Novel regulation of protein kinase C-η.

abstract：:Recent studies revealed dramatic changes in circadian clock genes' expression during the perinatal period. In this study, we characterized DNA methylation for three clock genes mPer1, mPer2, and mCry1 at their selected promoter regions during development. Results for the suprachiasmatic nucleus (SCN) and liver (at emb...

journal_title：Biochemical and biophysical research communications

authors： Ji Y,Qin Y,Shu H,Li X

更新日期：2010-01-22 00:00:00

abstract：:Two Chinese hamster ovary cell clones resistant to okadaic acid (OA) were isolated. The OA-resistance was associated with resistance to colchicine, Vinca alkaloids and inhibitors of DNA topoisomerase (topo) II. Drug accumulation assays showed that the intracellular levels of OA, vinblastine and vincristine, but not th...

journal_title：Biochemical and biophysical research communications

authors： Tohda H,Yasui A,Yasumoto T,Nakayasu M,Shima H,Nagao M,Sugimura T

更新日期：1994-09-15 00:00:00

abstract：:Human lung carcinoma cells (A549) were oxidatively stressed with mildly-toxic or non-toxic amounts of hydrogen peroxide (H2O2, 0.1 mM to 120 mM) for 5 min. Hydrogen peroxide exposure resulted in a dose dependent inhibition of binding (pH 7) of the thiol reagent iodoacetic acid (IAA) to a 38 kDa cell protein. Incubatio...

journal_title：Biochemical and biophysical research communications

authors： Brodie AE,Reed DJ

更新日期：1987-10-14 00:00:00

abstract：:Antibody against actin isolated from a human EBV-transformed lymphoblastoid B-cell line exerted an inhibitory effect on in vitro IgM secretion by a different lymphoblastoid B-cell line, LA350. This effect was dose dependent showing from 24-40% inhibition at a dilution of 1:100 and 68-80% inhibition at a dilution of 1:...

journal_title：Biochemical and biophysical research communications

authors： Rosenblatt HM,Green CG,McClure JE,Shearer WT

更新日期：1986-10-15 00:00:00

abstract：:p73 is one of the p53 family members which are transcription factors involved in the regulation of cell proliferation, apoptosis, and differentiation. In this study, we cloned the p73 cDNA from zebrafish ovary RNA. The consensus open reading frame (1923bp) encodes a polypeptide of 640 amino acids which shares 70-95% i...

journal_title：Biochemical and biophysical research communications

authors： Pan H,Dung HN,Hsu HM,Hsiao KM,Chen LY

更新日期：2003-07-25 00:00:00

abstract：:Neurogenin-3 (ngn-3) expression is critical for endocrine development in the developing pancreas. We found that when ngn-3 was inhibited in an E11.5 pancreas, using either morpholino antisense or siRNA, it led to a significant decrease in endocrine differentiation after seven days in culture. Endocrine differentiation...

journal_title：Biochemical and biophysical research communications

authors： Prasadan K,Tulachan S,Guo P,Shiota C,Shah S,Gittes G

更新日期：2010-06-11 00:00:00

abstract：:Methiocarb, an aromatic-alkyl sulfide insecticide was enzymatically oxidized into its sulfoxide by microsomes from soybean cotyledons. No further oxidation into sulfone was detected. Distribution of the sulfoxidase activity was studied in soybean seedlings and found maximal in cotyledons. Subcellular fractionation of ...

journal_title：Biochemical and biophysical research communications

authors： Blee E,Durst F

更新日期：1986-03-28 00:00:00

abstract：:A 1763-bp cDNA for rat liver bilirubin UDP-glucuronosyltransferase (UDPGT) was isolated. Bilirubin UDPGT activity was demonstrated by transfection of the pcDL1 vector carrying the cDNA into COS7 monkey kidney cells. The cDNA shares an identical 913-bp sequence (corresponding to the C-terminal 247 amino acid residues) ...

journal_title：Biochemical and biophysical research communications

authors： Sato H,Koiwai O,Tanabe K,Kashiwamata S

更新日期：1990-05-31 00:00:00

abstract：:This article investigated the effects of pramipexole on myocardial ischemia reperfusion (I/R) injury and its underlying mechanisms. We utilized an in vivo mouse model of myocardial I/R injury and an in vitro H9c2 cell model of hypoxia/reoxygenation (H/R) injury. Pramipexole pretreatment in male C57BL/6 mice significan...

journal_title：Biochemical and biophysical research communications

authors： Mo Y,Tang L,Ma Y,Wu S

更新日期：2016-05-13 00:00:00

abstract：:A recently described endo-exodeoxyribonuclease activity (1) was determined to interact preferentially with the origin/enhancer region and the 5' end of the late promoter region of the SV40 mini chromosome. The enzymatic activity has no preferential site of interaction with naked DNA, which suggests, the chromatin orga...

