Salt-inducible kinase 2 regulates TFEB and is required for autophagic flux in adipocytes.

abstract：:In RNA, function follows form. Mapping the surface of RNA molecules with chemical and enzymatic probes has revealed invaluable information about structure and folding. Hydroxyl radicals ((·)OH) map the surface of nucleic acids by cutting the backbone where it is accessible to solvent. Recent studies showed that a micr...

journal_title：Biochemical and biophysical research communications

authors： Schlatterer JC,Wieder MS,Jones CD,Pollack L,Brenowitz M

更新日期：2012-08-24 00:00:00

abstract：:Three immunoreactive prolactin proteins (24 Kd, 16 Kd, and 12 Kd) were identified in the rat brain using sodium dodecyl sulphate polyacrylamide gel electrophoresis, and western blot analyses. In male and female brains, the primary prolactin protein has a molecular weight of 24 Kd which is similar to that of pituitary ...

journal_title：Biochemical and biophysical research communications

authors： DeVito WJ

更新日期：1988-01-29 00:00:00

abstract：:We constructed a plasmid-based expression vector carrying the murine metallothionein gene promoter, the human preprorenin gene, the Tn5 neomycin phosphotransferase II gene, and a complete bovine papilloma virus genome. We were unsuccessful in cloning the preprorenin gene in E. coli when it was inserted into a plasmid ...

journal_title：Biochemical and biophysical research communications

authors： Weighous TF,Tarpley WG

更新日期：1987-03-13 00:00:00

abstract：:The epididymal maturation of spermatozoa is regulated by changes in the luminal ion concentration and the processing of the sperm surface membrane by several glycosidases and proteases. In the present study, we identified five novel protease inhibitors that are highly expressed in the mouse epididymis. Four of the pro...

journal_title：Biochemical and biophysical research communications

authors： Jalkanen J,Kotimäki M,Huhtaniemi I,Poutanen M

更新日期：2006-10-13 00:00:00

abstract：:Gastric cancer is one of the major causes of cancer death worldwide; however, the mechanism of carcinogenesis is complex and poorly understood. Long non-coding RNA HOTAIR (HOX transcript antisense RNA) recently emerged as a promoter of metastasis in various cancers including gastric cancer. Here we investigated the im...

journal_title：Biochemical and biophysical research communications

authors： Lee NK,Lee JH,Park CH,Yu D,Lee YC,Cheong JH,Noh SH,Lee SK

更新日期：2014-08-22 00:00:00

abstract：:Lipopolysaccharides (LPS) induces intrahepatic cholestasis and canalicular multispecific organic anion transporter (CMOAT/MRP2) plays a central role in hepatic bilirubin transport. This study examined the role of Kupffer cell in LPS-induced cholestasis. Rats were injected intravenously with LPS. Kupffer cells were ina...

journal_title：Biochemical and biophysical research communications

authors： Nakamura J,Nishida T,Hayashi K,Kawada N,Ueshima S,Sugiyama Y,Ito T,Sobue K,Matsuda H

更新日期：1999-02-05 00:00:00

abstract：:Retroviruses and retrotransposons depend on their host cells to complete their replication cycles. In many cases, the viral step of reverse transcription is blocked when host cells are arrested in their cell cycle and this block is only released when the host cell resumes division. It has previously been shown that a ...

journal_title：Biochemical and biophysical research communications

authors： Karst SM,Sadeghi N,Menees TM

更新日期：1999-01-27 00:00:00

abstract：:Thick filaments from some striated muscles are regulated by phosphorylation of myosin regulatory light chains (RLCs). A tarantula thick filament quasi-atomic model achieved by cryo-electron microscopy has advanced our understanding on how this regulation occurs. In native thick filaments, an asymmetric intramolecular ...

journal_title：Biochemical and biophysical research communications

authors： Sulbarán G,Biasutto A,Méndez F,Pinto A,Alamo L,Padrón R

更新日期：2020-03-26 00:00:00

abstract：:Nerve growth factor (NGF) elicits Akt translocation into the nucleus, where it phosphorylates nuclear targets. Here, we describe that Akt phosphorylation can promote the nuclear translocation of Akt and is necessary for its nuclear retention. Overexpression of Akt-K179A, T308A, S473A-mutant failed to show either nucle...

journal_title：Biochemical and biophysical research communications

authors： Xuan Nguyen TL,Choi JW,Lee SB,Ye K,Woo SD,Lee KH,Ahn JY

更新日期：2006-10-20 00:00:00

abstract：:Under standard conditions, in the presence of GTP, highly purified eIF-2 and Co-eIF-2 factor preparations efficiently stimulated AUG-codon dependent but not physiological mRNA-dependent Met-tRNAf binding to 40S ribosomes. Replacement of GTP by a nonhydrolyzable GTP analog, GMP-PNP, in the above system, gave significan...

