Effect of Pseudomonas aeruginosa exotoxin-A on the synthesis and secretion of proteins in isolated rat pancreatic acini.

abstract：:MazF is a sequence-specific endoribonuclease or mRNA interferase, which cleaves RNA at a specific sequence. Since the expression of a specific gene or a group of specific genes can be regulated by MazF, expanding the repertoire of recognition sequences by MazF mRNA interferases is highly desirable for biotechnological...

journal_title：Biochemical and biophysical research communications

authors： Ishida Y,Inouye K,Ming O,Inouye M

更新日期：2019-10-20 00:00:00

abstract：:Chloroquine inhibited carbachol-induced amylase release in a dose-dependent fashion in rat pancreatic acini; cholecystokinin- and bombesin-induced secretory responses were almost unchanged by the antimalarial drug. The inhibition of carbachol-induced amylase release by chloroquine was competitive in nature with a Ki o...

journal_title：Biochemical and biophysical research communications

authors： Habara Y,Williams JA,Hootman SR

更新日期：1986-06-13 00:00:00

abstract：:We have identified a human pituitary protein as a novel glycosylated variant of hGH. Isolation of the denatured protein included separation of human pituitary extract by Sephadex G-100 chromatography in ammonium bicarbonate, followed by Sephadex G-100 chromatography in 10% acetic acid, with subsequent DEAE Sephacryl c...

journal_title：Biochemical and biophysical research communications

authors： Haro LS,Lewis UJ,Garcia M,Bustamante J,Martinez AO,Ling NC

更新日期：1996-11-12 00:00:00

abstract：:Integral membrane lysophospholipid acyltransferases (AT) are involved in many reactions that produce phospholipids and triglycerides. Enzymes that utilize lysophosphatidic acid (LPA) as an acceptor substrate have been termed LPAATs, and several are members of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) ge...

journal_title：Biochemical and biophysical research communications

authors： Schmidt JA,Yvone GM,Brown WJ

更新日期：2010-07-09 00:00:00

abstract：:GroEL-like particles of Thermus aquaticus are homo-oligomeric complexes of two stacked seven member rings, sedimenting in the gradient at 20S. The apparent molecular mass of the native particles is 820,000 (+/- 30,000). The protein complex is composed with one polypeptide of M(r) 59,000. Immunoblotting results and N-t...

journal_title：Biochemical and biophysical research communications

authors： Mikulík K,Benada O

更新日期：1993-12-15 00:00:00

abstract：:Virosomes as membranous vesicles with viral fusion protein in their membrane are versatile vehicles for cargo delivery. The vesicular stomatitis virus glycoprotein (VSV-G) is a common fusogenic protein used in virosome preparation. This glycoprotein has been used in liposomal systems so far, but in this study, we have...

journal_title：Biochemical and biophysical research communications

authors： Asadikaram G,Poustforoosh A,Pardakhty A,Torkzadeh-Mahani M,Nematollahi MH

更新日期：2021-01-01 00:00:00

abstract：:Phosphofructokinase-2 from rat liver is inhibited by phosphoenolpyruvate and ADP. Phosphoenolpyruvate reduces the maximum activity in respect to fructose-6-phosphate and ATP but does not give rise to complete inhibition of phosphofructokinase-2. ADP increases the apparent Michaelis constant of the enzyme for ATP and l...

journal_title：Biochemical and biophysical research communications

authors： Kretschmer M,Hofmann E

更新日期：1984-11-14 00:00:00

abstract：:Trichosanthin (TCS) is a type I ribosome-inactivating protein that inhibits the replication of both human immunodeficiency virus type 1 (HIV-1) and herpes simplex virus type 1 (HSV-1). The mechanism of inhibition is not clear. This investigation explored the effects of TCS on the stages of HSV-1 infection in Hep-2 cel...

journal_title：Biochemical and biophysical research communications

authors： He DX,Tam SC

更新日期：2010-11-26 00:00:00

abstract：:The Forkhead/Fox transcription factor Foxc2 is a critical regulator of vascular development. However, the role of Foxc2 in pathological angiogenesis in cancer remains unknown. Here we show that FoxC2 is highly expressed in human breast and colonic tumors and in the tumor endothelium in human and mouse melanomas. Using...

journal_title：Biochemical and biophysical research communications

authors： Sano H,Leboeuf JP,Novitskiy SV,Seo S,Zaja-Milatovic S,Dikov MM,Kume T

更新日期：2010-02-05 00:00:00

abstract：:The ferromagnetic transduction model proposed by J.L. Kirschvink (Phys. Rev. A (1992) 46, 2178-2184) suggests that the coupling of biogenic magnetic particles in the human brain to mechanosensitive membrane ion gates may provide a mechanism for interactions of environmental magnetic fields with humans. Extremely low f...

journal_title：Biochemical and biophysical research communications

authors： Dobson J,St Pierre T

更新日期：1996-10-23 00:00:00

abstract：:We isolated genomic DNA containing the entire sequence of ZF5, which was originally identified by its ability to repress the mouse c-myc promoter and which was characterized as one of the POZ (Poxvirus and zinc finger) proteins. The POZ motif is a protein-protein interaction interface found at the N-terminal region of...

