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The ferrous-oxy complex of human aromatase.

Abstract：

:In this communication, we document the self-assembly of heterologously expressed truncated human aromatase (CYP19) into nanometer scale phospholipids bilayers (Nanodiscs). The resulting P450 CYP19 preparation is stable and can tightly associate with the substrate androstenedione to form a nearly complete high-spin ferric protein. Ferrous CYP19 in Nanodiscs was mixed anaerobically in a rapid-scan stopped-flow with atmospheric dioxygen and the formation of the ferrous-oxy complex observed. First order decay of the oxy-complex to release superoxide and regenerate the ferric enzyme was monitored kinetically. Surprisingly, the ferrous-oxy complex of aromatase is more stable than that of hepatic CYP3A4, opening the path to precisely determine the biochemical and biophysical properties of the reaction cycle intermediates in this important human drug target.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Grinkova YV,Denisov IG,Waterman MR,Arase M,Kagawa N,Sligar SG

doi

10.1016/j.bbrc.2008.05.011

subject

Has Abstract

pub_date

2008-07-25 00:00:00

pages

379-82

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)00917-0

journal_volume

372

pub_type

杂志文章

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