Green tea polyphenols: novel and potent inhibitors of squalene epoxidase.

abstract：:Genomic fragments containing coding sequences of the human liver phosphofructokinase (PFKL) were used to screen cDNA libraries and several clones corresponding to PFKL were isolated. Three overlapping cDNA clones spanning the entire coding region were stitched together yielding the plasmid pG-cPFKL3.0 containing a 3Kb...

journal_title：Biochemical and biophysical research communications

authors： Levanon D,Danciger E,Dafni N,Groner Y

更新日期：1987-09-30 00:00:00

abstract：:To elucidate the role of ornithine decarboxylase (ODC) in cancer cell invasion, we have compared the invasiveness of mouse mammary carcinoma, FM3A and its variant cell line, EXOD, which overproduces ODC. We have found that EXOD cells showed about 5.6-fold more invasiveness through a reconstituted basement membrane (Ma...

journal_title：Biochemical and biophysical research communications

authors： Kubota S,Yamada T,Kamei S,Seyama Y

更新日期：1995-03-28 00:00:00

abstract：:Osteoclasts play an important role in bone remodeling. The inflammatory cytokine IL-17A could modulate the RANKL-induced osteoclastogenesis by regulating the autophagic activity. It is well accepted that protective autophagy has an anti-apoptotic effect. It is necessary to elucidate whether IL-17A can influence the ap...

journal_title：Biochemical and biophysical research communications

authors： Xue Y,Liang Z,Fu X,Wang T,Xie Q,Ke D

更新日期：2019-01-22 00:00:00

abstract：:KCNQ1 plays important roles in the cardiac action potential and consists of an N-terminal domain, a voltage-sensor domain, a pore domain and a C-terminal domain. KCNQ1 is a voltage-gated potassium channel and its channel activity is regulated by membrane potentials. The linker between transmembrane helices 4 and 5 (S4...

journal_title：Biochemical and biophysical research communications

authors： Gayen S,Li Q,Kang C

更新日期：2015-01-02 00:00:00

abstract：:Examination of human follicular fluid revealed the presence of polychlorinated dibenzodioxins (PCDDs) and dibenzofurans (PCDFs) at concentrations of approximately 1 pg/ml (0.01 pg TEQ/ml). To study their possible action, two-cell mouse embryos were cultured in the presence of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)...

journal_title：Biochemical and biophysical research communications

authors： Tsutsumi O,Uechi H,Sone H,Yonemoto J,Takai Y,Momoeda M,Tohyama C,Hashimoto S,Morita M,Taketani Y

更新日期：1998-09-18 00:00:00

abstract：:In RNA, function follows form. Mapping the surface of RNA molecules with chemical and enzymatic probes has revealed invaluable information about structure and folding. Hydroxyl radicals ((·)OH) map the surface of nucleic acids by cutting the backbone where it is accessible to solvent. Recent studies showed that a micr...

journal_title：Biochemical and biophysical research communications

authors： Schlatterer JC,Wieder MS,Jones CD,Pollack L,Brenowitz M

更新日期：2012-08-24 00:00:00

abstract：:The acidic leucine-rich nuclear phosphoprotein 32B (ANP32B) is a member of a conserved superfamily of nuclear proteins whose functions are largely unknown. In our previous work, ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. In this work, ...

journal_title：Biochemical and biophysical research communications

authors： Yu Y,Shen SM,Zhang FF,Wu ZX,Han B,Wang LS

更新日期：2012-07-13 00:00:00

abstract：:We have obtained the optimized geometrical structure of 2-(1-carboxy-1-hydroxyethyl)-3,4-dimethylthiazolium dipolar ion and investigated its geometric and electric changes during decarboxylation process by the MNDO-PM3 method, a molecular orbital method. The salient features of the optimized structure are that the dih...

journal_title：Biochemical and biophysical research communications

authors： Nakajima Y,Sakagishi Y,Shiibashi M,Suzuki Y,Kato H

更新日期：1993-08-31 00:00:00

abstract：:There are data suggesting that HMG1 protein may be involved in DNA replication. Recently we have found that only the acetylated form of the protein generates tetramers, stimulates the activity of DNA polymerase alpha (EC 2.7.7.7) (with activated DNA as a template) and forms a specific complex with it. This paper compa...

journal_title：Biochemical and biophysical research communications

authors： Dimov SI,Alexandrova EA,Beltchev BG

更新日期：1990-01-30 00:00:00

abstract：:Signaling and desensitization of G protein-coupled receptor are intimately related, and measuring them separately requires certain parameters that represent desensitization independently of signaling. In this study, we tested whether desensitization requires signaling in three different receptors, beta2-adrenergic rec...

