Stimulation of free radical generation in human leukocytes by various agents including tumor necrosis factor is a calmodulin dependent process.

Abstract:

:The mechanism(s) involved in the generation of free radicals in human leukocytes by phorbol myristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (FMP), lipopolysaccharide (LPS), arachidonic acid (AA), and recombinant-tumor necrosis factor-1-alpha (r-TNF-1 alpha) was investigated. Calmodulin antagonists, chlorpromazine and trifluoperazine, inhibited free radical generation in human leukocytes by these stimulants. Dexamethosone, an inhibitor of phospholipase A2, could also block free radical generation in human leukocytes induced by r-TNF 1 alpha. PMA, FMP, LPS and TNF can activate phospholipase A2 and induce the release of AA from the cell membrane lipid pool. AA induced free radical generation in human leukocytes can be inhibited by calmodulin antagonists. Hence, it is likely that calmodulin dependent events play a crucial role in the generation of free radicals by human leukocytes in response to various stimulants including TNF.

authors

Das UN,Padma M,Sagar PS,Ramesh G,Koratkar R

doi

10.1016/0006-291x(90)90626-x

subject

Has Abstract

pub_date

1990-03-30 00:00:00

pages

1030-6

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(90)90626-X

journal_volume

167

pub_type

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