The role of lipopolysaccharide moieties in macrophage response to Escherichia coli.

abstract：:The neuronal presynaptic membrane t-SNARE complex consists of the transmembrane protein syntaxin with the palmitoylated protein SNAP-25. In non-neuronal tissues, SNAP-23 replaces SNAP-25 in the t-SNARE complex, although the mechanism of membrane anchoring of SNAP-23 has not been determined. We now report that like SNA...

journal_title：Biochemical and biophysical research communications

authors： Vogel K,Roche PA

更新日期：1999-05-10 00:00:00

abstract：:We show here that the susceptibility of endothelial cells to Shiga toxin (Stx)s differs remarkably depending on their cellular origins. The concentration of Stx-1 required to reduce cell viability by 50% as measured by MTT assay was 30 and 300 fM for neonatal and adult human microvascular endothelial cells (HMVEC), re...

journal_title：Biochemical and biophysical research communications

authors： Ohmi K,Kiyokawa N,Takeda T,Fujimoto J

更新日期：1998-10-09 00:00:00

abstract：:Hepatitis B, a major public health concern worldwide, is caused by hepatitis type B virus, a hepdnavirus that infects only human and certain nonhuman primates, and replicates strictly in hepatocytes. By using the techniques of slot and Southern blot DNA hybridization, and electron microscopy, the presence of HBV was i...

journal_title：Biochemical and biophysical research communications

authors： Pao CC,Wu SY,Hung IJ,Ng KT,Liaw YF,Lo SJ

更新日期：1987-07-31 00:00:00

abstract：:Anchoring protein alphaKAP targets calmodulin kinase II (CaMKII) to the sarcoplasmic reticulum (SR), and in the rabbit is a substrate of CaMKII itself in fast-twitch, but not in slow-twitch muscle. This work was aimed at elucidating the molecular basis for differential phosphorylation of alphaKAP. Here we show that tw...

journal_title：Biochemical and biophysical research communications

authors： Damiani E,Sacchetto R,Salviati L,Margreth A

更新日期：2003-02-28 00:00:00

abstract：:Kinetin (Kn) is a synthetic cytokinin plant growth hormone having some senescence-retarding effects in plants. Its effects on animal cells have been, until now, best considered as growth inhibitory and anti-tumorigenic. However, we have observed that an addition of 40-200 microM Kn in the culture medium of human diplo...

journal_title：Biochemical and biophysical research communications

authors： Rattan SI,Clark BF

更新日期：1994-06-15 00:00:00

abstract：:We investigated the mechanisms by which the heat shock response inhibits inducible nitric oxide synthase (iNOS) gene expression. Incubation of cultured murine lung epithelium (MLE-15) at temperatures ranging from 39 to 43 degrees C, for 1 h, demonstrated that only severe thermal stress (41 to 43 degrees C) was suffici...

journal_title：Biochemical and biophysical research communications

authors： Wong HR,Ryan M,Wispé JR

更新日期：1997-02-13 00:00:00

abstract：:Immunoglobulin A (IgA) nephropathy is an important cause of end-stage kidney disease (ESKD). Tubulointerstitial inflammation and subsequent fibrosis appear to be a major contributor of the disease progression to ESKD; however, the underlying mechanism is poorly understood. Herein, we report that a unique feature of CY...

journal_title：Biochemical and biophysical research communications

authors： Cui TG,Ichikawa T,Yang M,Dong X,Li J,Cui T

更新日期：2009-12-11 00:00:00

abstract：:Various bacteria and bacterial products induce in pure, lymphocyte-free bone marrow-derived mononuclear phagocytes (BMMø) the generation of tumor necrosis factor, nitric oxide (NO) synthase, NO and nitrite (NO2-), the flow of L-arginine to citrulline, and tumoricidal activity. The flow of L-arginine to citrulline and ...

journal_title：Biochemical and biophysical research communications

authors： Keller R,Bassetti S,Keist R,Mülsch A,Klauser S

更新日期：1992-05-15 00:00:00

abstract：:Measurement and validation of microarray gene signatures in routine clinical samples is problematic and a rate limiting step in translational research. In order to facilitate measurement of microarray identified gene signatures in routine clinical tissue a novel method combining quantum dot based oligonucleotide in si...

journal_title：Biochemical and biophysical research communications

authors： Tholouli E,MacDermott S,Hoyland J,Yin JL,Byers R

更新日期：2012-08-24 00:00:00

abstract：:Fractionation of rat uterine cells incubated at 22 degrees C with 0.2 nM [3H]-estradiol-17 beta (E2 beta) was performed to analyze the subcellular distribution of internalized hormone. The postnuclear supernatant of homogenates was resolved in Percoll density gradients into six major fractions defined by enzyme marker...

