当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Isoprenylation of the low molecular mass GTP-binding proteins rac 1 and rac 2: possible role in membrane localization.

Isoprenylation of the low molecular mass GTP-binding proteins rac 1 and rac 2: possible role in membrane localization.

Abstract：

:Ras proteins can be modified at their COOH-terminal cysteine in the motif Cys-Ali-Ali-Xaa by a farnesyl isoprenoid. This modification is essential for membrane association and biological activity of ras proteins. A similar COOH-terminal amino acid sequence, Cys-Xaa-Ali-Xaa, exists in the ras-related GTP-binding proteins rac 1 and rac 2. To determine whether these proteins were similarly modified, COS cells were transfected with rac 1 and rac 2 cDNA and expressed proteins were labeled with [3H]mevalonic acid. We report here that both rac 1 and rac 2 are post-translationally modified by addition of an isoprenoid group, the likely site of which is the COOH-terminal cysteine. Isoprenylation was found only in racs associated with particulate cell fractions, suggesting that this modification may be associated with membrane localization of the proteins. These data specifically identify mammalian low molecular mass GTP-binding proteins other than ras that undergo post-translational modification and further define the COOH-terminal consensus sequence, Cys-Ali-Ali-Xaa, as an isoprenylation signal. This sequence may identify a larger family of low molecular mass GTP-binding proteins which are isoprenylated.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Didsbury JR,Uhing RJ,Snyderman R

doi

10.1016/0006-291x(90)91217-g

subject

Has Abstract

pub_date

1990-09-14 00:00:00

pages

804-12

issue

2

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)91217-G

journal_volume

171

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Electrospun protein-CNT composite fibers and the application in fibroblast stimulation.

    abstract：:Functional biopolymer scaffolds are in high demand for tissue regeneration. In this study, we incorporated functionalized CNT in collagen or silk protein solution to generate biocomposite fibers by electrospinning. The addition of CNT reinforced the strength of the scaffolds and rendered the fibers electrical conducti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.08.157

    authors： Chi N,Wang R

    更新日期：2018-09-26 00:00:00

  • ROR gamma: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle.

    abstract：:In this study, we describe the identification and cloning of a novel member of the nuclear receptor superfamily. This orphan receptor, referred to as ROR gamma, belongs to the ROR/RZR subfamily. The open reading frame of ROR gamma encodes a protein of 560 amino acid residues with a predicted molecular mass of 63 kD. T...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2902

    authors： Hirose T,Smith RJ,Jetten AM

    更新日期：1994-12-30 00:00:00

  • PGC-1 and PERC, coactivators of the estrogen receptor-related receptor gamma.

    abstract：:The mouse nuclear receptor ERRgamma (estrogen receptor-related receptor gamma) is highly expressed in heart, skeletal muscle, kidney, and brain, as well as in the developing nervous system. We found that the expression of the coactivators PGC-1 (PGC-1alpha) and PERC (PGC-1beta) in mammalian cells augmented potently th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02753-5

    authors： Hentschke M,Süsens U,Borgmeyer U

    更新日期：2002-12-20 00:00:00

  • The planar cell polarity effector protein Wdpcp (Fritz) controls epithelial cell cortex dynamics via septins and actomyosin.

    abstract：:Planar cell polarity (PCP) signaling controls polarized behaviors in diverse tissues, including the collective cell movements of gastrulation and the planar polarized beating of motile cilia. A major question in PCP signaling concerns the mechanisms linking this signaling cascade with more general cytoskeletal element...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.078

    authors： Park TJ,Kim SK,Wallingford JB

    更新日期：2015-01-09 00:00:00

  • Peptides from the amino-terminus of RANTES cause chemotaxis of human T-lymphocytes.

    abstract：:RANTES is a potent chemotactic and activating agent for a variety of leukocytes including T-lymphocytes. To identify the region of the molecule responsible for this activity, we have made overlapping 10 amino acid peptides scanning the protein. A micro-Boyden chamber chemotaxis assay showed the most efficacious peptid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1783

    authors： Wells TN,Guye-Coulin F,Bacon KB

    更新日期：1995-06-06 00:00:00

  • Purification of a novel serine proteinase inhibitor from the skeletal muscle of white croaker (Argyrosomus argentatus).

