Glucocorticoid suppresses the canonical Wnt signal in cultured human osteoblasts.

abstract：:To investigate the possible mechanism of gene transcription changes induced by magnetic field (MF), we examined the DNA binding behavior of the transcription factor cyclic-AMP responsive element binding protein (CREB) in HL60 cells after exposure to a 0.1mT 50-Hz extremely low frequency (ELF) sinusoidal MF by a gel sh...

journal_title：Biochemical and biophysical research communications

authors： Zhou J,Yao G,Zhang J,Chang Z

更新日期：2002-08-30 00:00:00

abstract：:We determined the cDNA sequence for neurocalcin, a novel calcium-binding protein in bovine brain. This clone (pCalN) has 582 nucleotides in the open reading frame including the termination codon TGA, 11 nucleotides of the 5' leader and 1251 nucleotides of the 3' noncoding region. The deduced amino acid sequence reveal...

journal_title：Biochemical and biophysical research communications

authors： Okazaki K,Watanabe M,Ando Y,Hagiwara M,Terasawa M,Hidaka H

更新日期：1992-05-29 00:00:00

abstract：:The tetracycline-regulated gene expression system has been widely used in mice to turn a transgene on and off in a target organ, but with only limited success. We developed an advanced system in which a Tet-off regulation unit was integrated into the ROSA26 locus and became active after Cre-mediated excision of the ne...

journal_title：Biochemical and biophysical research communications

authors： Miyazaki S,Miyazaki T,Tashiro F,Yamato E,Miyazaki J

更新日期：2005-12-16 00:00:00

abstract：:High mobility group box 1 (HMGB1) was recently shown to be an important extracellular mediator of severe vascular inflammatory disease, sepsis. Lysozyme (LYZ) has been shown to bind to bacterial lipopolysaccharide (LPS) and have a potential for playing a role in the therapy of inflammatory diseases. However, the effec...

journal_title：Biochemical and biophysical research communications

authors： Lee W,Park EJ,Kwak S,Kim Y,Na DH,Bae JS

更新日期：2015-04-17 00:00:00

abstract：:CDglyTK fusion suicide gene has been well characterized to effectively kill tumor cells. However, the exact mechanism and downstream target genes are not fully understood. In our study, we found that CDglyTK/prodrug treatment works more efficiently in p53 wild-type (HONE1) cells than in p53 mutant (CNE1) cells. We the...

journal_title：Biochemical and biophysical research communications

authors： Yu Z,Wang H,Zhang L,Tang A,Zhai Q,Wen J,Yao L,Li P

更新日期：2009-09-04 00:00:00

abstract：:The exposure of hydrophobic sites on calmodulin, skeletal muscle troponin C and their tryptic fragments was investigated using Phenyl-Sepharose chromatography. A strong binding of both proteins and their fragments corresponding to the NH2-terminal halves of polypeptide chain of respective proteins in the presence of c...

journal_title：Biochemical and biophysical research communications

authors： Brzeska H,Szynkiewicz J,Drabikowski W

更新日期：1983-08-30 00:00:00

abstract：:Lysates of rabbit reticulocytes and other mammalian cells are known to contain an activity which binds with high specificity ppp(A2'p)3A,3'-[32P]pCp. The binding activity shows a marked dependence on preincubation of lysates at different temperatures (4 degrees C - 45 degrees C). For example, binding was increased 50%...

journal_title：Biochemical and biophysical research communications

authors： Wu JM,Eslami B,Semproni AR

更新日期：1983-11-30 00:00:00

abstract：:HIV-1 Vpr is a protein with multiple functions. It has been suggested that such pleiotropic effects by a viral protein may be mediated by its association with viral and cellular proteins or through modulation of expression of specific cellular genes. To address this, we used cDNA microarray techniques to analyze the r...

journal_title：Biochemical and biophysical research communications

authors： Janket ML,Manickam P,Majumder B,Thotala D,Wagner M,Schafer EA,Collman RG,Srinivasan A,Ayyavoo V

更新日期：2004-02-20 00:00:00

abstract：:Interleukin 12 has demonstrated a wide spectrum of bioactivity on T and NK cells both in vitro and in vivo. Therapeutic potential of these activities has been suggested by studies in murine tumor, viral and microbial models of disease. We have investigated the in vivo effect of rhlL-12 in cynomolgus monkeys treated wi...

journal_title：Biochemical and biophysical research communications

authors： Bree AG,Schlerman FJ,Kaviani MD,Hastings RC,Hitz SL,Goldman SJ

更新日期：1994-11-15 00:00:00

abstract：:Surfactant convertase is required for conversion of heavy density (H) natural surfactant to light density (L) subtype during cycling in vitro, a technique that reproduces surfactant metabolism. To study mechanisms of H to L conversion, we prepared liposomes of dipalmitoylphosphatidylcholine (DPPC) and phosphatidylglyc...

