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Effect of lithium carbonate on autophagy and proliferative activity of isolated hepatocytes.

Abstract：

:The hepatocytes were cultivated in the presence of lithium carbonate (LC) for drugs testing or possible source for transplantation in the treatment of hereditary or terminal liver diseases. The LC, as an inducer of autophagy, is a promising drug for maintaining cell homeostasis and has a significant effect on the ultrastructural organization of hepatocyte cells. Within current investigation, new mechanisms of the biological effects of lithium and the ultrastructural analysis of the primary culture of hepatocytes were studied via flow cytofluorometry, light, and electron microscopy methods. Obtained results demonstrate the absence of the toxic effect of 5 mM of LC on the primary hepatocyte culture. In addition, LC does not block the cell cycle at the G0/G1 stage after 24 h of hepatocyte cultivation and promotes the preservation of their viability by 48 h of the experiment. Moreover, LC does not stimulate hepatocyte apoptosis, induces autophagy and the preserves the proliferative activity of hepatocytes.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Dossymbekova R,Bgatova N,Tungushbayeva Z,Sharipov K,Taneyeva G,Kydyrbaeva A,Solovieva A

doi

10.1016/j.bbrc.2020.03.057

subject

Has Abstract

pub_date

2020-07-23 00:00:00

pages

343-346

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(20)30524-6

journal_volume

528

pub_type

杂志文章

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