当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis.

Structural analysis of a putative SAM-dependent methyltransferase, YtqB, from Bacillus subtilis.

Abstract：

:S-adenosyl-L-methionine (SAM)-dependent methyltransferases (MTases) methylate diverse biological molecules using a SAM cofactor. The ytqB gene of Bacillus subtilis encodes a putative MTase and its biological function has never been characterized. To reveal the structural features and the cofactor binding mode of YtqB, we have determined the crystal structures of YtqB alone and in complex with its cofactor, SAM, at 1.9 Å and 2.2 Å resolutions, respectively. YtqB folds into a β-sheet sandwiched by two α-helical layers, and assembles into a dimeric form. Each YtqB monomer contains one SAM binding site, which shapes SAM into a slightly curved conformation and exposes the reactive methyl group of SAM potentially to a substrate. Our comparative structural analysis of YtqB and its homologues indicates that YtqB is a SAM-dependent class I MTase, and provides insights into the substrate binding site of YtqB.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Park SC,Song WS,Yoon SI

doi

10.1016/j.bbrc.2014.03.026

subject

Has Abstract

pub_date

2014-04-18 00:00:00

pages

921-6

issue

4

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(14)00460-4

journal_volume

446

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Insulin-dependent phosphatidylinositol 3'-kinase activity co-precipitates with insulin receptor in human circulating mononuclear cells.

    abstract：:In order to establish a working cellular model for the study of post-receptor signaling events, insulin-dependent phosphatidylinositol 3' (PtdIns 3')-kinase activity was investigated in circulating mononuclear cells from normal subjects. The p85 alpha regulatory subunit of PtdIns 3'-kinase co-precipitated with activat...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1494

    authors： Renard E,Grigorescu F,Lavabre C,Kahn CR

    更新日期：1995-04-06 00:00:00

  • Active sites of two orthologous cytochromes P450 2E1: differences revealed by spectroscopic methods.

    abstract：:Cytochromes P450 2E1 of human and minipig origin were examined by absorption spectroscopy under high hydrostatic pressure and by resonance Raman spectroscopy. Human enzyme tends to denature to the P420 form more easily than the minipig form; moreover, the apparent compressibility of the heme active site (as judged fro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.063

    authors： Anzenbacherova E,Hudecek J,Murgida D,Hildebrandt P,Marchal S,Lange R,Anzenbacher P

    更新日期：2005-12-09 00:00:00

  • Phylogenetic origin and sequence features of MreB from the wall-less swimming bacteria Spiroplasma.

    abstract：:Spiroplasma are wall-less bacteria which belong to the phylum Tenericutes that evolved from Firmicutes including Bacillus subtilis. Spiroplasma swim by a mechanism unrelated to widespread bacterial motilities, such as flagellar motility, and caused by helicity switching with kinks traveling along the helical cell body...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.09.060

    authors： Takahashi D,Fujiwara I,Miyata M

    更新日期：2020-12-17 00:00:00

  • Oligomerization of an archaeal group II chaperonin is mediated by N-terminal salt bridges.

    abstract：:Group II chaperonins (Cpns) are essential mediators of cellular protein folding in eukaryotes and archaea. They consist of two back-to-back rings forming symmetrical cavities in which non-native substrates undergo appropriate folding, but the primary structural basis for the double ring formation remains unclear. To a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.08.112

    authors： Luo H,Zhang P,Robb FT

    更新日期：2011-09-23 00:00:00

  • β-cell serotonin production is associated with female sex, old age, and diabetes-free condition.

    abstract：:Serotonin is known to be present in pancreatic β-cells and to play several physiological roles, including insulin secretion, β-cell proliferation, and paracrine inhibition of α-cells. However, the serotonin production of different cell lines and islets has not been compared based on age, sex, and diabetes related cond...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.09.130

    authors： Kim YG,Moon JH,Kim K,Kim H,Kim J,Jeong JS,Lee J,Kang S,Park JS,Kim H

    更新日期：2017-11-25 00:00:00

  • Identification and functional analysis of consensus androgen response elements in human prostate cancer cells.

    abstract：:Androgen receptor (AR) recognizes and binds to 15-bp palindromic androgen response element (ARE) sequences with high affinity in vitro, which consist of two hexameric half-sites arranged as inverted repeats with a 3-bp spacer. Although a few near-consensus ARE sequences have been actually identified in the transcripti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.10.174

    authors： Horie-Inoue K,Bono H,Okazaki Y,Inoue S

    更新日期：2004-12-24 00:00:00

  • Disruption of TGF-β signaling in smooth muscle cell prevents elastase-induced abdominal aortic aneurysm.

