Mitochondrial malate dehydrogenase and its precursor have different conformations.

Abstract:

:Antiserum prepared against the denatured form of mammalian malate dehydrogenase was found to immunoprecipitate the denatured but not the native form of the mature enzyme. In contrast, the antiserum immunoprecipitated the enzyme's precursor, synthesized in a rabbit reticulocyte lysate, either before or after denaturation. The mature form of the enzyme but not the precursor bound to an affinity column of 5'-AMP-Sepharose. These results indicate that the mature and precursor forms of malate dehydrogenase have different conformations.

authors

Chien SM,Freeman KB

doi

10.1016/s0006-291x(86)80370-9

subject

Has Abstract

pub_date

1986-11-26 00:00:00

pages

313-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(86)80370-9

journal_volume

141

pub_type

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