Assignment of overlapping (1)H NMR signals in carp seminal plasma by proton-detected 2D C,H correlation spectroscopy.

abstract：:Conjugated linoleic acid (CLA), a group of minor fatty acids from ruminant origin, has long been recognized as a body fat lowering agent. Given the trans(t)10,cis(c)12-CLA well documented interference on lipolysis, we hypothesized for adipocytes altered permeation to glycerol when supplemented with this isomer. 3T3-L1...

journal_title：Biochemical and biophysical research communications

authors： Martins SV,Madeira A,Lopes PA,Pires VM,Alfaia CM,Prates JA,Moura T,Soveral G

更新日期：2015-03-06 00:00:00

abstract：:Although N-acetyl-L-aspartate (NAA) has been shown to be important to myelin synthesis and osmotic regulation, the biological rationale for the high levels of NAA found in the brain remains unknown. Here, a human astroglial cell line (STTG) was treated with NAA and stimulated with ionomycin, ionomycin/PMA, or IL-1beta...

journal_title：Biochemical and biophysical research communications

authors： Rael LT,Thomas GW,Bar-Or R,Craun ML,Bar-Or D

更新日期：2004-07-02 00:00:00

abstract：:Because of the growing importance of the Retinoid X Receptors (RXR alpha, beta and gamma) in the retinoid acid signalling pathway, we have prepared polyclonal and monoclonal antibodies directed against these proteins. For this purpose, either the whole mouse RXR alpha protein expressed in E.Coli, or synthetic peptides...

journal_title：Biochemical and biophysical research communications

authors： Rochette-Egly C,Lutz Y,Pfister V,Heyberger S,Scheuer I,Chambon P,Gaub MP

更新日期：1994-10-28 00:00:00

abstract：:One of the most important issues in stem cell research is to understand the regulatory mechanisms responsible for their differentiation. An extensive understanding of mechanism underlying the process of differentiation is crucial in order to prompt stem cells to perform a particular function after differentiation. To ...

journal_title：Biochemical and biophysical research communications

authors： Feng Z,Srivastava AS,Mishra R,Carrier E

更新日期：2004-11-26 00:00:00

abstract：:HERC2 is a giant protein with E3 ubiquitin ligase activity and other known and suspected functions. Mutations of HERC2 are implicated in the pathogenesis of various cancers and result in severe neurological conditions in Herc2-mutant mice. Recently, a pleotropic autosomal recessive HERC2-associated syndrome of intelle...

journal_title：Biochemical and biophysical research communications

authors： Abraham JR,Barnard J,Wang H,Noritz GH,Yeganeh M,Buhas D,Natowicz MR

更新日期：2019-04-30 00:00:00

abstract：:The carnitine/acylcarnitine carrier from rat liver mitochondria was overexpressed in Escherichia coli. The expressed protein, recovered as inclusion bodies, was solubilized with sarkosyl and purified by Sephadex G-200 and celite chromatography. A yield of 15 mg of purified transport protein per liter of cell culture w...

journal_title：Biochemical and biophysical research communications

authors： Indiveri C,Iacobazzi V,Giangregorio N,Palmieri F

更新日期：1998-08-28 00:00:00

abstract：:The upstream activating region that controls cellulose-induced expression of the glucose-repressible cellobiohydrolase I gene (UARcb1) of the filamentous fungus Trichoderma reesei is shown to mediate transcription and glucose repression of a reporter gene in Saccharomyces cerevisiae, a unicellular microorganism that l...

journal_title：Biochemical and biophysical research communications

authors： Carraro DM,Ferreira Júnior JR,Schumacher R,Pereira GG,Hollenberg CP,El-Dorry H

更新日期：1998-12-18 00:00:00

abstract：:The Sec23p-Sec24p complex is a component of coat protein II-coated vesicles involved in protein export from the endoplasmic reticulum. We previously identified a novel Sec23p-interacting protein, p125, which consists of 1000 amino acids and comprises a proline-rich region and a phospholipase A(1) homology region. p125...

journal_title：Biochemical and biophysical research communications

authors： Mizoguchi T,Nakajima K,Hatsuzawa K,Nagahama M,Hauri HP,Tagaya M,Tani K

更新日期：2000-12-09 00:00:00

abstract：:Autophagy is a physiologically regulated and evolutionary conserved process that plays a critical role in degradation of cytoplasmic proteins and other macromolecules within the lysosomes. Beclin-1, the mammalian orthologue of yeast Atg6, is an important mediator of autophagy that has been studied in many human cancer...

journal_title：Biochemical and biophysical research communications

authors： Kapoor V,Paliwal D,Baskar Singh S,Mohanti BK,Das SN

更新日期：2012-06-15 00:00:00

abstract：:Sphingosine 1-phosphate (S1P) has recently been described to induce antimycobacterial activity. The present study analyses the role played by S1P in antigen presentation of monocytes and in the next activation of Mycobacterium tuberculosis (MTB)-specific CD4+ T cell response. Results reported herein show that S1P stim...

journal_title：Biochemical and biophysical research communications

authors： Santucci MB,Greco E,De Spirito M,Arcovito G,De Angelis G,Cauda R,Fraziano M

