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Virus particle detection by solid phase immunocapture and atomic force microscopy.

Abstract：

:A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using "ink-jet" protein arraying technology. The solid-phase affinity substrate termed the "ViriChip" was used in the immunocapture of bacteriophage fd, canine parvoviruses, and coxsackieviruses and analyzed by AFM. Immunocapture was found to be antibody-specific with a sensitivity of 10(8)pfu/ml in 30min. Virus binding was found to be linear for concentration between 10(8) and 10(10)pfu/ml and did not reach saturation through 4h.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nettikadan SR,Johnson JC,Mosher C,Henderson E

doi

10.1016/j.bbrc.2003.10.022

subject

Has Abstract

pub_date

2003-11-14 00:00:00

pages

540-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X03020795

journal_volume

311

pub_type

杂志文章

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