    abstract：:A novel serine proteinase inhibitor has been purified to homogeneity from the skeletal muscle of white croaker (Argyrosomus argentatus). The purification was carried out by ammonium sulfate fractionation, DEAE-Sephacel, heating treatment followed by column chromatographies on SP-Sepharose, Sephadex G-150 and gel-filtr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2803

    authors： Cao MJ,Osatomi K,Matsuda R,Ohkubo M,Hara K,Ishihara T

    更新日期：2000-06-07 00:00:00

  • Analysis of recombinant human saposin A expressed by Pichia pastoris.

    abstract：:Saposins (SAPs) are small glycoproteins required for activation of sphingolipid hydrolysis by lysosomal enzymes. Four SAPs, SAP-A, -B, -C, and -D, are proteolytically cleaved from a single gene product termed prosaposin. The mature coding sequence of human SAP-A tagged with 6-histidine was expressed in Pichia pastoris...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.069

    authors： Yamada M,Inui K,Hamada D,Nakahira K,Yanagihara K,Sakai N,Nishigaki T,Ozono K,Yanagihara I

    更新日期：2004-05-28 00:00:00

  • A novel role for PTK2B in cultured beige adipocyte differentiation.

    abstract：:Adipocyte differentiation is a tightly regulated process which requires the sequential and organized expression of numerous genes and proteins. Phosphorylation of cytoplasmic proteins and key transcription factors represents a critical regulatory mechanism of the process leading to adipocyte maturation and modulation ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.021

    authors： Ni B,Farrar JS,Chen S,Lownik JC,Celi FS

    更新日期：2018-07-02 00:00:00

  • Cell cycle effect on the activity of deoxynucleoside analogue metabolising enzymes.

    abstract：:Deoxynucleoside analogues (dNAs) are cytotoxic towards both replicating and indolent malignancies. The impact of fluctuations in the metabolism of dNAs in relation to cell cycle could have strong implications regarding the activity of dNAs. Deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK) are important enzym...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.03.176

    authors： Fyrberg A,Albertioni F,Lotfi K

    更新日期：2007-06-15 00:00:00

  • Evaluation of the reliability of the maximum entropy method for reconstructing 3D and 4D NOESY-type NMR spectra of proteins.

    abstract：:Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and limited digital resolution. Recently, new acquisition techniques for speeding up multidimensional NMR experiments, such as nonlinear sampling, i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.12.088

    authors： Shigemitsu Y,Ikeya T,Yamamoto A,Tsuchie Y,Mishima M,Smith BO,Güntert P,Ito Y

    更新日期：2015-02-06 00:00:00

  • POU-domain genes in planarian Dugesia japonica: the structure and expression.

    abstract：:In fresh water planarian Dugesia japonica, two genes encoding POU-domain proteins, DjPOU1 and DjPOU2, have been identified by polymerase chain reaction (PCR). Their POU-domains are strikingly similar to class III POU-domains in mammals, frog, insects and nematode. The full-length cDNA of DjPOU1 has been cloned and seq...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1571

    authors： Orii H,Agata K,Watanabe K

    更新日期：1993-05-14 00:00:00

  • GTP-binding membrane proteins in activated and differentiating T cells.

    abstract：:We have earlier reported changes in the GTP binding of several membrane proteins including Gs alpha and Gi alpha during thymic differentiation of T cells. Using an [alpha-32P]GTP-photoaffinity labeling technique we have studied the pattern of GTP binding proteins in activated and resting T lymphocytes and in T cells i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91790-j

    authors： Pessa-Morikawa T,Nordström T,Mustelin T,Andersson LC

    更新日期：1991-07-15 00:00:00

  • Hyaluronic acid is an endogenous inducer of interleukin-1 production by human monocytes and rabbit macrophages.

    abstract：:When human peripheral monocytes and rabbit peritoneal macrophages were incubated with hyaluronic acid, the media were found to contain interleukin-1 (IL-1) activity and to stimulate collagenase production by rabbit fibroblasts. A digestion of hyaluronic acid by testicular hyaluronidase decreased the IL-1 inducing acti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90790-4

    authors： Hiro D,Ito A,Matsuta K,Mori Y

    更新日期：1986-10-30 00:00:00

  • IL-6 activates HSP72 gene expression in human skeletal muscle.

    abstract：:To determine whether the cytokine interleukin (IL)-6 induces heat shock protein (HSP) 72 gene expression in skeletal muscle, 18 healthy, young men had either a high dose of IL-6 (HiIL-6; n=6), low dose IL-6 (LoIL-6; n=6), or saline (CON; n=6) infused into one femoral artery for 3h. Muscle biopsies were obtained from t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0006-291x(02)02079-x

    authors： Febbraio MA,Steensberg A,Fischer CP,Keller C,Hiscock N,Pedersen BK

    更新日期：2002-09-06 00:00:00

  • A new function of Ito cells in liver morphogenesis: evidence using a novel morphogenic protein, epimorphin, in vitro.

