当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Fluorescein fluorescence hyperpolarization as an early kinetic measure of the apoptotic process.

Fluorescein fluorescence hyperpolarization as an early kinetic measure of the apoptotic process.

Abstract：

:The ability to identify apoptotic cells within a complex population is crucial in the research and diagnosis of normal physiology and disease states. The Cellscan mark S (CS-S) cytometer was used in this study to detect intracellular fluorescence intensity and polarization (FI and FP) in several well-established models of apoptosis: Following spontaneous apoptosis, as well as glucocorticoid or anti Fas-induced apoptosis, CS-S individual cell-based analysis revealed the appearance of a cell cluster characterized by low FI and high FP. Temporal analysis of annexine V binding and FP measurements following DXM treatment showed that hyperpolarization preceded phosphatidylserine appearance on the outer plasma membrane. The early increase in FP was found to be dose dependent and inversely related to cell diameter. Cell dehydration and alteration of plasma membrane transport properties, both occurring during early stages of apoptosis, may be involved in the phenomena of intracellular fluorescein hyper-polarization in apoptosis.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Zurgil N,Schiffer Z,Shafran Y,Kaufman M,Deutsch M

doi

10.1006/bbrc.1999.1934

subject

Has Abstract

pub_date

2000-02-05 00:00:00

pages

155-63

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(99)91934-4

journal_volume

268

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Structure of the gene encoding the human cyclin-dependent kinase inhibitor p18 and mutational analysis in breast cancer.

    abstract：:The cyclin-dependent kinase (CDK) inhibitor p18 blocks progression of the cell cycle by associating with the cyclin D-dependent kinases CDK6 and CDK4. To better understand the regulation of p18 gene expression, we isolated full-length cDNA clones from a human BT-20 breast cancer cell cDNA library. These clones were th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8497

    authors： Blais A,Labrie Y,Pouliot F,Lachance Y,Labrie C

    更新日期：1998-06-09 00:00:00

  • Estimation of the lipophilicity of anti-HIV nucleoside analogues by determination of the partition coefficient and retention time on a Lichrospher 60 RP-8 HPLC column.

    abstract：:There is a close linear correlation between the log partition coefficient (Pa) of a series of 2'-deoxyriboside (dR), 2',3'-didehydro-3'-dideoxyriboside (ddeR), 2',3'-dideoxyriboside (ddR), 3'-fluoro-2',3'-dideoxyriboside (FddR) and 3'-azido-2',3'-dideoxyriboside (AzddR) derivatives of uracil, cytosine, thymine, guanin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(89)80063-4

    authors： Balzarini J,Cools M,De Clercq E

    更新日期：1989-01-31 00:00:00

  • Isolation of two biologically active peptides, erythrotropin I and erythrotropin II from fetal calf intestine.

    abstract：:A bioassay based on the measurement of thymidine incorporation into trichloroacetic acid-insoluble materials in erythroid cell suspensions from fetal calf liver was used as the assay for purification of two small peptides (erythrotropins I and II) from fetal calf intestine. The peptides were purified using reversed-ph...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80169-7

    authors： Congote LF

    更新日期：1983-09-15 00:00:00

  • R-spondin 2 facilitates differentiation of proliferating chondrocytes into hypertrophic chondrocytes by enhancing Wnt/β-catenin signaling in endochondral ossification.

    abstract：:Endochondral ossification is a crucial process for longitudinal growth of bones. Differentiating chondrocytes in growth cartilage form four sequential zones of proliferation, alignment into column, hypertrophy, and substitution of chondrocytes with osteoblasts. Wnt/β-catenin signaling is essential for differentiation ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.03.089

    authors： Takegami Y,Ohkawara B,Ito M,Masuda A,Nakashima H,Ishiguro N,Ohno K

    更新日期：2016-04-22 00:00:00

  • Aggravation of brain infarction through an increase in acrolein production and a decrease in glutathione with aging.

    abstract：:We previously reported that tissue damage during brain infarction was mainly caused by inactivation of proteins by acrolein. This time, it was tested why brain infarction increases in parallel with aging. A mouse model of photochemically induced thrombosis (PIT) was studied using 2, 6, and 12 month-old female C57BL/6 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.03.137

    authors： Uemura T,Watanabe K,Ishibashi M,Saiki R,Kuni K,Nishimura K,Toida T,Kashiwagi K,Igarashi K

    更新日期：2016-04-29 00:00:00

  • HSP70 interacts with Rheb, inhibiting mTORC1 signaling.