journal_title：Biochemical and biophysical research communications

authors： Yokoro K,Yanagidani A,Obata T,Yamamoto S,Numoto M

更新日期：1998-05-29 00:00:00

abstract：:Platelet activating factor was isolated from the lesional scale of psoriatic patients using the method described by Bligh and Dyer (8). The extract was subjected to thin layer chromatography, and the region of the plate co-migrating with platelet activating factor removed. A portion of each sample was assayed for aggr...

journal_title：Biochemical and biophysical research communications

authors： Mallet AI,Cunningham FM

更新日期：1985-01-16 00:00:00

abstract：:Butyrate, a short-chain fatty acid produced in the colon, as well as its prodrug tributyrin, reduce proliferation and increase differentiation of colon cancer cells. p21(Waf1/Cip1) and p27(Kip1) are negative regulators of cell cycle and are thought to have a key function in the differentiation of various cell lines. W...

journal_title：Biochemical and biophysical research communications

authors： Gaschott T,Wächtershäuser A,Steinhilber D,Stein J

更新日期：2001-04-27 00:00:00

abstract：:A cDNA encoding non-selective type (ETB) of endothelin receptor was isolated from a human liver cDNA library. The cDNA had an open reading frame encoding a protein of 442 amino acid residues with a relative Mr of 49,643. The deduced amino acid sequence of human ETB receptor was 88% and 64% identical to those of rat lu...

journal_title：Biochemical and biophysical research communications

authors： Nakamuta M,Takayanagi R,Sakai Y,Sakamoto S,Hagiwara H,Mizuno T,Saito Y,Hirose S,Yamamoto M,Nawata H

更新日期：1991-05-31 00:00:00

abstract：:A cDNA for the rat bone morphogenetic protein (BMP) type IA receptor (BMPR-IA) was isolated from a dental pulp cell cDNA library. The rat BMPR-IA cDNA encodes a protein of 532 amino acids with a single transmembrane domain and a putative serine/threonine kinase domain. The overall amino acid sequence identity between ...

journal_title：Biochemical and biophysical research communications

authors： Takeda K,Oida S,Ichijo H,Iimura T,Maruoka Y,Amagasa T,Sasaki S

更新日期：1994-10-14 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, Ca(2+) signaling mediated by the Ca(2+)/calmodulin dependent phosphatase, calcineurin, is required for survival during environmental stress. One role of the phosphatase under these conditions is to activate gene expression through its regulation of the Crz1p ("crazy") transcripti...

journal_title：Biochemical and biophysical research communications

authors： Cyert MS

更新日期：2003-11-28 00:00:00

abstract：:The cDNA of a cystein peptidase inhibitor was isolated from sugarcane and expressed in Escherichia coli. The protein, named canecystatin, has previously been shown to exert antifungal activity on the filamentous fungus Trichoderma reesei. Herein, the inhibitory specificity of canecystatin was further characterized. It...

journal_title：Biochemical and biophysical research communications

authors： Oliva ML,Carmona AK,Andrade SS,Cotrin SS,Soares-Costa A,Henrique-Silva F

更新日期：2004-08-06 00:00:00

abstract：:Smad2 is a crucial component of intracellular signaling by transforming growth factor-β (TGFβ). Here we describe that Smad2 is glycosylated, which is a novel for Smad2 post-translational modification. We showed that the Smad2 glycosylation was inhibited upon treatment of cells with 17β-estradiol, and was enhanced in c...

journal_title：Biochemical and biophysical research communications

authors： Gotoh T,Iwahana H,Kannan S,Marei RG,Mousa H,Elgamal M,Souchelnytskyi S

更新日期：2020-01-22 00:00:00

abstract：:We have characterized the genomic structure of the mouse Zfp148 gene encoding Beta-Enolase Repressor Factor-1 (BERF-1), a Kruppel-like zinc finger protein involved in the transcriptional regulation of several genes, which is also termed ZBP-89, BFCOL1. The cloned Zfp148 gene spans 110 kb of genomic DNA encompassing th...

journal_title：Biochemical and biophysical research communications

authors： Feo S,Antona V,Cammarata G,Cavaleri F,Passantino R,Rubino P,Giallongo A

更新日期：2001-04-27 00:00:00

abstract：:Adherens junction-associated protein-1 (AJAP1), also called SHREW1, was first discovered as a novel component of adherens junctions in 2004. In later studies, AJAP1 was found to suppress invasion and predict recurrence of some tumors. Apart from its function as a putative tumor suppressor, AJAP1 is still poorly unders...

journal_title：Biochemical and biophysical research communications

authors： Zhang M,Zhou X,Jiang W,Li M,Zhou R,Zhou S

更新日期：2020-04-16 00:00:00

abstract：:Fructose is a key dietary factor in the development of nonalcoholic fatty liver disease (NAFLD). Here we investigated whether WAY-362450 (WAY), a potent synthetic and orally active FXR agonist, protects against fructose-induced steatosis and the underlying mechanisms. C57BL/6J mice, fed 30% fructose for 8 weeks, were ...