    abstract：:Transforming growth factor-β (TGF-β) signaling has been significantly implicated in the pathogenesis of aneurysm, prominently the initiation and progression of abdominal aortic aneurysm (AAA). Vascular smooth muscle cell (SMC) is the principal resident cell in aortic wall and is essential for its structure and functio...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.10.053

    authors： Gao F,Chambon P,Offermanns S,Tellides G,Kong W,Zhang X,Li W

    更新日期：2014-11-07 00:00:00

  • Chinese hamster ovary cells resistant to okadaic acid express a multidrug resistant phenotype.

    abstract：:Two Chinese hamster ovary cell clones resistant to okadaic acid (OA) were isolated. The OA-resistance was associated with resistance to colchicine, Vinca alkaloids and inhibitors of DNA topoisomerase (topo) II. Drug accumulation assays showed that the intracellular levels of OA, vinblastine and vincristine, but not th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2311

    authors： Tohda H,Yasui A,Yasumoto T,Nakayasu M,Shima H,Nagao M,Sugimura T

    更新日期：1994-09-15 00:00:00

  • Demonstration of antigenic similarities and variations in excretory/secretory antigens of Toxoplasma gondii.

    abstract：:C57BL/6 mice were orally infected with different doses of cysts of ME49 strain of Toxoplasma gondii to produce groups of acutely and chronically infected mice. Sera were obtained at different periods post-infection. SDS-PAGE was ran with excretory/secretory antigens of ME49 and RH strains of T. gondii, followed by Wes...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81491-3

    authors： Rahmah N,Anuar AK

    更新日期：1992-08-31 00:00:00

  • Biochemical evidence for the long-tail form (A beta 1-42/43) of amyloid beta protein as a seed molecule in cerebral deposits of Alzheimer's disease.

    abstract：:We measured the amounts of total A beta, A beta 1-40 and A beta 1-42/43 in brain tissues using a newly developed ELISA assay and found that the amounts of insoluble A beta 1-42/43 and insoluble A beta 1-40 were linearly related to the amount of A beta deposits or total insoluble A beta at their lower and higher concen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2740

    authors： Tamaoka A,Kondo T,Odaka A,Sahara N,Sawamura N,Ozawa K,Suzuki N,Shoji S,Mori H

    更新日期：1994-11-30 00:00:00

  • Endothelin action: Inhibition by a protein kinase C inhibitor and involvement of phosphoinositols.

    abstract：:Endothelin tightly bound to rabbit aortic strips and caused a prolonged vasoconstriction both in the presence and absence of extracellular Ca2+, although only partial constriction (20-30%) developed in the latter case, indicating that its action may not be limited to the opening of a calcium channel. The endothelin-in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(89)80193-7

    authors： Sugiura M,Inagami T,Hare GM,Johns JA

    更新日期：1989-01-16 00:00:00

  • alpha-Difluoromethylornithine does not bind to ornithine decarboxylase-antizyme complex.

    abstract：:alpha-Difluoromethylornithine (DFMO) has been widely used for determining the amounts of ornithine decarboxylase (ODC) in cells. However, we have now found that DFMO does not react with ODC-antizyme complex, indicating that the amounts of radioactive DFMO bound to the protein may not reflect the amounts of ODC in crud...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)90338-4

    authors： Kitani T,Fujisawa H

    更新日期：1986-06-30 00:00:00

  • N-methyl-N-nitrosourea evidences cell cycle associated transient sequences in hepatic replicating DNA.

    abstract：:Experiments of molecular hybridization were carried out with the aim of singling out DNA sequences which might be important in the hepatocarcinogenic action of N-methyl-N-nitrosourea. Results were obtained suggesting the synthesis of transitory DNA sequences during compensatory cell proliferation. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1367

    authors： Kanduc D

    更新日期：1995-03-17 00:00:00

  • Antibody-induced activation of the epidermal growth factor receptor tyrosine kinase requires the presence of detergent.

    abstract：:Activation of the epidermal growth factor receptor (EGF-R) tyrosine kinase was investigated in membrane preparations as well as intact A431 cells, using anti-EGF-R antibodies directed against extra- and intracellular receptor domains. In vitro assay conditions were mimicked on whole cells by a mild detergent treatment...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91228-k

    authors： Spaargaren M,Defize LH,de Laat SW,Boonstra J

    更新日期：1990-09-14 00:00:00

  • RNA polymerase II mediated transcription from the polymerase III promoters in short hairpin RNA expression vector.

