Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism.


:Very-long-chain acyl-CoA synthetases (VLCS) activate very-long-chain fatty acids (VLCFA) containing 22 or more carbons to their CoA derivatives. We cloned the human ortholog (hVLCS) of the gene encoding the rat liver enzyme (rVLCS). Both hVLCS and rVLCS contain 620 amino acids, are expressed primarily in liver and kidney, and have a potential peroxisome targeting signal 1 (-LKL) at their carboxy termini. When expressed in COS-1 cells, hVLCS activated the VLCFA lignoceric acid (C24:0), a long-chain fatty acid (C16:0), and two branched-chain fatty acids, phytanic acid and pristanic acid. Immunofluorescence and immunoblot studies localized hVLCS to both peroxisomes and endoplasmic reticulum. In peroxisomes of HepG2 cells, hVLCS was topographically oriented facing the matrix and not the cytoplasm. This orientation, coupled with the observation that hVLCS activates branched-chain fatty acids, suggests that hVLCS could play a role in the intraperoxisomal reactivation of pristanic acid produced via alpha-oxidation of phytanic acid.


Steinberg SJ,Wang SJ,Kim DG,Mihalik SJ,Watkins PA




Has Abstract


1999-04-13 00:00:00














  • An N-terminal fragment of mouse DGAT1 binds different acyl-CoAs with varying affinity.

    abstract::A histidine-tagged recombinant N-terminal fragment of type-1 mouse liver diacylglycerol acyltransferase (DGAT; EC, MmDGAT1(1-95)His6, was expressed in Escherichia coli, and used to investigate possible acyl-CoA-binding properties. Analysis of the purified fragment by MALDI-TOF mass spectrometry revealed a po...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Siloto RM,Madhavji M,Wiehler WB,Burton TL,Boora PS,Laroche A,Weselake RJ

    更新日期:2008-08-29 00:00:00

  • Comparative proteomics analysis of human lung squamous carcinoma.

    abstract::Two-dimensional polyacrylamide gel electrophoresis (2-DE) profiles of human lung squamous carcinoma tissue and paired surrounding normal bronchial epithelial tissue were compared. Selected differential protein-spots were identified with peptide mass fingerprinting based on matrix-assisted laser desorption/ionization t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Li C,Chen Z,Xiao Z,Wu X,Zhan X,Zhang X,Li M,Li J,Feng X,Liang S,Chen P,Xie JY

    更新日期:2003-09-12 00:00:00

  • Inhibition of Na,K-ATPase activates PI3 kinase and inhibits apoptosis in LLC-PK1 cells.

    abstract::In the present study we used LLC-PK1 cells, a porcine renal proximal tubular cell line, to investigate whether PI3 kinase activation was involved in the anti-apoptotic effect of ouabain, a specific inhibitor of Na,K-ATPase. Apoptosis was induced by actinomycin D (Act D, 5 microM) and assessed by appearance of hypodipl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhou X,Jiang G,Zhao A,Bondeva T,Hirszel P,Balla T

    更新日期:2001-07-06 00:00:00

  • FAD oxidizes the ERO1-PDI electron transfer chain: the role of membrane integrity.

    abstract::The molecular steps of the electron transfer in the endoplasmic reticulum from the secreted proteins during their oxidation are relatively unknown. We present here that flavine adenine dinucleotide (FAD) is a powerful oxidizer of the oxidoreductase system, Ero1 and PDI, besides the proteins of rat liver microsomes and...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Papp E,Nardai G,Mandl J,Bánhegyi G,Csermely P

    更新日期:2005-12-16 00:00:00

  • Enhanced expression and autoimmunity of recombination signal binding protein-jkappa in human dilated cardiomyopathy.

    abstract::Dilated cardiomyopathy (DCM) is a major cause of heart failure in younger individuals. Its prognosis is poor with 40-50% of patients dying within 2 years after diagnosis. Although the etiology of DCM is poorly understood, there is increasing evidence that DCM may represent an autoimmune disease in a significant subset...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nickenig G,Wolff M,Stäblein A,Pfister H,Böhm M

    更新日期:1999-12-20 00:00:00

  • Depression of MAD2 inhibits apoptosis of gastric cancer cells by upregulating Bcl-2 and interfering mitochondrion pathway.

    abstract::Mitotic arrest deficient 2 (MAD2) is an essential component of the mitotic spindle checkpoint pathway. It was previously shown to be associated with drug resistance of tumor cells. To further explore the roles of MAD2 in responses of gastric cancer cells to chemotherapy drugs, we constructed the siRNA vectors of MAD2 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Du Y,Yin F,Liu C,Hu S,Wang J,Xie H,Hong L,Fan D

