Abstract:
:Recombinant baculoviruses expressing full length and 3' truncated forms of c-DNA encoding the Drosophila melanogaster acetylcholinesterase (AChE) were constructed. Biochemical analyses showed that full length recombinant protein was enzymatically active and anchored to the cell membrane via a glycolipidic residue. DTT treatment dissociated the native form into monomers migrating as did the corresponding form of AChE extracted from drosophila heads. Finally, DFP labelling demonstrated that the specific proteolytic cleavage leading to the formation of 55 and 16 kDa subunits occurred in Sf9 cells. In contrast with the full-length enzyme, C-terminal-truncated forms were highly secreted, confirming the prominent role of the C-terminal hydrophobic peptide for the addition of the glycolipidic residue. Accumulation of inactive precursor was observed when recombinant proteins were overproduced using an improved baculovirus, suggesting a saturation of insect cell machineries.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Chaabihi H,Fournier D,Fedon Y,Bossy JP,Ravallec M,Devauchelle G,Cérutti Mdoi
10.1006/bbrc.1994.2243subject
Has Abstractpub_date
1994-08-30 00:00:00pages
734-42issue
1eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)72243-1journal_volume
203pub_type
杂志文章abstract::A Cl- channel protein of sarcoplasmic reticulum (SR) was purified by assaying the channel activity in a planar lipid bilayer system. The light fraction of SR vesicles was solubilized in CHAPS and fractioned by anion exchange, gel filtration, and affinity chromatography with concanavalin A. All fractions in each step w...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(91)90886-c
更新日期:1991-04-15 00:00:00
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(88)90569-4
更新日期:1988-02-29 00:00:00
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:1983-11-30 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:2016-04-22 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:1997-01-13 00:00:00
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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pub_type: 杂志文章
doi:10.1016/0006-291x(91)92113-x
更新日期:1991-12-31 00:00:00
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journal_title:Biochemical and biophysical research communications
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更新日期:1983-03-16 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(90)91217-g
更新日期:1990-09-14 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(91)91505-7
更新日期:1991-01-31 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(90)91198-2
更新日期:1990-09-14 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
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更新日期:1993-09-30 00:00:00
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:2001-10-19 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1997.6306
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journal_title:Biochemical and biophysical research communications
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:2020-09-10 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/s0006-291x(05)80870-8
更新日期:1990-12-31 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
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更新日期:1997-03-27 00:00:00
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journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1995.1505
更新日期:1995-04-06 00:00:00