当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > The ColE2-P9 Rep protein binds to the origin DNA as a monomer.

The ColE2-P9 Rep protein binds to the origin DNA as a monomer.

Abstract：

:The Rep proteins of some plasmid replicons have two functions. Dimers bind to the operator sequences acting as auto-repressors, whereas monomers bind to the iterons to initiate replication of DNA. The ColE2 Rep proteins are present mostly in a dimeric form with some multimers larger than dimers in solution, while the form of Rep binding to Ori is not known. We used an EMSA-based method to determine the molecular weight of Rep in the Rep-Ori complex. The result suggested that Rep binds to Ori as a monomer. In addition, the result of EMSA using the Rep protein fused with the maltose binding protein and the His6-tag also supported this conclusion. We proposed that dimerization of Rep might probably be involved in keeping the copy number of the ColE2 plasmid at the normal low level by limiting the amount of active monomeric forms of Rep in the host cell.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Han M,Aoki K,Yagura M,Itoh T

doi

10.1016/j.bbrc.2006.12.015

subject

Has Abstract

pub_date

2007-02-09 00:00:00

pages

306-10

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(06)02654-4

journal_volume

353

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Participation of cysteine 30 residue in the folding process of ovalbumin evaluated in a refolding experiment using cysteine mutants.

    abstract：:To understand the role of cysteine (SH) residues in the folding of hen ovalbumin (OVA), SH-mutated OVAs, in which each SH residue was replaced by alanine (C11A, C30A, C367A, and C382A), were prepared. SDS-PAGE analysis under non-reducing conditions showed that the C11A and C30A mutants produced a disulfide (SS) isomer...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.11.146

    authors： Ishimaru T,Ito K,Tanaka M,Matsudomi N

    更新日期：2018-01-01 00:00:00

  • Isolation of laminin from human placental basement membranes: amnion, chorion and chorionic microvessels.

    abstract：:Laminin components were solubilized from basement membranes of amnion, chorion and chorionic microvessels of human placenta without prior protease digestion. These structures, after isolation, were initially processed in a sonicator bath containing a solution of Triton X-100, EDTA and 2M NaCl and the laminins extracte...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(83)91730-8

    authors： Ohno M,Martinez-Hernandez A,Ohno N,Kefalides NA

    更新日期：1983-05-16 00:00:00

  • Clathrin-coated vesicles from human placenta contain GTP-binding proteins.

    abstract：:Biochemical and morphological techniques were used to investigate the GTP-binding proteins of clathrin-coated vesicles. Binding of [3H]GTP to clathrin coats was demonstrated by electron microscopic autoradiography. Purified coated vesicles bound 5.2 pmol [35S]GTP gamma S/mg protein. Addition of GTP or GTP gamma S, but...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90505-2

    authors： Lenhard JM,Levy MA,Stahl PD

    更新日期：1991-01-15 00:00:00

  • The miR-599 promotes non-small cell lung cancer cell invasion via SATB2.

    abstract：:MicroRNAs (miRNAs) play important roles in the pathogenesis of many types of cancers by negatively regulating gene expression at posttranscriptional level. Here, we identified that miR-599 is up-regulated in non-small cell lung cancer (NSCLC) patients. It promoted NSCLC cell proliferation by negatively regulating SATB...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,收录出版

    doi：10.1016/j.bbrc.2017.02.005

    authors： Tian W,Wang G,Liu Y,Huang Z,Zhang C,Ning K,Yu C,Shen Y,Wang M,Li Y,Wang Y,Zhang B,Zhao Y

    更新日期：2017-03-25 00:00:00

  • CYP4T1--a cytochrome P450 expressed in rainbow trout (Oncorhynchus mykiss) liver.