    abstract：:A novel mesenchymal protein, epimorphin, has been reported to play a key role in morphogenesis of fetal organs. The morphological and functional features of epimorphin in adult liver were entirely unknown. This study was performed to determine the distribution and function of this protein in the adult mouse liver. Epi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.1146

    authors： Hirose M,Watanabe S,Oide H,Kitamura T,Miyazaki A,Sato N

    更新日期：1996-08-05 00:00:00

  • Thiol-containing molecules interact with the myeloperoxidase/H2O2/chloride system to inhibit LDL oxidation.

    abstract：:Oxidized low-density lipoproteins (LDL) accumulate in the vascular wall and promote a local inflammatory process contributing to the progression of atheromatous plaque. The key role of myeloperoxidase (MPO) in this process has been documented and the enzyme has been involved in the oxidative modification of apolipopro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.09.013

    authors： Van Antwerpen P,Boudjeltia KZ,Babar S,Legssyer I,Moreau P,Moguilevsky N,Vanhaeverbeek M,Ducobu J,Nève J

    更新日期：2005-11-11 00:00:00

  • Differences in stability of recombinant apoaequorin within subcellular compartments.

    abstract：:Recombinant acquorin is widely used as an intracellular Ca2+ indicator within live cells. Our data shows that recombinant apoacquorin was unstable within the cytosol, with a half life of approximately 20 minutes. Targeting of the protein to subcellular organelles resulted in an increase in stability which may be due t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2862

    authors： Badminton MN,Sala-Newby GB,Kendall JM,Campbell AK

    更新日期：1995-12-26 00:00:00

  • Characterisation of two novel CYP4 genes from the marine polychaete Nereis virens and their involvement in pyrene hydroxylase activity.

    abstract：:Cytochrome P450 enzymes (CYP enzymes) catalyse the initial step in biotransformation of xenobiotics like polycyclic aromatic hydrocarbons (PAHs). The marine polychaete Nereis virens has a high capacity for biotransformation of PAHs. In the present study, the complete cDNA sequences of two novel CYP genes isolated from...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.189

    authors： Jørgensen A,Rasmussen LJ,Andersen O

    更新日期：2005-10-28 00:00:00

  • Identification and functional analysis of candidate gene VPS28 for milk fat in bovine mammary epithelial cells.

    abstract：:In a previous genome-wide association study on milk production traits in Chinese Holstein population, we revealed VPS28 gene was highly expressed in mammary gland tissue and a -58C > T mutation in 5'-UTR of it was significantly associated with milk fat content traits. In this study, we explored the effect of this -58C...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.01.016

    authors： Liu L,Zhang Q

    更新日期：2019-03-19 00:00:00

  • Heat shock protein 27 in neuronal survival and neurite outgrowth.

    abstract：:The small heat shock protein 27 (Hsp27) is well documented to promote neuronal survival in neurodegenerative diseases and following nerve injury. It can directly inhibit apoptotic pathways, and as a chaperone it can ameliorate the toxic effects of misfolded proteins. More recently, Hsp27 has been implicated to also pl...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2009.02.114

    authors： Read DE,Gorman AM

    更新日期：2009-04-24 00:00:00

  • Reprogramming of cancer stem cells into non-tumorigenic cells using stem cell exosomes for cancer therapy.

    abstract：:Cancer stem cells (CSCs) are a small population of cells with stem cell-like properties found in tumors. CSCs are closely associated with tumor heterogeneity, which influences tumor progress, metastasis, and drug resistance. Here, we propose a concept to enhance efficacy of cancer therapy through CSC reprogramming int...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.072

    authors： Lee KS,Choi JS,Cho YW

    更新日期：2019-05-07 00:00:00

  • Expression of the C-terminal domain of novel human SR-A1 protein: interaction with the CTD domain of RNA polymerase II.

    abstract：:We have recently cloned a new member of the human Ser/Arg-rich superfamily (SR) of pre-mRNA splicing factors, SR-A1. Members of the SR family of proteins have been shown to interact with the C-terminal domain (CTD) of the large subunit of RNA polymerase II, and participate in pre-mRNA splicing. The largest subunit of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.06.053

    authors： Katsarou ME,Papakyriakou A,Katsaros N,Scorilas A

    更新日期：2005-08-19 00:00:00

  • A rapid and sensitive PCR screening method for point mutations associated with mitochondrial encephalomyopathies.

