Lack of nitric oxide synthase depresses ion transporting enzyme function in cardiac muscle.

abstract：:We describe the fusion of enhanced green fluorescent protein to the C-terminus of the HsdS DNA sequence-specificity subunit of the Type I DNA modification methyltransferase M.EcoKI. The fusion expresses well in vivo and assembles with the two HsdM modification subunits. The fusion protein functions as a sequence-speci...

journal_title：Biochemical and biophysical research communications

authors： Chen K,Roberts GA,Stephanou AS,Cooper LP,White JH,Dryden DT

更新日期：2010-07-23 00:00:00

abstract：:Aurora-C, a member of the Aurora kinase family, is implicated in the regulation of mitosis. In contrast to Aurora-A and Aurora-B its cellular localization and functions are poorly characterized. TACC1 protein belongs to the transforming acidic coiled-coil family shown to interact with the Aurora kinases. In the presen...

journal_title：Biochemical and biophysical research communications

authors： Gabillard JC,Ulisse S,Baldini E,Sorrenti S,Cremet JY,Coccaro C,Prigent C,D'Armiento M,Arlot-Bonnemains Y

更新日期：2011-05-20 00:00:00

abstract：:As studied by intravital microscopy, local challenge with the mast cell secretagogue compound 48/80 was found to increase the leukocyte rolling fraction, decrease rolling velocity and induce firm leukocyte adhesion in postcapillary venules of the rat mesentery. These effects of compound 48/80 were inhibited by a monoc...

journal_title：Biochemical and biophysical research communications

authors： Thorlacius H,Raud J,Rosengren-Beezley S,Forrest MJ,Hedqvist P,Lindbom L

更新日期：1994-09-15 00:00:00

abstract：:Ferrous oxygenated (Fe(II)O2) hemoglobins (Hb's) and myoglobins (Mb's) have been shown to react very rapidly with NO, yielding NO3(-) and the ferric heme-protein derivative (Fe(III)), by means of the ferric heme-bound peroxynitrite intermediate (Fe(III)OONO), according to the minimum reaction scheme: Fe(II)O2 + NO (k(...

journal_title：Biochemical and biophysical research communications

authors： Ascenzi P,Bocedi A,Bolognesi M,Fabozzi G,Milani M,Visca P

更新日期：2006-01-06 00:00:00

abstract：BACKGROUND:Recently, it has been reported that hypoxia highly enhances expression of peptidylarginine deiminase (PAD) 4 and production of citrullinated proteins in some tumor cells. However, little is known about malignant gliomas on this issue. Therefore, we here investigated whether expression of PADs was induced by ...

journal_title：Biochemical and biophysical research communications

authors： Sase T,Arito M,Onodera H,Omoteyama K,Kurokawa MS,Kagami Y,Ishigami A,Tanaka Y,Kato T

更新日期：2017-01-01 00:00:00

abstract：:KCNQ1 plays important roles in the cardiac action potential and consists of an N-terminal domain, a voltage-sensor domain, a pore domain and a C-terminal domain. KCNQ1 is a voltage-gated potassium channel and its channel activity is regulated by membrane potentials. The linker between transmembrane helices 4 and 5 (S4...

journal_title：Biochemical and biophysical research communications

authors： Gayen S,Li Q,Kang C

更新日期：2015-01-02 00:00:00

abstract：:In order to search for novel estrogen-responsive genes, we performed serial analysis of gene expression (SAGE) for estrogen-treated MCF-7 human breast cancer cells. SAGE analysis of 31,000 and 30,856 tags from non-treated and 17 beta-estradiol (E2)-treated cells for 24 h, respectively, facilitated the identification o...

journal_title：Biochemical and biophysical research communications

authors： Inadera H,Hashimoto S,Dong HY,Suzuki T,Nagai S,Yamashita T,Toyoda N,Matsushima K

更新日期：2000-08-18 00:00:00

abstract：:We present an easy and sensitive method for measuring thiamine and its phosphate esters in small biological samples of microalgae (Amphidinium carterae Hulburt and Nitzschia microcephala Grun). The method consists of extraction of thiamine and its derivatives in acid solution, followed by liquid chromatography with fl...

journal_title：Biochemical and biophysical research communications

authors： Pinto E,Pedersén M,Snoeijs P,Van Nieuwerburgh L,Colepicolo P

更新日期：2002-02-22 00:00:00

abstract：:Insulin-like growth factor binding protein (IGFBP) gene expression and IGFBP secretion were investigated in a retinoic acid (RA)-resistant subline (RROI) of MCF-7 human breast carcinoma cells. Our results demonstrate that RRO-I cells constitutively secrete higher levels of IGFBP-3 and IGFBP-5. In addition, we found th...

journal_title：Biochemical and biophysical research communications

authors： Sheikh MS,Shao ZM,Chen JC,Clemmons DR,Roberts CT Jr,LeRoith D,Fontana JA

