Enzymatic synthesis and characterization of oligosaccharides structurally related to the repeating unit of Pullulan.

Abstract:

:A trisaccharide (Glcalpha1-4Glcalpha1-6Glc) and a tetrasaccharide (Glcalpha1-4Glcalpha1-4Glcalpha1-6Glc) the structures of which are related to that of repeating unit of pullulan have been obtained, exploiting the transglycolytic activity of Aspergillus niger cyclodextrin glucanotransferase. Both products were obtained in one-pot reaction using as a donor the alpha-cyclodextrin and as an acceptor the disaccharide isomaltose. The regioselectivity of the reaction was 85% for the tetrasaccharide and 80% for the trisaccharide. The yield of reaction resulted to be 42% for the synthesis of trisaccharide and 25% for that of tetrasaccharide. Purification of products was performed by size exclusion chromatography and by semipreparative reverse phase HPLC after reversible derivatization with 2-aminopyridine. Structural characterization was performed by capillary electrophoresis, ion-spray mass spectrometry, and by 13C-NMR spectroscopy. A comparison of these results with those obtained by using alpha-D-glucosidase, which had been effective for the synthesis of the disaccharide isomaltose, is reported.

authors

Campa C,Vetere A,Gamini A,Donati I,Paoletti S

doi

10.1016/s0006-291x(02)02213-1

subject

Has Abstract

pub_date

2002-09-20 00:00:00

pages

382-9

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006291X02022131

journal_volume

297

pub_type

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