    更新日期:2006-07-07 00:00:00

  • Cloning and sequencing of human LH/hCG receptor cDNA.

    abstract::We have isolated and sequenced a cDNA encoding the human luteinizing hormone--choriogonadotropin (LH/hCG) receptor. The deduced amino acid sequence (699 residues) containing seven putative transmembrane segments displays sequence similarity to G protein-coupled receptors. The receptor consists of 335 residue extracell...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Minegishi T,Nakamura K,Takakura Y,Miyamoto K,Hasegawa Y,Ibuki Y,Igarashi M,Minegish T corrected to Minegishi T

    更新日期:1990-11-15 00:00:00

  • Expression profile analysis of aorta-gonad-mesonephros region-derived stromal cells reveals genes that regulate hematopoiesis.

    abstract::The aorta-gonad-mesonephros (AGM) region is involved in the generation and maintenance of the first definitive hematopoietic stem cells (HSCs). A mouse AGM-derived cell line, AGM-S3, was shown to support the development of HSCs. To elucidate the molecular mechanisms regulating early hematopoiesis, we obtained subclone...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nagao K,Ohta T,Hinohara A,Tahara T,Hagiwara T,Maeda Y,Yoneya T,Sohma Y,Heike T,Nakahata T,Inagaki Y,Nishikawa M

    更新日期:2008-12-05 00:00:00

  • Ferulenol specifically inhibits succinate ubiquinone reductase at the level of the ubiquinone cycle.

    abstract::The natural compound ferulenol, a sesquiterpene prenylated coumarin derivative, was purified from Ferula vesceritensis and its mitochondrial effects were studied. Ferulenol caused inhibition of oxidative phoshorylation. At low concentrations, ferulenol inhibited ATP synthesis by inhibition of the adenine nucleotide tr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lahouel M,Zini R,Zellagui A,Rhouati S,Carrupt PA,Morin D

    更新日期:2007-03-30 00:00:00

  • The expression of inhibin beta B is high in human adipocytes, reduced by weight loss, and correlates to factors implicated in metabolic disease.

    abstract::Adipose tissue is an endocrine organ that produces and secretes adipokines. The aim of this study was to identify genes predominantly expressed in human subcutaneous adipocytes. For this purpose, an algorithm was developed and DNA microarray expression profiles from 33 human tissues and cell types were used to select ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sjöholm K,Palming J,Lystig TC,Jennische E,Woodruff TK,Carlsson B,Carlsson LM

    更新日期:2006-06-16 00:00:00

  • Calmodulin mutant in central linker reduces the binding affinity with PreIQ and IQ while interacting with CaV1.2 channels.

    abstract::Calmodulin (CaM) was reported to interact with PreIQ and IQ of CaV1.2 channels, but to date, no explicit binding sites of CaM were illustrated. Therefore, in the present study, we firstly used MOE (Molecular Operating Environment) for protein-protein docking and we found that the most likely residues of CaM that play ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu Y,Yan S,Chen S,Zhang J,Shen Y,Su J,He G,Feng R,Shao D,Hao L

    更新日期:2020-05-21 00:00:00

  • Hepatocyte growth factor induces mitogenic reaction to the rabbit gastric epithelial cells in primary culture.

    abstract::Hepatocyte Growth Factor (HGF) was originally identified as hepatotrophic factor for liver regeneration. More recently, HGF was revealed to stimulate proliferation of various epithelial cells. At present study, we have investigated the mitogenic effect of HGF on gastric epithelial cells in the primary culture system. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Takahashi M,Ota S,Terano A,Yoshiura K,Matsumura M,Niwa Y,Kawabe T,Nakamura T,Omata M

    更新日期:1993-03-15 00:00:00

  • The octamer motif present in the Rex-1 promoter binds Oct-1 and Oct-3 expressed by EC cells and ES cells.

    abstract::Rex-1 is a zinc finger-containing gene that is expressed in embryonal carcinoma (EC) cells and embryonic stem (ES) cells. Upon differentiation with retinoic acid (RA), transcription of the Rex-1 gene decreases rapidly. Analysis of the 5'-flanking region of the Rex-1 gene identified a consensus motif for the octamer fa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Rosfjord E,Rizzino A

    更新日期:1994-09-30 00:00:00

  • Multiple regulatory elements and binding proteins of the 5'-flanking region of the human estrogen-responsive finger protein (efp) gene.

