Genome-wide screening by cDNA microarray of genes associated with matrix mineralization by human mesenchymal stem cells in vitro.

abstract：:Ultrasound (US) has emerged as a promising noninvasive modality for neuromodulation. Despite previous evidence that US may mediate cellular response by activating mechanosensitive ion channels embedded in the cell membrane, the underlying mechanism is not well understood. In this work, we developed a vertically deploy...

journal_title：Biochemical and biophysical research communications

authors： Liao D,Li F,Lu D,Zhong P

更新日期：2019-10-20 00:00:00

abstract：:Autophagy is the bulk degradation of cytosolic materials in lysosomes/vacuoles of eukaryotic cells. In the yeast Saccharomyces cerevisiae, 17 Atg proteins are known to be involved in autophagosome formation. Genome wide analyses have shown that Atg17 interacts with numerous proteins. Further studies on these interacti...

journal_title：Biochemical and biophysical research communications

authors： Kabeya Y,Kawamata T,Suzuki K,Ohsumi Y

更新日期：2007-05-04 00:00:00

abstract：:The rat adult liver and hepatocyte lines express an 1.7-kb variant alpha-fetoprotein (AFP) mRNA which differs from the 2.2-kb fetal AFP transcript in sequence in the 5' region. Here we report the characterization of a variant AFP cDNA, ARFP9, which is 1349 bp in length and encodes a 325 amino acid polypeptide. Nucleot...

journal_title：Biochemical and biophysical research communications

authors： Watanabe T,Jimenez-Molina JL,Chou JY

更新日期：1992-06-15 00:00:00

abstract：:Since estrogens strongly suppress the expression of alpha 2u-globulin genes in the rat liver, we studied the binding of estrogen-receptor complexes to fragments derived from alpha 2u-globulin gene RAO 01 using a DNA-cellulose competition assay. Rat uterus cytosol labelled with [3H]estradiol was used as a source of the...

journal_title：Biochemical and biophysical research communications

authors： Van Dijck P,Verhoeven G

更新日期：1992-01-15 00:00:00

abstract：:The Cyclin D3 protein is a member of the D-type cyclins. Besides serving as cell cycle regulators, D-type cyclins have been reported to be able to interact with several transcription factors and modulate their transcriptional activations. Here we report that human activating transcription factor 5 (hATF5) is a new int...

journal_title：Biochemical and biophysical research communications

authors： Liu W,Sun M,Jiang J,Shen X,Sun Q,Liu W,Shen H,Gu J

更新日期：2004-09-03 00:00:00

abstract：:Beta A3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the beta A3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that ...

journal_title：Biochemical and biophysical research communications

authors： McDermott JB,Cvekl A,Piatigorsky J

更新日期：1996-04-25 00:00:00

abstract：:Human skin fibroblasts incubated in lipoprotein-deficient medium in the presence of 50-100 microM of the calcium channel blockers verapamil or diltiazem incorporated up to 2.5 times more [35S]methionine into immunoprecipitable LDL receptor protein than did control cells. Verapamil was found to be more potent in this r...

journal_title：Biochemical and biophysical research communications

authors： Filipovic I,Buddecke E

更新日期：1986-05-14 00:00:00

abstract：:RT-PCR, a rapidly emerging technique for the detection of RNA, is being used by many investigators to quantify small amounts of RNA. Accurate quantification of RNA content has been facilitated by the use of competitive amplicons as internal controls. We demonstrate that losses in sensitivity and accuracy are associate...

journal_title：Biochemical and biophysical research communications

authors： Henley WN,Schuebel KE,Nielsen DA

更新日期：1996-09-04 00:00:00

abstract：:Bromodomains are epigenetic readers that specifically bind to the acetyl lysine residues of histones and transcription factors. Small molecule BET bromodomain inhibitors can disrupt this interaction which leads to potential modulation of several disease states. Here we describe the binding properties of a novel BET in...

journal_title：Biochemical and biophysical research communications

authors： Kharenko OA,Gesner EM,Patel RG,Norek K,White A,Fontano E,Suto RK,Young PR,McLure KG,Hansen HC

更新日期：2016-08-12 00:00:00

abstract：:UCP-2 is a member of the emerging family of UCP homologues. Upon high-fat feeding, UCP-2 mRNA levels are increased in epididymal fat pads of A/J mice, suggesting that the flux of fatty acids entering adipose tissue may regulate UCP-2 gene expression. Since fatty acids act as positive transcriptional regulators of lipi...

journal_title：Biochemical and biophysical research communications

authors： Aubert J,Champigny O,Saint-Marc P,Negrel R,Collins S,Ricquier D,Ailhaud G