journal_title：Biochemical and biophysical research communications

authors： Quarless SA

更新日期：1985-01-31 00:00:00

abstract：:Hepatocytes from human bone marrow-derived mesenchymal stem cells (hBM-MSCs) are expected to be a useful source for cell transplantation. However, relatively low efficiency and repeatability of hepatic differentiation of human BM-MSCs remains an obstacle for clinical translation. Hepatocyte nuclear factor 4 alpha (HNF...

journal_title：Biochemical and biophysical research communications

authors： Hu X,Xie P,Li W,Li Z,Shan H

更新日期：2016-09-16 00:00:00

abstract：:The effects of insulin on the steady-state level of human insulin receptor (hIR) mRNA were examined in the HepG2 human liver cell line using Northern blot analysis of either total cellular or poly(A)+ RNA. In control cells, up to six (4.5, 5.2, 7.4, 8.5, 9.4 and 10.8 kb) hybridizable species of hIR mRNA were identifie...

journal_title：Biochemical and biophysical research communications

authors： Rohilla AM,Anderson C,Wood WM,Berhanu P

更新日期：1991-03-15 00:00:00

abstract：:Wet elastoidin spicules (fish fin rays) yield low-angle meridional X-ray diffraction patterns which resemble those from tendons. However, when the spicule dries the meridian splits into the arms of a diagonal cross (sometimes only one arm appears). Of the possible explanations we reject shearing of the axial arrangeme...

journal_title：Biochemical and biophysical research communications

authors： Hukins DW,Woodhead-Galloway J,Knight DP

更新日期：1976-12-20 00:00:00

abstract：:The second leader exon and the relative promoter of the mouse liver/bone/kidney-type alkaline phosphatase gene were identified and characterized. The transcription initiation site was determined by S1 mapping analysis. The differential expression of the two alternatively spliced transcripts was assessed in cell lines ...

journal_title：Biochemical and biophysical research communications

authors： Studer M,Terao M,Gianni M,Garattini E

更新日期：1991-09-30 00:00:00

abstract：:Breath isoprene accounts for most of the hydrocarbon removal via exhalation and is thought to serve as a non-invasive indicator for assaying several metabolic effects in the human body. The primary objective of this paper is to introduce a novel working hypothesis with respect to the endogenous source of this compound...

journal_title：Biochemical and biophysical research communications

authors： King J,Mochalski P,Unterkofler K,Teschl G,Klieber M,Stein M,Amann A,Baumann M

更新日期：2012-07-06 00:00:00

abstract：:Hydrogen peroxide, tert-butyl hydroperoxide, cumene hydroperoxide, and 3-chloroperoxybenzoic acid (CPB) and 15-HPETE relaxed, in a concentration dependent manner rat aortic rings contracted with PGF2 alpha (1 X 10(-5)). Relaxation is not inhibited by either indomethacin (2 X 10(-5) M), a cyclo-oxygenase inhibitor or e...

journal_title：Biochemical and biophysical research communications

authors： Thomas G,Ramwell P

更新日期：1986-08-29 00:00:00

abstract：:Using the patch-clamp technique, we determined that 1-15 mM extracellular acetylcholine reduced whole-cell n-type K+ currents in human peripheral blood lymphocytes and accelerated their inactivation. The percentage increase in K+ channel inactivation rate and the degree of drug induced block were independent of membra...

journal_title：Biochemical and biophysical research communications

authors： Gáspár R Jr,Varga Z,Bene L,Marcheselli F,Pieri C,Damjanovich S

更新日期：1996-09-13 00:00:00

abstract：:A novel nucleic acid analogue (2Cl-C.OXT-A) significantly stimulated tube formation of human umbilical endothelial cells (HUVEC). Its maximum potency at 100muM was stronger than that of vascular endothelial growth factor (VEGF), a positive control. At this concentration, 2Cl-C.OXT-A moderately stimulated proliferation...

journal_title：Biochemical and biophysical research communications

authors： Tsukamoto I,Sakakibara N,Maruyama T,Igarashi J,Kosaka H,Kubota Y,Tokuda M,Ashino H,Hattori K,Tanaka S,Kawata M,Konishi R

更新日期：2010-09-03 00:00:00

abstract：:Five commonly used cationic liposome formulations were tested for their ability to deliver DNA to established subcutaneous human tumor xenografts in SCID mice. Liposomes were complexed with a mammalian expression plasmid containing the bacterial beta-galactosidase gene and delivered to tumors by direct injection. The ...

journal_title：Biochemical and biophysical research communications

authors： Egilmez NK,Iwanuma Y,Bankert RB

更新日期：1996-04-05 00:00:00

abstract：:Patient-derived xenografts (PDXs) are widely recognised as a more physiologically relevant preclinical model than standard cell lines, but are expensive and low throughput, have low engraftment rate and take a long time to develop. Our newly developed conditional reprogramming (CR) technology addresses many PDX drawba...