更新日期：1992-11-16 00:00:00

abstract：:The carboxyl-terminal domain of the zeta 1 subunit of the mouse NMDA receptor channel produced as a fusion protein with GST was phosphorylated in vitro by PKC. A mutant of the zeta 1 subunit without serine or threonine residues in the carboxyl-terminal domain (zeta 1-2-NST) was constructed and was expressed alone or t...

journal_title：Biochemical and biophysical research communications

authors： Yamakura T,Mori H,Shimoji K,Mishina M

更新日期：1993-11-15 00:00:00

abstract：:Transforming growth factor alpha (TGF alpha) induces dose- and time-dependent stimulation of prostacyclin (PGI2) production by cultured human umbilical vein endothelial cells. The lowest stimulatory concentration of TGF alpha was 0.1 ng/ml and the maximal response, a 2.7-fold rise, was obtained with 10 ng/ml. The stim...

journal_title：Biochemical and biophysical research communications

authors： Ristimäki A

更新日期：1989-05-15 00:00:00

abstract：:The effect of subchronic intracerebroventricular (i.c.v.) injection of the human immunodeficiency virus type 1 (HIV-1) recombinant protein gp120 (100 ng, given daily for up to 7 consecutive days) on cyclooxygenase type 2 (COX-2) expression was studied by immunohistochemistry in the brain of adult rats. In comparison t...

journal_title：Biochemical and biophysical research communications

authors： Bagetta G,Corasaniti MT,Paoletti AM,Berliocchi L,Nisticò R,Giammarioli AM,Malorni W,Finazzi-Agrò A

更新日期：1998-03-27 00:00:00

abstract：:Isolated P. chabaudi parasites were permeabilized with digitonin and the function of intracellular Ca2+ stores was studied using the Ca2+ indicators arsenazo III or Fluo 3-acid in the medium. Addition of the second messenger InsP3 (5 microM) to permeabilized parasites leads to Ca2+ release into the medium, with the me...

journal_title：Biochemical and biophysical research communications

authors： Passos AP,Garcia CR

更新日期：1998-04-07 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) is produced by various normal and neoplastic tissues. Even if the physiological function(s) of PTHrP is unclear, evidence suggests that the protein may participate in the local regulation of smooth muscle contractility. We show here that PTHrP is produced in endothelial cell...

journal_title：Biochemical and biophysical research communications

authors： Rian E,Jemtland R,Olstad OK,Endresen MJ,Grasser WA,Thiede MA,Henriksen T,Bucht E,Gautvik KM

更新日期：1994-01-28 00:00:00

abstract：:We have discovered two new exons in the mouse fibroblast growth factor 2 (FGF-2 or bFGF) gene that can be alternatively spliced to the second coding exon of the gene. The newly identified exons 1b and 1c are located at, respectively, approximately 19 and 32 kb downstream of the canonical exon 1a. Using RT-PCR analysis...

journal_title：Biochemical and biophysical research communications

authors： Dirks RP,Potter SJ,Griep AE

更新日期：2001-10-05 00:00:00

abstract：:S-modulin is a 26 kDa protein that regulates light sensitivity of cGMP phosphodiesterase in a Ca(2+)-dependent manner in frog rod outer segments (ROSs). In the present study, we purified S-modulin by taking advantage of a hydrophobic interaction between Phenyl Sepharose and S-modulin at high Ca2+ concentrations. The y...

journal_title：Biochemical and biophysical research communications

authors： Kawamura S,Takamatsu K,Kitamura K

更新日期：1992-07-15 00:00:00

abstract：:The major form of cytochrome P-450, P-450K-5, was purified from kidney microsomes of untreated male rats with high-performance liquid chromatography with anion-exchange and hydroxylapatite columns. The monomeric molecular weight of P-450K-5 was 52000 on SDS-polyacrylamide gel electrophoresis and the CO-reduced absorpt...

journal_title：Biochemical and biophysical research communications

authors： Imaoka S,Funae Y

更新日期：1986-12-15 00:00:00

abstract：:The preferred dye binding sites and the microenvironment of known nucleotide sequences within mitochondrial and plasmid pBR322 DNA was probed in a gross fashion with restriction endonucleases. The intercalating dyes, ethidium bromide and propidium iodide, do not inhibit a given restriction endonuclease equally at all ...

journal_title：Biochemical and biophysical research communications

authors： Soslau G,Pirollo K

更新日期：1983-09-15 00:00:00

abstract：:The three genes that form the UGA regulon in Saccharomyces cerevisiae are responsible for the transport and degradation of γ-aminobutyric acid (GABA) in this organism. Despite the differences in the sequence of their promoters, these genes similarly respond to GABA stimuli. The expression of UGA1, UGA2 and UGA4 depend...

journal_title：Biochemical and biophysical research communications

authors： Cardillo SB,Correa García S,Bermúdez Moretti M

更新日期：2011-07-15 00:00:00

abstract：:MDP, a synthetic muramyl dipeptide, is capable of increasing the primary in vitro antibody response, to sheep erythrocytes, of purified B cells restored with a monokine and helper T cell factors, including Interleukin 2 and the late-acting T cell replacing factor (TRF). First, the possibility that the adjuvanticity of...