journal_title：Biochemical and biophysical research communications

authors： Roy R,Nasrin N,Ahmad MF,Gupta NK

更新日期：1984-08-16 00:00:00

abstract：:Cysteine proteases have been identified in parasitic protozoa including the causative agent of Chagas' disease Trypanosoma cruzi. T. cruzi lysates subjected to substrate-containing SDS-polyacrylamide gel electrophoresis exhibit major bands of proteolytic activity in the 45-55 kDa molecular mass range (cruzipain activi...

journal_title：Biochemical and biophysical research communications

authors： Wiser MF,Lonsdale-Eccles JD,D'Alessandro A,Grab DJ

更新日期：1997-11-26 00:00:00

abstract：:The tissue inhibitors of metalloproteinases (TIMPs) are specific inhibitors of MMP enzymatic activity. However, TIMP-2 can promote the activation of pro-MMP-2 by MT1-MMP. This process is mediated by the formation of a complex between MT1-MMP, TIMP-2, and pro-MMP-2. Binding of TIMP-2 to active MT1-MMP also inhibits the...

journal_title：Biochemical and biophysical research communications

authors： Hernandez-Barrantes S,Shimura Y,Soloway PD,Sang QA,Fridman R

更新日期：2001-02-16 00:00:00

abstract：:The Wnt signaling pathway is crucial for development and disease. The regulation of Wnt protein trafficking is one of the pivotal issues in the Wnt research field. Here we performed a genetic screen in Drosophila melanogaster for genes involved in Wingless/Wnt secretion, and identified the p24 protein family members B...

journal_title：Biochemical and biophysical research communications

authors： Li X,Wu Y,Shen C,Belenkaya TY,Ray L,Lin X

更新日期：2015-08-07 00:00:00

abstract：:Liver regeneration is controlled by multiple signaling pathways induced by a variety of growth factors, hormones, and cytokines. Here we report that protein kinase B (PKB)/Akt, part of a key cell survival signaling pathway, is markedly activated after partial hepatectomy (PHX). The antiapoptotic protein Bad, a downstr...

journal_title：Biochemical and biophysical research communications

authors： Hong F,Nguyen VA,Shen X,Kunos G,Gao B

更新日期：2000-12-29 00:00:00

abstract：:Spermine and spermidine are present in rat liver mitochondria at the concentrations of 3.5 and 1.8 nanomoles/mg protein, respectively. Addition of 50 microM Ca2+ and 1 mM Pi to respiring mitochondria induces, concomitantly with mitochondrial swelling and Mg2+ efflux, a consistent release of polyamines, which is either...

journal_title：Biochemical and biophysical research communications

authors： Tassani V,Campagnolo M,Toninello A,Siliprandi D

更新日期：1996-09-24 00:00:00

abstract：:Tankyrases (TNKS and TNKS2) are enzymes that catalyze poly-ADP-ribosylation (PARsylation) of their target proteins. Tankyrase-mediated PARsylation plays critical regulatory roles in cell signaling, particularly in the Wnt/β-catenin pathway. The sterile alpha motif (SAM) domain in tankyrases mediates their oligomerizat...

journal_title：Biochemical and biophysical research communications

authors： Fan C,Yarravarapu N,Chen H,Kulak O,Dasari P,Herbert J,Yamaguchi K,Lum L,Zhang X

更新日期：2018-09-10 00:00:00

abstract：:Primary mouse embryo fibroblasts passed in culture go through a period of declining growth rate and then after a period of minimal proliferation begin to grow again, generating, under appropriate conditions, an immortal 3T3 line. The 3T3 cells, but not the primary mouse embryo fibroblasts, are able to synthesize and t...

journal_title：Biochemical and biophysical research communications

authors： Edwards DR,Parfett CL,Smith JH,Denhardt DT

更新日期：1987-08-31 00:00:00

abstract：:In this study, we demonstrate that in addition to T lymphocytes, human naïve eosinophils and the differentiated eosinophil-like cell line, AML14.3D10 express CCR8 and respond to CCL1 through CCR8 engagement. The responsiveness of cells was dependent on maturation stage, since CCL1 induced pronounced chemotaxis only in...

journal_title：Biochemical and biophysical research communications

authors： Karlsson AK,Walles K,Bladh H,Connolly S,Skrinjar M,Rosendahl A

更新日期：2011-04-22 00:00:00

abstract：:Tissue factor (TF) is an initiation factor for blood coagulation and its expression is induced on endothelial cells during inflammatory or immune responses. We designed an antisense oligodeoxynucleotide (AS-1/TF) for rat TF and studied its effect on hepatic ischemic reperfusion injury. AS-1/TF was delivered intravenou...

journal_title：Biochemical and biophysical research communications

authors： Nakamura K,Kadotani Y,Ushigome H,Akioka K,Okamoto M,Ohmori Y,Yaoi T,Fushiki S,Yoshimura R,Yoshimura N

更新日期：2002-09-27 00:00:00

abstract：:Resistin, an adipocyte secreted cysteine rich hormone has been implicated as molecular link between obesity and type 2 diabetes in a murine model. Although, at the protein level mouse and human resistin show remarkable similarities with respect to conserved cysteine residues, the physiological role of human resistin i...