journal_title：Biochemical and biophysical research communications

authors： Mondal AM,Ma AH,Li G,Krawczyk E,Yuan R,Lu J,Schlegel R,Stamatakis L,Kowalczyk KJ,Philips GK,Pan CX,Liu X

更新日期：2019-09-10 00:00:00

abstract：:The microtubule motor kinesin-1 is responsible for the nuclear positioning during myogenesis. Here we show that the coiled-coil stalk/tail domain containing the kinesin light chain (KLC) binding sites targets to the perinuclear region like endogenous Kif5b, while the globular tail domain cannot. To investigate which f...

journal_title：Biochemical and biophysical research communications

authors： Wang Z,Xue W,Li X,Lin R,Cui J,Huang JD

更新日期：2013-03-08 00:00:00

abstract：:Mutants of mouse T-lymphoma S49 cells lacking thymidine kinase activity or deficient in nucleoside transport were selected by growth in the presence of 5'-fluorodeoxyuridine and their sensitivity to interferon tested. All five thymidine kinase and both transport deficient mutants were sensitive to the antiproliferativ...

journal_title：Biochemical and biophysical research communications

authors： Gewert DR,Cohen A,Williams BR

更新日期：1984-01-13 00:00:00

abstract：:Vascular degeneration appears to play crucial roles in producing many vascular malfunctions (1-3). In order to identify specific inducers of programmed death in vascular endothelial cells (VEC), examinations were made of the effects of substances that are known to affect the vascular system by using VEC in culture (4,...

journal_title：Biochemical and biophysical research communications

authors： Araki S,Ishida T,Yamamoto T,Kaji K,Hayashi H

更新日期：1993-01-15 00:00:00

abstract：:2-keto-3-deoxy-6-phosphogluconate aldolase (E.C. 4.1.2.14) has been purified in two chromatographic steps to 99% purity in 73% overall yield from Azotobacter vinelandii. The pure enzyme is a 70 kD trimeric Class I aldolase, inhibitable by bromopyruvate or pyruvate plus sodium borohydride, with a specific activity of 6...

journal_title：Biochemical and biophysical research communications

authors： Taha TS,Deits TL

更新日期：1994-04-15 00:00:00

abstract：:The breakdown of blood clots requires the fibrinolytic action of the serine proteinase plasmin, a two-chain polypeptide derived posttranslationally from its precursor zymogen, plasminogen. While investigating plasminogen gene expression in human extrahepatic tissues, a cDNA sequence was obtained which closely resemble...

journal_title：Biochemical and biophysical research communications

authors： Weissbach L,Treadwell BV

更新日期：1992-07-31 00:00:00

abstract：:Polyclonal antibodies have been obtained against a synthetic dodecapeptide identical to the aminoacid sequence 120-131 DSPIKDGVWPPE (inferred from its DNA sequence) of Paracoccus denitrificans cytochrome c oxidase subunit III. The antibodies had a titer higher than 1:10000 when tested against the antigen. These antibo...

journal_title：Biochemical and biophysical research communications

authors： Müller M,Labonia N,Azzi A

更新日期：1988-08-15 00:00:00

abstract：:Cot/Tpl2, a member of MAP kinase kinase kinase (MAPKKK), is indispensable for the ERK activation, as well as the production of TNF-α, IL-1β, IL-23, and PGE(2) in lipopolysaccharide (LPS)-stimulated macrophages. However, the expression and the functional roles of Cot/Tpl2 in mast cells have not been elucidated. The adm...

journal_title：Biochemical and biophysical research communications

authors： Chiba N,Kakimoto K,Masuda A,Matsuguchi T

更新日期：2010-11-05 00:00:00

abstract：:Interaction of camel lens zeta-crystallin with aspirin was investigated by activity and fluorescence measurements. Aspirin minimally inhibited the oxidoreductase activity of the enzyme and weakly quenched its fluorescence. However, significant fluorescence quenching of zeta-crystallin coincided with the appearance of ...

journal_title：Biochemical and biophysical research communications

authors： Bazzi MD

更新日期：2002-04-26 00:00:00

abstract：:Seed germination is a crucial stage for plant development and agricultural production. To investigate its complex regulation process, the RNA-Seq study of rice embryo was conducted at three time points of 0, 12 and 48 h post imbibition (HPI). Dynamic transcriptional alterations were observed, especially in the early s...

journal_title：Biochemical and biophysical research communications

authors： Wei T,He Z,Tan X,Liu X,Yuan X,Luo Y,Hu S

更新日期：2015-08-14 00:00:00

abstract：:Dimerization-induced activation of LXR is a unique allosteric mechanism described only for LXR/RXR heterodimers. Previously, we demonstrated that RXR functions as an allosteric activator of LXR binding to ASC-2 coactivator rather than as a direct interaction partner. Here, we investigated the molecular basis of the in...

journal_title：Biochemical and biophysical research communications

authors： Son YL,Lee YC

更新日期：2009-07-03 00:00:00