journal_title：Biochemical and biophysical research communications

authors： Dhand R,Sharma VK,Teng AL,Krishnasamy S,Gross NJ

更新日期：1998-03-27 00:00:00

abstract：:Laminins, the major basement membrane glycoproteins, are composed of three subunits. We identified a splice variant of the human laminin alpha4 subunit transcript containing 21 extra nucleotides. A heptapeptide sequence, MDCPTIS, was inserted close to the two cysteine residues possibly involved in the intersubunit dis...

journal_title：Biochemical and biophysical research communications

authors： Hayashi Y,Kim KH,Fujiwara H,Shimono C,Yamashita M,Sanzen N,Futaki S,Sekiguchi K

更新日期：2002-12-06 00:00:00

abstract：:It is known that NGF-responsive cells bind NGF at cell surface receptors in a specific and saturable fashion and there are two separate kinds of receptor-ligand binding interactions as judged by Rosenthal analyses. Following isolation of nerve growth factor receptors from embryonic chicken sensory ganglia, rat pheochr...

journal_title：Biochemical and biophysical research communications

authors： Lyons CR,Stach RW,Perez-Polo JR

更新日期：1983-08-30 00:00:00

abstract：:Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of cardiovascular diseases and are modified in response to a variety of stimuli such as bioactive peptides, cytokines and/or grown factors. In this study, we demonstrated that angiotensin II (Ang II) induces a time- and dose-dependent increase...

journal_title：Biochemical and biophysical research communications

authors： Jiménez E,Pérez de la Blanca E,Urso L,González I,Salas J,Montiel M

更新日期：2009-03-20 00:00:00

abstract：:Binding of [3H]-staurosporine to different protein kinases was time-dependent, reversible and saturable. Scatchard analysis of saturation isotherms indicated one class of binding sites for [3H]-staurosporine with dissociation constants (KD) of 9.6, 2.0, 3.0 and 7.4 nM for protein kinase C, cAMP-dependent protein kinas...

journal_title：Biochemical and biophysical research communications

authors： Herbert JM,Seban E,Maffrand JP

更新日期：1990-08-31 00:00:00

abstract：:The specificity of the endoglycanase associated with the bacteriophage phi SK1 particles was tested on the native capsular polysaccharide produced by Klebsiella pneumoniae serotype SK1 and on three chemically modified polymers derived from it. The primary structure of the SK1 capsular polysaccharide is: [formula: see ...

journal_title：Biochemical and biophysical research communications

authors： Cescutti P,Paoletti S

更新日期：1994-02-15 00:00:00

abstract：:Multiple system atrophy is a neurodegenerative disease caused by abnormal α-synuclein (α-syn) accumulation in oligodendrocytes and neurons. We previously demonstrated that transgenic (Tg) mice that selectively overexpressed human α-syn in oligodendrocytes exhibited neuronal α-syn accumulation. Microtubule β-III tubuli...

journal_title：Biochemical and biophysical research communications

authors： Ito H,Nakayama K,Jin C,Suzuki Y,Yazawa I

更新日期：2012-11-23 00:00:00

abstract：:The nucleotide analogue 5-p-fluorosulfonylbenzoyladenosine reacts with rat liver microsomal 3-hydroxy-3-methylglutaryl-CoA reductase kinase, causing a rapid loss of the AMP activation capacity and a slower inactivation of the catalytic activity. The rate constant for loss of AMP activation is eleven times higher (K1 =...

journal_title：Biochemical and biophysical research communications

authors： Ferrer A,Caelles C,Hegardt FG

更新日期：1987-11-13 00:00:00

abstract：:We have identified a 35-amino acid antibiotic cecropin secreted by an established mosquito cell line. C7-10 cells from the vector mosquito, Aedes albopictus, were incubated with heat-killed Escherichia coli, and materials secreted into the cell culture supernatant were recovered by acid precipitation. Following batch ...

journal_title：Biochemical and biophysical research communications

authors： Sun D,Eccleston ED,Fallon AM

更新日期：1998-08-19 00:00:00

abstract：:In isolated rat pancreatic acini, protein expression of RhoA and Rho-associated kinase, ROCK-II, and the formation of immunocomplex of RhoA with ROCK-II were enhanced by CCK-8, carbachol, and the phorbol ester TPA. The ROCK-specific inhibitor, Y-27632, did not alter basal amylase secretion, whereas it potentiated CCK-...

journal_title：Biochemical and biophysical research communications

authors： Kusama K,Nozu F,Awai T,Tanaka S,Honma I,Tsunoda Y,Mitamura K

更新日期：2003-05-30 00:00:00

abstract：:To study the role of the cytoplasmic domain and particularly the tyrosine residues of the erythropoietin receptor (EpoR) in erythroid differentiation of human primary stem cells, we infected cord blood-derived CD34+ cells with retroviruses encoding chimeric receptors containing the extracellular domain of the prolacti...

journal_title：Biochemical and biophysical research communications

authors： Fichelson S,Chrétien S,Rokicka-Piotrowicz M,Bouhanik S,Gisselbrecht S,Mayeux P,Lacombe C