    abstract：:Total RNA isolated from a rainbow trout (Oncorhynchus mykiss) liver was subjected to RT/PCR using degenerate primers designed from homologous regions amongst cytochrome P450 CYP4 proteins. PCR amplification resulted in a single electrophoretic band which was excised, purified and sequenced directly, using cycle sequen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6957

    authors： Falckh PH,Wu QK,Ahokas JP

    更新日期：1997-07-18 00:00:00

  • Jagged-1 juxtamembrane region: biochemical characterization and cleavage by ADAM17 (TACE) catalytic domain.

    abstract：:Ectodomain shedding of membrane receptors and ligands carried out by ADAMs (A disintegrin and metalloprotease) plays a major role in several signaling pathways, including Notch. The grounds of substrate recognition, however, are poorly understood. We demonstrate that a recombinant protein corresponding to the juxtamem...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.02.022

    authors： Coglievina M,Guarnaccia C,Zlatev V,Pongor S,Pintar A

    更新日期：2013-03-22 00:00:00

  • High molecular weight Alzheimer's disease amyloid peptide immunoreactivity in human serum and CSF is an immunoglobulin G.

    abstract：:A radioimmunoassay (RIA) was developed to detect the 4200 Dalton amyloid (A4) peptide or it's precursor (A4P) in human serum or cerebrospinal fluid (CSF). A synthetic peptide containing the first 28 amino acids of the 43 amino acid A4 peptide was covalently coupled to bovine thyroglobulin and a polyclonal antiserum in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)91312-x

    authors： Pardridge WM,Vinters HV,Miller BL,Tourtellotte WW,Eisenberg JB,Yang J

    更新日期：1987-05-29 00:00:00

  • Effects of nucleotide on skeletal muscle myosin unfolding in myofibrils by DSC.

    abstract：:The thermal unfolding of myosin in skeletal muscle myofibrils was studied by differential scanning calorimetry (DSC). In the absence of nucleotide two major transitions with Tm of 52 degrees C and 58 degrees C, and a minor transition with Tm of 19 degrees C were detected. The unfolding can be characterized with a tota...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.2816

    authors： Lörinczy D,Belagyi J

    更新日期：1995-12-14 00:00:00

  • Characterization of contractile endothelin and angiotensin receptors in human resistance arteries: evidence for two endothelin and one angiotensin receptor.

    abstract：:Endothelin-1 (ET-1) and angiotensin II (AII) are potent vasoconstrictor hormones which regulate tissue perfusion and blood pressure. We pharmacologically characterized endothelin and angiotensin receptors mediating contractions of human mammary resistance arteries in myographs for isometric tension recording. ET-1 cau...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2514

    authors： Tschudi MR,Lüscher TF

    更新日期：1994-10-28 00:00:00

  • Peptides from pinto bean and red bean with sequence homology to cowpea 10-kDa protein precursor exhibit antifungal, mitogenic, and HIV-1 reverse transcriptase-inhibitory activities.

    abstract：:Peptides with a molecular weight of 5 kDa have been isolated from seeds of the pinto bean and red bean, respectively. The peptides manifest an N-terminal sequence with remarkable resemblance to those of cowpea 10-kDa protein precursor and garden pea disease resistance response protein. The bean peptides possess potent...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.5194

    authors： Ye XY,Ng TB

    更新日期：2001-07-13 00:00:00

  • Characterization of an alveolyn-like protein associated with mouse type 2 pneumocytes using immunochemical methods.

    abstract：:Delipidated proteins from a fraction of mouse lung homogenate enriched for lamellar bodies of type 2 pneumocytes were characterized by a combination of gel filtration and enzyme immunoassay for the presence of a surfactant-associated antigen. Immunoreactivity was associated with a protein of Mr 250-270,000 as well as ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：

    authors： Kumar RK,Smith GJ,Truscott JY,Hristoforidis CP,Lykke AW

    更新日期：1986-09-30 00:00:00

  • Mdm2 is a novel activator of ApoCIII promoter which is antagonized by p53 and SHP inhibition.

