Requirement of both tyrosine residues 1330 and 1337 in the C-terminal tail of the RON receptor tyrosine kinase for epithelial cell scattering and migration.

abstract：:We have reexamined the intracellular localization of the ubiquitously expressed target membrane SNAP receptor (t-SNARE), SNAP-23. While SNAP-23 appears on the plasma membrane, in the cell types examined there is a significant pool associated with endosomal compartments. Immuno-staining and expression of green fluoresc...

journal_title：Biochemical and biophysical research communications

authors： Chen D,Whiteheart SW

更新日期：1999-02-16 00:00:00

abstract：:Our previous studies have shown that the liver from Naked Mole Rats (NMRs), a long-lived rodent, has increased proteasome activity and lower levels of protein ubiquitination compared to mice. This suggests that protein quality control might play a role in assuring species longevity. To determine whether enhanced prote...

journal_title：Biochemical and biophysical research communications

authors： Pride H,Yu Z,Sunchu B,Mochnick J,Coles A,Zhang Y,Buffenstein R,Hornsby PJ,Austad SN,Pérez VI

更新日期：2015-02-20 00:00:00

abstract：:Osteoprotegerin (OPG) is a member of the TNF receptor superfamily and plays a critical role in the development of osteoclasts from precursor cells. OPG is produced by a variety of cells of mesenchymal origin and has been demonstrated to be present in osteoblasts and osteocytes. However, the mechanisms of regulation of...

journal_title：Biochemical and biophysical research communications

authors： Yang X,Halladay D,Onyia JE,Martin TJ,Chandrasekhar S

更新日期：2002-01-11 00:00:00

abstract：:Chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was expressed in E. coli by using a pET3a T7 RNA polymerase-based expression system and was purified to homogeneity. The kinase and bisphosphatase of the expressed bifunctional enzyme had kinetic properties identical to those of the native chicken live...

journal_title：Biochemical and biophysical research communications

authors： Li L,Yao WZ,Lange AJ,Pilkis SJ,Dong MQ,Yin Y,Xu GJ

更新日期：1995-04-26 00:00:00

abstract：:Invaison plasmid antigen D (IpaD) and water-extracted outer-membrane proteins (OMPs) from Shigella flexneri were used to investigate some of the structural relationships of this pathogen's invasions. Extracellular presentation of the three invasion plasmid antigens (Ipa) B, C, and D is required for the S. flexneri inv...

journal_title：Biochemical and biophysical research communications

authors： Marquart ME,Picking WL,Picking WD

更新日期：1995-09-25 00:00:00

abstract：:Voltage-gated ion channels (VGCs) mediate selective diffusion of ions across cell membranes to enable many vital cellular processes. Three-dimensional structure data are lacking for VGC proteins; hence, to better understand their function, there is a need to identify the conserved motifs using sequence analysis method...

journal_title：Biochemical and biophysical research communications

authors： Guda P,Bourne PE,Guda C

更新日期：2007-01-12 00:00:00

abstract：:Resolvin E1 (RvE1; 5S, 12R, 18R-trihydroxyeicosapentaenoic acid) is an anti-inflammatory lipid mediator derived from the omega-3 fatty acid eicosapentaenoic acid (EPA). It has been recently shown that RvE1 is involved in the resolution of inflammation. However, it is not known whether RvE1 is involved in the resolutio...

journal_title：Biochemical and biophysical research communications

authors： Aoki H,Hisada T,Ishizuka T,Utsugi M,Kawata T,Shimizu Y,Okajima F,Dobashi K,Mori M

更新日期：2008-03-07 00:00:00

abstract：:Due to the structural and functional importance of tight turns, some methods have been proposed to predict gamma-turns, beta-turns, and alpha-turns in proteins. In the past, studies of pi-turns were made, but not a single prediction approach has been developed so far. It will be useful to develop a method for identify...

journal_title：Biochemical and biophysical research communications

authors： Wang Y,Xue ZD,Shi XH,Xu J

更新日期：2006-09-01 00:00:00

abstract：:Incorporation of [3H]-thymidine into DNA in non-synchronized cultures of human endothelial cells was blocked by a 24 h exposure to TNF in a dose dependent manner that resulted in accumulation of cells in G1, as assayed by flow cytometry analysis of DNA content. Proliferation restarted when cells were replated in the a...

journal_title：Biochemical and biophysical research communications

authors： López-Marure R,Bernal AE,Zentella A

更新日期：1997-07-30 00:00:00

abstract：:Microfilaments play an important role in the formation of apoptotic bodies. This study examined expression patterns of beta-actin, a key component of microfilament, during apoptosis. The data suggest that continuous beta-actin expression is not required for formation of apoptotic bodies. beta-actin mRNA levels rapidly...

