Abstract:
:Microinjection of purified transcriptionally active ribonucleoprotein (RNP) complex of vesicular stomatitis virus in vero cells resulted in the production and release of virus. Compared to the release of virus by cells treated with RNP in the presence of DEAE-dextran, the microinjection technique was highly efficient. Microinjection in Xenopus oocytes also resulted in initiation of infection as shown by the synthesis of virus-specific proteins in the cell cytoplasm. It was further observed that RNP stripped of L protein but containing residual NS protein was capable of initiating virus production or protein synthesis when microinjected in vero cells or in oocytes, respectively. Since L and NS proteins are essential for in vitro transcription by RNP, these results suggest that a trace amount of L protein may remain bound to the RNP and a host factor may stimulate residual L activity in vivo.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Thornton GB,Kopchick JJ,Stacey DW,Banerjee AKdoi
10.1016/s0006-291x(83)80264-2subject
Has Abstractpub_date
1983-11-15 00:00:00pages
1160-7issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(83)80264-2journal_volume
116pub_type
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