当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Inhibitory and enhancing effects of insertion of central polypurine tract sequence on gene expression with vectors derived from human immunodeficiency virus type 1.

Inhibitory and enhancing effects of insertion of central polypurine tract sequence on gene expression with vectors derived from human immunodeficiency virus type 1.

Abstract：

:Reportedly, in human immunodeficiency virus type 1 (HIV) vectors, insertion of central polypurine tract (cPPT) increased expression of transgenes for a short period. To test this for a stable condition, we constructed a series of vectors carrying a Neo(r) gene as a stable marker driven by a synthetic thymidine kinase (hTK) promoter. Transduction efficiency was increased in about 2-fold and decreased in about 8-fold by insertion of the reported 178bp and our 282bp cPPTs, respectively. PCR analyses revealed that insertion of 282bp cPPT, but not 178bp cPPT, impaired integration, although it did not deteriorate nuclear transport much. Furthermore, we found that insertion of 282bp cPPT between hTK promoter and an upstream LTR sequence reduced reporter gene activity in about 5-fold. This inhibitory effect of 282bp cPPT may partly account for the observed decrease in transduction efficiency. We suggest that actual effect of cPPT insertion should be examined in each HIV vector.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Sakuma R,Kobayashi N,Ae K,Kitamura Y

doi

10.1016/s0006-291x(03)00212-2

subject

Has Abstract

pub_date

2003-03-14 00:00:00

pages

489-95

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X03002122

journal_volume

302

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • 5-Fluorouracil induces apoptosis through the suppression of NF-kappaB activity in human salivary gland cancer cells.

    abstract：:Activation of the transcription factor NF-kappaB results in protection against apoptosis, and the chemotherapeutic agent 5-Fluorouracil (5-FU) exerts its cytotoxic effect through the induction of apoptosis. Thus, we examined whether 5-FU could induce apoptosis through the suppression of NF-kappaB activity. We found th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3072

    authors： Aota K,Azuma M,Yamashita T,Tamatani T,Motegi K,Ishimaru N,Hayashi Y,Sato M

    更新日期：2000-07-14 00:00:00

  • Membrane topology of human AGPAT3 (LPAAT3).

    abstract：:Integral membrane lysophospholipid acyltransferases (AT) are involved in many reactions that produce phospholipids and triglycerides. Enzymes that utilize lysophosphatidic acid (LPA) as an acceptor substrate have been termed LPAATs, and several are members of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) ge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.05.149

    authors： Schmidt JA,Yvone GM,Brown WJ

    更新日期：2010-07-09 00:00:00

  • Genome-wide screening by cDNA microarray of genes associated with matrix mineralization by human mesenchymal stem cells in vitro.

    abstract：:Using a culture system that facilitates osteogenic differentiation of bone marrow-derived human mesenchymal stem cells, we analyzed gene-expression profiles during the mineralization process by means of a cDNA microarray system consisting of 23,040 genes. We compared expression profiles of the cells at days 3, 15, and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6196

    authors： Doi M,Nagano A,Nakamura Y

    更新日期：2002-01-11 00:00:00

  • Isolation and characterization of iso-rANP, a new natriuretic peptide from rat atria.

    abstract：:Using a specific radioimmunoassay we have isolated and sequenced a new 45-amino acid peptide from rat atria which exhibits similar physiological and pharmacological properties to rat atrial natriuretic peptide (rANP). We have termed the new peptide iso-rANP, because of its functional and structural similarities to rAN...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92675-2

    authors： Flynn TG,Brar A,Tremblay L,Sarda I,Lyons C,Jennings DB

    更新日期：1989-06-15 00:00:00

  • PPARgamma activation abolishes LDL-induced proliferation of human aortic smooth muscle cells via SOD-mediated down-regulation of superoxide.

    abstract：:Native LDL would be a mitogenic and chemotactic stimulus of VSMC proliferation and differentiation in the atherosclerotic lesion where endothelial disruption occurred. In previous studies, our group investigated the molecular mechanisms by which LDL induces IL-8 production and by which PPARalpha activation abolishes L...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.006

    authors： Heo KS,Kim DU,Ryoo S,Nam M,Baek ST,Kim L,Park SK,Myung CS,Hoe KL

    更新日期：2007-08-10 00:00:00

  • Mdm2 is a novel activator of ApoCIII promoter which is antagonized by p53 and SHP inhibition.

