Histone deacetylase 3 (HDAC3) is recruited to target promoters by PML-RARalpha as a component of the N-CoR co-repressor complex to repress transcription in vivo.


:PML-RARalpha is a chimeric transcription factor tightly associated with acute promyelocytic leukemia. PML-RARalpha plays an important role in the aberrant transcription repression on the target genes of wild-type retinoic acid receptors. Here, we demonstrated that HDAC3, one component of the N-CoR transcription repressor complex, is a key regulator of the transcription repression by PML-RARalphain vivo. Using immunoprecipitation, we demonstrated that PML-RARalpha interacts with N-CoR/HDAC3 in vivo without ligand. Next, using chromatin immunoprecipitation (ChIP) assay, this N-CoR/HDAC3 co-repressor complex was recruited to the endogenous target promoters (RARbeta and CYP26) through PML-RARalpha. The neighboring histones were de-acetylated and gene expression was repressed. When HDAC3 protein was knocked down by RNA interference in PML-RARalpha-expressing cells, the endogenous target genes were significantly activated, which was also confirmed by promoter-luciferase reporter assay. These results provide evidence to show that the N-CoR/HDAC3 co-repressor complex is involved in the aberrant transcription regulation in PML-RARalpha-expressing cells.


Atsumi A,Tomita A,Kiyoi H,Naoe T




Has Abstract


2006-07-14 00:00:00














  • Active fragments and analogs of the insect neuropeptide leucopyrokinin: structure-function studies.

    abstract::Evaluation of analogs of the blocked insect myotropic neuropeptide leucopyrokinin (LPK) has demonstrated its relative insensitivity to amino acid substitution in the N-terminal in contrast to the C-terminal region. Truncated analogs of LPK without the first, second, and third N-terminal amino acids retain a significan...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Nachman RJ,Holman GM,Cook BJ

    更新日期:1986-06-30 00:00:00

  • Bacterial overexpression, purification, and reconstitution of the carnitine/acylcarnitine carrier from rat liver mitochondria.

    abstract::The carnitine/acylcarnitine carrier from rat liver mitochondria was overexpressed in Escherichia coli. The expressed protein, recovered as inclusion bodies, was solubilized with sarkosyl and purified by Sephadex G-200 and celite chromatography. A yield of 15 mg of purified transport protein per liter of cell culture w...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Indiveri C,Iacobazzi V,Giangregorio N,Palmieri F

    更新日期:1998-08-28 00:00:00

  • Identification and characterization of a novel gene EOLA1 stimulating ECV304 cell proliferation.

    abstract::To study the changes in gene expression in endothelial cells stimulated by lipopolysaccharide (LPS) we performed subtraction hybridization on control human umbilical vein endothelial cells (HUVEC) versus HUVEC stimulated by LPS. A novel cDNA, named endothelial-overexpressed lipopolysaccharide-associated factor 1 (EOLA...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Liang Z,Yang Z

    更新日期:2004-12-17 00:00:00

  • Metformin promotes the osseointegration of titanium implants under osteoporotic conditions by regulating BMSCs autophagy, and osteogenic differentiation.

    abstract::Osteoporosis is a common bone disorder with adverse effects on oral osseointegration, and the effects of metformin on bone metabolism have received increasing attention. The aim of the present study was to test the hypothesis that metformin promoted osteogenesis of bone mesenchymal stem cells (BMSCs) and osseointegrat...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Lin J,Xu R,Shen X,Jiang H,Du S

    更新日期:2020-10-15 00:00:00

  • Both p53-PUMA/NOXA-Bax-mitochondrion and p53-p21cip1 pathways are involved in the CDglyTK-mediated tumor cell suppression.

    abstract::CDglyTK fusion suicide gene has been well characterized to effectively kill tumor cells. However, the exact mechanism and downstream target genes are not fully understood. In our study, we found that CDglyTK/prodrug treatment works more efficiently in p53 wild-type (HONE1) cells than in p53 mutant (CNE1) cells. We the...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Yu Z,Wang H,Zhang L,Tang A,Zhai Q,Wen J,Yao L,Li P

    更新日期:2009-09-04 00:00:00

  • The impact of N-cadherin-β-catenin signaling on the analgesic effects of glial cell-derived neurotrophic factor in neuropathic pain.

    abstract::Long-term neuropathic pain can lead to anxiety, depression, and other issues, which seriously affect patients' quality of life. For this reason, it is important to find effective treatments. Studies have shown that glial cell-derived neurotrophic factor (GDNF) can relieve neuropathic pain. However, its mechanism of ac...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pan Y,Jiang L,Wang C