journal_title：Biochemical and biophysical research communications

authors： Naora H,Naora H

更新日期：1995-06-15 00:00:00

abstract：:Abscisic acid (ABA) is an important plant hormone that mediates abiotic stresses in plant growth and development. A number of E3 ligases have been reported to be involved in ABA signaling pathway. In this study, we identified a C3H2C3 RING-type E3 ligase, Arabidopsis thaliana Tόxicos en Levadura 61 (ATL61), which regu...

journal_title：Biochemical and biophysical research communications

authors： Yang R,Wang T,Shi W,Li S,Liu Z,Wang J,Yang Y

更新日期：2020-07-23 00:00:00

abstract：:The intracellular parasitic protist Trypanosoma cruzi is the causative agent of Chagas disease in Latin America. In general, pyrimidine nucleotides are supplied by both de novo biosynthesis and salvage pathways. While epimastigotes-an insect form-possess both activities, amastigotes-an intracellular replicating form o...

journal_title：Biochemical and biophysical research communications

authors： Hashimoto M,Morales J,Fukai Y,Suzuki S,Takamiya S,Tsubouchi A,Inoue S,Inoue M,Kita K,Harada S,Tanaka A,Aoki T,Nara T

更新日期：2012-01-20 00:00:00

abstract：:Although the Vpr protein of human immunodeficiency virus type 1 (HIV-1) has been shown to act as a transcriptional activator of the HIV-1 LTR and certain host genes, the current study demonstrates that it can also function as a potent inhibitor of the cytomegalovirus (CMV) promoter. Previous studies have shown that th...

journal_title：Biochemical and biophysical research communications

authors： Liu X,Guo H,Wang H,Markham R,Wei W,Yu XF

更新日期：2015-04-03 00:00:00

abstract：:The human amyloid beta protein (BP) is a major constituent of the amyloid deposited in the brain of patients with Alzheimer's disease and is derived from a larger precursor protein (BPP). In human three alternatively-spliced forms of BPP mRNA were found and two of them were shown to encode a protease inhibitory activi...

journal_title：Biochemical and biophysical research communications

authors： Yamada T,Sasaki H,Dohura K,Goto I,Sakaki Y

更新日期：1989-02-15 00:00:00

abstract：:Mammalian homologues of the Drosophila trp gene have been invoked as the structural basis for the currents associated with capacitative Ca2+ entry (CCE) in many cell types. Trp homologues are members of a large protein family that may associate as channel subunits providing an explanation for the functional diversity ...

journal_title：Biochemical and biophysical research communications

authors： Garcia RL,Schilling WP

更新日期：1997-10-09 00:00:00

abstract：:Treatment of platelet rich plasma (PRP) at pH 5 results in the precipitation of a protein kinase system. The protein kinase is associated with the platelet fraction and is capable of phosphorylation of several plasma proteins. Analysis of the 32P-labeled phosphoproteins by two dimensional gel electrophoresis showed th...

journal_title：Biochemical and biophysical research communications

authors： Galabru J,Krust B,Hovanessian AG

更新日期：1983-06-15 00:00:00

abstract：:Glycerol-3-phosphate acyltransferase, mitochondrial (GPAM) is a rate-limiting enzyme catalyzing triglyceride synthesis. Recently, we demonstrated that the anti-viral drug ribavirin (RBV) reduces GPAM expression by downregulating CCAAT/enhancer-binding protein α (C/EBPα). However, the precise mechanisms of GPAM suppres...

journal_title：Biochemical and biophysical research communications

authors： Onomura D,Satoh S,Ueda Y,Dansako H,Kato N

更新日期：2020-11-26 00:00:00

abstract：:Force impulse is thought to be generated in muscle when myosin head (S-1), while weakly bound to actin filament, undergoes orientational change to form a strong (rigor) bond with actin. There is ample evidence that this bond involves interaction of 1 myosin head with 1 actin monomer. However, X-ray diffraction data of...

journal_title：Biochemical and biophysical research communications

authors： Andreev OA,Borejdo J

更新日期：1991-05-31 00:00:00

abstract：:Through the proteomic analysis using 2-dimensional electrophoresis, the nicotine addiction-associated proteins were extensively screened in the striatum of rat brains. The nicotine addiction was developed by repeated nicotine injection (0.4mg/kg s.c.), twice daily for 7 days, followed by one challenge injection after ...

journal_title：Biochemical and biophysical research communications

authors： Yeom M,Shim I,Lee HJ,Hahm DH

更新日期：2005-01-14 00:00:00

abstract：:Release of soluble growth hormone binding protein (GHBP) corresponding to the extracellular domain of the GH receptor (GHR) occurs via distinct mechanisms depending on species. In human, proteolysis of full length GHR results in liberation of GHBP into the extracellular medium. A putative protease responsive for GHR c...

journal_title：Biochemical and biophysical research communications

authors： Conte F,Salles JP,Raynal P,Fernandez L,Molinas C,Tauber M,Bieth E

更新日期：2002-01-18 00:00:00

abstract：:Opioid agonists either potentiate or suppress basal cAMP production in SK-N-SH cells. The inhibitory effect is mediated by PTX-sensitive GTP-binding proteins, while the stimulatory effect involves Ca++ entry and calmodulin activation. Both pathways can be activated simultaneously by opioid agonists. Low (nM) concentra...

