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Upregulation of gene expression levels of ceramide metabolic enzymes after application of sphingomyelin-based liposomes to a three-dimensional cultured human epidermis model.

Abstract：

BACKGROUND/AIMS:We have previously reported that the application of sphingomyelin-based liposomes (SM-L) to a three-dimensional cultured skin model increase the content of ceramides NS, NP, AS and AP. However, the mechanism responsible for these increased ceramide levels was not identified. METHODS:SM-L and sphingomyelinase (SMase) were combined and incubated at 37 °C for 24 h. SM-L were also applied to three-dimensional cultured skin for 24 h and quantification of SMase and β-glucocerebrosidase (β-GCase) mRNA expression levels performed using real-time PCR. Additionally, three dimensional cultured skin was incubated with SM-L and the β-GCase inhibitor conduritol B epoxide (CBE) and the ceramide content determined by high performance thin layer chromatography. RESULTS:We observed generation of ceramide NS after reaction of SM-L and SMase. However, the other ceramide classes were not detected. Notably, SMase and β-GCase mRNA expression levels were significantly increased in cells of the skin model following application of SM-L. The levels of ceramides NS, NP, AS and AP were decreased by treatment with CBE. However, only ceramide NS was significantly increased by treatment with CBE and SM-L in combination. CONCLUSION:These findings indicate that application of SM-L to cultured skin upregulates the expression of SMase and β-GCase and increases ceramide content.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Itaya Y,Tokudome Y

doi

10.1016/j.bbrc.2016.03.061

subject

Has Abstract

pub_date

2016-04-22 00:00:00

pages

114-117

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)30373-4

journal_volume

473

pub_type

杂志文章

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