    更新日期:2020-02-05 00:00:00

  • Rimonabant is a dual inhibitor of acyl CoA:cholesterol acyltransferases 1 and 2.

    abstract::Acyl coenzyme A:cholesterol acyltransferase (ACAT) catalyzes the intracellular synthesis of cholesteryl esters (CE). Both ACAT isoforms, ACAT1 and ACAT2, play key roles in the pathophysiology of atherosclerosis and ACAT inhibition retards atherosclerosis in animal models. Rimonabant, a type 1 cannabinoid receptor (CB1...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Netherland C,Thewke DP

    更新日期:2010-08-06 00:00:00

  • The chimeric gene linked to glucocorticoid-suppressible hyperaldosteronism encodes a fused P-450 protein possessing aldosterone synthase activity.

    abstract::Glucocorticoid-suppressible hyperaldosteronism (GSH) is one variety of primary aldosteronism with hypertension and is inherited in an autosomal dominant mode. A recent report has indicated that GSH is caused by a gene duplication arising from unequal crossing over between the two genes, CYP11B1 and CYP11B2, encoding P...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Miyahara K,Kawamoto T,Mitsuuchi Y,Toda K,Imura H,Gordon RD,Shizuta Y

    更新日期:1992-12-15 00:00:00

  • Non-canonical pathway induced by Wnt3a regulates β-catenin via Pyk2 in differentiating human neural progenitor cells.

    abstract::Wnt/β-catenin and Wnt/Ca2+ pathways are involved in cellular processes during embryonic development and the interaction between them in the same cell decides the outcome of cellular functions. In this study, we showed that Wnt3a triggers the Wnt/Ca2+ signaling pathway, indicated by an increase of cytosolic free calciu...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Narendra Talabattula VA,Morgan P,Frech MJ,Uhrmacher AM,Herchenröder O,Pützer BM,Rolfs A,Luo J

    更新日期:2017-09-09 00:00:00

  • Enzymic and nonenzymic mono ADP-ribosylation of proteins in skeletal muscle.

    abstract::The acceptors of endogenously catalyzed monoADP-ribosylation in the cell free extract from rat skeletal muscle was searched. The main acceptor proteins in particulate were electrophoretically 52, 80, 100, and greater than 200 kDa proteins in the presence of SDS, while that in cytosol were 36 and 39 kDa proteins. Altho...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Tanaka Y,Yoshihara K,Kamiya T

    更新日期:1989-09-15 00:00:00

  • Acute stress enhances learning and memory by activating acid-sensing ion channels in rats.

    abstract::Acute stress has been shown to enhance learning and memory ability, predominantly through the action of corticosteroid stress hormones. However, the valuable targets for promoting learning and memory induced by acute stress and the underlying molecular mechanisms remain unclear. Acid-sensing ion channels (ASICs) play ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ye S,Yang R,Xiong Q,Yang Y,Zhou L,Gong Y,Li C,Ding Z,Ye G,Xiong Z

    更新日期:2018-04-15 00:00:00

  • Effects of oxysterols on the blood-brain barrier: implications for Alzheimer's disease.

    abstract::Altered brain cholesterol homeostasis plays a key role in neurodegenerative diseases such as Alzheimer's disease (AD). For a long time, the blood-brain barrier (BBB) was basically considered as a barrier isolating the brain from circulating cholesterol, however, several lines of evidence now suggest that the BBB stric...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章,评审


    authors: Gosselet F,Saint-Pol J,Fenart L

    更新日期:2014-04-11 00:00:00

  • Impaired IL-2 expression in latent HIV-1 infection.

    abstract::Regarding the T cell function in HIV-1 infection, activation of T cells is enhanced in acutely HIV-1-infected T cells upon stimuli. However, T cell immune responses underlying the activation of T cell receptor (TCR) signaling molecules and interleukin (IL)-2 production in latently HIV-1-infected cells are poorly under...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Shin Y,Yoon CH,Lim H,Park J,Roh TY,Kang C,Choi BS

    更新日期:2015-08-07 00:00:00

  • MAPK and Akt act cooperatively but independently on hypoxia inducible factor-1alpha in rasV12 upregulation of VEGF.

    abstract::Oncogenic ras upregulates the expression of VEGF through the activation of the transcriptional enhancer hypoxia inducible factor-1alpha (HIF-1alpha) by a still poorly understood mechanism. Here, we demonstrate that both the Raf/MEK/MAPK and the PI3 kinase/Akt signaling pathways potently and additively stimulate the ex...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sodhi A,Montaner S,Miyazaki H,Gutkind JS