    abstract::The efp, a member of the RING finger family, was previously identified as an estrogen responsive gene. Here, we characterized basal promoter of the human efp gene. Transcription initiation site was found at position -60 G relative to the site for translation initiation, and TATA motif was absent. Deletion and mutation...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ikeda K,Inoue S,Orimo A,Sano M,Watanabe T,Tsutsumi K,Muramatsu M

    更新日期:1997-07-30 00:00:00

  • Phenobarbital enhances 2',5'-oligoadenylate synthesis in rat liver nuclei.

    abstract::Treatment of rats with phenobarbital for three days greatly increases the activity of 2,5 oligoadenylate synthetase in liver nuclei. Analysis of 2',5'-oligoadenylates synthesized in vitro showed that nuclei from both phenobarbital-treated and control rats synthesized 2',5'-oligoadenylates ranging from di- to hexamers....

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu DK,Owens GF

    更新日期:1984-06-29 00:00:00

  • Biocatalysts: beautiful creatures.

    abstract::The chemical industry has come under increasing pressure to make chemical production more eco-friendly and independent to fossil resources. The development of industrial processes based on micro-organisms can especially help to eliminate the use or the generation of hazardous substances and can support the transition ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Saibi W,Abdeljalil S,Masmoudi K,Gargouri A

    更新日期:2012-09-28 00:00:00

  • RNA polymerase II transcribes Dictyostelium untranslatable gene, dutA, specifically in the developmental phase.

    abstract::In Dictyostelium discoideum, a novel type of RNA (dutA RNA), which is untranslatable, cytosolic and 1.3 kb in size, appears specifically after the aggregation stage [Yoshida, H. et al. (1994) Nucleic Acids Res. 22, 41-46]. We here show that the dutA gene is transcribed by RNA polymerase II, based on its alpha-amanitin...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kumimoto H,Yoshida H,Okamoto K

    更新日期:1995-11-02 00:00:00

  • Activation of prothrombin by two subtilisin-like serine proteases from Acremonium sp.

    abstract::Two novel subtilisin-like serine proteases (AS-E1 and -E2) that activate prothrombin have been identified in a culture of the fungus Acremonium sp. The enzymes were purified through repeated hydrophobic interaction chromatography. The N-terminal sequences of AS-E1 (34.4 kDa) and AS-E2 (32 kDa) showed high similarity t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liu C,Matsushita Y,Shimizu K,Makimura K,Hasumi K

    更新日期:2007-06-22 00:00:00

  • The mosquito larvicidal activity of 130 kDa delta-endotoxin of Bacillus thuringiensis var. israelensis resides in the 72 kDa amino-terminal fragment.

    abstract::Bacillus thuringiensis var. israelensis produces 130 kDa delta-endotoxin which is highly toxic to mosquito-larvae. The mosquito-larvicidal activity was delineated by sequential deletions from ends of the 1136 amino acids delta-endotoxin. A maximum of 459 amino acids could be removed from the carboxy-terminal of the to...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pao-intara M,Angsuthanasombat C,Panyim S

    更新日期:1988-05-31 00:00:00

  • Distinct atrial natriuretic factor receptor sites on cultured bovine aortic smooth muscle and endothelial cells.

    abstract::Cultured bovine aortic smooth muscle and endothelial cells each display distinct specific binding sites for radiolabeled atrial natriuretic peptide (ANF). 125I-pro-rANF (103-126)I binding to both cell types is rapid, reversible and competitive. Scatchard plots of the binding data show Bmax values of 5.5 and 0.1 - 2.1 ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Schenk DB,Johnson LK,Schwartz K,Sista H,Scarborough RM,Lewicki JA

    更新日期:1985-03-15 00:00:00

  • Adaptation to phosphate depletion in opossum kidney cells.

    abstract::Dietary phosphate restriction produces an adaptive increase in renal tubular Na/Pi cotransport. A similar adaptation occurs during phosphate depletion in opossum kidney cells, a continuous line of cultured renal epithelial cells. We investigated the cellular changes associated with adaptation to phosphate depletion in...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Saxena S,Dansby L,Allon M

    更新日期:1995-11-02 00:00:00

  • Endothelin-1 is produced and secreted by neonatal rat cardiac myocytes in vitro.

    abstract::Production of endothelin-1 in the cultured neonatal rat cardiac myocytes was investigated. Studies on the cultured cardiac myocytes with reverse transcription-polymerase chain reaction followed by Southern hybridization indicated that mRNA of ET-1(preproET-1 mRNA) was expressed by cardiac myocytes. ET-1 polypeptide wa...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Suzuki T,Kumazaki T,Mitsui Y

