Inhibitory selectivity of canecystatin: a recombinant cysteine peptidase inhibitor from sugarcane.

abstract：:The precursor of amphibian tachykinin has not been found although more than 30 tachykinins have been isolated from amphibians since 1964. In this report, two tachykinin-like peptides are identified from the skin secretions of the frog, Odorrana grahami. Their amino acid sequences are DDTEDLANKFIGLM-NH(2) (named tachyk...

journal_title：Biochemical and biophysical research communications

authors： Li J,Liu T,Xu X,Wang X,Wu M,Yang H,Lai R

更新日期：2006-12-01 00:00:00

abstract：:We have previously described a novel putative 47kDa substrate for the protein kinase Akt (designated AS47) in 3T3-L1 adipocytes. In the present study, we have found by co-immunoprecipitation that AS47 was associated with cyclic nucleotide phosphodiesterase 3B (PDE3B) in lysates of 3T3-L1 adipocytes. The patterns of ex...

journal_title：Biochemical and biophysical research communications

authors： Chavez JA,Gridley S,Sano H,Lane WS,Lienhard GE

更新日期：2006-04-21 00:00:00

abstract：:Nikkomycins act as a competitive inhibitor of chitin synthetase and display potent activities against phytopathogenic and human pathogenic fungi. sanT is located in the gene cluster of nikkomycin biosynthesis in Streptomyces ansochromogenes. Sequence analysis revealed that the deduced product of sanT has an unusual do...

journal_title：Biochemical and biophysical research communications

authors： Jia L,Tian Y,Tan H

更新日期：2007-11-03 00:00:00

abstract：:Alisol B-23-acetate (AB23A), a tetracyclic triterpenoid isolated from the rhizome of Alisma orientale, has been reported to exert anti-proliferative activities in human colon, ovarian and gastric cancer cells. However, the anti-cancer effect of this compound on human lung cancer cells has not yet been thoroughly eluci...

journal_title：Biochemical and biophysical research communications

authors： Wang J,Li H,Wang X,Shen T,Wang S,Ren D

更新日期：2018-11-10 00:00:00

abstract：:Exocytosis is regulated by exocytotic proteins, which are present in insulin-secreting beta-cells and play regulatory roles in insulin secretion. Non-insulin dependent diabetes mellitus (type 2 diabetes) is a disease characterized by impaired insulin secretion and insulin resistance. Exocytotic protein immunoreactivit...

journal_title：Biochemical and biophysical research communications

authors： Zhang W,Khan A,Ostenson CG,Berggren PO,Efendic S,Meister B

更新日期：2002-03-08 00:00:00

abstract：:To characterize mesenchymal stem cells (MSC), we compared gene expression profiles in human bone marrow MSC (11 lines) and human fibroblasts (4 lines) by RT-PCR and real time PCR. Messenger RNA levels of MHC-DR-alpha, MHC-DR-beta, MHC-DR-associated protein CD74, tissue factor pathway inhibitor-2, and neuroserpin were ...

journal_title：Biochemical and biophysical research communications

authors： Ishii M,Koike C,Igarashi A,Yamanaka K,Pan H,Higashi Y,Kawaguchi H,Sugiyama M,Kamata N,Iwata T,Matsubara T,Nakamura K,Kurihara H,Tsuji K,Kato Y

更新日期：2005-06-24 00:00:00

abstract：:Previous studies demonstrated that macrophages are difficult to transfect. In the present study, we investigated whether J774A.1 macrophages can be efficiently transfected using nucleofector technology. Nucleofection of J774A.1 macrophages with mRNA resulted in transfection efficiencies up to 75% without cell death as...

journal_title：Biochemical and biophysical research communications

authors： Van De Parre TJ,Martinet W,Schrijvers DM,Herman AG,De Meyer GR

更新日期：2005-02-04 00:00:00

abstract：:Highly purified bovine heart protein phosphatase 2A catalytic subunit lost virtually all of its activity during storage at -70 degrees. When the enzyme was preincubated with Co2+, over 35% of the original activity was restored. Freshly prepared protein phosphatase 2A purified from bovine heart was stimulated at least ...

journal_title：Biochemical and biophysical research communications

authors： Cai L,Chu Y,Wilson SE,Schlender KK

更新日期：1995-03-08 00:00:00

abstract：:Functional biopolymer scaffolds are in high demand for tissue regeneration. In this study, we incorporated functionalized CNT in collagen or silk protein solution to generate biocomposite fibers by electrospinning. The addition of CNT reinforced the strength of the scaffolds and rendered the fibers electrical conducti...

journal_title：Biochemical and biophysical research communications

authors： Chi N,Wang R

更新日期：2018-09-26 00:00:00

abstract：:Tamoxifen (TAM), an anti-estrogen compound, is widely used for chemotherapy of breast cancer, although the molecular mechanisms underlying TAM cytotoxicity are obscure. Here, we show that TAM dramatically caused degradation of vimentin (VIM) in human skin fibroblasts, in a time and dose dependent manner. Addition of c...