journal_title：Biochemical and biophysical research communications

authors： Sarne Y,Rubovitch V,Fields A,Gafni M

更新日期：1998-05-08 00:00:00

abstract：:The effect of calcium channel blockers (CCBs) on type 2 diabetes is still unclear. The present study was undertaken to examine the efficacy of nifedipine, a dihydropyridine CCB, on obesity, glucose intolerance and vascular endothelial dysfunction in db/db mice (a mouse model of obesity and type 2 diabetes). db/db mice...

journal_title：Biochemical and biophysical research communications

authors： Yamamoto E,Nakamura T,Kataoka K,Tokutomi Y,Dong YF,Fukuda M,Nako H,Yasuda O,Ogawa H,Kim-Mitsuyama S

更新日期：2010-12-17 00:00:00

abstract：:Oligodendrocytes (OLGs) are cells from the central nervous system that synthesize, assemble, and maintain myelin, the multilamellar membrane that surrounds axons and facilitates the fast conduction of nerve impulses. We have shown that OLGs initiate their myelinogenic phenotype upon adhesion to GRASP, a heparin-bindin...

journal_title：Biochemical and biophysical research communications

authors： Szuchet S,Plachetzki DC,Eaton KS

更新日期：2001-05-18 00:00:00

abstract：:Stimulation of DNA synthesis by EGF was compared in cultured periportal and perivenous hepatocyte populations. Periportal hepatocytes responded to EGF more sensitive (IC50-values 20 vs 75 ng/ml) and with a higher maximal stimulation (420 vs 290%) than perivenous hepatocytes with respect to both [3H]thymidine incorpora...

journal_title：Biochemical and biophysical research communications

authors： Gebhardt R,Jonitza D

更新日期：1991-12-31 00:00:00

abstract：:Jmjd2c is a candidate oncogene that encodes histone lysine demethylase. In this study, we discovered that over-expression of Jmjd2c increased the expression of Mdm2 oncogene dependent on its demethylase activity, which led to the reduction of p53 tumor suppressor gene product in the cells. A chromatin immunoprecipitat...

journal_title：Biochemical and biophysical research communications

authors： Ishimura A,Terashima M,Kimura H,Akagi K,Suzuki Y,Sugano S,Suzuki T

更新日期：2009-11-13 00:00:00

abstract：:The alpha(1c) subunit of the cardiac L-type Ca(2+) channel, which contains the channel pore, voltage- and Ca(2+)-dependent gating structures, and drug binding sites, has been well studied in heterologous expression systems, but many aspects of L-type Ca(2+) channel behavior in intact cardiomyocytes remain poorly chara...

journal_title：Biochemical and biophysical research communications

authors： Ganesan AN,O'Rourke B,Maack C,Colecraft H,Sidor A,Johns DC

更新日期：2005-04-08 00:00:00

abstract：:The effect of tributyltin (TBT) on human intestinal epithelial cell functions was investigated by using human intestinal Caco-2 cell monolayers. We paid particular attention to the effect of TBT on two barrier functions: the tight junction as a physical barrier and MDR1/P-glycoprotein as a biological barrier. A loss o...

journal_title：Biochemical and biophysical research communications

authors： Tsukazaki M,Satsu H,Mori A,Sugita-Konishi Y,Shimizu M

更新日期：2004-03-19 00:00:00

abstract：:We examined the role of ras proteins in mitogenesis and proliferation of A10 smooth muscle cells. An assay was developed in which a dominant negative ras gene could be transiently expressed in vascular smooth muscle cells. As opposed to cells transfected with a control plasmid, those transfected with a dominant negati...

journal_title：Biochemical and biophysical research communications

authors： Irani K,Herzlinger S,Finkel T

更新日期：1994-08-15 00:00:00

abstract：:A fusion protein (bFGF-rMLA), containing the mitogen basic fibroblast growth factor (bFGF) and the cytotoxic component of rViscumin (recombinant mistletoe lectin), the enzymatic A-chain (rMLA), was expressed in Escherichia coli, purified, and functionally characterized. bFGF-rMLA is cytotoxic for mouse B16 melanoma ce...

journal_title：Biochemical and biophysical research communications

authors： Schmidt A,Möckel B,Eck J,Langer M,Gauert M,Zinke H

更新日期：2000-10-22 00:00:00

abstract：:The mechanism(s) involved in the generation of free radicals in human leukocytes by phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMP), lipopolysaccharide (LPS), arachidonic acid (AA), and recombinant-tumor necrosis factor-1-alpha (r-TNF-1 alpha) was investigated. Calmodulin antagonists, chlo...

journal_title：Biochemical and biophysical research communications

authors： Das UN,Padma M,Sagar PS,Ramesh G,Koratkar R

更新日期：1990-03-30 00:00:00