    abstract：:RNA polymerase III promoters of human ribonuclease P RNA component H1, human U6, and mouse U6 small nuclear RNA genes are commonly used in short hairpin RNA (shRNA) expression vectors due their precise initiation and termination sites. During transient transfection of shRNA vectors, we observed that H1 or U6 promoters...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.037

    authors： Rumi M,Ishihara S,Aziz M,Kazumori H,Ishimura N,Yuki T,Kadota C,Kadowaki Y,Kinoshita Y

    更新日期：2006-01-13 00:00:00

  • BTF3 promotes stemness and inhibits TypeⅠInterferon signaling pathway in triple-negative breast cancer.

    abstract：:Triple-negative breast cancer (TNBC) is a major challenge in clinical practice due to its aggressiveness and lack of targeted treatment. Cancer stem-like traits contribute to tumorigenesis and immune privilege of TNBC. However, the relationship of stemness and immunosurveillance remains unclear. Here, we demonstrate t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.12.060

    authors： Wang H,Gao L,Qi M,Su P,Xiong X,Zhao J,Hu J,Han B

    更新日期：2021-01-22 00:00:00

  • The voltage-dependent sodium channel is co-localized with the acetylcholine receptor at the vertebrate neuromuscular junction.

    abstract：:Isolated motor endplates from mouse intercostal muscles can be obtained after subcellular fractionation. On these motor endplates, localization of the nicotinic receptor and of the voltage-dependent Na+ channel coincides as demonstrated by double labeling with rhodamine alpha-bungarotoxin and a specific anti-Na+ chann...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80098-5

    authors： Dreyfus P,Rieger F,Murawsky M,Garcia L,Lombet A,Fosset M,Pauron D,Barhanin J,Lazdunski M

    更新日期：1986-08-29 00:00:00

  • NF-kappaB and ZBP-89 regulate MMP-3 expression via a polymorphic site in the promoter.

    abstract：:A 5T/6T polymorphism in the human MMP-3 promoter affects gene expression and impacts the risk and/or severity of various pathological conditions. Chromatin immunoprecipitation (ChIP) in human fibroblasts homozygous for the 6T site demonstrate that it is bound by NF-kappaB and ZBP-89 transcription factors in its native...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.03.002

    authors： Borghaei RC,Gorski G,Javadi M,Mariah Chambers.

    更新日期：2009-05-01 00:00:00

  • A new member of the sorting nexin family interacts with the C-terminus of P-selectin.

    abstract：:P-selectin is a cell adhesion molecule found in platelets and endothelial cells mediating binding of leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. After rapid internalisation P-selectin recycles or is degraded. The 35 amino acid cytoplasmic domain of P-selec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.4467

    authors： Florian V,Schlüter T,Bohnensack R

    更新日期：2001-03-09 00:00:00

  • Mermaid, a family of short interspersed repetitive elements, is useful for zebrafish genome mapping.

    abstract：:A family of short interspersed repetitive elements (SINEs), designated mermaid, is present in the genomes of fish, amphibian and primates, but absent in the mouse genome. We have demonstrated that the sequences of the mermaid family are highly polymorphic in the zebrafish genome as in the human genome. We have also sh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0386

    authors： Shimoda N,Chevrette M,Ekker M,Kikuchi Y,Hotta Y,Okamoto H

    更新日期：1996-03-07 00:00:00

  • Biosynthesis of 12-oxo-10,15(Z)-phytodienoic acid: identification of an allene oxide cyclase.

    abstract：:Incubation of 13(S)-hydroperoxy-9(Z),11(E),15(Z)-octadecatrienoic acid with corn (Zea mays L.) hydroperoxide dehydrase led to the formation of an unstable allene oxide derivative, 12,13(S)-epoxy-9(Z),11,15(Z)-octadecatrienoic acid. Further conversion of the allene oxide yielded two major products, i.e. alpha-ketol 12-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80876-3

    authors： Hamberg M

    更新日期：1988-10-14 00:00:00

  • Detection of retinoid X receptors using specific monoclonal and polyclonal antibodies.

    abstract：:Because of the growing importance of the Retinoid X Receptors (RXR alpha, beta and gamma) in the retinoid acid signalling pathway, we have prepared polyclonal and monoclonal antibodies directed against these proteins. For this purpose, either the whole mouse RXR alpha protein expressed in E.Coli, or synthetic peptides...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2491

    authors： Rochette-Egly C,Lutz Y,Pfister V,Heyberger S,Scheuer I,Chambon P,Gaub MP

