Novel cancer chemopreventive effects of a flavonoid antioxidant silymarin: inhibition of mRNA expression of an endogenous tumor promoter TNF alpha.

abstract：:Prostaglandins (PGs) released by cultured rat Kupffer cells in response to stimulation with lipopolysaccharide (LPS) or ethanol were extracted from culture media, separated by HPLC and measured by radioimmunoassay. LPS (0.5-5 micrograms/ml) enhanced, after a 3-4 hrs lag period, the production of PGE2 (7-10 fold by 24 ...

journal_title：Biochemical and biophysical research communications

authors： Victorov AV,Hoek JB

更新日期：1995-10-13 00:00:00

abstract：:In order to study the relationship between insulin like growth factor-II (IGF-II) and interleukin-8 (IL-8) that are upregulated in psoriasis, we monitored IL-8 expression in IGF-II-treated human keratinocytes and explored the signaling pathways of IL-8 expression by IGF-II. IGF-II increased the IL-8 mRNA and protein l...

journal_title：Biochemical and biophysical research communications

authors： Kim HJ,Byun SJ,Kim TY

更新日期：2004-04-23 00:00:00

abstract：:The potential antiatherogenic actions of the angiotensin II receptor antagonist, losartan were investigated in apolipoprotein (apo) E deficient mice, an animal model with severe hypercholesterolemia and extensive atherosclerosis. In these animals accelerated atherosclerosis is associated with increased lipid peroxidat...

journal_title：Biochemical and biophysical research communications

authors： Keidar S,Attias J,Smith J,Breslow JL,Hayek T

更新日期：1997-07-30 00:00:00

abstract：:The Rh blood group antigens are carried by two distinct but homologous membrane proteins encoded by two closely related genes, RCHE and RHD. Rh50 glyco-protein is the membrane protein tightly associated with Rh polypeptides and is critical for expression of Rh antigens. The amino acid sequence and predicted membrane t...

journal_title：Biochemical and biophysical research communications

authors： Iwamoto S,Omi T,Yamasaki M,Okuda H,Kawano M,Kajii E

更新日期：1998-02-04 00:00:00

abstract：:Endothelin tightly bound to rabbit aortic strips and caused a prolonged vasoconstriction both in the presence and absence of extracellular Ca2+, although only partial constriction (20-30%) developed in the latter case, indicating that its action may not be limited to the opening of a calcium channel. The endothelin-in...

journal_title：Biochemical and biophysical research communications

authors： Sugiura M,Inagami T,Hare GM,Johns JA

更新日期：1989-01-16 00:00:00

abstract：:Mutations in mitochondrial DNA are one of the important causes of hearing loss. We report here the clinical, genetic, and molecular characterization of two Han Chinese pedigrees with maternally transmitted aminoglycoside-induced and nonsyndromic bilateral hearing loss. Clinical evaluation revealed the wide range of se...

journal_title：Biochemical and biophysical research communications

authors： Yuan H,Chen J,Liu X,Cheng J,Wang X,Yang L,Yang S,Cao J,Kang D,Dai P,Zhai S,Han D,Young WY,Guan MX

更新日期：2007-10-12 00:00:00

abstract：:High affinity iron uptake in yeast is carried out by a multicomponent system formed by the ferroxidase Fet3p and the iron permease Ftr1p. The currently accepted model predicts that Fet3p and Ftr1p are functionally associated, however, a structural interaction between these two proteins has not been proven yet. The met...

journal_title：Biochemical and biophysical research communications

authors： Bonaccorsi di Patti MC,Miele R,Eugenia Schininà M,Barra D

更新日期：2005-07-29 00:00:00

abstract：:We have previously demonstrated that homeodomain proteins, MEIS1 and PBXs, transactivate the PF4 gene through the novel regulatory element termed TME. This study focuses on Pbx regulating protein 1 (PREP1), a MEIS1 homolog protein, for its transcriptional activity in the PF4 promoter. PREP1 binds to the TME in HEL cel...

journal_title：Biochemical and biophysical research communications

authors： Okada Y,Matsuura E,Nagai R,Sato T,Watanabe A,Morita I,Doi T

更新日期：2003-05-23 00:00:00

abstract：:The hepatocyte growth factor is closely related to liver regeneration. And the c-met protooncogene is reported to encode a hepatocyte growth factor receptor. Little is known, however, about how the c-met gene is activated during liver regeneration. In this study, we demonstrated that expression of the c-met gene/hepat...

journal_title：Biochemical and biophysical research communications

authors： Ito T,Hayashi N,Horimoto M,Sasaki Y,Tanaka Y,Kaneko A,Fusamoto H,Kamada T

更新日期：1993-02-15 00:00:00

abstract：:Inflammation has multifaceted role in cancer progression including initiation, promotion and invasion by affecting the immune surveillance and associated signaling pathways. Inflammation facilitates the over-expression of cytokines, chemokines and growth factors involved in progression of different cancers including b...

