当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Reverse engineering the L-type Ca2+ channel alpha1c subunit in adult cardiac myocytes using novel adenoviral vectors.

Reverse engineering the L-type Ca2+ channel alpha1c subunit in adult cardiac myocytes using novel adenoviral vectors.

Abstract：

:The alpha(1c) subunit of the cardiac L-type Ca(2+) channel, which contains the channel pore, voltage- and Ca(2+)-dependent gating structures, and drug binding sites, has been well studied in heterologous expression systems, but many aspects of L-type Ca(2+) channel behavior in intact cardiomyocytes remain poorly characterized. Here, we develop adenoviral constructs with E1, E3 and fiber gene deletions, to allow incorporation of full-length alpha(1c) gene cassettes into the adenovirus backbone. Wild-type (alpha(1c-wt)) and mutant (alpha(1c-D-)) Ca(2+) channel adenoviruses were constructed. The alpha(1c-D-) contained four point substitutions at amino acid residues known to be critical for dihydropyridine binding. Both alpha(1c-wt) and alpha(1c-D-) expressed robustly in A549 cells (peak L-type Ca(2+) current (I(CaL)) at 0 mV: alpha(1c-wt) -9.94+/-1.00pA/pF, n=9; alpha(1c-D-) -10.30pA/pF, n=12). I(CaL) carried by alpha(1c-D-) was markedly less sensitive to nitrendipine (IC(50) 17.1 microM) than alpha(1c-wt) (IC(50) 88 nM); a feature exploited to discriminate between engineered and native currents in transduced guinea-pig myocytes. 10 microM nitrendipine blocked only 51+/-5% (n=9) of I(CaL) in alpha(1c-D-)-expressing myocytes, in comparison to 86+/-8% (n=9) of I(CaL) in control myocytes. Moreover, in 20 microM nitrendipine, calcium transients could still be evoked in alpha(1c-D-)-transduced cells, but were largely blocked in control myocytes, indicating that the engineered channels were coupled to sarcoplasmic reticular Ca(2+) release. These alpha(1c) adenoviruses provide an unprecedented tool for structure-function studies of cardiac excitation-contraction coupling and L-type Ca(2+) channel regulation in the native myocyte background.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Ganesan AN,O'Rourke B,Maack C,Colecraft H,Sidor A,Johns DC

doi

10.1016/j.bbrc.2005.02.015

subject

Has Abstract

pub_date

2005-04-08 00:00:00

pages

749-54

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(05)00260-3

journal_volume

329

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Association of functional microsatellites in the human type I collagen alpha2 chain (COL1A2) gene with systemic sclerosis.

    abstract：:Systemic sclerosis (SSc) or scleroderma is a generalized disorder of connective tissue. The etiology is poorly understood; however, both genetic and environmental factors have been implicated. To investigate the disease-susceptible gene for SSc, we examined the association of the disease with a gene (COL1A2) for type ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2731

    authors： Hata R,Akai J,Kimura A,Ishikawa O,Kuwana M,Shinkai H

    更新日期：2000-05-27 00:00:00

  • α-Melanocyte stimulating hormone attenuates dexamethasone-induced osteoblast damages through activating melanocortin receptor 4-SphK1 signaling.

    abstract：:Long-term glucocorticoid (GC) usage may cause non-traumatic femoral head osteonecrosis. Dexamethasone (Dex) is shown to exert potent cytotoxic effect to osteoblasts. Here, we investigated the potential activity of α-melanocyte stimulating hormone (α-MSH) against the process. Our data revealed that pretreatment of α-MS...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.11.104

    authors： Guo S,Xie Y,Fan JB,Ji F,Wang S,Fei H

    更新日期：2016-01-08 00:00:00

  • Epigenetic involvement of Alien/ESET complex in thyroid hormone-mediated repression of E2F1 gene expression and cell proliferation.

