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Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells.

Abstract：

:Human embryonic stem cells (hESCs) are thought to be a promising cell source for cell transplantation therapy. For such a clinical application, the hESCs should be manipulated using appropriate and qualified materials. In this study, we examined the efficacy of recombinant human laminin (rhLM) isoforms on the undifferentiated growth of hESCs. We first determined the major integrins expressed on the hESCs to reveal the preference of the hESCs for rhLMs, and found that the hESCs mainly expressed integrin alpha6beta1, which binds predominantly to laminin-111, -332 and -511/-521. When the hESCs were seeded onto rhLMs, the cells indeed adhered markedly to rhLM-332, and to rhLM-511 and rhLM-111 to a lesser extent. The hESCs proliferated on these three rhLMs for several passages while preserving their pluripotency. These results show that rhLM-111, -332, and -511 are good substrates to expand undifferentiated hESCs due to their high affinity to integrin alpha6beta1 expressed on hESCs.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Miyazaki T,Futaki S,Hasegawa K,Kawasaki M,Sanzen N,Hayashi M,Kawase E,Sekiguchi K,Nakatsuji N,Suemori H

doi

10.1016/j.bbrc.2008.07.111

subject

Has Abstract

pub_date

2008-10-10 00:00:00

pages

27-32

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(08)01431-9

journal_volume

375

pub_type

杂志文章

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