    更新日期:2001-09-14 00:00:00

  • Isothermal titration calorimetry and vesicle leakage assays highlight the differential behaviors of tau repeat segments upon interaction with anionic lipid membranes.

    abstract::Tau misfolding has been implicated in a variety of tauopathies, including Alzheimer's disease. The microtubule binding domain of tau consists of four repeat segments (R1-R4), and aggregation of these segments leads to the formation of neurofibrillary tangles. Previous studies indicate that misfolded tau associates wit...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Dicke SS,Tatge L,Engen PE,Culp M,Masterson LR

    更新日期:2017-12-02 00:00:00

  • Molecular cloning and characterization of Cup a 4, a new allergen from Cupressus arizonica.

    abstract::Sensitization to Cupressaceae pollen has become one of the most important causes of pollinosis in Western countries during winter and early spring. However, the characterization of the extracts, the allergens involved and the cross-reactivity with other pollen sources still remain poorly studied; in the case of Cupres...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pico de Coaña Y,Parody N,Fuertes MÁ,Carnés J,Roncarolo D,Ariano R,Sastre J,Mistrello G,Alonso C

    更新日期:2010-10-22 00:00:00

  • Lactation suppresses diurnal rhythm of serum leptin.

    abstract::Rats consume most of their daily food intake at night; serum leptin levels and adipose tissue leptin mRNA content are elevated at night in non-lactating rats fed ad libitum. Lactation induces massive hyperphagia with most food still consumed at night, but the nocturnal increase in leptin secretion was not observed in ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pickavance L,Tadayyon M,Williams G,Vernon RG

    更新日期:1998-07-09 00:00:00

  • Inhibition of leukocyte chemotaxis by Glu-Glu-Glu-Glu-Tyr-Pro-Met-Glu and Leu-Ile-Glu-Asp-Asn-Glu-Tyr-Thr-Ala-Arg-Gln-Gly.

    abstract::Chemotaxis of rabbit peritoneal leucocytes stimulated by fMet-Leu-Phe, a synthetic chemoattractant, was inhibited by Glu-Glu-Glu-Glu-Tyr-Pro-Met-Glu (MT peptide) and Leu-Ile-Glu-Asp-Asn-Glu-Tyr-Thr-Ala-Arg-Glu-Gly (Src peptide). Both peptides did not inhibit the binding of [3H] formyl-NLe-Leu-Phe, a chemoattractant, t...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hirata F,Notsu Y,Matsuda K,Vasanthakumar G,Schiffmann E,Wong TW,Goldberg AR

    更新日期:1984-01-30 00:00:00

  • N-and O-acetylation of aromatic and heterocyclic amine carcinogens by human monomorphic and polymorphic acetyltransferases expressed in COS-1 cells.

    abstract::Human monomorphic and polymorphic arylamine acetyltransferases (EC were expressed in monkey kidney COS-1 cells and used to study the N- and O-acetylation of a number of carcinogenic amines and their N-hydroxy metabolites. The monomorphic enzyme N-acetylated the aromatic amines, 2-aminofluorene and 4-aminobiph...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Minchin RF,Reeves PT,Teitel CH,McManus ME,Mojarrabi B,Ilett KF,Kadlubar FF

    更新日期:1992-06-30 00:00:00

  • The heat shock response inhibits inducible nitric oxide synthase gene expression by blocking I kappa-B degradation and NF-kappa B nuclear translocation.

    abstract::We investigated the mechanisms by which the heat shock response inhibits inducible nitric oxide synthase (iNOS) gene expression. Incubation of cultured murine lung epithelium (MLE-15) at temperatures ranging from 39 to 43 degrees C, for 1 h, demonstrated that only severe thermal stress (41 to 43 degrees C) was suffici...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wong HR,Ryan M,Wispé JR

    更新日期:1997-02-13 00:00:00

  • Role of the tumor suppressor RASSF2 in regulation of MST1 kinase activity.

    abstract::The tumor suppressor, RASSF2 (Ras association domain family 2), is frequently downregulated in a number of cancers. Although exogenously expressed RASSF2 induces apoptotic cell death, the precise roles of RASSF2 under pro-apoptotic conditions remain largely unknown. Here, we demonstrate that MST1 (mammalian sterile 20...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Song H,Oh S,Oh HJ,Lim DS

    更新日期:2010-01-01 00:00:00

  • Fibroblast growth factor 3, a protein with a dual subcellular fate, is interacting with human ribosomal protein S2.

