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Evidence that post-transcriptional changes in the expression of mitogen regulated protein accompany immortalization of mouse cells.

Abstract：

:Primary mouse embryo fibroblasts passed in culture go through a period of declining growth rate and then after a period of minimal proliferation begin to grow again, generating, under appropriate conditions, an immortal 3T3 line. The 3T3 cells, but not the primary mouse embryo fibroblasts, are able to synthesize and to secrete mitogen regulated protein (MRP, also known as proliferin). We report here that the level at which the change in gene expression occurs during this spontaneous immortalization process is post-transcriptional, reflecting a change in either RNA processing or transcript stability. To our knowledge, this is the first report of an alteration at the post-transcriptional level of gene expression that accompanies immortalization.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Edwards DR,Parfett CL,Smith JH,Denhardt DT

doi

10.1016/s0006-291x(87)80144-4

subject

Has Abstract

pub_date

1987-08-31 00:00:00

pages

467-73

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(87)80144-4

journal_volume

147

pub_type

杂志文章

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