更新日期：2007-09-28 00:00:00

abstract：:Effects of the imidazoline compound RX871024 on cytosolic free Ca(2+) concentration ([Ca(2+)]i) and insulin secretion in pancreatic beta-cells from SUR1 deficient mice have been studied. In beta-cells from wild-type mice RX871024 increased [Ca(2+)]i by blocking ATP-dependent K(+)-current (K(ATP)) and inducing membrane...

journal_title：Biochemical and biophysical research communications

authors： Efanov AM,Høy M,Bränström R,Zaitsev SV,Magnuson MA,Efendic S,Gromada J,Berggren PO

更新日期：2001-06-22 00:00:00

abstract：:Human bone marrow mesenchymal stem cells (hMSCs) are a promising source for clinical stem cell transplantation. However, telomere regulation mechanisms, as one of the possible major mechanisms by which hMSCs sustain their stem cell characteristics, remain unknown. We isolated hMSCs by plastic adhesion and characterize...

journal_title：Biochemical and biophysical research communications

authors： Zhao YM,Li JY,Lan JP,Lai XY,Luo Y,Sun J,Yu J,Zhu YY,Zeng FF,Zhou Q,Huang H

更新日期：2008-05-16 00:00:00

abstract：:The Rex-family repressors sense redox levels by alternative binding to NADH or NAD(+). RSP is the homologue of Rex in Thermoanaerobacter ethanolicus JW200(T) and regulates ethanol fermentation in this obligate anaerobe. The dimeric repressor binds to DNA by an open conformation. The crystal structure of RSP/α-NAD(+) c...

journal_title：Biochemical and biophysical research communications

authors： Zheng Y,Ko TP,Yang Y,Shao W,Guo RT

更新日期：2015-01-16 00:00:00

abstract：:In this report we demonstrate formation of a triplex structure by an antiparallel RNA oligonucleotide corresponding to the 21 bp polypurine-pyrimidine stretch from -141 to -162 of the rat alpha 1(I) procollagen promoter with a Kd of 0.1-0.2 microM. The formation of triplexes by the triplex forming oligoribonucleotide ...

journal_title：Biochemical and biophysical research communications

authors： Ririe SS,Guntaka RV

更新日期：1998-08-10 00:00:00

abstract：:Transgenic mice expressing nuclear sterol regulatory element-binding protein-1a under the control of the insulin promoter were generated to determine the role of SREBP-1a in pancreatic beta-cells. Only low expressors could be established, which exhibited mild hyperglycemia, impaired glucose tolerance, and reduced plas...

journal_title：Biochemical and biophysical research communications

authors： Iwasaki Y,Iwasaki H,Yatoh S,Ishikawa M,Kato T,Matsuzaka T,Nakagawa Y,Yahagi N,Kobayashi K,Takahashi A,Suzuki H,Yamada N,Shimano H

更新日期：2009-01-16 00:00:00

abstract：:Down regulation of prolactin (PRL) receptors resulting from i.v. infusion of oPRL or human growth hormone (hGH) into female virgin rats was demonstrated. A decrease of over 85% in the number of free receptors was achieved within 15 - 30 min using infusion of oPRL or hGH at 25 micrograms/h and remained at this level un...

journal_title：Biochemical and biophysical research communications

authors： Barash I,Madar Z,Gertler A

更新日期：1983-10-31 00:00:00

abstract：:Previous genetic evidence led to the conclusion that proteinase B of yeast was not involved in the function of chitin synthetase 1 (Chs1), based on the demonstration of normal septum formation, cell division and chitin deposition in mutants devoid of the proteinase (Zubenko, G.S., Mitchell, A.P., and Jones, E.W. (1979...

journal_title：Biochemical and biophysical research communications

authors： Silverman SJ,Shaw JA,Cabib E

更新日期：1991-01-15 00:00:00

abstract：:Treatment of a human promyelocytic leukemia cell line (HL-60) with etoposide for 3-4 hrs produced an extensive degradation of DNA. Agarose gel electrophoresis showed DNA fragmentation in a nucleosomal ladder pattern. Simultaneous addition of zinc ion (ZnSO4, 1 mM) inhibited DNA fragmentation, although the amount of DN...

journal_title：Biochemical and biophysical research communications

authors： Shimizu T,Kubota M,Tanizawa A,Sano H,Kasai Y,Hashimoto H,Akiyama Y,Mikawa H

更新日期：1990-06-29 00:00:00

abstract：:1,25-Dihydroxyvitamin D(3) [1,25(OH)(2)D(3)] initiates the hydrolysis of sphingomyelin in ROS 17/2.8 osteosarcoma cells with the resultant generation of cell-associated ceramide. Increases in ceramide levels were detectable at 15 min and maximal one hour after exposure of cells to 1,25(OH)(2)D(3). Neither 1,25(OH)(2)D...