journal_title：Biochemical and biophysical research communications

authors： Raghu P,Ghosh S,Soundarya K,Haseeb A,Aruna B,Ehtesham NZ

更新日期：2004-01-16 00:00:00

abstract：:The discovery of regulation relationship of protein interactions is crucial for the mechanism research in signaling network. Bioinformatics methods can be used to accelerate the discovery of regulation relationship between protein interactions, to distinguish the activation relations from inhibition relations. In this...

journal_title：Biochemical and biophysical research communications

authors： Liu W,Xie H

更新日期：2013-10-25 00:00:00

abstract：:Calmodulin activation of target enzymes depends on the interaction between calmodulin hydrophobic regions and some enzyme areas. The Ca2+ induced exposure of calmodulin hydrophobic sites was studied by means of 2-p-toluidinylnaphthalene-6-sulfonate, a fluorescent probe. Scatchard and Job plots showed that the calmodul...

journal_title：Biochemical and biophysical research communications

authors： Follenius A,Gerard D

更新日期：1984-03-30 00:00:00

abstract：:The human cDNA encoding a novel protein serine/threonine kinase most closely related to p70 S6 kinase was isolated from the human erythroleukemia cDNA library and termed p70(S6Kbeta). p70(S6Kbeta) has 67% amino acid identity in overall sequence with human p70(S6K), and the potential phosphorylation sites of p70(S6K) a...

journal_title：Biochemical and biophysical research communications

authors： Saitoh M,ten Dijke P,Miyazono K,Ichijo H

更新日期：1998-12-18 00:00:00

abstract：:In Dictyostelium discoideum, a novel type of RNA (dutA RNA), which is untranslatable, cytosolic and 1.3 kb in size, appears specifically after the aggregation stage [Yoshida, H. et al. (1994) Nucleic Acids Res. 22, 41-46]. We here show that the dutA gene is transcribed by RNA polymerase II, based on its alpha-amanitin...

journal_title：Biochemical and biophysical research communications

authors： Kumimoto H,Yoshida H,Okamoto K

更新日期：1995-11-02 00:00:00

abstract：:NF-kappaB family of transcription factors are involved in numerous cellular processes, including differentiation, proliferation, and inflammation. It was reported that hydroxycinnamic acid derivatives (HADs) are inhibitors of NF-kappaB activation. Rice bran oil contains a lot of phytosteryl ferulates, one of HADs. We ...

journal_title：Biochemical and biophysical research communications

authors： Nagasaka R,Chotimarkorn C,Shafiqul IM,Hori M,Ozaki H,Ushio H

更新日期：2007-06-29 00:00:00

abstract：:The classified sediment with ethanol from sera of nude mice and humans showed a disturbing effect on L1210 cells in vitro and a lifesaving effect on L1210 cell-bearing mice in vivo. This factor was purified more than 2300-fold to a specific activity of approximately 1 X 10(5) U/mg by ethanol classified precipitation, ...

journal_title：Biochemical and biophysical research communications

authors： Sakagami Y,Manabe H

更新日期：1984-04-30 00:00:00

abstract：:Elafin, a human skin derived inhibitor of human leukocyte elastase, was tested for inhibitory activity against proteinase 3, an elastin degrading proteinase of neutrophils. The inhibitory activity of elafin was compared with antileukoprotease and eglin C. Elafin proved to be a potent inhibitor of elastin-FITC degradat...

journal_title：Biochemical and biophysical research communications

authors： Wiedow O,Lüademann J,Utecht B

更新日期：1991-01-15 00:00:00

abstract：:Mutant 6B7 of Salmonella enterica serovar Typhimurium has a transposon inserted in the putative transporter gene yjeH and shows a more-than-fourfold reduction in resistance to ceftriaxone. In this report we have used proteomic analysis to compare outer membrane protein profiles between this mutant and its parental str...

journal_title：Biochemical and biophysical research communications

authors： Hu WS,Lin YH,Shih CC

更新日期：2007-09-28 00:00:00

abstract：:The potential association of CD2AP with the adherens junction protein E-cadherin, co-localization with the actin cytoskeleton, and involvement in cell migration was investigated in cultured rat gastric mucosal cells. In stationary cells, CD2AP was localized perinuclearly while E-cadherin was expressed along cell-cell ...

journal_title：Biochemical and biophysical research communications

authors： Mustonen H,Lepistö A,Lehtonen S,Lehtonen E,Puolakkainen P,Kivilaakso E

更新日期：2005-07-01 00:00:00

abstract：:The binding of leptin to hypothalamic neurons elicits inhibition of orexigenic NPY/AgRP neurons and stimulation of anorexigenic POMC/CART neurons. Projections of serotonergic neurons onto POMC neurons suggest that leptin and serotonin converge onto POMC neurons to regulate body weight. We probed the interaction of the...

journal_title：Biochemical and biophysical research communications

authors： Wang B,Chehab FF

更新日期：2006-12-15 00:00:00