更新日期：1997-09-18 00:00:00

abstract：:In lens, αβγ-crystallins accounting for ∼90% of ocular proteins with concentrations >400 mg/ml need to remain soluble for the whole life-span and their aggregation can lead to cataract. Mysteriously, despite being a metabolically-quiescent organ, lens maintains ATP concentrations of 3-7 mM. Very recently, ATP was prop...

journal_title：Biochemical and biophysical research communications

authors： He Y,Kang J,Song J

更新日期：2020-06-11 00:00:00

abstract：:The proteins belonging to SWI2/SNF2 family of DNA dependent ATPases are important members of the chromatin remodeling complexes that are implicated in epigenetic control of gene expression. We have identified a human gene with a putative DNA binding domain, which belongs to the INO80 subfamily of SWI2/SNF2 proteins. H...

journal_title：Biochemical and biophysical research communications

authors： Bakshi R,Mehta AK,Sharma R,Maiti S,Pasha S,Brahmachari V

更新日期：2006-01-06 00:00:00

abstract：:The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultr...

journal_title：Biochemical and biophysical research communications

authors： Dossymbekova R,Bgatova N,Tungushbayeva Z,Sharipov K,Taneyeva G,Kydyrbaeva A,Solovieva A

更新日期：2020-07-23 00:00:00

abstract：:In the present study, we demonstrated that Ang II provokes a transitory enhancement of focal adhesion kinase (FAK) and paxillin phosphorylation in human umbilical endothelial cells (HUVEC). Moreover, Ang II induces a time- and dose-dependent augmentation in cell migration, but does not affect HUVEC proliferation. The ...

journal_title：Biochemical and biophysical research communications

authors： Montiel M,de la Blanca EP,Jiménez E

更新日期：2005-02-25 00:00:00

abstract：:Nitrosporeusine A (NA) has been recently reported to exert anti-inflammatory and renal protective effects, but whether NA can attenuate sepsis-associated acute kidney injury (AKI) has not yet been reported. In this study, our results found that cecal ligation and puncture (CLP) reduced renal PGC-1α expression and indu...

journal_title：Biochemical and biophysical research communications

authors： Zhao Y,Nie M,Xu P,Li H,Xu L,Cheng XQ,Lei YQ

更新日期：2019-07-30 00:00:00

abstract：:Vasoactive intestinal contractor (VIC) is a member of the endothelin (ET) family. We have investigated the regional distribution of VIC/ET-2 and of ET-1 gene expression in the adult murine brain and pituitary gland. We used real-time quantitative reverse transcription-linked polymerase chain reaction. VIC/ET-2 gene ex...

journal_title：Biochemical and biophysical research communications

authors： Masuo Y,Ishikawa Y,Kozakai T,Uchide T,Komatsu Y,Saida K

更新日期：2003-01-17 00:00:00

abstract：:In the presence of UDPglucose, rabbit muscle phosphofructokinase appeared to use PPi as a phosphoryl donor, as reported previously (Biochem. Biophys. Res. Commun. 121, 842-847). This apparent activity was due to conversion of UDPglucose and PPi to glucose 1-phosphate and UTP, the latter being metabolized by phosphofru...

journal_title：Biochemical and biophysical research communications

authors： Kruger NJ,Dennis DT

更新日期：1985-01-16 00:00:00

abstract：:The role of small ras homologous GTP-binding proteins in the regulation of smooth muscle contractility has become increasingly apparent but there is still little information about the presence of these proteins in human uterine smooth muscle. Messenger RNAs for p21-activated protein kinase isoforms (PAK1, PAK2, and PA...

journal_title：Biochemical and biophysical research communications

authors： Moore F,Da Silva C,Wilde JI,Smarason A,Watson SP,López Bernal A

更新日期：2000-03-16 00:00:00

abstract：:Heparin-binding EGF-like growth factor (HB-EGF) is a polypeptide with an apparent molecular weight of 22 kilodalton that is related to epidermal growth factor (EGF) and that binds and activates the EGF receptor. We examined HB-EGF biological action and expression in human pancreatic cancer cell lines, and compared HB-...

journal_title：Biochemical and biophysical research communications

authors： Kobrin MS,Funatomi H,Friess H,Buchler MW,Stathis P,Korc M

更新日期：1994-08-15 00:00:00

abstract：:Polarization of epithelial cells requires vectorial sorting and transport of polarity proteins to apical or basolateral domains. Kif5b is the mouse homologue of the human ubiquitous Kinesin Heavy Chain (uKHC). To investigate the function of Kif5b in epithelial cells, we examined the phenotypes of Kif5b-deficient MDCK ...

