当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts.

Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts.

Abstract：

:A monoclonal antibody, highly selective for the 5'-phosphopyridoxyl group, can be used to detect cytosolic pyridoxal-5'-phosphate binding proteins by an immunoblot procedure. This technique, when applied to sodium borohydride-treated cytosolic extracts obtained from normal rat liver at various stages of development as well as several liver-derived Morris hepatomas, reveals patterns of pyridoxal-5'-phosphate binding proteins that are characteristic of the various sources of cytosol. These findings suggest that there are developmental and tumor-specific requirements for pyridoxal-5'-phosphate, the coenzymatically active form of vitamin B-6.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kittler JM,Viceps-Madore D,Cidlowski JA,Thanassi JW

doi

10.1016/0006-291x(83)91797-7

subject

Has Abstract

pub_date

1983-04-15 00:00:00

pages

61-5

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(83)91797-7

journal_volume

112

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Sulfiredoxin-1 alleviates high glucose-induced podocyte injury though promoting Nrf2/ARE signaling via inactivation of GSK-3β.

    abstract：:Hyperglycemia-induced podocyte injury plays a vital role in the development of diabetic nephropathy. Sulfiredoxin-1 (Srxn1) is emerging as a cytoprotective protein that protects from various insults in a wide range of cell types. However, whether Srxn1 is involved in regulating hyperglycemia-induced podocyte injury an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.06.157

    authors： Shen Y,Chen S,Zhao Y

    更新日期：2019-09-03 00:00:00

  • Reversion of 7-methylguanosine 5'-phosphate inhibition of mRNA translation by polysomal and soluble factors isolated from Saccharomyces cerevisiae.

    abstract：:Protein fractions that overcome m7GMP inhibition of mRNA translation have been purified from the yeast S. cerevisiae. An active fraction isolated from polysomes contains two polypeptides of 220- and 190-kDa. The active fraction isolated from postribosomal supernatant contains a major polypeptide of 28-kDa and other sp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90760-1

    authors： Parets Suler A,Zueco J,Gozalbo D,Sentandreu R

    更新日期：1987-08-14 00:00:00

  • Phosphorylation of voltage-dependent anion channel by c-Jun N-terminal Kinase-3 leads to closure of the channel.

    abstract：:Stress activated c-Jun N-terminal Kinase-3 (JNK3) has been reported to act on mitochondrion to promote neuronal cell death. Phosphorylation of mitochondrial Voltage-Dependent Anion Channel (VDAC) plays an important role in mitochondria-mediated cell death. Keeping these in view phosphorylation of rat brain VDAC by JNK...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.02.077

    authors： Gupta R,Ghosh S

    更新日期：2015-03-27 00:00:00

  • Cysteine-rich secretory protein 3 inhibits hepatitis C virus at the initial phase of infection.

    abstract：:Hepatitis C virus (HCV) affects 2-3% of the global population. Approximately one-quarter of acute infections cause chronic hepatitis that leads to liver cirrhosis or hepatocellular carcinoma. The major obstacle of current research is the extremely narrow host tropism of HCV. A single HCV strain can replicate in the Hu...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.06.106

    authors： Lee U,Nam YR,Ye JS,Lee KJ,Kim N,Joo CH

    更新日期：2014-07-25 00:00:00

  • Elafin is a potent inhibitor of proteinase 3.

    abstract：:Elafin, a human skin derived inhibitor of human leukocyte elastase, was tested for inhibitory activity against proteinase 3, an elastin degrading proteinase of neutrophils. The inhibitory activity of elafin was compared with antileukoprotease and eglin C. Elafin proved to be a potent inhibitor of elastin-FITC degradat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90476-n

    authors： Wiedow O,Lüademann J,Utecht B

    更新日期：1991-01-15 00:00:00

  • A valine-to-lysine substitution at position 210 induces structural conversion of prion protein into a β-sheet rich oligomer.

    abstract：:Prion diseases are fatal neurodegenerative diseases associated with structural conversion of α-helical prion protein (PrP) into its β-sheet rich isoform (PrPSc). Previous genetic analyses have indicated that several amino acid residues involved in the hydrophobic core of PrP (such as V180, F198, and V210) play a criti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.10.075

    authors： Kakuda K,Yamaguchi KI,Kuwata K,Honda R

    更新日期：2018-11-17 00:00:00

  • Structural differences in the ability of lysophospholipids to inhibit endothelium-dependent hyperpolarization by acetylcholine in rat mesenteric arteries.