    abstract：:The ligand-bound thyroid hormone receptor (TR) is known to repress via a negative TRE (nTRE) the expression of E2F1, a key transcription factor that controls the G1/S phase transition. Alien has been identified as a novel interacting factor of E2F1 and acts as a corepressor of E2F1. The detailed molecular mechanism by...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.10.130

    authors： Hong W,Li J,Wang B,Chen L,Niu W,Yao Z,Baniahmad A

    更新日期：2011-12-02 00:00:00

  • Sequence homology between prokaryotic and eukaryotic forms of serine hydroxymethyltransferase.

    abstract：:The sequence of tryptic and chymotryptic peptides from cytosolic and mitochondrial rabbit liver serine hydroxymethyltransferase are compared to the proposed sequence of a protein coded for by the glyA gene of Escherichia coli. The E. coli glyA gene is believed to code for serine hydroxymethyltransferase. Extensive seq...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80242-3

    authors： Barra D,Martini F,Angelaccio S,Bossa F,Gavilanes F,Peterson D,Bullis B,Schirch L

    更新日期：1983-11-15 00:00:00

  • Lipid composition of membrane rafts, isolated with and without detergent, from the spleen of a mouse model of Gaucher disease.

    abstract：:Biological membranes are composed of functionally relevant liquid-ordered and liquid-disordered domains that coexist. Within the liquid-ordered domains are low-density microdomains known as rafts with a unique lipid composition that is crucial for their structure and function. Lipid raft composition is altered in sphi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.009

    authors： Hattersley KJ,Hein LK,Fuller M

    更新日期：2013-12-06 00:00:00

  • In vitro and in vivo antagonists against parathyroid hormone-related protein.

    abstract：:Four analogues of parathyroid hormone-related protein (PTHrP), PTHrP(7-34)NH2, (10-34)NH2, (15-34)NH2 and (20-34)NH2, were synthesized and their antagonistic activity against PTHrP(1-34) was examined in vitro and in vivo. In vitro studies revealed that all four analogues antagonized PTHrP-stimulated cyclic AMP product...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92826-x

    authors： Nagasaki K,Yamaguchi K,Miyake Y,Hayashi C,Honda S,Urakami K,Miki K,Kimura S,Watanabe T,Abe K

    更新日期：1989-02-15 00:00:00

  • Automated quantification of FISH signals in urinary cells enables the assessment of chromosomal aberration patterns characteristic for bladder cancer.

    abstract：:Targeting the centromeres of chromosomes 3, 7, 17 (CEP3, 7, 17) and the 9p21-locus (LSI9p21) for diagnosing bladder cancer (BC) is time- and cost-intensive and requires a manual investigation of the sample by a well-trained investigator thus overall limiting its use in clinical diagnostics and large-scaled epidemiolog...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.04.137

    authors： Köhler CU,Martin L,Bonberg N,Behrens T,Deix T,Braun K,Noldus J,Jöckel KH,Erbel R,Sommerer F,Tannapfel A,Harth V,Käfferlein HU,Brüning T

    更新日期：2014-06-13 00:00:00

  • Loading alters actin dynamics and up-regulates cofilin gene expression in chondrocytes.

    abstract：:Chondrocyte mechanotransduction in response to mechanical loading is essential for the health and homeostasis of articular cartilage. The actin cytoskeleton has been implicated in cell mechanics and mechanotransduction. This study tests the hypothesis that loading modulates actin dynamics and organisation with subsequ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.06.185

    authors： Campbell JJ,Blain EJ,Chowdhury TT,Knight MM

    更新日期：2007-09-21 00:00:00

  • Induction of apoptosis and inhibition of cell growth by developmental regulator hTBX5.

    abstract：:T box (Tbx) genes are a large family of transcription regulators that play critical roles in invertebrate and vertebrate development. Mutations in Tbx5 gene have been found to cause Holt-Oram syndrome (HOS) in humans. Partial dysfunction of TBX5 in mouse also causes HOS phenotype. Little is known about its molecular a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(02)02142-3

    authors： He ML,Chen Y,Peng Y,Jin D,Du D,Wu J,Lu P,Lin MC,Kung HF

    更新日期：2002-09-20 00:00:00

  • An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression.