    abstract：:We examined the effect of Mdm2 on regulation of the ApoCIII promoter and its cross-talk with p53 and nuclear receptor SHP. Overexpression of Mdm2 markedly enhanced ApoCIII promoter activity by HNF4α. A direct association of Mdm2 protein with the HNF4α protein was observed by co-immunoprecipitation. Ectopic expression ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.027

    authors： Yang Z,Zhang Y,Wang L

    更新日期：2012-01-13 00:00:00

  • Application of the ferromagnetic transduction model to D.C. and pulsed magnetic fields: effects on epileptogenic tissue and implications for cellular phone safety.

    abstract：:The ferromagnetic transduction model proposed by J.L. Kirschvink (Phys. Rev. A (1992) 46, 2178-2184) suggests that the coupling of biogenic magnetic particles in the human brain to mechanosensitive membrane ion gates may provide a mechanism for interactions of environmental magnetic fields with humans. Extremely low f...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1575

    authors： Dobson J,St Pierre T

    更新日期：1996-10-23 00:00:00

  • Monocarboxylate transporters in breast cancer and adipose tissue are novel biomarkers and potential therapeutic targets.

    abstract：:Monocarboxylate transporters (MCTs) are transmembrane proteins that control the lactate metabolism and associated with poor prognosis in solid tumours including breast cancer (BC). This study aimed to evaluate the clinical and prognostic value of MCTs used by immunohistochemistry and quantum dots-based fluorescent ima...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.091

    authors： Li Z,Wu Q,Sun S,Wu J,Li J,Zhang Y,Wang C,Yuan J,Sun S

    更新日期：2018-07-02 00:00:00

  • Free calcium rise and mitogenesis in glial cells caused by endothelin.

    abstract：:The peptide endothelin causes a biphasic rise in intracellular free calcium levels in cultured Type-1 astrocytes and C6 glioma cells, suggesting that glial cells may be the physiological target of endothelin in the brain. Endothelin also causes a calcium-dependent increase [3H]thymidine incorporation in primary cultur...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92718-6

    authors： Supattapone S,Simpson AW,Ashley CC

    更新日期：1989-12-29 00:00:00

  • Phosphorylation of RhoGDI1 by p21-activated kinase 2 mediates basic fibroblast growth factor-stimulated neurite outgrowth in PC12 cells.

    abstract：:We previously showed that p21-activated kinase 2 (PAK2), a major PAK isoform expressed in PC12 cells, mediates neurite outgrowth via Rac1 GTPase. RhoGDI1 forms a complex with Rac1, resulting in its inhibition. Rac1 activation requires dissociation from RhoGDI1. Here, we show that PAK2 mediates basic fibroblast growth ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.12.066

    authors： Shin EY,Shim ES,Lee CS,Kim HK,Kim EG

    更新日期：2009-02-06 00:00:00

  • Mutations of phosphorylation sites Ser10 and Thr187 of p27Kip1 abolish cytoplasmic redistribution but do not abrogate G0/1 phase arrest in the HepG2 cell line.

    abstract：:The cyclin-dependent kinase (CDK) inhibitor p27(Kip1) is an important regulator of cell cycle progression as it negatively regulates G(0/1) progression and plays a major role in controlling the cell cycle. The screening of the p27(Kip1) sequence identified many potential phosphorylation sites. Although Ser(10) and Thr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.06.114

    authors： Guan X,Chen L,Wang J,Geng H,Chu X,Zhang Q,Du L,De W

    更新日期：2006-09-01 00:00:00

  • Nerve growth factor mRNA in brain: localization by in situ hybridization.

    abstract：:Nerve Growth Factor is a 118 amino acid polypeptide that plays an important role in the differentiation and survival of neurons. The recent discovery that a mRNA that encodes beta Nerve Growth Factor is present in brain suggests that the Nerve Growth Factor gene may not only regulate gene expression of peripheral but ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80569-1

    authors： Rennert PD,Heinrich G

    更新日期：1986-07-31 00:00:00

  • AKT-p53 axis protect cancer cells from autophagic cell death during nutrition deprivation.

    abstract：:An altered metabolism supports growth of tumor. AKT, a major signal integrator plays a key role in cell metabolism. We have shown that nutritional deprivation activates AKT as observed by increased phosphorylation of both Thr308 and Ser473. Pharmacological inhibition or silencing of AKT by siRNA affects cell viability...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.02.064

    authors： Sudhagar S,Sathya S,Gokulapriya G,Lakshmi BS

    更新日期：2016-03-18 00:00:00

  • Identification of two additional plasmodesmata localization domains in the tobacco mosaic virus cell-to-cell-movement protein.