    abstract：:The effects of different lysophospholipids on endothelium-dependent hyperpolarization by acetylcholine were examined in rat mesenteric arteries. Lysophosphatidylcholine with 14 or longer carbon acyl chain significantly inhibited the hyperpolarization, while that with 12 or lesser carbon acyl chain was without effect. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1532

    authors： Fukao M,Hattori Y,Kanno M,Sakuma I,Kitabatake A

    更新日期：1996-10-14 00:00:00

  • Carbohydrate composition of serum transferrin isoforms from patients with high alcohol consumption.

    abstract：:Normal human serum transferrin is present in several isoforms, due to differences in glycosylation. Transferrin has two potential glycosylation sites, both normally occupied by oligosaccharide chains. Two of the transferrin isoforms, called carbohydrate deficient transferrin, are specifically increased in patients wit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1656

    authors： Landberg E,Påhlsson P,Lundblad A,Arnetorp A,Jeppsson JO

    更新日期：1995-05-16 00:00:00

  • Biological activities of rabbit antibodies against synthetic human thyrotropin receptor peptides representing thyrotropin binding regions.

    abstract：:Recently, we have shown that the thyrotropin (TSH) binding regions of human thyrotropin receptor (TSHR) reside in two areas within residues 12-44 and 308-344. Serial antisera were raised against four overlapping synthetic peptides representing these two regions of TSHR (peptides 12-30, 24-44, 308-328, and 324-344) and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91884-s

    authors： Sakata S,Ogawa T,Matsui I,Manshouri T,Atassi MZ

    更新日期：1992-02-14 00:00:00

  • Histone chaperones cooperate to mediate Mef2-targeted transcriptional regulation during skeletal myogenesis.

    abstract：:Histone chaperones function in histone transfer and regulate the nucleosome occupancy and the activity of genes. HIRA is a replication-independent (RI) histone chaperone that is linked to transcription and various developmental processes. Here, we show that HIRA interacts with Mef2 and contributes to the activation of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.03.055

    authors： Yang JH,Choi JH,Jang H,Park JY,Han JW,Youn HD,Cho EJ

    更新日期：2011-04-15 00:00:00

  • Physiological validation of photochemical reflectance index (PRI) as a photosynthetic parameter using Arabidopsis thaliana mutants.

    abstract：:Non-photochemical quenching (NPQ) is the most important photoprotective system in higher plants. NPQ can be divided into several steps according to the timescale of relaxation of chlorophyll fluorescence after reaching a steady state (i.e., the fast phase, qE; middle phase, qZ or qT; and slow phase, qI). The dissipati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.02.192

    authors： Kohzuma K,Hikosaka K

    更新日期：2018-03-25 00:00:00

  • Kinesin I and cytoplasmic dynein orchestrate glucose-stimulated insulin-containing vesicle movements in clonal MIN6 beta-cells.

    abstract：:Glucose-stimulated mobilization of large dense-core vesicles (LDCVs) to the plasma membrane is essential for sustained insulin secretion. At present, the cytoskeletal structures and molecular motors involved in vesicle trafficking in beta-cells are poorly defined. Here, we describe simultaneous imaging of enhanced gre...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.208

    authors： Varadi A,Tsuboi T,Johnson-Cadwell LI,Allan VJ,Rutter GA

    更新日期：2003-11-14 00:00:00

  • Serine/threonine/tyrosine-interacting-like protein 1 (STYXL1), a pseudo phosphatase, promotes oncogenesis in glioma.

    abstract：:Phosphatases play an important role in cellular signaling and are often found dysregulated in cancers including glioblastoma (GBM). A comprehensive bioinformatics analysis of phosphatases (n = 403) in multiple datasets revealed their deregulation in GBM. Among the differentially regulated phosphatases (n = 186; 46.1%)...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2019.05.093

    authors： Tomar VS,Baral TK,Nagavelu K,Somasundaram K

    更新日期：2019-07-12 00:00:00

  • Characterization of a competitive and reversible ligand to E2 conformation of Na, K ATPase molecule.

    abstract：:The binding of cardiac glycosides to the E2 conformation of Na, K ATPase suggests that this conformation may represent a potential regulatory site for an endogenous ligand. To examine this hypothesis, delipidated and desalted, ethanolic extracts were prepared from plasma. Plasma extracts produced displacement of 3H-ou...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90441-x

    authors： Rauch AL,Buckalew VM Jr

    更新日期：1988-01-29 00:00:00

  • Promoter structure of the mouse TATA-binding protein (TBP) gene.