journal_title：Biochemical and biophysical research communications

authors： Hashimoto M,Inoue S,Ogawa S,Conrad C,Muramatsu M,Shackelford D,Masliah E

更新日期：1998-06-18 00:00:00

abstract：:Production of endothelin-1 in the cultured neonatal rat cardiac myocytes was investigated. Studies on the cultured cardiac myocytes with reverse transcription-polymerase chain reaction followed by Southern hybridization indicated that mRNA of ET-1(preproET-1 mRNA) was expressed by cardiac myocytes. ET-1 polypeptide wa...

journal_title：Biochemical and biophysical research communications

authors： Suzuki T,Kumazaki T,Mitsui Y

更新日期：1993-03-31 00:00:00

abstract：:Transcription factors belonging to the CCAAT-enhancer binding protein (C/EBP) family have been implicated in the regulation of gene expression during growth, differentiation, apoptosis, and inflammation. Autoregulation is relatively common in the modulation of C/EBP gene expression and, for the human and murine C/EBPa...

journal_title：Biochemical and biophysical research communications

authors： Davies GE,Sabatakos G,Cryer A,Ramji DP

更新日期：2000-05-10 00:00:00

abstract：:FOXK2, which belongs to the fork head DNA binding protein family, has been shown to play a critical role in tumorigenesis. Here, we detected FOXK2 expression and its clinical significance in colorectal cancer, which has not been fully investigated before. Results from public database and our cohort indicated that FOXK...

journal_title：Biochemical and biophysical research communications

authors： Qian Y,Xia S,Feng Z

更新日期：2017-01-29 00:00:00

abstract：:The phosphatidylinositol-3-kinase (PI3K)/AKT axis and the Nuclear Factor kappa B (NFκB) pathway play critical roles in macrophage survival. In cells other than macrophages proper operation of those two pathways requires Ca²(+) influx into the cell, but if that is the case in macrophages remains unexplored. In the pres...

journal_title：Biochemical and biophysical research communications

authors： Tano JY,Vazquez G

更新日期：2011-04-08 00:00:00

abstract：:Our objective was to determine whether hydroxylamine is a possible intermediate in the oxidative conversion of L-arginine to nitric oxide. Vasorelaxation by hydroxylamine is known to be mediated by nitric oxide. The vasorelaxant properties of hydroxylamine were examined using rat aortic rings and an isolated rat lung ...

journal_title：Biochemical and biophysical research communications

authors： DeMaster EG,Raij L,Archer SL,Weir EK

更新日期：1989-08-30 00:00:00

abstract：:Using a subtraction cloning approach we had previously isolated a series of murine cDNA clones representing the genes predominantly expressed in the embryonic brain and down-regulated during development. We now report that one of these cDNA clones encodes a novel type of GTP-binding protein. The predicted protein of 4...

journal_title：Biochemical and biophysical research communications

authors： Sazuka T,Tomooka Y,Ikawa Y,Noda M,Kumar S

更新日期：1992-11-30 00:00:00

abstract：:Sustained antibiotic release is important to prevent the infections after surgical treatments such as guided tissue regeneration (GTR). Electrospinning provides a simple way to fabricate nanofibers for drug delivery. In this study, a simple method to achieve sustained antibiotic release by introducing mesoporous silic...

journal_title：Biochemical and biophysical research communications

authors： Chen X,Xu C,He H

更新日期：2019-09-03 00:00:00

abstract：:Exposure of mesangial cells to ionic Cd(2+) induces the proto-oncogene c-fos, while activating both Erk and stress-activated protein kinase (SAPK) MAP kinase pathways. While we have previously used a pharmacological inhibitor of Erk activation to implicate involvement of this pathway in the induction of c-fos by Cd(2+...

journal_title：Biochemical and biophysical research communications

authors： Ding W,Templeton DM

更新日期：2000-07-05 00:00:00

abstract：:The (1)H NMR spectrum of the perchloric acid extract of carp seminal plasma was heavily congested. It is demonstrated that proton-detected C,H chemical shift correlation spectroscopy (HSQC, HSQC-TOCSY) allows an unequivocal identification of proline, glutamate, taurine, and methionine sulfoxide, although several key p...

journal_title：Biochemical and biophysical research communications

authors： Kalic M,Lauterwein J,Büsselmann G,Kamp G

更新日期：2000-03-05 00:00:00

abstract：:Angiopoietin-2 (Ang-2) plays an important role in destabilizing vessels for angiogenesis, however its transcriptional regulation has not been determined. Here we isolated about 3.2kb of the 5' upstream of the human Ang-2 gene and further characterized the transcriptional regulation of Ang-2. We found 10 Ets binding si...

journal_title：Biochemical and biophysical research communications

authors： Hasegawa Y,Abe M,Yamazaki T,Niizeki O,Shiiba K,Sasaki I,Sato Y

更新日期：2004-03-26 00:00:00

abstract：:Human embryonic stem cells (hESC) are pluripotent, and can be directed to differentiate into different cell types for therapeutic applications. To expand hESCs, it is desirable to maintain hESC growth without differentiation. As hESC colonies grow, differentiated cells are often found at the periphery of the colonies,...