    abstract：:Despite decades of intensive studies, the failure to identify plasmodesmata (PD) localization sequences has constrained our understanding of Tobacco mosaic virus (TMV) movement. Recently, we identified the first PD localization signal (major PLS) in the TMV movement protein (MP), which encompasses the first 50 amino a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.10.093

    authors： Liu Y,Huang C,Zeng J,Yu H,Li Y,Yuan C

    更新日期：2020-01-01 00:00:00

  • Inverse correlation between jasmonic acid and salicylic acid during early wound response in rice.

    abstract：:This study presents a kinetic analysis of the response to wounding in rice plants. In particular, jasmonic acid, salicylic acid, and lipoxygenase activity were measured in leaves of wounded rice plants during the early tillering phase. The results show that endogenous jasmonic acid transiently increases to a maximum 3...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.095

    authors： Lee A,Cho K,Jang S,Rakwal R,Iwahashi H,Agrawal GK,Shim J,Han O

    更新日期：2004-06-04 00:00:00

  • Validation of methods for CYP2C9 genotyping: frequencies of mutant alleles in a Swedish population.

    abstract：:Cytochrome P450 2C9 (CYP2C9) catalysis the metabolism of important drugs such as phenytoin, S-warfarin, tolbutamide, losartan, torasemide, and nonsteroidal anti-inflammatory drugs. A functional polymorphism of the CYP2C9 gene has been described. The variant alleles include CYP2C9*2 having a point mutation in exon 3 ca...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9992

    authors： Yasar U,Eliasson E,Dahl ML,Johansson I,Ingelman-Sundberg M,Sjöqvist F

    更新日期：1999-01-27 00:00:00

  • Comparison of repair of methylated pyrimidines in poly(dT) by extracts from rat liver and Escherichia coli.

    abstract：:Partially purified preparations of O6-alkylguanine-DNA alkyltransferase from rat liver and E. coli were tested for their ability to repair O4-methylthymine in a methylated poly(dT) X poly(dA) substrate. The bacterial preparation readily carried out this reaction, but no loss of O4-methylthymine was obtained with the r...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)90416-9

    authors： Dolan ME,Scicchitano D,Singer B,Pegg AE

    更新日期：1984-08-30 00:00:00

  • mTOR inhibition sensitizes human hepatocellular carcinoma cells to resminostat.

    abstract：:Histone deacetylases (HDACs) hyper-activity in hepatocellular carcinoma (HCC) is often associated with patients' poor prognosis. Our previous study has shown that resminostat, a novel HDAC inhibitor (HDACi), activated mitochondrial permeability transition pore (mPTP)-dependent apoptosis pathway in HCC cells. Here we e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.06.060

    authors： Peng X,Zhang D,Li Z,Fu M,Liu H

    更新日期：2016-09-02 00:00:00

  • Human monocytes possess a serine protease activity capable of degrading HIV-1 reverse transcriptase in vitro.

    abstract：:Human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) plays a central role in the virus replication cycle. We found that HIV-1 RT was rapidly degraded when incubated with cell extracts obtained from human peripheral blood cells. The proteolytic activity responsible for the in vitro degradation of RT w...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5252

    authors： Château MT,Robert-Hebmann V,Devaux C,Lazaro JB,Canard B,Coux O

    更新日期：2001-07-27 00:00:00

  • Real-time SPR characterization of the interactions between multi-epitope proteins and antibodies against classical swine fever virus.

    abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.12.104

    authors： Tian H,Hou X,Liu X

    更新日期：2013-02-08 00:00:00

  • Determining the 3D structure of human ASC2 protein involved in apoptosis and inflammation.

    abstract：:ASC2 structure has been well defined by 1141 NOE experimental restraints. The model consists of five alpha helices. alpha-Helices are connected by short random structure loops. The sole exception is the loop connecting helices 2 and 3, which has a 20-residue length. Folding generally agrees with the folding of recentl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.087

    authors： Espejo F,Patarroyo ME

    更新日期：2006-02-17 00:00:00

  • p21-activated kinase PAK phosphorylates desmin at sites different from those for Rho-associated kinase.

    abstract：:p21-activated kinase (PAK) and Rho-associated kinase (Rho-kinase) have been shown to induce Ca(2+)-independent contraction of smooth muscle. PAK-induced contraction of Triton-skinned smooth muscle correlates with increased phosphorylation of caldesmon and desmin, although the role of desmin phosphorylation has remaine...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2854

    authors： Ohtakara K,Inada H,Goto H,Taki W,Manser E,Lim L,Izawa I,Inagaki M

    更新日期：2000-06-16 00:00:00

  • Crystal structure of BrlR with c-di-GMP.