journal_title：Biochemical and biophysical research communications

authors： Pietras RJ,Szego CM

更新日期：1984-08-30 00:00:00

abstract：:Tendon calcification has been widely regarded by researchers to result from the osteogenic differentiation of Tendon-Derived Stem Cells (TDSCs) and ectopic mineralization caused by the calcification of cellular matrix. Recent studies have revealed a correlation between the Mg(2+)/Ca(2+) balance and the degeneration or...

journal_title：Biochemical and biophysical research communications

authors： Yue J,Jin S,Li Y,Zhang L,Jiang W,Yang C,Du J

更新日期：2016-09-09 00:00:00

abstract：:Using a monospecific antibody to the major cytosolic glutathione-S-transferase of human liver, we have isolated a cDNA clone from a human liver cDNA expression vector library in lambda gt11. The clone cross-hybridizes with a rat liver ligandin (glutathione-S-transferase 1-2) cDNA probe. The clone has an insert of 1.25...

journal_title：Biochemical and biophysical research communications

authors： Butera L,Monnier JR,Campbell E,Bhargava MM

更新日期：1987-02-13 00:00:00

abstract：:Although antisense strategy has been at the center of interest in gene therapy, little is known about application of this strategy to cardiac diseases because of the lack of a suitable delivery method into the heart. Therefore, we compared the transfection efficiency of antisense oligodeoxynucleotides (ODN) using HVJ-...

journal_title：Biochemical and biophysical research communications

authors： Aoki M,Morishita R,Higaki J,Moriguchi A,Kida I,Hayashi S,Matsushita H,Kaneda Y,Ogihara T

更新日期：1997-02-24 00:00:00

abstract：:Insect development is controlled by the combined actions of ecdysteroid and juvenile hormones. Transcriptional control by ecdysteroid hormones is mediated via two nuclear receptor superfamily members, ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle (USP). Although the ecdysteroid hormone 20-hydrox...

journal_title：Biochemical and biophysical research communications

authors： Maki A,Sawatsubashi S,Ito S,Shirode Y,Suzuki E,Zhao Y,Yamagata K,Kouzmenko A,Takeyama K,Kato S

更新日期：2004-07-16 00:00:00

abstract：:In the early postnatal period, cerebellar granule cells exhibit an activity-dependent downregulation of a set of immaturation genes involved in cell growth and migration and are shifted to establishment of a mature network formation. Through the use of a granule cell culture and both pharmacological and RNA interferen...

journal_title：Biochemical and biophysical research communications

authors： Okazawa M,Abe H,Nakanishi S

更新日期：2016-05-13 00:00:00

abstract：:HMG-CoA reductase inhibitor statins are used to treat patients with hypercholesterolemia. The pleiotropic effects of statins have been recently extended to the regulation of angiogenesis. However, the observations on the effects of statins on endothelial cells seem to be contradictory. In this work, we systematically ...

journal_title：Biochemical and biophysical research communications

authors： Hu K,Wan Q

更新日期：2019-04-02 00:00:00

abstract：:The oyster vasa-like gene was previously demonstrated to be specifically expressed in germline cells of adult oysters Crassostrea gigas. In the present study, this gene was used as a molecular marker to establish the developmental pattern of germline cells during oyster ontogenesis, using whole-mount in situ hybridiza...

journal_title：Biochemical and biophysical research communications

authors： Fabioux C,Huvet A,Lelong C,Robert R,Pouvreau S,Daniel JY,Minguant C,Le Pennec M

更新日期：2004-07-23 00:00:00

abstract：:Down syndrome (DS) leads to cardiac defects which are common and significant in babies with DS. We recently generated chimeric mice carrying a human chromosome (hChr) 21. The contribution ratio of embryonic stem (ES) cells containing a hChr 21 was specifically low in the heart, compared to other organs, and cardiovasc...

journal_title：Biochemical and biophysical research communications

authors： Inoue T,Shinohara T,Takehara S,Inoue J,Kamino H,Kugoh H,Oshimura M

更新日期：2000-06-24 00:00:00

abstract：:The kinetic parameters of several substrates of penicillin acylase from Streptomyces lavendulae have been determined. The enzyme hydrolyses phenoxymethyl penicillin (penicillin V) and other penicillins with aliphatic acyl-chains such as penicillin F, dihydroF, and K. The best substrate was penicillin K (octanoyl penic...

journal_title：Biochemical and biophysical research communications

authors： Torres-Guzmán R,de la Mata I,Torres-Bacete J,Arroyo M,Castillón MP,Acebal C

更新日期：2002-03-01 00:00:00

abstract：:Vomeronasal organs of female Wistar rats were exposed with sprayed urine preparations of male Wistar rats prior to sacrifice. Exposure to crude urine and ultrafiltrated urine preparation (<5000 Da) induced significant Fos expression, which is correlated with cellular activity, in the mitral/tufted cell layer of the ac...

journal_title：Biochemical and biophysical research communications

authors： Tsujikawa K,Kashiwayanagi M

更新日期：1999-06-24 00:00:00

abstract：:We have previously reported the establishment of preadipocyte cell lines, termed dedifferentiated fat (DFAT) cells, from mature adipocytes of various animals. DFAT cells possess long-term viability and can redifferentiate into adipocytes both in vivo and in vitro. Furthermore, DFAT cells can transdifferentiate into os...