    abstract：:In higher plants, a cis-acting element, DRE/CRT, is involved in gene expression responsive to drought and low-temperature stress. To understand signal transduction pathways from the cold stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB1A and CBF1 in Arabidopsis thalian...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9267

    authors： Shinwari ZK,Nakashima K,Miura S,Kasuga M,Seki M,Yamaguchi-Shinozaki K,Shinozaki K

    更新日期：1998-09-08 00:00:00

  • Effect of Pseudomonas aeruginosa exotoxin-A on the synthesis and secretion of proteins in isolated rat pancreatic acini.

    abstract：:Exposure of isolated rat dispersed pancreatic acini to increasing concentrations (10 to 1000 ng/ml) of purified exotoxin-A from Pseudomonas aeruginosa resulted in a progressive inhibition of 3H-leucine incorporation into "cellular" (those remaining in the cells) and "secretory" (those released into the medium) protein...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(85)80144-3

    authors： Geokas MC,Vesenka GD,Dubick MA,Majumdar AP

    更新日期：1985-02-28 00:00:00

  • Identification and heterologous expression of an isoquinolinequinone biosynthetic gene cluster from Streptomyces albus J1074.

    abstract：:Nitrogen heterocycle small molecules display various pharmaceutically important bioactivities and have great potential in drug development and application. Microbes are an important source for discovering nitrogen heterocycle natural products, and the elucidation of their biosynthetic pathways in microbes facilitates ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.12.093

    authors： Chai S,Cao M,Feng Z

    更新日期：2021-01-11 00:00:00

  • Specific binding sites for [3H]inositol(1,3,4,5)tetrakisphosphate on membranes of HL-60 cells.

    abstract：:Membranes of HL-60 cells were shown to possess saturable binding sites for [3H]inositol(1,3,4,5)tetrakisphosphate, with nanomolar affinity (KD = 90 nM) and a density of 250 fmol/mg protein. The specificity of the binding sites for Ins(1,3,4,5)P4 was assessed by competition studies utilising a variety of inositol polyp...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90421-9

    authors： Bradford PG,Irvine RF

    更新日期：1987-12-16 00:00:00

  • Distinct role of the intracellular C-terminus for subcellular expression, shedding and function of the murine transmembrane chemokine CX3CL1.

    abstract：:The transmembrane chemokine CX3CL1 is expressed on the endothelial surface and promotes leukocyte adhesion and transmigration by receptor interaction via its extracellular chemokine domain. Since little is known about its intracellular C-terminus, we examined the consequences of C-terminal truncation on cellular distr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.03.139

    authors： Andrzejewski MG,Koelsch A,Kogel T,Dreymueller D,Schwarz N,Ludwig A

    更新日期：2010-04-30 00:00:00

  • A monoclonal antibody against acetylcholinesterase inhibits the formation of amyloid fibrils induced by the enzyme.

    abstract：:A monoclonal antibody (mAb) 25B1 directed against fetal bovine-serum acetylcholinesterase (FBS AChE) was used to examine the ability of the cholinergic enzyme to promote the assembly of amyloid-beta peptides (A beta) into Alzheimerś fibrils. This mAb binds to the peripheral anionic site of the enzyme and allostericall...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6357

    authors： Reyes AE,Perez DR,Alvarez A,Garrido J,Gentry MK,Doctor BP,Inestrosa NC

    更新日期：1997-03-27 00:00:00

  • Metastable primordial germ cell-like state induced from mouse embryonic stem cells by Akt activation.

    abstract：:Specification to primordial germ cells (PGCs) is mediated by mesoderm-induction signals during gastrulation. We found that Akt activation during in vitro mesodermal differentiation of embryonic stem cells (ESCs) generated self-renewing spheres with differentiation states between those of ESCs and PGCs. Essential regul...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.005

    authors： Yamano N,Kimura T,Watanabe-Kushima S,Shinohara T,Nakano T

    更新日期：2010-02-12 00:00:00

  • Involvement of Rabring7 in EGF receptor degradation as an E3 ligase.