    abstract：:The small GTPase Rheb binds and activates mTORC1, which plays a pivotal role in diverse cellular physiologies. To increase our understanding of how Rheb regulates mTORC1 signaling, we set out to identify Rheb binding proteins using shotgun proteomics approaches. In this study, we characterized HSP70, one of the identi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.07.053

    authors： Ryu HH,Ha SH

    更新日期：2020-12-17 00:00:00

  • Dissociation between the inhibitory and stimulatory effects of opioid peptides on cAMP formation in SK-N-SH neuroblastoma cells.

    abstract：:Opioid agonists either potentiate or suppress basal cAMP production in SK-N-SH cells. The inhibitory effect is mediated by PTX-sensitive GTP-binding proteins, while the stimulatory effect involves Ca++ entry and calmodulin activation. Both pathways can be activated simultaneously by opioid agonists. Low (nM) concentra...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8582

    authors： Sarne Y,Rubovitch V,Fields A,Gafni M

    更新日期：1998-05-08 00:00:00

  • Characterization of Erwinia carotovora subsp. carotovora LY34 endo-1,4-beta-glucanase genes and rapid identification of their gene products.

    abstract：:Genomic DNA of the phytopathogenic Erwinia carotovora subsp. carotovora LY34 was partially digested with Sau3AI, ligated into the BamHI site of pBlue-script II SK+, and introduced into E. coli. Two clones that were able to hydrolyse carboxymethylcellulose were selected. 1.5 kb and 1.2 kb fragments containing the celA ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7747

    authors： Park YW,Lim ST,Cho SJ,Yun HD

    更新日期：1997-12-29 00:00:00

  • MAP2 competes with MAP1 for binding to microtubules.

    abstract：:A question whether MAP1 and MAP2 (the major microtubule associated proteins from mammalian brain) bind to common or distinct sites on the microtubule surface was studied. Microtubules were assembled from tubulin and MAP1 and then centrifuged through a layer of MAP2 solution under conditions where no repolymerization o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91635-8

    authors： Kuznetsov SA,Rodionov VI,Gelfand VI,Rosenblat VA

    更新日期：1984-02-29 00:00:00

  • A regulatory role of Wnt signaling pathway in the hematopoietic differentiation of murine embryonic stem cells.

    abstract：:One of the most important issues in stem cell research is to understand the regulatory mechanisms responsible for their differentiation. An extensive understanding of mechanism underlying the process of differentiation is crucial in order to prompt stem cells to perform a particular function after differentiation. To ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.09.206

    authors： Feng Z,Srivastava AS,Mishra R,Carrier E

    更新日期：2004-11-26 00:00:00

  • Transforming growth factor-beta1 induces matrix metalloproteinase-9 expression in human meningeal cells via ERK and Smad pathways.

    abstract：:Transforming growth factor (TGF)-beta1, a cytokine released into the cerebrospinal fluid (CSF) after intraventricular hemorrhage (IVH), stimulates the expression of the components of the extracellular matrix (ECM), which causes progressive ventricular dilatation by impaired CSF absorption. Matrix metalloproteinase-9 (...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.04.038

    authors： Okamoto T,Takahashi S,Nakamura E,Nagaya K,Hayashi T,Fujieda K

    更新日期：2009-06-12 00:00:00

  • Oct4 plays a crucial role in the maintenance of gefitinib-resistant lung cancer stem cells.

    abstract：:Several recent studies have suggested that cancer stem cells (CSCs) are involved in resistance to gefitinib in non-small cell lung cancer (NSCLC). Oct4, a member of the POU-domain transcription factor family, has been shown to be involved in CSC properties of various cancers. We previously reported that Oct4 and the p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.03.064

    authors： Kobayashi I,Takahashi F,Nurwidya F,Nara T,Hashimoto M,Murakami A,Yagishita S,Tajima K,Hidayat M,Shimada N,Suina K,Yoshioka Y,Sasaki S,Moriyama M,Moriyama H,Takahashi K

    更新日期：2016-04-22 00:00:00

  • Regulation of lipogenesis via BHLHB2/DEC1 and ChREBP feedback looping.

    abstract：:BHLHB2/DEC1 is a transcription factor implicated in cell proliferation, apoptosis, and metabolism, and is also known to play an important role in the regulation of the mammalian circadian rhythm. However, its precise role in metabolism remains unclear. We investigated the link between BHLHB2 and ChREBP, a glucose-acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.06.101

    authors： Iizuka K,Horikawa Y

    更新日期：2008-09-12 00:00:00

  • Decline of p300 contributes to cell senescence and growth inhibition of hUC-MSCs through p53/p21 signaling pathway.

