Abstract:
:alpha-Crystallin, a major protein of the lens, is known to have chaperone activity to protect other proteins against thermal aggregation. Heat-induced structural change of alpha-crystallin was previously shown to increase its chaperone activity. In this report, we studied the thermal reversibility of alpha-crystallin and the effect of change in secondary structure on its chaperone function in vitro. The heat-induced conformational changes in the aromatic region of near-UV CD spectra showed only a small degree of reversibility. The structural transitions from 50 to 70 degrees C were largely reversible if the incubation time was short. However, the protective ability to inhibit thermal aggregation of alcohol dehydrogenase by alpha-crystallin was essentially similar at 48 and 70 degrees C. Under long-term heating at high temperatures, there was a time-dependent irreversibility of structural change in alpha-crystallin as revealed by CD spectroscopy. Such denatured alpha-crystallin by long-term heating can still preserve its ability to prevent UV-induced aggregation of gamma-crystallin at room temperature, indicating relatively little effect of heat-induced changes in secondary structure on the chaperone activity of alpha-crystallin.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Lee JS,Satoh T,Shinoda H,Samejima T,Wu SH,Chiou SHdoi
10.1006/bbrc.1997.7131subject
Has Abstractpub_date
1997-08-18 00:00:00pages
277-82issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(97)97131-Xjournal_volume
237pub_type
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