    abstract：:Rab7, a member of the Rab family small G proteins, is involved in the late stage of the endocytic pathway. We previously identified a Rab7 target protein, Rabring7, which contains a RING finger domain at its C termini, but the precise role of Rabring7 remains unknown. In this study, we demonstrate using an in vitro ub...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.04.052

    authors： Sakane A,Hatakeyama S,Sasaki T

    更新日期：2007-06-15 00:00:00

  • Methionine controls insulin/mammalian target of rapamycin complex 1 activity by modulating tuberous sclerosis complex 2 stability.

    abstract：:Tuberous sclerosis complex 2 (TSC2) is a tumor-suppressor protein that is partially regulated by insulin, energy, oxygen, and growth factors. Mutations in the TSC2 gene and loss of TSC2 promote cell growth by the mammalian target of rapamycin complex 1 (mTORC1) activation. Furthermore, S-adenosylmethionine (SAM) senso...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2021.01.033

    authors： Gen S,Matsumoto Y,Suzuki T,Inoue J,Yamamoto Y

    更新日期：2021-01-19 00:00:00

  • Protein-tyrosine phosphatase inhibition by a peptide containing the phosphotyrosyl mimetic, L-O-malonyltyrosine.

    abstract：:Peptides containing phosphonate based non-hydrolyzable phosphotyrosyl (pTyr) mimetics previously have been shown to be competitive inhibitors of protein-tyrosine phosphatases (PTPs). These agents suffer from low cellular penetration which is partially attributable to ionization of the phosphonate group at physiologica...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1573

    authors： Kole HK,Akamatsu M,Ye B,Yan X,Barford D,Roller PP,Burke TR Jr

    更新日期：1995-04-26 00:00:00

  • Function of P-glycoprotein expressed in placenta and mole.

    abstract：:We examined the expression of P-glycoprotein in human placentas and hydatidiform moles. Trophoblasts in all the examined placentas and moles expressed P-glycoprotein, and the size of the P-glycoprotein was smaller than that in multidrug-resistant human epidermoid carcinoma KB cells. The P-glycoprotein in the placenta ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6855

    authors： Nakamura Y,Ikeda S,Furukawa T,Sumizawa T,Tani A,Akiyama S,Nagata Y

    更新日期：1997-06-27 00:00:00

  • Ca(2+) homeostasis and endoplasmic reticulum (ER) stress: An integrated view of calcium signaling.

    abstract：:Cellular Ca(2+) homeostasis is maintained through the integrated and coordinated function of Ca(2+) transport molecules, Ca(2+) buffers and sensors. These molecules are associated with the plasma membrane and different cellular compartments, such as the cytoplasm, nucleus, mitochondria, and cellular reticular network,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2015.02.004

    authors： Krebs J,Agellon LB,Michalak M

    更新日期：2015-04-24 00:00:00

  • Cloning of a major heparan sulfate proteoglycan from brain and identification as the rat form of glypican.

    abstract：:We have obtained the complete coding sequence of a highly conserved heparan sulfate proteoglycan which we previously characterized biochemically after isolation from rat brain. An open reading frame of 558 amino acids encodes a protein with a molecular mass of 62 kDa containing three peptide sequences present in the i...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92398-h

    authors： Karthikeyan L,Maurel P,Rauch U,Margolis RK,Margolis RU

    更新日期：1992-10-15 00:00:00

  • Identification of the transcriptional repression domain of nuclear factor 1-A.

    abstract：:We previously showed that nuclear factor 1-A (NF1-A) binds to the silencer elements in the glutathione transferase P (GST-P) gene, and the carboxy terminal region of NF1-A represses the transcription activity of human metallothionein IIA (hMTIIA) promoter. In this study, we identified a repression region which is divi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7382

    authors： Osada S,Ikeda T,Xu M,Nishihara T,Imagawa M

    更新日期：1997-09-29 00:00:00

  • Cloning and characterization of a novel human gene RNF38 encoding a conserved putative protein with a RING finger domain.