    abstract：:Human umbilical cord-derived mesenchymal stromal cells (hUC-MSCs) in vitro expansion for long term may undergo epigenetic and genetic alterations that subsequently induce cellular senescence and associated growth inhibition. Increasing evidence implicated that aberrant histone acetylation modulates gene expression res...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.05.061

    authors： Li Y,Zhong H,Wu M,Tan B,Zhao L,Yi Q,Xu X,Pan H,Bi Y,Yang K

    更新日期：2019-07-12 00:00:00

  • Internalization of MUC1 by anti-MUC1 antibody from cell membrane through the macropinocytotic pathway.

    abstract：:MUC1 is a type I transmembrane glycoprotein aberrantly overexpressed in various cancer cells. It is thought to serve as a physical barrier from the extracellular environment and also as a receptor for various extracellular molecules. However, little is known about the fate of MUC1 during and after the interaction with...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.08.059

    authors： Hisatsune A,Kawasaki M,Nakayama H,Mikami Y,Miyata T,Isohama Y,Katsuki H,Kim KC

    更新日期：2009-10-30 00:00:00

  • Detection of soluble P-glycoprotein in culture media and extracellular fluids.

    abstract：:Multidrug resistance (MDR) is a unique phenomenon in cancer patients and is commonly associated with an overexpression of the human MDR gene mdr1, which encodes an energy-dependent Mr 180 kDa membrane bound protein, known as P-glycoprotein. P-glycoprotein serves as a membrane efflux to pump the drugs out of the cancer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2211

    authors： Chu TM,Lin TH,Kawinski E

    更新日期：1994-08-30 00:00:00

  • Identification of an atypical calcium-dependent calmodulin binding site on the C-terminal domain of GluN2A.

    abstract：:N-methyl-D-aspartate (NMDA) receptors are calcium-permeable ion channels assembled from four subunits that each have a common membrane topology. The intracellular carboxyl terminal domain (CTD) of each subunit varies in length, is least conserved between subunits, and binds multiple intracellular proteins. We defined ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.111

    authors： Bajaj G,Hau AM,Hsu P,Gafken PR,Schimerlik MI,Ishmael JE

    更新日期：2014-02-21 00:00:00

  • A computergraphic determination of the chemotactic peptide preferred conformation.

    abstract：:Replacement of leucine in the chemotactic peptide For-Met-Leu-Phe by the sterically constrained amino acids alpha-aminoisobutyric acid and aminocyclohexanecarboxylic acid affords compounds of equal or greater activity than the parent. NMR studies indicate that the parent compound is present as a beta-sheet in solution...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80287-0

    authors： Semus SF,Becker EL,Toniolo C,Freer RJ

    更新日期：1988-12-15 00:00:00

  • Astroglial Ca2+ signaling is generated by the coordination of IP3R and store-operated Ca2+ channels.

    abstract：:Astrocytes play key roles in the central nervous system and regulate local blood flow and synaptic transmission via intracellular calcium (Ca2+) signaling. Astrocytic Ca2+ signals are generated by multiple pathways: Ca2+ release from the endoplasmic reticulum (ER) via the inositol 1, 4, 5-trisphosphate receptor (IP3R)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.096

    authors： Sakuragi S,Niwa F,Oda Y,Mikoshiba K,Bannai H

    更新日期：2017-05-13 00:00:00

  • Silencer-mediated repression and non-mediated activation of BDNF and c-fos gene promoters in primary glial or neuronal cells.

    abstract：:Although the neuron-restrictive silencer element (NRSE/Regard) has been shown to function as a negative-acting DNA regulatory element to prevent the expression of neuron-specific genes in non-neuronal cells, little is known about its silencing effect on transcription in primary glial cells nor its effect on transcript...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0974

    authors： Tabuchi A,Nakatani C,Nakaoka R,Naruse Y,Kojima T,Mori N,Tsuda M

    更新日期：1999-08-02 00:00:00

  • Active fragments and analogs of the insect neuropeptide leucopyrokinin: structure-function studies.

    abstract：:Evaluation of analogs of the blocked insect myotropic neuropeptide leucopyrokinin (LPK) has demonstrated its relative insensitivity to amino acid substitution in the N-terminal in contrast to the C-terminal region. Truncated analogs of LPK without the first, second, and third N-terminal amino acids retain a significan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90315-3

    authors： Nachman RJ,Holman GM,Cook BJ

    更新日期：1986-06-30 00:00:00

  • Two cis-acting regulatory sequences in the peroxisome proliferator-responsive enhancer region of rat acyl-CoA oxidase gene.

