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Detection of phosphatidylserine surface exposure on human erythrocytes using annexin V-ferrofluid.

Abstract：

:The asymmetric transbilayer distribution of phospholipids in the plasma membrane and the regulation of phosphatidylserine (PS) exposure at the cell surface of animal cells are of high physiological significance. It has been shown previously that annexin V is one of the most sensitive tools with which the presence of small amounts of PS on the outer surface of eukaryotic cells can be detected. We present here the covalent coupling of annexin V molecules to magnetic nanoparticles of maghemite. The resulting annexin V-ferrofluid is used in the magnetic separation of PS exposing cells, as illustrated for human erythrocytes modified in their phospholipid transbilayer asymmetry by the use of a calcium ionophore. Results on stored human erythrocytes and comparison with results obtained using iodinated and fluorescein-labeled annexin V are also presented.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Geldwerth D,Helley D,de Jong K,Sabolovic' D,Sestier C,Roger J,Pons JN,Freyssinet JM,Devaux PF,Kuypers FA

doi

10.1006/bbrc.1999.0620

subject

Has Abstract

pub_date

1999-04-29 00:00:00

pages

199-203

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(99)90620-4

journal_volume

258

pub_type

杂志文章

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