journal_title：Biochemical and biophysical research communications

authors： Liu R,Xu Y,Farach-Carson MC,Vogel JJ,Karin NJ

更新日期：2000-06-24 00:00:00

abstract：:We here isolated and characterized a cDNA clone encoding 40-kDa heat-shock protein hsp40 from cDNA expression library of human placenta by immunoscreening with anti-hsp40 antibody. N-terminal amino acid sequence (48 residues) deduced from the base sequence was completely identical to that of hsp40 purified from HeLa c...

journal_title：Biochemical and biophysical research communications

authors： Ohtsuka K

更新日期：1993-11-30 00:00:00

abstract：:The late endosomal marker Rab7 has been long believed to be absent from the phagosome containing Mycobacterium tuberculosis (M.tb) in macrophage, but the detail kinetics remains elusive. Here, we found that Rab7 is transiently recruited to and subsequently released from M.tb phagosomes. For further understanding of th...

journal_title：Biochemical and biophysical research communications

authors： Seto S,Matsumoto S,Ohta I,Tsujimura K,Koide Y

更新日期：2009-09-18 00:00:00

abstract：:We have previously shown that mammalian brain crude extracts contained two classes of stable microtubules: "cold stable" and "super-stable" microtubules. We now find that both species are generated by a single protein factor (STOP protein) in a dose dependent manner. These results show that STOP protein action can be ...

journal_title：Biochemical and biophysical research communications

authors： Job D,Rauch CT,Margolis RL

更新日期：1987-10-14 00:00:00

abstract：:Isoprenoids comprise a diverse group of natural products with a broad range of metabolic functions. Isoprenoids are synthesized from prenyl pyrophosphates by prenyltransferases that catalyze the isoprenoid chain-elongation process to different chain lengths. We hereby present the crystal structure of geranylgeranyl py...

journal_title：Biochemical and biophysical research communications

authors： Kim S,Kim EJ,Park JB,Kim SW,Kim KJ

更新日期：2019-10-20 00:00:00

abstract：:The Sir proteins, namely Sir2, 3 and 4, have roles related to heterochromatin, but genome-wide studies have revealed their presence at many euchromatic loci, although the functional meaning of this is still not clear. Nhp6a is an abundant HMG-like protein in yeast, which has a role in transcription by modulating chrom...

journal_title：Biochemical and biophysical research communications

authors： Ciuffetta A,Salerno D,Camilloni G,Venditti S

更新日期：2015-05-22 00:00:00

abstract：:Carbohydrate response element binding protein (ChREBP) is responsible for conversion of dietary carbohydrate to storage fat in liver by coordinating expression of the enzymes that channel glycolytic pyruvate into lipogenesis. The activation of ChREBP in response to high glucose is nuclear localization and transcriptio...

journal_title：Biochemical and biophysical research communications

authors： Fukasawa M,Ge Q,Wynn RM,Ishii S,Uyeda K

更新日期：2010-01-08 00:00:00

abstract：BACKGROUND/AIMS:We have previously reported that the application of sphingomyelin-based liposomes (SM-L) to a three-dimensional cultured skin model increase the content of ceramides NS, NP, AS and AP. However, the mechanism responsible for these increased ceramide levels was not identified. METHODS:SM-L and sphingomye...

journal_title：Biochemical and biophysical research communications

authors： Itaya Y,Tokudome Y

更新日期：2016-04-22 00:00:00

abstract：:A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using "ink-jet" protein arraying technology. The solid-phase affinity substrate termed the "ViriChip" was used in the immunocap...

journal_title：Biochemical and biophysical research communications

authors： Nettikadan SR,Johnson JC,Mosher C,Henderson E

更新日期：2003-11-14 00:00:00

abstract：:(6R)5,6,7,8 tetrahydrobiopterin (6-BH4) is an important cofactor in the regulation of melanogenesis in melanocytes, where it controls: (a) the supply of L-tyrosine from L-phenylalanine via phenylalanine hydroxylase, and (b) regulates directly dopaquinone formation from L-tyrosine via tyrosinase. 6-BH4 undergoes redox-...

journal_title：Biochemical and biophysical research communications

authors： Schallreuter KU,Büttner G,Pittelkow MR,Wood JM,Swanson NN,Körner C

更新日期：1994-10-14 00:00:00

abstract：:Tissue-engineered grafts for tissue regeneration include either mature or progenitor cells seeded onto biomatrices that provide shape and support for developing tissue. Popular biomaterials used in orthopaedic surgery include collagen type I, hyaluronic acid, hydroxyapatite, and polylactic polyglycolic acid (PLGA). Bi...

journal_title：Biochemical and biophysical research communications

authors： Carlson GA,Dragoo JL,Samimi B,Bruckner DA,Bernard GW,Hedrick M,Benhaim P

更新日期：2004-08-20 00:00:00

abstract：:Caspase-independent, non-apoptotic cell death is an important therapeutic target in myocardial ischemia. Leptin, an adipose-derived hormone, is known to exhibit cytoprotective effects on the ischemic heart, but the mechanisms are poorly understood. In this research, we found that pretreatment of leptin strongly suppre...

journal_title：Biochemical and biophysical research communications

authors： Takatani-Nakase T,Takahashi K

更新日期：2015-07-17 00:00:00