journal_title：Biochemical and biophysical research communications

authors： Liu X,Xue R,Ji L,Zhang X,Wu J,Gu J,Zhou M,Chen S

更新日期：2014-07-18 00:00:00

abstract：:The association of endogenous synenkephalin and met-enkephalin containing peptides with the membrane of bovine chromaffin granules and physicochemical characteristics of this association were studied. The associated materials were only released at a non physiological pH range and this effect was enhanced with growing ...

journal_title：Biochemical and biophysical research communications

authors： Ase A,Vindrola O,Aloyz R,Inés M,Vida R,Finkielman S,Nahmod V

更新日期：1989-02-15 00:00:00

abstract：:The green tea gallocatechins, (-)-epigallocatechin-3-O-gallate (EGCG) (IC(50) = 0.69 microM), (-)-gallocatechin-3-O-gallate (GCG) (IC(50) = 0.67 microM), (-)-epicatechin-3-O-gallate (ECG) (IC(50) = 1.3 microM), and theasinensin A (IC(50) = 0.13 microM), were found to be potent and selective inhibitors of rat squalene ...

journal_title：Biochemical and biophysical research communications

authors： Abe I,Seki T,Umehara K,Miyase T,Noguchi H,Sakakibara J,Ono T

更新日期：2000-02-24 00:00:00

abstract：:The effects of endothelins (ETs) on the production of stromelysins, a sub-family of matrix metalloproteinases, were examined in cultured astrocytes. The treatment of cultured rat astrocytes with ET-1 increased stromelysin-1 mRNA levels, while stromelysin-2 and -3 mRNAs were not affected. Immunocytochemical observation...

journal_title：Biochemical and biophysical research communications

authors： Koyama Y,Tanaka K

更新日期：2008-07-11 00:00:00

abstract：:Immediate-early genes (IEGs) are useful for mapping active brain regions in various vertebrates. Here we identified a c-fos homologue gene in medaka and demonstrated that the amounts of c-fos transcripts and proteins in the medaka brain increased in relation to an artificially evoked seizure, suggesting that the homol...

journal_title：Biochemical and biophysical research communications

authors： Okuyama T,Suehiro Y,Imada H,Shimada A,Naruse K,Takeda H,Kubo T,Takeuchi H

更新日期：2011-01-07 00:00:00

abstract：:Tendon calcification has been widely regarded by researchers to result from the osteogenic differentiation of Tendon-Derived Stem Cells (TDSCs) and ectopic mineralization caused by the calcification of cellular matrix. Recent studies have revealed a correlation between the Mg(2+)/Ca(2+) balance and the degeneration or...

journal_title：Biochemical and biophysical research communications

authors： Yue J,Jin S,Li Y,Zhang L,Jiang W,Yang C,Du J

更新日期：2016-09-09 00:00:00

abstract：:Transforming growth factor-beta (TGF-beta) has been implicated in the development of diabetic glomerulopathy. In order to evaluate a role of Smad3, one of the major signaling molecules downstream of TGF-beta, in the pathogenesis of diabetic glomerulopathy, Smad3-null mice were made diabetic with streptozotocin injecti...

journal_title：Biochemical and biophysical research communications

authors： Fujimoto M,Maezawa Y,Yokote K,Joh K,Kobayashi K,Kawamura H,Nishimura M,Roberts AB,Saito Y,Mori S

更新日期：2003-06-13 00:00:00

abstract：:Human prothrombin kringle-2 and its partial peptide, NSA9 (NSAVQLVEN), have been reported to have potent anti-angiogenic activities. Here, the internalization mechanism of NSA9 into bovine capillary endothelial (BCE) cells was examined using lactate dehydrogenase (LDH) release assay, fluorescence microscopy, and flow ...

journal_title：Biochemical and biophysical research communications

authors： Hwang HS,Kim SS

更新日期：2005-09-23 00:00:00

abstract：:Mesenchymal Stem Cells are known to engraft and integrate into the architecture of colorectal tumours, with little known regarding their fate following engraftment. This study aimed to investigate mediators of Mesenchymal Stem Cell (MSC) and colon cancer cell (CCC) interactions. Mesenchymal Stem Cells and colon cancer...

journal_title：Biochemical and biophysical research communications

authors： Hogan NM,Joyce MR,Murphy JM,Barry FP,O'Brien T,Kerin MJ,Dwyer RM

更新日期：2013-06-14 00:00:00

abstract：:The 5mC DNA methyltransferase M.HhaI can be split into two individually inactive N- and C-terminal fragments that together can form an active enzyme in vivo capable of efficiently methylating DNA. This active fragment pair was identified by creating libraries of M.HhaI gene fragment pairs and then selecting for the pa...

journal_title：Biochemical and biophysical research communications

authors： Choe W,Chandrasegaran S,Ostermeier M

更新日期：2005-09-09 00:00:00