Characterization of the glucose transporter from rat brain synaptosomes.

Abstract:

:Our goal was to characterize the glucose transporter in synaptosomes and to compare it to the different forms of transporter already identified. Cross-reactivity with antibodies to the human erythrocyte transporter, Km of glucose uptake, reversibility of NEM inhibition of transport, and insulin sensitivity were all examined. Immunoblotting showed a band at Mr 40,000, and the Km of glucose uptake was determined to be about 4 mM. Treatment with NEM caused irreversible inhibition of glucose uptake, while incubation with insulin failed to stimulate uptake. The results suggest that the transporter in synaptosomes resembles the human erythrocyte transporter.

authors

Bhattacharyya MV,Brodsky JL

doi

10.1016/s0006-291x(88)80549-7

subject

Has Abstract

pub_date

1988-09-15 00:00:00

pages

685-91

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(88)80549-7

journal_volume

155

pub_type

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