journal_title：Biochemical and biophysical research communications

authors： Lam H,Patel S,Wong J,Chu J,Lau A,Li S

更新日期：2008-08-08 00:00:00

abstract：:In vivo electroporation and hydrodynamics-based gene delivery were utilized to test the effect of leptin gene transfer on food intake, and body and fat weights of mice. Gene transfer of pVRmob by electroporation caused a significant reduction in body weight compared with the control counterpart (p<0.05), although a le...

journal_title：Biochemical and biophysical research communications

authors： Xiang L,Murai A,Sugahara K,Yasui A,Muramatsu T

更新日期：2003-08-01 00:00:00

abstract：:The causative agent of Lyme disease, Borrelia burgdorferi, requires shifts in gene expression to undergo its natural enzootic cycle between tick and vertebrate hosts. mRNA decay mechanisms play significant roles in governing gene expression in other bacteria, but are not yet characterized in B. burgdorferi. RNase III ...

journal_title：Biochemical and biophysical research communications

authors： Snow S,Bacon E,Bergeron J,Katzman D,Wilhelm A,Lewis O,Syangtan D,Calkins A,Archambault L,Anacker ML,Schlax PJ

更新日期：2020-08-20 00:00:00

abstract：:Zinc is an essential element for the biological system. However, excessive exogenous Zn2+ would disrupt cellular Zn2+ homeostasis and cause toxicity. In particular, Zinc salts or ZnO nanoparticles exposure could induce respiratory injury. Although previous studies have indicated that organelle damage (including mitoch...

journal_title：Biochemical and biophysical research communications

authors： Qin X,Zhang J,Wang B,Xu G,Zou Z

更新日期：2017-06-17 00:00:00

abstract：:We developed a modified method enabling stable MALDI-MS analysis and fluorescent detection of sialyl-compounds. The modification involved the amidation of sialic acid (Neu5Ac) at the position of the carboxyl group using the fluorescent reagent, 2-(2-pyridilamino)ethylamine (PAEA). In this study the following sialyl-co...

journal_title：Biochemical and biophysical research communications

authors： Endo S,Morita M,Ueno M,Maeda T,Terabayashi T

更新日期：2009-01-23 00:00:00

abstract：:Recently, circular RNAs (circRNAs) are identified as a novel class of noncoding RNAs playing important roles in human malignant tumors. However, the regulatory function of circRNA in lung adenocarcinoma (LUAD) is still largely unknown. Present study aimed to explore the role of circ_0006427 in LUAD progression. Firstl...

journal_title：Biochemical and biophysical research communications

authors： Yao Y,Hua Q,Zhou Y

更新日期：2019-01-01 00:00:00

abstract：:The compounds [Pt(IV)(dach)(9-methylguanine)2X2]2+ (X = Cl, OH) have been prepared and structurally characterized. Their existence shows that it is possible to accommodate two purine bases (in a cis configuration) and four other ligands around a Pt(IV) atom. The geometries of these complexes have very different orient...

journal_title：Biochemical and biophysical research communications

authors： Choi HK,Huang SK,Bau R

更新日期：1988-11-15 00:00:00

abstract：:Glypican 3 (GPC3), a GPI-anchored heparan sulfate proteoglycan, is expressed in the majority of hepatocellular carcinoma (HCC) tissues. Using MRL/lpr mice, we successfully generated a series of anti-GPC3 monoclonal antibodies (mAbs). GPC3 was partially cleaved between Arg358 and Ser359, generating a C-terminal 30-kDa ...

journal_title：Biochemical and biophysical research communications

authors： Nakano K,Orita T,Nezu J,Yoshino T,Ohizumi I,Sugimoto M,Furugaki K,Kinoshita Y,Ishiguro T,Hamakubo T,Kodama T,Aburatani H,Yamada-Okabe H,Tsuchiya M

更新日期：2009-01-09 00:00:00

abstract：:A trisaccharide (Glcalpha1-4Glcalpha1-6Glc) and a tetrasaccharide (Glcalpha1-4Glcalpha1-4Glcalpha1-6Glc) the structures of which are related to that of repeating unit of pullulan have been obtained, exploiting the transglycolytic activity of Aspergillus niger cyclodextrin glucanotransferase. Both products were obtaine...

journal_title：Biochemical and biophysical research communications

authors： Campa C,Vetere A,Gamini A,Donati I,Paoletti S

更新日期：2002-09-20 00:00:00

abstract：:Down syndrome (DS, Trisomy 21) is the most common genetic cause of delayed fetal brain development and postnatal intellectual disability. Although delayed fetal brain development might be involved in intellectual disability, no evidence of an association between these abnormal phenotypes has been shown. To identify mo...

journal_title：Biochemical and biophysical research communications

authors： Shimizu R,Ishihara K,Kawashita E,Sago H,Yamakawa K,Mizutani KI,Akiba S

更新日期：2021-01-08 00:00:00