    abstract：:5'-RACE and genomic cloning were used to determine that the mouse TBP (mTBP) gene consists of one 5'-terminal non-coding exon followed by seven protein-coding exons. The region upstream of the first exon lacks a TATA-box. Hence, as with the case of other genes carrying TATA-less promoter, transcription starts from a c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1166

    authors： Ohbayashi T,Schmidt EE,Makino Y,Kishimoto T,Nabeshima Y,Muramatsu M,Tamura T

    更新日期：1996-08-05 00:00:00

  • The alpha2-adrenoreceptor agonist dexmedetomidine protects against lipopolysaccharide-induced apoptosis via inhibition of gap junctions in lung fibroblasts.

    abstract：:The α2-adrenoceptor inducer dexmedetomidine protects against acute lung injury (ALI), but the mechanism of this effect is largely unknown. The present study investigated the effect of dexmedetomidine on apoptosis induced by lipopolysaccharide (LPS) and the relationship between this effect and gap junction intercellula...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.10.162

    authors： Zhang Y,Tan X,Xue L

    更新日期：2018-01-01 00:00:00

  • Quinone reduction by Rhodothermus marinus succinate:menaquinone oxidoreductase is not stimulated by the membrane potential.

    abstract：:Succinate:quinone oxidoreductase (SQR), a di-haem enzyme purified from Rhodothermus marinus, reveals an HQNO-sensitive succinate:quinone oxidoreductase activity with several menaquinone analogues as electron acceptors that decreases with lowering the redox midpoint potential of the quinones. A turnover with the low-po...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.03.015

    authors： Fernandes AS,Konstantinov AA,Teixeira M,Pereira MM

    更新日期：2005-05-06 00:00:00

  • Cloning and characterization of a novel RNA polymerase II C-terminal domain phosphatase.

    abstract：:Reversible phosphorylation of RNA polymerase (RNAP) II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism. The CTD phosphatase, FCP1, catalyzes the dephosphorylation of RNAP II and is thought to play a major role in polymerase recycling. In this study, we isolated a novel phosphatase gene ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.04.065

    authors： Zheng H,Ji C,Gu S,Shi B,Wang J,Xie Y,Mao Y

    更新日期：2005-06-17 00:00:00

  • The small-molecule IAP antagonist AT406 inhibits pancreatic cancer cells in vitro and in vivo.

    abstract：:In the present study, we tested the anti-pancreatic cancer activity by AT406, a small-molecule antagonist of IAP (inhibitor of apoptosis proteins). In established (Panc-1 and Mia-PaCa-2 lines) and primary human pancreatic cancer cells, treatment of AT406 significantly inhibited cell survival and proliferation. Yet, sa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.07.011

    authors： Jiang Y,Meng Q,Chen B,Shen H,Yan B,Sun B

    更新日期：2016-09-09 00:00:00

  • CYLD, a deubiquitinase specific for lysine63-linked polyubiquitins, accumulates at the postsynaptic density in an activity-dependent manner.

    abstract：:Polyubiquitin chains on proteins flag them for distinct fates depending on the type of polyubiquitin linkage. While lysine48-linked polyubiquitination directs proteins to proteasomal degradation, lysine63-linked polyubiquitination promotes different protein trafficking and is involved in autophagy. Here we show that p...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.10.131

    authors： Dosemeci A,Thein S,Yang Y,Reese TS,Tao-Cheng JH

    更新日期：2013-01-04 00:00:00

  • A new method for rapid assignment of S-S bridges in proteins.

    abstract：:A new method for complementing existing protein chemical techniques for the assignment of S-S bridge positions in amino-acid sequences is described. The principle of the method is the direct examination of the masses of protein fragments, obtained by chemical or enzymatic degradation. Proteins are digested under condi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90302-x

    authors： Morris HR,Pucci P

    更新日期：1985-02-15 00:00:00

  • The Tax oncogene enhances ELL incorporation into p300 and P-TEFb containing protein complexes to activate transcription.

    abstract：:The eleven-nineteen lysine-rich leukemia protein (ELL) is a key regulator of RNA polymerase II mediated transcription. ELL facilitates RNA polymerase II transcription pause site entry and release by dynamically interacting with p300 and the positive transcription elongation factor b (P-TEFb). In this study, we investi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.072

    authors： Fufa TD,Byun JS,Wakano C,Fernandez AG,Pise-Masison CA,Gardner K

    更新日期：2015-09-11 00:00:00

  • Adipocyte-derived microvesicles contain RNA that is transported into macrophages and might be secreted into blood circulation.