journal_title：Biochemical and biophysical research communications

authors： Cui J,Jin G,Yu B,Wang Z,Lin R,Huang JD

更新日期：2015-07-17 00:00:00

abstract：:The nonamer 5'd(CTCAGCCTC) 3' 1 has been reacted with cis-diamminediaquaplatinum(II) in water at pH 4.2. The major reaction product was shown by enzymatic digestion and 1H NMR to be the d(ApG)cis-Pt(NH3)2 chelate [cis-Pt(NH3)2[d(CTCAGCCTC)-N7(4),N7(5)]] 1-Pt. When mixed with its complementary strand 2, 1-Pt forms a B ...

journal_title：Biochemical and biophysical research communications

authors： Fouchet MH,Gauthier C,Guittet E,Girault JP,Igolen J,Chottard JC

更新日期：1992-01-31 00:00:00

abstract：:Wet elastoidin spicules (fish fin rays) yield low-angle meridional X-ray diffraction patterns which resemble those from tendons. However, when the spicule dries the meridian splits into the arms of a diagonal cross (sometimes only one arm appears). Of the possible explanations we reject shearing of the axial arrangeme...

journal_title：Biochemical and biophysical research communications

authors： Hukins DW,Woodhead-Galloway J,Knight DP

更新日期：1976-12-20 00:00:00

abstract：:Previously, we have reported that two clustered cat genes from Acenitobacter lwoffi K24 had different arrangements, catB1C1A1 and catB2A2C2 (Kim, S.I., S.-H. Leem, J.-S. Choi, Y.H. Chung, S. Kim, Y.-M. Park, Y.K. Park, Y.N. Lee, and K.-S. Ha. 1997, J. Bacteriol. 179, 5226-5231). By further analysis of the organization...

journal_title：Biochemical and biophysical research communications

authors： Kim SI,Leem SH,Choi JS,Ha KS

更新日期：1998-02-04 00:00:00

abstract：OBJECTIVE:This study aimed to evaluate functions of APOBEC3F gene in biological process of hepatocellular carcinoma (HCC) and anti-tumor mechanisms of bufalin. METHODS:Effect of APOBEC3F and bufalin on cell proliferation and migration abilities were evaluated by CCK-8, wounding healing tests and transwell assays in SK...

journal_title：Biochemical and biophysical research communications

authors： Yang Z,Tao Y,Xu X,Cai F,Yu Y,Ma L

更新日期：2018-09-10 00:00:00

abstract：:We constructed a system for expressing the Fab of the therapeutic human monoclonal antibody adalimumab at a yield of 20 mg/L in the methylotrophic yeast Pichia pastoris. To examine the contribution of interchain disulfide bonds to conformational stability, we prepared adalimumab Fab from which the interchain disulfide...

journal_title：Biochemical and biophysical research communications

authors： Nakamura H,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2018-01-01 00:00:00

abstract：:One of the most important issues in stem cell research is to understand the regulatory mechanisms responsible for their differentiation. An extensive understanding of mechanism underlying the process of differentiation is crucial in order to prompt stem cells to perform a particular function after differentiation. To ...

journal_title：Biochemical and biophysical research communications

authors： Feng Z,Srivastava AS,Mishra R,Carrier E

更新日期：2004-11-26 00:00:00

abstract：:Our goal was to characterize the glucose transporter in synaptosomes and to compare it to the different forms of transporter already identified. Cross-reactivity with antibodies to the human erythrocyte transporter, Km of glucose uptake, reversibility of NEM inhibition of transport, and insulin sensitivity were all ex...

journal_title：Biochemical and biophysical research communications

authors： Bhattacharyya MV,Brodsky JL

更新日期：1988-09-15 00:00:00

abstract：:Recently, circular RNAs (circRNAs) are identified as a novel class of noncoding RNAs playing important roles in human malignant tumors. However, the regulatory function of circRNA in lung adenocarcinoma (LUAD) is still largely unknown. Present study aimed to explore the role of circ_0006427 in LUAD progression. Firstl...

journal_title：Biochemical and biophysical research communications

authors： Yao Y,Hua Q,Zhou Y

更新日期：2019-01-01 00:00:00

abstract：:In this study we investigated the role of active site residues in the peroxidase activity of Orp1 (GPx3) using three different peroxide substrates. Using a structural homology model of the reduced form of Orp1, we identified Asn126 and Phe127 as evolutionarily conserved residues that line the back of the Orp1 active s...

journal_title：Biochemical and biophysical research communications

authors： Takanishi CL,Ma LH,Wood MJ

更新日期：2010-12-03 00:00:00

abstract：:The crystal structure of the mu and kappa opioid receptors has revealed dimeric structural arrangements. Mu-delta receptors heteromers also exist and we have identified discrete cytoplasmic regions in each receptor required for oligomer formation. In the carboxyl tail of the delta receptor we identified three glycine ...

journal_title：Biochemical and biophysical research communications

authors： O'Dowd BF,Ji X,O'Dowd PB,Nguyen T,George SR

更新日期：2012-06-15 00:00:00