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A bacteriophage transcription terminator permits the cloning of a mammalian expression vector carrying the human preprorenin gene in E. coli.

Abstract：

:We constructed a plasmid-based expression vector carrying the murine metallothionein gene promoter, the human preprorenin gene, the Tn5 neomycin phosphotransferase II gene, and a complete bovine papilloma virus genome. We were unsuccessful in cloning the preprorenin gene in E. coli when it was inserted into a plasmid vector 3' to the metallothionein gene promoter. This result was consistent with our previous data suggesting that expression of the preprorenin gene is toxic to E. coli (Weighous, T.F. et al; (1986) Gene 45: 121-129). We then inserted a DNA fragment from bacteriophage lambda carrying the t1 transcriptional terminator between the promoter and the preprorenin gene; this vector was stably maintained in E. coli. Prior to introducing the vector DNA into mouse cells for expression studies the terminator was removed. Mouse cells carrying the vector DNA secreted high levels of human prorenin for greater than six weeks.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Weighous TF,Tarpley WG

doi

10.1016/0006-291x(87)91395-7

subject

Has Abstract

pub_date

1987-03-13 00:00:00

pages

593-9

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(87)91395-7

journal_volume

143

pub_type

杂志文章

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