    abstract：:We have recently found that 3T3-L1 adipocytes secrete microvesicles, known as adipocyte-derived microvesicles (ADMs), with angiogenic activity. In this study, we found that ADMs contain RNA without typical 28S and 18S ribosomal RNA inside the vesicles. Microarray analysis revealed that ADMs contain approximately 7000 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.07.008

    authors： Ogawa R,Tanaka C,Sato M,Nagasaki H,Sugimura K,Okumura K,Nakagawa Y,Aoki N

    更新日期：2010-08-06 00:00:00

  • Enzymatic properties of the phosphorylated urokinase-type plasminogen activator isolated from a human carcinomatous cell line.

    abstract：:Enzymatic properties of phosphorylated urokinase plasminogen activator (P-uPA) (1) extracted from human carcinomatous cell line Detroit 562 cells were compared with those of non-phosphorylated uPA of urinary origin (nP-uPA). Using plasminogen as a substrate, the Km and Kcat of P-uPA were higher than that of nP-uPA whi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91900-b

    authors： Takahashi K,Kwaan HC,Koh E,Tanabe M

    更新日期：1992-02-14 00:00:00

  • Does intracellular histamine mediate mast cell histamine release?

    abstract：:Previously, we demonstrated that through binding a novel intracellular receptor of microM affinity (HIC), histamine mediates, and the HIC antagonist N,N-diethyl-2-[4-(phenylmethyl)phenoxy]ethanamine. HCl (DPPE) inhibits, platelet aggregation and serotonin granule secretion; the latter response is dependent upon the sa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)92077-d

    authors： Brandes LJ,Sukhu B,Bogdanovic RP

    更新日期：1990-03-16 00:00:00

  • Engineering of heterologous cytochrome P450 in Acinetobacter sp.: application for pollutant degradation.

    abstract：:Many organisms do not contain the necessary biochemical armoury to carry out the initial oxidative attack of many pollutant chemicals. In the present study, Acinetobacter sp. strain BD413 has been genetically engineered to express the cytochrome P450 xenobiotic-metabolising enzyme CYP105D1 from Streptomyces griseus th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3541

    authors： Lamb DC,Kelly DE,Masaphy S,Jones GL,Kelly SL

    更新日期：2000-09-24 00:00:00

  • Precocious induction of malic enzyme by nutritional and hormonal factors in rat foetal hepatocyte primary cultures.

    abstract：:Rat foetal hepatocytes in primary cultures were used as a model for the study of malic enzyme gene expression. Carbohydrates and glycolytic metabolites produced the precocious induction of the malic enzyme in foetal hepatocytes cultured in the absence of serum and hormones. Palmitate prevented this induction. Insulin ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91346-6

    authors： Fabregat I,Lorenzo M,Benito M

    更新日期：1989-06-30 00:00:00

  • Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism.

    abstract：:Very-long-chain acyl-CoA synthetases (VLCS) activate very-long-chain fatty acids (VLCFA) containing 22 or more carbons to their CoA derivatives. We cloned the human ortholog (hVLCS) of the gene encoding the rat liver enzyme (rVLCS). Both hVLCS and rVLCS contain 620 amino acids, are expressed primarily in liver and kid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.0510

    authors： Steinberg SJ,Wang SJ,Kim DG,Mihalik SJ,Watkins PA

    更新日期：1999-04-13 00:00:00

  • Interaction of the PDZ domain of human PICK1 with class I ADP-ribosylation factors.

    abstract：:We have cloned the cDNA encoding human PICK1 (protein interacting with C kinase 1), a PDZ domain-containing protein of 415 amino acids, and also identified the Drosophila homologue by search of the databank. Northern blot analysis shows a single mRNA of about 2.0 kb ubiquitously expressed in human tissues. Although PI...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1932

    authors： Takeya R,Takeshige K,Sumimoto H

    更新日期：2000-01-07 00:00:00

  • Neuronal nitric oxide synthase specific autophosphorylation in baculovirus/Sf9 insect cell system.

    abstract：:Neuronal nitric oxide synthase (nNOS) is a calmodulin (CaM)-dependent enzyme, which generates the ubiquitous biological mediator nitric oxide. In this study, wild-type or mutant form of rat nNOS was overexpressed in a baculovirus/Sf9 insect cell system and examined for autophosphorylation. Incubation of purified wild-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0286

    authors： Watanabe Y,Terashima K,Hidaka H

    更新日期：1996-02-15 00:00:00