journal_title：Biochemical and biophysical research communications

authors： Barak LS,Gilchrist J,Becker JM,Kim KM

更新日期：2006-01-13 00:00:00

abstract：:Triglyceride deposit cardiomyovasculopathy (TGCV) is a rare and newly identified disease among patients requiring cardiac transplantation. TGCV is characterized by cardiomyocyte steatosis and triglyceride (TG)-deposit atherosclerosis, resulting from the abnormal intracellular metabolism of TG. TGCV is classified into ...

journal_title：Biochemical and biophysical research communications

authors： Takagi A,Ikeda Y,Kobayashi K,Kobayashi K,Ikeda Y,Kozawa J,Miyauchi H,Li M,Hashimoto C,Hara Y,Yamaguchi S,Suzuki A,Toda T,Nagasaka H,Hirano KI

更新日期：2018-01-01 00:00:00

abstract：:17α-estradiol is a hormonally inactive isomer of 17β-estradiol, but with similar potency as neuroprotector. However, we have previously reported that pretreatment with high concentration (10 μM) of both estrogens abolishes their neuroprotection in rat cerebellar granule neurons. Here, we have examined neuroprotective ...

journal_title：Biochemical and biophysical research communications

authors： Sørvik IB,Paulsen RE

更新日期：2017-08-26 00:00:00

abstract：:Ultraviolet, fluorescence and CD spectral analysis suggested unusual structural features of Rhodotorula gracilis ATCC 90950 DNA. R. gracilis DNA exhibited 13% hyperchromicity at 260 nm as against 26% shown by calf thymus DNA. The biphasic melting curve, one phase between 88-92 degrees C and the other between 92-97 deg...

journal_title：Biochemical and biophysical research communications

authors： Vishnuvardhan D,Divakar S,Joseph R

更新日期：1995-06-26 00:00:00

abstract：:Escherichia coli H+-ATPase (ECF1) was inactivated in a time- and concentration-dependent manner by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), a selective carboxyl group reagent. Among the subunits of ECF1, only the beta subunit was modified by EEDQ. The reaction of 1 mol of EEDQ per mol of ECF1 resulted in...

journal_title：Biochemical and biophysical research communications

authors： Satre M,Dupuis A,Bof M,Vignais PV

更新日期：1983-07-29 00:00:00

abstract：:Matrix metalloproteinase-9 (MMP-9) plays a major role in the pathogenesis of atherosclerosis and restenosis by regulating both migration and proliferation of vascular smooth muscle cells (VSMC) after an arterial injury. In this study, we examined the inhibitory effect of three major flavonoids in Scutellariae Radix, b...

journal_title：Biochemical and biophysical research communications

authors： Lee SO,Jeong YJ,Yu MH,Lee JW,Hwangbo MH,Kim CH,Lee IS

更新日期：2006-12-08 00:00:00

abstract：:Pyrene, a structural analog of benzo[a]pyrene, is an effective competing ligand for high affinity carcinogen binding proteins in mouse liver. A pyrene-derivatized Sepharose gel was prepared for affinity chromatography purification of these proteins, and adsorbs all detectable [3H]B[a]P-binding activity from hepatic cy...

journal_title：Biochemical and biophysical research communications

authors： Collins S,Altman JD,Marletta MA

更新日期：1985-05-31 00:00:00

abstract：:We examined the effect of Mdm2 on regulation of the ApoCIII promoter and its cross-talk with p53 and nuclear receptor SHP. Overexpression of Mdm2 markedly enhanced ApoCIII promoter activity by HNF4α. A direct association of Mdm2 protein with the HNF4α protein was observed by co-immunoprecipitation. Ectopic expression ...

journal_title：Biochemical and biophysical research communications

authors： Yang Z,Zhang Y,Wang L

更新日期：2012-01-13 00:00:00

abstract：:The asymmetric transbilayer distribution of phospholipids in the plasma membrane and the regulation of phosphatidylserine (PS) exposure at the cell surface of animal cells are of high physiological significance. It has been shown previously that annexin V is one of the most sensitive tools with which the presence of s...

journal_title：Biochemical and biophysical research communications

authors： Geldwerth D,Helley D,de Jong K,Sabolovic' D,Sestier C,Roger J,Pons JN,Freyssinet JM,Devaux PF,Kuypers FA

更新日期：1999-04-29 00:00:00

abstract：:In peplomycin-supersensitive Chinese hamster lung cells, the increase in poly(ADP-ribose) synthesizing activity following peplomycin treatment was significantly reduced as compared with the parental lung cells, suggesting that peplomycin-supersensitive lung cells may have some deficiency in DNA repair. On the contrary...

journal_title：Biochemical and biophysical research communications

authors： Ozawa S,Suzuki H,Yamaki H,Nishimura T,Tanaka N

更新日期：1988-03-30 00:00:00

abstract：:Using a subtraction cloning approach we had previously isolated a series of murine cDNA clones representing the genes predominantly expressed in the embryonic brain and down-regulated during development. We now report that one of these cDNA clones encodes a novel type of GTP-binding protein. The predicted protein of 4...