    更新日期：1994-10-28 00:00:00

  • Tumor necrosis factor upregulates interleukin-4 receptors on murine sarcoma cells.

    abstract：:We have previously shown that murine solid tumor cells express high affinity IL-4 receptors (IL-4R) which are internalized after binding to ligand. In the present study, we have examined the regulation of IL-4R by TNF. We demonstrate that TNF upregulated the expression of IL-4R on murine MCA-106 sarcoma cells. Maximum...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2636

    authors： Puri RK,Leland P

    更新日期：1993-12-30 00:00:00

  • Differentiative action of K252a on protein kinase C and a calcium-unresponsive, phorbol ester/phospholipid-activated protein kinase.

    abstract：:The Triton X-100 extract of the particulate fraction of porcine spleen contains a protein kinase which can be activated by phospholipid and the phorbol ester TPA but does not respond to phospholipid and calcium. The partially purified kinase has a molecular weight of 76 kDa (p76-kinase) and hence is somewhat smaller t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91765-8

    authors： Gschwendt M,Leibersperger H,Marks F

    更新日期：1989-11-15 00:00:00

  • Mouse ARF-related protein 1: genomic organization and analysis of its promoter.

    abstract：:ARF-related protein 1 (ARFRP1) is a membrane-associated GTPase which inhibits the ARF/Sec7-dependent activation of phospholipase D. We have recently shown that deletion of Arfrp1 in mice results in increased apoptosis of mesodermal cells during gastrulation, leading to early embryonic lethality. Here we describe the o...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6628

    authors： Mueller AG,Joost HG,Schürmann A

    更新日期：2002-03-22 00:00:00

  • In vitro transfer of phosphatidylcholines and their ether analogs by a human and rat plasma exchange factor.

    abstract：:The phospholipid transfer properties of human and rat plasma have been studied using radiolabeled phosphatidylcholines (PCs) and diether PCs as well as a series of fluorescent PCs. The PC transfer activities of human and rat lipoprotein deficient sera are similar. Lipoprotein deficient rat serum transfers PC at a rate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80270-3

    authors： Pownall HJ,Hickson DL,Gotto AM Jr,Massey JB

    更新日期：1984-03-15 00:00:00

  • Sulfur dioxide upregulates the inhibited endogenous hydrogen sulfide pathway in rats with pulmonary hypertension induced by high pulmonary blood flow.

    abstract：:Pulmonary hypertension (PH) is an important pathophysiological process in the development of many diseases. However, the mechanism responsible for the development of PH remains unknown. The objective of the study was to explore the possible impact of sulfur dioxide (SO2) on the endogenous hydrogen sulfide (H2S) pathwa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.03.014

    authors： Luo L,Liu D,Tang C,Du J,Liu AD,Holmberg L,Jin H

    更新日期：2013-04-19 00:00:00

  • Plk1 is negatively regulated by RNF8.

    abstract：:RNF8 is a nuclear protein having an N-terminal forkhead-associated (FHA) domain and a C-terminal RING-finger (RF) domain. Depletion of RNF8 caused cell growth inhibition and cell cycle arrest at not only S but also G2/M phases. In addition, cell death was frequently observed in RNF8-depleted cells. Analyses of time-la...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.05.104

    authors： Yoshioka T,Kimura M,Saio M,Era S,Okano Y

    更新日期：2011-06-24 00:00:00

  • Suppression of NADPH oxidase attenuates hypoxia-induced dysfunctions of endothelial progenitor cells.

    abstract：:NADPH oxidases (NOX) - derived reactive oxygen species (ROS) contribute to oxidative injury in hypoxia-induced pulmonary arterial hypertension. This study aims to evaluate the status of NOX in endothelial progenitor cells (EPCs) under hypoxic condition and to determine whether NOX inhibitors could attenuate hypoxia-in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.11.161

    authors： Liu B,Ren KD,Peng JJ,Li T,Luo XJ,Fan C,Yang JF,Peng J

    更新日期：2017-01-22 00:00:00

  • Synergistic potentiation of thiazolidinedione-induced ST 13 preadipocyte differentiation by RAR synergists.

    abstract：:Peroxisome proliferator-activated receptor gamma (PPAR gamma) belongs to a nuclear receptor super family that functions as a master regulator of adipocyte differentiation. PPAR gamma binds its DNA response element together with a partner, retinoid X receptor (RXR), in fat cells. Five RXR ligands (HX600, HX630, DA022, ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.4172

    authors： Sato M,Yajima Y,Kawashima S,Tanaka K,Kagechika H

    更新日期：2001-01-26 00:00:00