    abstract::The secreted isoform of fibroblast growth factor 3 (FGF3) induces a mitogenic cell response, while the nuclear form inhibits cell proliferation. Recently, we identified a nucleolar FGF3-binding protein which is implicated in processing of pre-rRNA as a possible target of nuclear FGF3 signalling. Here, we report a seco...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Antoine M,Reimers K,Wirz W,Gressner AM,Müller R,Kiefer P

    更新日期:2005-12-16 00:00:00

  • Dramatic suppression of colorectal cancer cell growth by the dual mTORC1 and mTORC2 inhibitor AZD-2014.

    abstract::Colorectal cancer is a major contributor of cancer-related mortality. The mammalian target or rapamycin (mTOR) signaling is frequently hyper-activated in colorectal cancers, promoting cancer progression and chemo-resistance. In the current study, we investigated the anti-colorectal cancer effect of a novel mTOR comple...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Huo HZ,Zhou ZY,Wang B,Qin J,Liu WY,Gu Y

    更新日期:2014-01-10 00:00:00

  • Expression and characterization of the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II using the baculovirus expression system.

    abstract::Sf9 cells infected with the recombinant mouse CaMKII-alpha (Ca2+/calmodulin dependent kinase II) baculovirus expressed 12-15 mg of MCaMKII-alpha per liter of cells. Approximately 50% of the MCaMKII-alpha activity could be purified using a CaM-Sepharose affinity column. The purified MCaMKII-alpha had a M(rapp) of 50 kD...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Brickey DA,Colbran RJ,Fong YL,Soderling TR

    更新日期:1990-12-14 00:00:00

  • Biotin enhances ATP synthesis in pancreatic islets of the rat, resulting in reinforcement of glucose-induced insulin secretion.

    abstract::Previous studies showed that biotin enhanced glucose-induced insulin secretion. Changes in the cytosolic ATP/ADP ratio in the pancreatic islets participate in the regulation of insulin secretion by glucose. In the present study we investigated whether biotin regulates the cytosolic ATP/ADP ratio in glucose-stimulated ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sone H,Sasaki Y,Komai M,Toyomizu M,Kagawa Y,Furukawa Y

    更新日期:2004-02-13 00:00:00

  • Model study on the bioreduction of paraquat, MPP+, and analogs. Evidence against a "redox cycling" mechanism in MPTP neurotoxicity.

    abstract::The ability of paraquat, MPP+, and analogs to be reduced by chemical reductants and by NADPH, as catalyzed by liver microsomes or purified NADPH cytochrome P-450 reductase, is reported. The analogs span a range of electrochemical potential, including values in-between that of paraquat and MPP+. Analogs with an Eo belo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Frank DM,Arora PK,Blumer JL,Sayre LM

    更新日期:1987-09-30 00:00:00

  • Loss of preimplantation embryo resulting from a Pum1 gene trap mutation.

    abstract::Pumilio is a member of the highly conserved PUF family of RNA-binding proteins that function as a developmental regulator in diverse animal species. Two Pumilio genes, Pum1 and Pum2, have been identified in mammals and are found to be involved in sperm development, neuron development as well as human diseases such as ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zhang C,Zhu T,Chen Y,Xu EY

    更新日期:2015-06-19 00:00:00

  • Effects of a protein kinase C inhibitor, K-252a, on human polymorphonuclear neutrophil responsiveness.

    abstract::We investigated the capacity of K-252a, an inhibitor of rat brain protein kinase C (PKC), to influence polymorphonuclear neutrophil (PMN) PKC and PMN activation with chemically and structurally dissimilar agonists. K-252a inhibited PMN PKC (IC50 = 0.58 microM), and caused a concentration-dependent (0.1-10 microM) inhi...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Smith RJ,Justen JM,Sam LM

    更新日期:1988-05-16 00:00:00

  • The role of microRNA in the delayed negative feedback regulation of gene expression.

    abstract::Oscillatory gene expression plays an important role in somite segmentation during the early developmental stages of vertebrates. Recent experimental studies have shown that microRNA can regulate gene expression by stimulating degradation of mRNA and/or repression of translation. In this communication, we incorporate m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Xie ZR,Yang HT,Liu WC,Hwang MJ

    更新日期:2007-07-06 00:00:00

  • Lead decreases parasitemia and enhances survival of Plasmodium berghei-infected mice.

    abstract::Malaria, a disease accounting for more than one million deaths per year, is caused by intraerythrocytic growth of Plasmodia. Parasitemia may be blunted by suicidal erythrocyte death or eryptosis, which is characterized by cell shrinkage and phosphatidylserine exposure. Triggers of eryptosis include lead nitrate (Pb(NO...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Koka S,Huber SM,Boini KM,Lang C,Föller M,Lang F

    更新日期:2007-11-23 00:00:00