    更新日期:1993-03-31 00:00:00

  • Osteoclast resorption-stimulating activity is associated with the osteoblast cell surface and/or the extracellular matrix.

    abstract::Osteoblasts mediate much of the hormonal responsiveness of osteoclasts. We and others have found that one mechanism through which this regulation is effected is by release of osteoclast resorption-stimulating activity (ORSA) into culture supernatants. However, although hormonal responsiveness is regularly observed in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Fuller K,Gallagher AC,Chambers TJ

    更新日期:1991-11-27 00:00:00

  • Detection of antibody to hepatitis C E2/NS1 protein in patients with type C hepatitis.

    abstract::Putative E2/NS1 sequence of hepatitis C virus was expressed in E. coli as a fusion protein with maltose binding protein. Approximately 80 kDa protein was obtained containing 38 kDa E2/NS1 protein. The antibody to this protein was detectable in the same serum from which the sequence was amplified. It was also detectabl...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yokosuka O,Ito Y,Imazeki F,Ohto M,Omata M

    更新日期:1992-11-30 00:00:00

  • Involvement of proton-sensing receptor TDAG8 in the anti-inflammatory actions of dexamethasone in peritoneal macrophages.

    abstract::Dexamethasone (DEX), a potent glucocorticoid, increased the expression of T-cell death associated gene 8 (TDAG8), a proton-sensing G protein-coupled receptor, which is associated with the enhancement of acidic pH-induced cAMP accumulation, in peritoneal macrophages. We explored the role of increased TDAG8 expression i...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: He XD,Tobo M,Mogi C,Nakakura T,Komachi M,Murata N,Takano M,Tomura H,Sato K,Okajima F

    更新日期:2011-12-02 00:00:00

  • Combination of 3-methyladenine therapy and Asn-Gly-Arg (NGR)-modified mesoporous silica nanoparticles loaded with temozolomide for glioma therapy in vitro.

    abstract::Mesoporous silica nanoparticles (MSNPs) of a small diameter were loaded with the anticancer drug temozolomide (TMZ), coated with polydopamine (PDA), and conjugated with Asn-Gly-Arg (NGR) for use in the treatment of glioma. The accumulation of NGR-MSNPs in C6 cells was shown to be higher than that of unmodified MSNPs. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang P,Tang M,Huang Q,Zhao G,Huang N,Zhang X,Tan Y,Cheng Y

    更新日期:2019-02-05 00:00:00

  • Copper induces structural changes in N-terminus of human prion protein.

    abstract::Copper ions reportedly bind to the cellular prion (PrPC) and induce PrP proteinase K (PK) resistant from (PrPres). PrPC also plays a role in response to oxidative stress. By using purified human PrP23-98 containing octarepeats, we have found that Cu(II) induces PrPres determined by Western blots and atomic force micro...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lu B,Zhao L,Qin K

    更新日期:2018-05-15 00:00:00

  • Cholecystokinin-induced redistribution of paxillin in rat pancreatic acinar cells.

    abstract::Cholecystokinin (CCK) dose-dependently stimulates enzyme secretion or loss of cell integrity in the exocrine pancreas. Signaling mechanims include tyrosine phosphorylation of p125(FAK) and paxillin. Here, we examine their potential function. Maximum phosphorylation of both proteins was observed after stimulation of fr...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Leser J,Lührs H,Beil MF,Adler G,Lutz MP

    更新日期:1999-01-19 00:00:00

  • Differential caspase activity in the cortex and striatum with chronic infusion of 3-nitropropionic acid.

    abstract::Systemic administration of 3-nitropropionic acid (3-NP) facilitates the development of select striatal lesions, and some reports provide clues about this pathology. In this study, we investigated the relationship between reduced levels of brain-derived neurotrophic factor (BDNF) in lesioned brain regions and caspase a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Cho KJ,Kim GW

    更新日期:2015-09-25 00:00:00

  • The vWFA2 domain of type VII collagen is responsible for collagen binding.

    abstract::Type VII collagen (Col7) is the major component of anchoring fibrils and very important for skin integrity. This is emphasized by the Col7 related skin blistering diseases dystrophic epidermolysis bullosa and epidermolysis bullosa acquisita. Structural data that provides insights into the interaction network of Col7 a...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wegener H,Leineweber S,Seeger K

    更新日期:2013-01-11 00:00:00