibit calpain in vitro.

    abstract：:Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and mu-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.11.161

    authors： Cuerrier D,Nie Z,Badley AD,Davies PL

    更新日期：2005-02-04 00:00:00

  • Evidence for the alpha-helicity of class II MHC molecular binding sites for the superantigen, staphylococcal enterotoxin A.

    abstract：:Circular dichroism (CD) spectra of class II MHC peptides revealed the alpha-helical conformation of superantigen-binding peptides I-A beta b(60-90), I-A beta b(65-85), and I-A alpha b(51-80), but not the nonbinding peptide I-A beta b(80-100). These CD spectra provide biophysical evidence for the alpha-helicity of clas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91833-c

    authors： Russell JK,Jarpe MA,Johnson HM

    更新日期：1992-02-14 00:00:00