journal_title：Biochemical and biophysical research communications

authors： Suman S,Sharma PK,Rai G,Mishra S,Arora D,Gupta P,Shukla Y

更新日期：2016-04-08 00:00:00

abstract：:Dehaloperoxidase (DHP) from Amphitrite ornata is the only heme-containing, hydrogen peroxide-dependent globin capable of oxidatively dehalogenating halophenols to yield the corresponding quinones. To ascertain that this enzymatic activity is intrinsic to DHP, we have cloned and expressed the enzyme in Escherichia coli...

journal_title：Biochemical and biophysical research communications

authors： Osborne RL,Taylor LO,Han KP,Ely B,Dawson JH

更新日期：2004-11-26 00:00:00

abstract：:Simple polyesters derived from poly(ethylene glycol)s and alpha, omega-dicarboxylic acids exhibit a broad range of activity in disrupting phospholipid membranes. This activity has been analyzed by measuring the release of liposome-encapsulated 5(6)-carboxy-fluorescein (CF). Comparison with an analogous monomeric surfa...

journal_title：Biochemical and biophysical research communications

authors： Regen SL,Jayasuriya N,Fabianowski W

更新日期：1989-03-15 00:00:00

abstract：:The ΔpH-dependent/Tat pathway is unique for using only the proton motive force for driving proteins transport across the thylakoid membrane in chloroplasts. 9-aminoacridine fluorescence quenching is widely used to monitor the ΔpH developed across the thylakoid membrane in the light. However, this method suffers from l...

journal_title：Biochemical and biophysical research communications

authors： Yang H,Pu X,Wang L,Liu L,Theg SM

更新日期：2017-04-22 00:00:00

abstract：:The biological function of hnRNP family proteins is widely diverse and involved in pre-mRNA processing, transcriptional regulation, recombination, and telomere maintenance. In the course of our study on the elucidation of biological functions of minisatellite DNA, we isolated several nuclear proteins that bind to the ...

journal_title：Biochemical and biophysical research communications

authors： Tanaka E,Fukuda H,Nakashima K,Tsuchiya N,Seimiya H,Nakagama H

更新日期：2007-06-29 00:00:00

abstract：:Abnormal proliferation of vascular smooth muscle cells is a key event in the formation of atherosclerotic plaques in humans. It has been suggested that modulation of platelet derived growth factor chain A (PDGF-A) gene expression may contribute to atherosclerosis. Using an 'in situ hybridization' technique the present...

journal_title：Biochemical and biophysical research communications

authors： Zwijsen RM,Japenga SC,Heijen AM,van den Bos RC,Koeman JH

更新日期：1992-08-14 00:00:00

abstract：:Stimulation of neutrophils with LTB(4) or PAF results in the production of a rapidly oscillating actin polymerization/depolymerization response. Treatment of neutrophils with inhibitors of PKC prior to stimulation with ligand resulted in a masking of the F-actin oscillations. Because myosin has been shown to be a subs...

journal_title：Biochemical and biophysical research communications

authors： Rengan R,Omann GM

更新日期：1999-08-27 00:00:00

abstract：:A single addition of ATP (20-1000 microM) to cultures of HL-60 cells resulted here in permanent, Ca(2+)-independent inhibition of cellular proliferation, evident 48 h following treatment. Extracellular ATP (ATPo) was maximally effective at 250 microM giving 90 +/- 1.5% growth inhibition. Up to a concentration of 250 m...

journal_title：Biochemical and biophysical research communications

authors： Seetulsingh-Goorah SP,Stewart BW

更新日期：1998-09-18 00:00:00

abstract：:Two genomic DNA clones encoding part of the mouse epidermal growth factor (EGF) receptor were isolated: an 8.0 kb insert that hybridized with the human EGF receptor kinase domain; and a 2.4 kb insert that mapped between the kinase and 3' autophosphorylation domains of the human EGF receptor gene. The 2.4 kb insert was...

journal_title：Biochemical and biophysical research communications

authors： Hung MC,Thompson KL,Chiu IM,Rosner MR

更新日期：1986-12-30 00:00:00

abstract：:Microinjection of purified transcriptionally active ribonucleoprotein (RNP) complex of vesicular stomatitis virus in vero cells resulted in the production and release of virus. Compared to the release of virus by cells treated with RNP in the presence of DEAE-dextran, the microinjection technique was highly efficient....

journal_title：Biochemical and biophysical research communications

authors： Thornton GB,Kopchick JJ,Stacey DW,Banerjee AK

更新日期：1983-11-15 00:00:00

abstract：:Dopamine transporter (DAT) plays a key role in terminating synaptic dopaminergic transmission. DAT acts exclusively on the plasma membrane of presynaptic dopaminergic neurons and DAT gene is an appropriate model for the study of dopaminergic neuron-specific regulation of gene activity. DAT represents an important targ...

