Abstract：
:Phosphofructokinase (PFK) from human polymorphonuclear leukocytes (PMN) was characterized by immunological titration with subunit specific antibodies, column chromatography on QAE-Sephadex and SDS-polyacrylamide gel electrophoresis. Two different isozymes, M-type and L-type, were found. The M(r) values of the M and L subunits were 79,500 +/- 1,914 and 74,250 +/- 1,258, respectively. The two isozymes presented different kinetic and regulatory properties. The results suggest that PFK from human normal PMN is a mixture of M-type and L-type homotetramers, mainly, with possible minor heterotetrameric forms.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Durante P,Raleigh X,Gómez ME,Campos G,Ryder Edoi
10.1006/bbrc.1995.2706subject
Has Abstractpub_date
1995-11-22 00:00:00pages
898-905issue
3eissn
0006-291Xissn
1090-2104pii
S0006291X85727064journal_volume
216pub_type
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.05.059
更新日期：2006-07-14 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/0006-291x(91)91459-p
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：1988-04-15 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2008.08.016
更新日期：2008-10-17 00:00:00
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journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
doi：10.1016/j.bbrc.2006.04.012
更新日期：2006-06-16 00:00:00
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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doi：10.1006/bbrc.1995.1399
更新日期：1995-03-17 00:00:00
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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journal_title：Biochemical and biophysical research communications
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doi：10.1006/bbrc.1994.1987
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journal_title：Biochemical and biophysical research communications
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doi：10.1006/bbrc.2000.3090
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abstract：:Despite numerous studies on detergent-induced solubilization of membranes and on the underlying mechanisms associated with this process, very little is known regarding the selectivity of detergents for lipids during their extraction from membranes. To get insights about this phenomenon, solubilization of model bilayer...
journal_title：Biochemical and biophysical research communications
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abstract：:The human ST2 gene has been known to encode three splice variants; namely, a soluble secreted form of ST2, a transmembrane form of ST2L, and ST2V of undetermined localization. Therefore, analysis of tissue distribution and subcellular localization of ST2V is important to elucidate functional relationships among the th...
journal_title：Biochemical and biophysical research communications
pub_type： 杂志文章
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更新日期：2001-08-03 00:00:00