更新日期：1999-03-24 00:00:00

abstract：:Leda-1/Pianp is a type I transmembrane protein expressed by CNS cells, murine melanoma cell line B16F10 and rat liver sinusoidal endothelial cells. The early steps of posttranslational modifications of Leda-1/Pianp have been described to include glycosylation and processing by proprotein convertases. Here, we comprehe...

journal_title：Biochemical and biophysical research communications

authors： Biswas S,Adrian M,Weber J,Evdokimov K,Winkler M,Géraud C

更新日期：2016-09-02 00:00:00

abstract：:The acidic leucine-rich nuclear phosphoprotein 32B (ANP32B) is a member of a conserved superfamily of nuclear proteins whose functions are largely unknown. In our previous work, ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. In this work, ...

journal_title：Biochemical and biophysical research communications

authors： Yu Y,Shen SM,Zhang FF,Wu ZX,Han B,Wang LS

更新日期：2012-07-13 00:00:00

abstract：:Calreticulin has multiple functions, diverse cellular locations, and putative isoforms. It likely maintains integrin avidity by binding alpha integrin cytoplasmic tails and is a surface lectin which triggers cell spreading. In the present study, we have immunocaptured a cell surface complex from B16 mouse melanoma cel...

journal_title：Biochemical and biophysical research communications

authors： Zhu Q,Zelinka P,White T,Tanzer ML

更新日期：1997-03-17 00:00:00

abstract：:The Rho-associated coiled-coil kinase (ROCK) family of proteins, including ROCK1 and ROCK2, are key regulators of actin and intermediate filament morphology. The newly discovered ROCK substrate Tubulin polymerization promoting protein 1 (TPPP1) promotes microtubule polymerization and inhibits the activity of Histone d...

journal_title：Biochemical and biophysical research communications

authors： Schofield AV,Gamell C,Bernard O

更新日期：2013-07-12 00:00:00

abstract：:One of the monocytic cell colony stimulating factors produced by thymic myoid cells, a 100 kDa factor, was purified by reversed phase HPLC and found to be homologous to the secreted form of proteoglycan 1 (biglycan) core protein. This biglycan associated colony stimulating factor did not carry an immunological motif o...

journal_title：Biochemical and biophysical research communications

authors： Kamo I,Kikuchi A,Nonaka I,Yamada E,Kondo J

更新日期：1993-09-15 00:00:00

abstract：:Matrix metalloproteinases (MMP-2 and -9) play an important role in the tumor metastasis through cleavage of proinflammatory cytokines. Violacein a small molecule produced by Chromobacterium violaceum and has been implicated with anti-cancer effects. In this study we investigated the molecular basis of violacein mediat...

journal_title：Biochemical and biophysical research communications

authors： Platt D,Amara S,Mehta T,Vercuyssee K,Myles EL,Johnson T,Tiriveedhi V

更新日期：2014-12-05 00:00:00

abstract：:PRA1 (prenylated Rab acceptor) is a general regulator of Rab proteins, while RILP (Rab interacting lysosomal protein) is a specific effector for Rab7. It has been shown that PRA1 interacts with Rab proteins and with VAMP2. Therefore PRA1 is probably an important factor for membrane traffic, linking together the functi...

journal_title：Biochemical and biophysical research communications

authors： Bucci C,De Gregorio L,Bruni CB

更新日期：2001-08-31 00:00:00

abstract：:Methotrexate (MTX) is a dihydrofolate reductase (DHFR) inhibitor widely used for treating human cancers, and overexpression of histone deacetylase (HDAC) is usually found in tumors. HDAC inhibitors (HDACi) can reactivate tumor suppressor genes and serve as potential anti-cancer drugs. In this study, we found that MTX ...

journal_title：Biochemical and biophysical research communications

authors： Yang PM,Lin JH,Huang WY,Lin YC,Yeh SH,Chen CC

更新日期：2010-01-15 00:00:00

abstract：:Stretch activation of cation-permeable channels may be an important proximal sensory mechanism in mechanotransduction. As actin filaments may mediate cellular responses to changes of the mechanical properties of the substrate and regulate stretch-induced calcium transients, we examined the role of actin filaments and ...

journal_title：Biochemical and biophysical research communications

authors： Wu Z,Wong K,Glogauer M,Ellen RP,McCulloch CA

更新日期：1999-08-02 00:00:00

abstract：:Two alternatively spliced Caenorhabditis elegans G protein-coupled receptors, T19F4.1a and T19F4.1b, were cloned and functionally characterized. The T19F4.1b receptor protein is 30 amino acids longer than T19F4.1a, and the difference in amino acid constitution is exclusively conferred to the intracellular C-terminal r...

journal_title：Biochemical and biophysical research communications

authors： Mertens I,Meeusen T,Janssen T,Nachman R,Schoofs L

更新日期：2005-05-13 00:00:00