journal_title：Biochemical and biophysical research communications

authors： Kazama T,Fujie M,Endo T,Kano K

更新日期：2008-12-19 00:00:00

abstract：:N-Acetylglucosaminyltransferase I (GlcNAcT-I, EC 2.4.1.101) is the enzyme which initiates the formation of complex N-linked glycans in eukaryotes by transforming GlcNAc to the oligo-mannosyl acceptor Man(5)GlcNAc(2)-Asn. The enzymatic activity and the structure that is synthesised by this enzyme are found in animals a...

journal_title：Biochemical and biophysical research communications

authors： Bakker H,Lommen A,Jordi W,Stiekema W,Bosch D

更新日期：1999-08-11 00:00:00

abstract：:The beta-subunit of human chorionic gonadotropin (beta-hCG) is secreted by many kinds of tumors and it has been used as an ideal target antigen to develop vaccines against tumors. In view of the low immunogenicity of this self-peptide,we designed a method based on isocaudamer technique to repeat tandemly the 10-residu...

journal_title：Biochemical and biophysical research communications

authors： Yankai Z,Rong Y,Yi H,Wentao L,Rongyue C,Ming Y,Taiming L,Jingjing L,Jie W

更新日期：2006-07-14 00:00:00

abstract：:Force impulse is thought to be generated in muscle when myosin head (S-1), while weakly bound to actin filament, undergoes orientational change to form a strong (rigor) bond with actin. There is ample evidence that this bond involves interaction of 1 myosin head with 1 actin monomer. However, X-ray diffraction data of...

journal_title：Biochemical and biophysical research communications

authors： Andreev OA,Borejdo J

更新日期：1991-05-31 00:00:00

abstract：:The role of osteoblasts in inducing the proliferation and differentiation of bone marrow cells was examined. Conditioned medium obtained from mouse osteoblastic cell (MC3T3-E1) cultures stimulated colony formation of mouse bone marrow cells (CSF) and differentiation of mouse myeloid leukemia cells (M1) into macrophage...

journal_title：Biochemical and biophysical research communications

authors： Shiina-Ishimi Y,Abe E,Tanaka H,Suda T

更新日期：1986-01-14 00:00:00

abstract：:ATP is the universal energy currency but mysteriously its cellular concentration is much higher than that needed for providing energy. Recently ATP was decoded to act as a hydrotrope to dissolve liquid-liquid phase separation (LLPS) of FUS whose aggregation leads to ALS/FTD. By DIC microscopy and NMR, here we characte...

journal_title：Biochemical and biophysical research communications

authors： Kang J,Lim L,Song J

更新日期：2018-10-02 00:00:00

abstract：:Phosphofructokinase (PFK) from human polymorphonuclear leukocytes (PMN) was characterized by immunological titration with subunit specific antibodies, column chromatography on QAE-Sephadex and SDS-polyacrylamide gel electrophoresis. Two different isozymes, M-type and L-type, were found. The M(r) values of the M and L ...

journal_title：Biochemical and biophysical research communications

authors： Durante P,Raleigh X,Gómez ME,Campos G,Ryder E

更新日期：1995-11-22 00:00:00

abstract：:In the course of the eukaryotic ribosomal biogenesis, both the nuclear import and export are involved. We have studied the nuclear and nucleolar localization of the human ribosomal protein S7. We examined the subcellular distribution of the S7:beta-galactosidase fusion protein in SAOS-2 cells. We have identified two e...

journal_title：Biochemical and biophysical research communications

authors： Annilo T,Karis A,Hoth S,Rikk T,Kruppa J,Metspalu A

更新日期：1998-08-28 00:00:00

abstract：:Amyotrophic lateral sclerosis (ALS) is a fatal motor neuron disease, whose primary mechanisms or causes are still not defined and for which no effective treatment is available. We have recently reported that before disease onset the level of tyrosine nitrated proteins is increased in the G93A SOD1 transgenic mouse mod...

journal_title：Biochemical and biophysical research communications

authors： Massignan T,Casoni F,Basso M,Stefanazzi P,Biasini E,Tortarolo M,Salmona M,Gianazza E,Bendotti C,Bonetto V

更新日期：2007-02-16 00:00:00

abstract：:Leber's hereditary optic neuropathy (LHON) has been associated with "primary" and "secondary" mtDNA missense point mutations, and a synergistic role has been proposed for secondary mutations. No previous study has investigated the effects of LHON primary or primary plus secondary mutations on the respiratory competenc...

journal_title：Biochemical and biophysical research communications

authors： Vergani L,Martinuzzi A,Carelli V,Cortelli P,Montagna P,Schievano G,Carrozzo R,Angelini C,Lugaresi E

更新日期：1995-05-25 00:00:00