journal_title：Biochemical and biophysical research communications

authors： Souvannavong V,Adam A

更新日期：1984-11-30 00:00:00

abstract：:In epithelial cells progression through the G1 phase of the cell cycle and preparing the cell for the S phase is regulated by cyclin D1-cdk4. Cells that express the retinoblastoma protein (pRb) are dependent on cyclin D1-cdk4 activity for their proliferation while cells that do not express pRb are not. Overexpression ...

journal_title：Biochemical and biophysical research communications

authors： Kent LL,Hull-Campbell NE,Lau T,Wu JC,Thompson SA,Nori M

更新日期：1999-07-14 00:00:00

abstract：:alpha-Crystallin, a major protein of the lens, is known to have chaperone activity to protect other proteins against thermal aggregation. Heat-induced structural change of alpha-crystallin was previously shown to increase its chaperone activity. In this report, we studied the thermal reversibility of alpha-crystallin ...

journal_title：Biochemical and biophysical research communications

authors： Lee JS,Satoh T,Shinoda H,Samejima T,Wu SH,Chiou SH

更新日期：1997-08-18 00:00:00

abstract：:The ability to identify apoptotic cells within a complex population is crucial in the research and diagnosis of normal physiology and disease states. The Cellscan mark S (CS-S) cytometer was used in this study to detect intracellular fluorescence intensity and polarization (FI and FP) in several well-established model...

journal_title：Biochemical and biophysical research communications

authors： Zurgil N,Schiffer Z,Shafran Y,Kaufman M,Deutsch M

更新日期：2000-02-05 00:00:00

abstract：:Cytoplasmic dyneins are multisubunit minus-end-directed microtubule motors. Different isoforms of dynein are thought to provide a means for independent movement of different organelles. We investigated the differential regulation of dynein-driven transport of pigment organelles (melanosomes) in Xenopus melanophores. A...

journal_title：Biochemical and biophysical research communications

authors： Reilein AR,Serpinskaya AS,Karcher RL,Dujardin DL,Vallee RB,Gelfand VI

更新日期：2003-09-26 00:00:00

abstract：:Slingshot-1 (SSH1) is a protein phosphatase that specifically dephosphorylates and activates cofilin, an F-actin-severing protein. SSH1 binds to and co-localizes with F-actin, and the cofilin-phosphatase activity of SSH1 is markedly increased by binding to F-actin. In this study, we performed a secondary structure ana...

journal_title：Biochemical and biophysical research communications

authors： Takahashi K,Okabe H,Kanno SI,Nagai T,Mizuno K

更新日期：2017-01-22 00:00:00

abstract：:It is still unclear whether an active state of transcription once established in chromatin persists in neurons. Here, we focused on BDNF exon I-IX mRNA expression because of its marked induction upon the treatment of rat cortical neurons with trichostatin A, suggesting strong repression of the expression through histo...

journal_title：Biochemical and biophysical research communications

authors： Hara D,Miyashita T,Fukuchi M,Suzuki H,Azuma Y,Tabuchi A,Tsuda M

更新日期：2009-12-18 00:00:00

abstract：:This paper reports the purification of three myotropic neuropeptides from the white shrimp Penaeus vannamei. The central nervous systems of 3500 shrimps were extracted in an acidified solvent, after which four to five HPLC column systems were used to obtain pure peptides. A cockroach hindgut muscle contraction bioassa...

journal_title：Biochemical and biophysical research communications

authors： Nieto J,Veelaert D,Derua R,Waelkens E,Cerstiaens A,Coast G,Devreese B,Van Beeumen J,Calderon J,De Loof A,Schoofs L

更新日期：1998-07-20 00:00:00

abstract：:Neurodegeneration causes dysfunction and degeneration of neurons and is triggered by various factors including genetic defects, free radicals, injury, and glutamate excitotoxicity. Among those, glutamate excitotoxicity is implicated in chronic disorders including AD and ALS, and in acute insults in the CNS including t...

journal_title：Biochemical and biophysical research communications

authors： Fujiwara T,Morimoto K

更新日期：2013-10-25 00:00:00

abstract：:Five different receptor subtypes for neuropeptide Y (NPY) have recently been cloned in mammals. We have discovered three distinct subtypes by PCR in the zebrafish, Danio rerio, and describe here one of these called zYc. The protein sequence identity is 46-51% to mammalian subtypes Y1, Y4 and Y6 and to zebrafish Ya, i....

journal_title：Biochemical and biophysical research communications

authors： Ringvall M,Berglund MM,Larhammar D

更新日期：1997-12-29 00:00:00

abstract：:Angiotensin II (Ang II) modulates VSMCs phenotypic switch that play a critical role in the cardiovascular diseases. MicroRNA-27a (miR-27a) has been proven to be involved in regulating vascular remodeling; however, the functional role of miR-27a in VSMCs in response to Ang II stimulation need to be elucidated. Cell pro...

journal_title：Biochemical and biophysical research communications

authors： Xu MM,Deng HY,Li HH

更新日期：2019-02-19 00:00:00