    abstract：:RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a large family of functionally distinct proteins. We describe the cloning and characterization of a novel human transcript RNF38 encoding a new member of the RING finger protein family. The complete mRNA consists of about 6.8 kb widely expr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/S0006-291X(02)00584-3

    authors： Eisenberg I,Hochner H,Levi T,Yelin R,Kahan T,Mitrani-Rosenbaum S

    更新日期：2002-06-28 00:00:00

  • Impact of methionine oxidation on calmodulin structural dynamics.

    abstract：:We have used electron paramagnetic resonance (EPR) to examine the structural impact of oxidizing specific methionine (M) side chains in calmodulin (CaM). It has been shown that oxidation of either M109 or M124 in CaM diminishes CaM regulation of the muscle calcium release channel, the ryanodine receptor (RyR), and tha...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.11.091

    authors： McCarthy MR,Thompson AR,Nitu F,Moen RJ,Olenek MJ,Klein JC,Thomas DD

    更新日期：2015-01-09 00:00:00

  • The involvement of an LPS inducible I kappa B kinase in endotoxin tolerance.

    abstract：:When human ovarian carcinoma cells are challenged with endotoxin, an I kappa B kinase is transiently induced within 3 to 5 min. This enzyme activity causes the hyperphosphorylation and subsequent degradation of I kappa B which allows NF-kappa B to translocate to the nucleus where it activates transcription. When endot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6337

    authors： Kohler NG,Joly A

    更新日期：1997-03-27 00:00:00

  • Long-lasting vasoconstriction and efficient regional extraction of endothelin-1 in human splanchnic and renal tissues.

    abstract：:Six healthy subjects were given endothelin-1, intravenously in a dose of 4 pmol.kg-1.min-1 for 20 min. Blood samples were drawn from arterial, hepatic and renal vein catheters for determinations of splanchnic and renal blood flows and the extraction of endothelin-1 in these vascular beds. Intravenous infusion of endot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81336-1

    authors： Weitzberg E,Ahlborg G,Lundberg JM

    更新日期：1991-11-14 00:00:00

  • Activation of simian virus 40 promoter by HTLV-I Tax protein: role of NF-kappaB and CBP.

    abstract：:HTLV-I is implicated with adult T-cell leukemia and certain other clinical disorders. The viral Tax protein is regarded as a key element in HTLV-I pathogenicity due to its ability to activate a wide variety of cellular regulatory factors. As such, Tax may likely activate also latent infection of certain other pathogen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.04.137

    authors： Tabakin-Fix Y,Huleihel M,Aboud M

    更新日期：2004-06-11 00:00:00

  • Ang II induces capillary formation from endothelial cells via the AT1R-dependent inositol requiring enzyme 1 pathway.

    abstract：:Previous studies have demonstrated an important interaction between angiotension II type 1 receptor (AT1R) and angiotension II (Ang II) -induced capillary formation from endothelial cells and vascular endothelial growth factor (VEGF). However, the underlying mechanism remains elusive. Recent studies revealed that the ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.03.113

    authors： Wang X,Bai YP,Hong D,Gao HC,Li LF,Li CC,Zhu LP,Sun Q,Zhang GG

    更新日期：2013-05-10 00:00:00

  • CAAT/enhancer binding proteins directly modulate transcription from the peroxisome proliferator-activated receptor gamma 2 promoter.

    abstract：:The CCAAT/enhancer binding proteins (C/EBPs) and the peroxisome proliferator-activated receptors (PPARs) together regulate adipogenesis. The current work uses co-transfection studies to examine the C/ EBP dependence of PPAR gamma 2 transcription. Both C/ EBP alpha and C/EBP delta expression vectors activated transcrip...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7627

    authors： Clarke SL,Robinson CE,Gimble JM

    更新日期：1997-11-07 00:00:00