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Radioimmunoassay for endothelin and immunoreactive endothelin in culture medium of bovine endothelial cells.

Abstract：

:Using a synthetic 21-residue endothelin as antigen, we have produced an antiserum for endothelin and developed a specific and sensitive radioimmunoassay (RIA) for endothelin. The minimum detection limit of the RIA was 1 pg/tube. Immunoreactive (ir-) endothelin was extracted from the culture medium by Bondelute C8 column. The ir-endothelin in the culture medium of endothelial cells (EC) from bovine pulmonary artery and carotid artery was 1.48 ng/ml and 3.31 ng/ml, respectively. Reverse-phase high performance liquid chromatography coupled with the RIA revealed that ir-endothelin in the culture medium comprised one major component corresponding to synthetic endothelin. In addition, the cultured EC of bovine pulmonary artery were specifically stained by immunohistochemical technique. These results suggest that endothelin could be produced in the EC of the pulmonary and carotid arteries besides the aorta. The RIA presented in this study could be an useful tool to investigate the pathophysiologic significance of endothelin.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Naruse M,Naruse K,Kurimoto F,Horiuchi J,Tsuchiya K,Kawana M,Kato Y,Zeng ZP,Sakurai H,Demura H

doi

10.1016/0006-291x(89)92484-4

subject

Has Abstract,Author List Incomplete

pub_date

1989-04-28 00:00:00

pages

662-8

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(89)92484-4

journal_volume

160

pub_type

杂志文章

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