    abstract：:Alterations of the mitochondrial DNA, encoding important parts of the cellular energy-generating system (oxidative phosphorylation, OXPHOS), are often associated with the occurrence of degenerative neuromuscular diseases. Especially point mutations in the mitochondrial tRNA genes, which cannot be complemented by the n...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1540

    authors： Seibel P,Flierl A,Kottlors M,Reichmann H

    更新日期：1994-04-29 00:00:00

  • Erybraedin A is a potential Src inhibitor that blocks the adhesion and viability of non-small-cell lung cancer cells.

    abstract：:The adhesion of cancer cells to the extracellular matrix (ECM) is crucial for cell proliferation, survival, and metastasis. Thus, it is necessary to inhibit cell-ECM adhesion by blocking the activation of the associated signaling to control cancer. Here, we identify erybraedin A (EBA) as a potential Src inhibitor that...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.05.137

    authors： Min HY,Jung Y,Park KH,Oh WK,Lee HY

    更新日期：2018-07-07 00:00:00

  • A direct evidence of the localization of mitochondrial calmodulin.

    abstract：:The presence and localization of mitochondrial calmodulin was directly proved immuno-electron microscopically by the protein A-gold technique. In the ultra-pure mitochondria the complexes of anti-calmodulin antibody and protein A-gold clearly showed the localization of mitochondrial calmodulin on the inner membrane an...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90988-x

    authors： Hatase O,Doi A,Itano T,Matsui H,Ohmura Y

    更新日期：1985-10-15 00:00:00

  • Mechanisms of sterol regulatory element-binding protein-2 (SREBP-2) regulation of human prostasin gene expression.

    abstract：:Prostasin is a glycosylphosphatidylinositol (GPI)-anchored serine protease and a suppressor of tumor cell invasion. We recently reported that the human prostasin gene is up-regulated by the transcription factor sterol regulatory element-binding protein-2 (SREBP-2). In the present study, we identified multiple SREBP-2 ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.06.049

    authors： Chen M,Chen LM,Chai KX

    更新日期：2006-08-11 00:00:00

  • Characterization of an alveolyn-like protein associated with mouse type 2 pneumocytes using immunochemical methods.

    abstract：:Delipidated proteins from a fraction of mouse lung homogenate enriched for lamellar bodies of type 2 pneumocytes were characterized by a combination of gel filtration and enzyme immunoassay for the presence of a surfactant-associated antigen. Immunoreactivity was associated with a protein of Mr 250-270,000 as well as ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：

    authors： Kumar RK,Smith GJ,Truscott JY,Hristoforidis CP,Lykke AW

    更新日期：1986-09-30 00:00:00

  • The β-domain of cluster 2b streptokinase is a major determinant for the regulation of its plasminogen activation activity by cellular plasminogen receptors.

    abstract：:Cluster 2b streptokinase (SK2b), secreted by invasive skin-trophic strains of Streptococcus pyogenes (GAS), is a human plasminogen (hPg) activator that optimally functions when human plasma hPg is bound, via its kringle-2 domain, to cognizant bacterial cells through the a1a2 domain of the major cellular hPg receptor, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.01.109

    authors： Zhang Y,Mayfield JA,Ploplis VA,Castellino FJ

    更新日期：2014-02-21 00:00:00

  • Implication of mRNA binding proteins in the regulation of cyclo-oxygenase in human amnion at term.

    abstract：:The onset of labour is associated with an increase in prostaglandin synthesis in amnion which appears to be mediated at least in part by an increase in cyclo-oxygenase (COX) expression. We have tested the hypothesis that COX expression is controlled in amnion by the binding of a protein to the COX mRNA which may inhib...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2149

    authors： Slater D,Berger L,Moore G,Bennett P

    更新日期：1994-08-30 00:00:00

  • Involvement of a chromatin modifier in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell injury: probably an indirect action via the regulation of NFκB/FasL circuitry.

    abstract：:The Fas/FasL signaling pathway, controlled by nuclear factor-κB (NFκB) at the transcriptional level, is critical for triggering germ cell apoptosis in response to mono-(2-ethylhexyl) phthalate (MEHP)-induced Sertoli cell (SC) injury, but the exact regulation mechanism remain unknown. Here, we discovered that expressio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.09.135

    authors： Chen S,Dong Y,Xu C,Jiang L,Chen Y,Jiang C,Hou W,Li W

    更新日期：2013-11-01 00:00:00