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Differences in stability of recombinant apoaequorin within subcellular compartments.

Abstract：

:Recombinant acquorin is widely used as an intracellular Ca2+ indicator within live cells. Our data shows that recombinant apoacquorin was unstable within the cytosol, with a half life of approximately 20 minutes. Targeting of the protein to subcellular organelles resulted in an increase in stability which may be due to either stabilisation of the photoprotein structure or the absence of the relevant proteases within the organelles. When the apoprotein was reconstituted with the prosthetic group coelenterazine, there was a substantial increase in it's half life in the cytosol. We propose that this variable stability makes acquorin an ideal reporter of gene expression.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Badminton MN,Sala-Newby GB,Kendall JM,Campbell AK

doi

10.1006/bbrc.1995.2862

subject

Has Abstract

pub_date

1995-12-26 00:00:00

pages

950-7

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006291X85728628

journal_volume

217

pub_type

杂志文章

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