    abstract：:To clarify the mechanism of transcriptional induction of the rat liver acyl-CoA oxidase gene by hypolipidemic agents, we searched for cis-acting regulatory sequences in the 5' upstream region of the gene by transfection studies. We found that the sequence between -639 and -472 acts as a peroxisome proliferator-respons...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91645-s

    authors： Osumi T,Wen JK,Hashimoto T

    更新日期：1991-03-29 00:00:00

  • A natural component from Euphorbia humifusa Willd displays novel, broad-spectrum anti-influenza activity by blocking nuclear export of viral ribonucleoprotein.

    abstract：:The need to develop anti-influenza drugs with novel antiviral mechanisms is urgent because of the rapid rate of antigenic mutation and the emergence of drug-resistant viruses. We identified a novel anti-influenza molecule by screening 861 plant-derived natural components using a high-throughput image-based assay that ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.01.123

    authors： Chang SY,Park JH,Kim YH,Kang JS,Min JY

    更新日期：2016-03-04 00:00:00

  • Decreased cpg15 augments oxidative stress in sleep deprived mouse brain.

    abstract：:Sleep deprivation (SD) has detrimental effects on the physiological function of the brain. However, the underlying mechanism remains elusive. In the present study, we investigated the expression of candidate plasticity-related gene 15 (cpg15), a neurotrophic gene, and its potential role in SD using a REM-SD mouse mode...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.132

    authors： Li CJ,Li JJ,Jiang Y,Mu YW,Lu DX,Xiao ZY,Jiang HY,Zhao JJ,Chen XH

    更新日期：2020-02-12 00:00:00

  • Extracellular-regulated-kinase 5-mediated renal protection against ischemia-reperfusion injury.

    abstract：:ERK5, a member of the mitogen activated protein kinase, expressed in the kidneys was smaller (∼80kDa) in apparent molecular mass compared to other organs (∼120kDa). A blocking peptide experiment confirmed that the ∼80kDa detected on Western blots was a specific band detected by the anti-ERK5 antibody. Expression of th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.01.043

    authors： Kawakami T,Park SW,Kaku R,Yang J

    更新日期：2012-02-24 00:00:00

  • Rad51-related changes in global gene expression.

    abstract：:High expression of Rad51, the catalytic component in homologous recombination, has been reported to contribute to genomic instability. To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels. The results indic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.185

    authors： Orre LM,Fält S,Szeles A,Lewensohn R,Wennborg A,Flygare J

    更新日期：2006-03-10 00:00:00

  • Extraction of phosphorylated sperm specific histone H1 from sea urchin eggs: analysis of phosphopeptide maps.

    abstract：:Sea urchin spermatozoa contain a unique histone H1 (SpH1) with an unusual primary structure. Within 12 minutes postinsemination SpH1 is phosphorylated and lost from the chromatin. Both sperm and egg kinases phosphorylate SpH1 in vivo. Until now the analysis of the phosphorylation of SpH1 in eggs in vivo during the fir...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80493-5

    authors： Porter DC,Vacquier VD

    更新日期：1988-03-30 00:00:00

  • Differential effects of two-pore channel protein 1 and 2 silencing in MDA-MB-468 breast cancer cells.

    abstract：:Two-pore channel proteins, TPC1 and TPC2, are calcium permeable ion channels found localized to the membranes of endolysosomal calcium stores. There is increasing interest in the role of TPC-mediated intracellular signaling in various pathologies; however their role in breast cancer has not been extensively evaluated....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.06.127

    authors： Jahidin AH,Stewart TA,Thompson EW,Roberts-Thomson SJ,Monteith GR

    更新日期：2016-09-02 00:00:00

  • High level expression of human alpha-fetoprotein in transgenic mice.

    abstract：:Transgenic mice were produced by the microinjection of the cDNA of human alpha-fetoprotein (AFP) under control of the enhancer/promoter of the human beta-actin gene. Among the 4 mouse lines where the transgene were stable transmitted to the progeny, 3 produced human AFP. The expression was not developmental stage-spec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1276

    authors： Yamashita T,Kasai N,Miyoshi I,Sasaki N,Maki K,Sakai M,Nishi S,Namioka S

    更新日期：1993-03-15 00:00:00

  • FGF7 supports hematopoietic stem and progenitor cells and niche-dependent myeloblastoma cells via autocrine action on bone marrow stromal cells in vitro.

    abstract：:FGF1 and FGF2 support hematopoietic stem and progenitor cells (HSPCs) under stress conditions. In this study, we show that fibroblast growth factor (FGF7) may be a novel niche factor for HSPC support and leukemic growth. FGF7 expression was attenuated in mouse embryonic fibroblasts (MEFs) deficient for the MED1 subuni...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.09.044

    authors： Ishino R,Minami K,Tanaka S,Nagai M,Matsui K,Hasegawa N,Roeder RG,Asano S,Ito M

    更新日期：2013-10-11 00:00:00