journal_title：Biochemical and biophysical research communications

authors： Sazuka T,Tomooka Y,Ikawa Y,Noda M,Kumar S

更新日期：1992-11-30 00:00:00

abstract：:It has long been assumed that Ca2+ are translocated from the cytosol to the cell nucleus by a long distance to activate transcription machinery buried deep in the nucleoplasm. However, this model has been recently challenged. When HeLa cells were loaded with fluo-3, highly fluorescent spots of approximately 2 microns ...

journal_title：Biochemical and biophysical research communications

authors： Lui PP,Kong SK,Kwok TT,Lee CY

更新日期：1998-06-09 00:00:00

abstract：:The envelope glycoprotein E2 is the major immunodominant protein of the classical swine fever virus and can induce neutralizing antibodies and protective host-immune responses in infected swine. We designed, expressed, and purified multi-epitope protein (GST-BT22) that contains a tandem repeat of the E2 antigenic-dete...

journal_title：Biochemical and biophysical research communications

authors： Tian H,Hou X,Liu X

更新日期：2013-02-08 00:00:00

abstract：:The PCR analysis followed by sequence alignment showed that both the mineralocorticoid receptor (MCR) and the epithelial sodium channel (ENaC) genes were expressed in the human vascular endothelial cell line (ECV). The growth and multiplication of the ECV in culture were influenced by both aldosterone and the MCR-spec...

journal_title：Biochemical and biophysical research communications

authors： Golestaneh N,Klein C,Valamanesh F,Suarez G,Agarwal MK,Mirshahi M

更新日期：2001-02-09 00:00:00

abstract：:Glycine-rich RNA-binding proteins play an important role in post-transcriptional regulation of gene expression, including RNA processing, and are known to be up-regulated in response to a number of external stimuli. However, their regulation in response to salinity stress has not been reported. We have isolated a ligh...

journal_title：Biochemical and biophysical research communications

authors： Aneeta,Sanan-Mishra N,Tuteja N,Kumar Sopory S

更新日期：2002-09-06 00:00:00

abstract：:Microbial redox reactions of inorganic sulfur compounds play a vital role in balancing the turnover of this element in the environment. These vital reactions are carried out by the enzyme system encoded by the sox operon. The central player of the sulfur oxidation biochemistry is the SoxY-Z protein complex. Another pr...

journal_title：Biochemical and biophysical research communications

authors： Bagchi A

更新日期：2011-10-22 00:00:00

abstract：:A recent report has shown that in vitro the RAMP2/CRLR complex is a functional adrenomedullin receptor in human endothelial and vascular smooth muscle cells. However, in vivo, it is well known that CGRP receptors are expressed in human coronary arteries and that a beneficial effect is observed in patients after CGRP i...

journal_title：Biochemical and biophysical research communications

authors： Frayon S,Cueille C,Gnidéhou S,de Vernejoul MC,Garel JM

更新日期：2000-04-21 00:00:00

abstract：:We established two monoclonal antibodies (mAbs) which specifically recognize human 'functionally inactive' recombinant IL-18, and IL-18 protein polymorphism was examined using human monocytes and macrophages (M phi). In 6 day GM-CSF-treated M phi, an 'inactive' IL-18-recognizing mAb 21 detected the IL-18 proform (24 k...

journal_title：Biochemical and biophysical research communications

authors： Kikkawa S,Matsumoto M,Shida K,Fukumori Y,Toyoshima K,Seya T

更新日期：2001-02-23 00:00:00

abstract：:To examine the possible role of lipids as adhesion receptors for infection, Chlamydia trachomatis and Chlamydia pneumoniae were labeled with 125I and layered on thin-layer chromatograms (tlc) of separated lipids isolated from target cells, and bound bacteria were detected by autoradiography. Elementary bodies from bot...

journal_title：Biochemical and biophysical research communications

authors： Krivan HC,Nilsson B,Lingwood CA,Ryu H

更新日期：1991-03-29 00:00:00

abstract：:Treatment of human peripheral blood monocytes with oxidized (oxLDL), minimally modified low-density lipoprotein (mmLDL) or lysophosphatidylcholine (LPC) for 24h was associated with macrophage-like differentiation, as assessed by morphology/CD14 expression. Suppression of these effects by staurosporin (STS) indicated t...

journal_title：Biochemical and biophysical research communications

authors： Weber C,Erl W,Weber PC

更新日期：1995-01-17 00:00:00

abstract：:The gene coding for rat group II phospholipase A2 was isolated from a rat genomic library by using the cDNA for rat platelet phospholipase A2 as a hybridization probe. The rat group II phospholipase A2 gene spanned about 3.5 kilobase pairs and consisted of five exons. Southern blot analysis revealed that a single copy...

journal_title：Biochemical and biophysical research communications

authors： Komada M,Kudo I,Inoue K

更新日期：1990-05-16 00:00:00