journal_title：Biochemical and biophysical research communications

authors： Kouzmenko AP,Pereira AM,Singh BS

更新日期：1997-11-26 00:00:00

abstract：:Several human and rodent tumor cell lines were examined for the presence of integrin mRNAs by dot- and Northern-blot analysis. All tumor cells tested expressed mRNAs for alpha 5, alpha IIb, beta 1 and beta 3. The mRNA of beta 2 integrin was not detectable and that of alpha V integrin was found only in certain cells. N...

journal_title：Biochemical and biophysical research communications

authors： Chang SY,Chen YQ,Fitzgerald LA,Honn KV

更新日期：1991-04-15 00:00:00

abstract：:Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play important roles in brassinosteroid (BR)-induced antioxidant defense and enhancing the tolerance of plants to drought stress. The autophosphorylation of CCaMK is a key step for the activation of CCaMK, thus promoting substrate phosphorylation. H...

journal_title：Biochemical and biophysical research communications

authors： Liu L,Han T,Liu W,Han G,Di P,Yu X,Yan J,Zhang A

更新日期：2020-05-07 00:00:00

abstract：:Dysregulation of autophagy has been observed in obesity and type 2 diabetes. Salt-inducible kinase 2 (SIK2), a member of the AMPK-related kinase family, is downregulated in adipocytes from obese or insulin resistant individuals and was previously demonstrated to regulate autophagy in cancer and normal cell lines. The ...

journal_title：Biochemical and biophysical research communications

authors： Negoita F,Säll J,Morén B,Stenkula K,Göransson O

更新日期：2019-01-15 00:00:00

abstract：:In this communication, we document the self-assembly of heterologously expressed truncated human aromatase (CYP19) into nanometer scale phospholipids bilayers (Nanodiscs). The resulting P450 CYP19 preparation is stable and can tightly associate with the substrate androstenedione to form a nearly complete high-spin fer...

journal_title：Biochemical and biophysical research communications

authors： Grinkova YV,Denisov IG,Waterman MR,Arase M,Kagawa N,Sligar SG

更新日期：2008-07-25 00:00:00

abstract：:The self-induced endogenous elicitor isolated from potato having molecular weight 9200 was degraded with trifluoroacetic acid to determine the sugar composition. The requisite composition of the elicitor was estimated to be a 2:3:4:7 mixture of galactose, rhamnose, arabinose, and galacturonic acid. Furthermore, the st...

journal_title：Biochemical and biophysical research communications

authors： Monden T,Nakamura H,Murai A

更新日期：1995-10-13 00:00:00

abstract：:Plasma membrane fractions from mouse livers were examined for the presence of growth regulatory peptides. Both growth inhibitory and growth stimulatory factors were found on these membranes. The growth inhibitory component could be enriched by extractions with both dimethylmaleic anhydride and octylglucoside. The grow...

journal_title：Biochemical and biophysical research communications

authors： Lieberman MA

更新日期：1984-05-16 00:00:00

abstract：:Six missense mutations in human cardiac troponin I (cTnI) were recently found to cause restrictive cardiomyopathy (RCM). We have bacterially expressed and purified these human cTnI mutants and examined their functional and structural consequences. Inserting the human cTnI into skinned cardiac muscle fibers showed that...

journal_title：Biochemical and biophysical research communications

authors： Yumoto F,Lu QW,Morimoto S,Tanaka H,Kono N,Nagata K,Ojima T,Takahashi-Yanaga F,Miwa Y,Sasaguri T,Nishita K,Tanokura M,Ohtsuki I

更新日期：2005-12-23 00:00:00

abstract：:Homo-oligomerization via a coiled-coil (C-C) domain has been shown to be necessary for the promyelocytic leukemia (PML)-retinoic acid receptor-alpha (RARalpha) fusion protein to acquire oncogenic potential in acute promyelocytic leukemia. We show here that PML(DeltaC-C)-RARalpha, which contains a deletion in its C-C d...

journal_title：Biochemical and biophysical research communications

authors： Kim YE,Kim DY,Lee JM,Kim ST,Han TH,Ahn JH

更新日期：2005-05-13 00:00:00

abstract：:Understanding protein aggregation may hold important clues to understanding what goes wrong with protein folding in neurodegenerative disorders and in bioreactors in which proteins are overexpressed. Unfortunately, aggregates tend to be intractable to most standard methods of biochemical investigation. Thus, relativel...

journal_title：Biochemical and biophysical research communications

authors： Kundu B,Guptasarma P

更新日期：2002-04-26 00:00:00

abstract：:The activation of caspase-3 is a key surrogate marker for detecting apoptosis. To quantitate caspase-3 activity, we constructed a biosensor comprising a recombinant firefly luciferase containing a caspase-3 cleavage site. When apoptosis was induced, caspase-3 cleavage of the biosensor activated firefly luciferase by a...

journal_title：Biochemical and biophysical research communications

authors： Shi J,Zhang H,Fang L,Xi Y,Zhou Y,Luo R,Wang D,Xiao S,Chen H

更新日期：2014-10-03 00:00:00