    abstract：:The transcriptional regulator BrlR is a member of the MerR family of multidrug transport activators in Pseudomonas aeruginosa. Recent study indicates that BrlR is a novel 3',5'-cyclic diguanylic acid (c-di-GMP) receptor and can be activated by c-di-GMP. To gain insight into BrlR function, we determined the structure o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.033

    authors： Raju H,Sharma R

    更新日期：2017-08-19 00:00:00

  • Cytohesin-associated scaffolding protein (CASP) is involved in migration and IFN-γ secretion in natural killer cells.

    abstract：:Natural Killer (NK) cells are highly mobile, specialized sub-populations of lymphocytic cells that survey their host to identify and eliminate infected or tumor cells. They are one of the key players in innate immunity and do not need prior activation through antigen recognition to deliver cytotoxic packages and relea...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.07.065

    authors： Tompkins N,MacKenzie B,Ward C,Salgado D,Leidal A,McCormick C,Pohajdak B

    更新日期：2014-08-22 00:00:00

  • Lack of nitric oxide synthase depresses ion transporting enzyme function in cardiac muscle.

    abstract：:Nitric oxide (NO*) is produced endogenously from NOS isoforms bound to sarcolemmal (SL) and sarcoplasmic reticulum (SR) membranes. To investigate whether locally generated NO* directly affects the activity of enzymes mediating ion active transport, we studied whether knockout of selected NOS isoforms would affect the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00599-5

    authors： Zhou L,Burnett AL,Huang PL,Becker LC,Kuppusamy P,Kass DA,Kevin Donahue J,Proud D,Sham JS,Dawson TM,Xu KY

    更新日期：2002-06-28 00:00:00

  • Reversal of P-glycoprotein-mediated MDR by 5,7,3',4',5'-pentamethoxyflavone and SAR.

    abstract：:During screening for the flavonoid chemosensitizers, it was found that 5,7,3',4',5'-pentamethoxyflavone (PMF) was equipotent to verapamil in vitro with respect to the chemosensitizing effect. PMF appears to have a chemosensitizing effect not only by increasing the intracellular accumulation of the drugs without compet...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.06.020

    authors： Choi CH,Kim JH,Kim SH

    更新日期：2004-07-30 00:00:00

  • Biological properties of purified recombinant HCV particles with an epitope-tagged envelope.

    abstract：:To establish a simple system for purification of recombinant infectious hepatitis C virus (HCV) particles, we designed a chimeric J6/JFH-1 virus with a FLAG (FL)-epitope-tagged sequence at the N-terminal region of the E2 hypervariable region-1 (HVR1) gene (J6/JFH-1/1FL). We found that introduction of an adaptive mutat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.081

    authors： Takahashi H,Akazawa D,Kato T,Date T,Shirakura M,Nakamura N,Mochizuki H,Tanaka-Kaneko K,Sata T,Tanaka Y,Mizokami M,Suzuki T,Wakita T

    更新日期：2010-05-14 00:00:00

  • Effects of osmotic stress and hyperglycemia on aldose reductase gene expression in human renal proximal tubule cells.

    abstract：:Sorbitol levels in proximal tubule cells cultured for 96 h in the presence of 16.5 mM and 27.5 mM glucose were significantly elevated when compared to cells cultured in 5.5 mM glucose. No changes in the levels of aldose reductase activity, mRNA and immunoreactivity were observed in cells cultured for up to 168 h in me...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81479-2

    authors： Petrash JM,Flath M,Sens D,Bylander J

    更新日期：1992-08-31 00:00:00

  • Identification of a novel Ca2+-stimulated S6-kinase in rat liver.

    abstract：:Extracellular calcium addition transiently stimulated two S6 peptide kinase activities in isolated rat hepatocytes. Mono Q chromatography revealed that the activities eluting at 0.15 M NaCl and 0.18 M NaCl were stimulated 4-fold and 2-fold, respectively. The kinase stimulated by calcium was a 40000-Mr S6 peptide kinas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7175

    authors： Harlin JC,Tredano M,Rider MH,Bollen M,Chédeville A,Lavoinne A

    更新日期：1997-10-20 00:00:00

  • Serum profiling based on fucosylated glycoproteins for differentiating between chronic hepatitis B and hepatocellular carcinoma.

    abstract：:Chronic infection with hepatitis B virus (HBV) is associated with the majority of cases of hepatocellular carcinoma (HCC) in China. Despite this, there is no effective method for the early detection of HBV-induced liver cancer. Aberrant fucosylation is known to occur during the development of HCC. We, therefore, devel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.02.155

    authors： Liao J,Zhang R,Qian H,Cao L,Zhang Y,Xu W,Li J,Wu M,Yin Z

    更新日期：2012-04-06 00:00:00