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Analysis of tissue-specific region in sericin 1 gene promoter of Bombyx mori.

Abstract：

:The gene encoding sericin 1 (Ser1) of silkworm (Bombyx mori) is specifically expressed in the middle silk gland cells. To identify element involved in this transcription-dependent spatial restriction, truncation of the 5' terminal from the sericin 1 (Ser1) promoter is studied in vivo. A 209bp DNA sequence upstream of the transcriptional start site (-586 to -378) is found to be responsible for promoting tissue-specific transcription. Analysis of this 209bp region by overlapping deletion studies showed that a 25bp region (-500 to -476) suppresses the ectopic expression of the Ser1 promoter. An unknown factor abundant in fat body nuclear extracts is shown to bind to this 25bp fragment. These results suggest that this 25bp region and the unknown factor are necessary for determining the tissue-specificity of the Ser1 promoter.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Liu Y,Yu L,Guo X,Guo T,Wang S,Lu C

doi

10.1016/j.bbrc.2006.01.140

subject

Has Abstract

pub_date

2006-03-31 00:00:00

pages

273-9

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)00236-1

journal_volume

342

pub_type

杂志文章

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