    abstract：:We examined the effect of Mdm2 on regulation of the ApoCIII promoter and its cross-talk with p53 and nuclear receptor SHP. Overexpression of Mdm2 markedly enhanced ApoCIII promoter activity by HNF4α. A direct association of Mdm2 protein with the HNF4α protein was observed by co-immunoprecipitation. Ectopic expression ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.12.027

    authors： Yang Z,Zhang Y,Wang L

    更新日期：2012-01-13 00:00:00

  • Prostaglandins antagonize fibroblast proliferation stimulated by tumor necrosis factor.

    abstract：:Tumor necrosis factor (TNF) is known to be a mitogen for human diploid FS-4 fibroblasts. We have shown in an earlier study (Hori et al. (1989) J. Cell. Physiol. 141, 275-280) that indomethacin further enhances the cell proliferation stimulated by TNF. Since indomethacin inhibits the activity of cyclooxygenase, the rol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91482-r

    authors： Hori T,Yamanaka Y,Hayakawa M,Shibamoto S,Tsujimoto M,Oku N,Ito F

    更新日期：1991-01-31 00:00:00

  • Near-infrared fluorescence glucose sensing based on glucose/galactose-binding protein coupled to 651-Blue Oxazine.

    abstract：:Near-infrared (NIR) fluorescent dyes that are environmentally sensitive or solvatochromic are useful tools for protein labelling in in vivo biosensor applications such as glucose monitoring in diabetes since their spectral properties are mostly independent of tissue autofluorescence and light scattering, and they offe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.07.111

    authors： Khan F,Pickup JC

    更新日期：2013-08-30 00:00:00

  • 5-Fluorouracil induces apoptosis through the suppression of NF-kappaB activity in human salivary gland cancer cells.

    abstract：:Activation of the transcription factor NF-kappaB results in protection against apoptosis, and the chemotherapeutic agent 5-Fluorouracil (5-FU) exerts its cytotoxic effect through the induction of apoptosis. Thus, we examined whether 5-FU could induce apoptosis through the suppression of NF-kappaB activity. We found th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3072

    authors： Aota K,Azuma M,Yamashita T,Tamatani T,Motegi K,Ishimaru N,Hayashi Y,Sato M

    更新日期：2000-07-14 00:00:00

  • Protein half-lives of calmodulin and the plasma membrane Ca-ATPase in rat brain.

    abstract：:We report the half-lives for two proteins involved in the regulation of intracellular calcium in the brain: the plasma membrane Ca-ATPase and its regulatory protein, calmodulin. [14C]-labeled leucine was injected into seven month old adult Fischer 344 rats and the time-dependent appearance and loss of radioactivity wa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7105

    authors： Ferrington DA,Chen X,Krainev AG,Michaelis EK,Bigelow DJ

    更新日期：1997-08-08 00:00:00

  • Interstitial chromatin alteration causes persistent p53 activation involved in the radiation-induced senescence-like growth arrest.

    abstract：:Various stresses including ionizing radiation give normal human fibroblasts a phenotype of senescence-like growth arrest (SLGA), manifested by p53-dependent irreversible G1 arrest. To determine the mechanism of persistent activation of p53, we examined phosphorylated Ataxia telangiectasia mutated (ATM) and phosphoryla...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.167

    authors： Suzuki M,Suzuki K,Kodama S,Watanabe M

    更新日期：2006-02-03 00:00:00

  • Murine bone morphogenetic protein-4 gene: existence of multiple promoters and exons for the 5'-untranslated region.

    abstract：:A murine genomic clone of about 16 kilobases encompassing putatively entire bone morphogenetic protein (BMP)-4 gene has been isolated. Comparison with the cDNA clones revealed the existence of at least five exons on the 3' side of the clone. There are two alternative transcriptional start sites, one is for the first e...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1523

    authors： Kurihara T,Kitamura K,Takaoka K,Nakazato H

    更新日期：1993-05-14 00:00:00

  • Differential regulation of phospholipase C-beta isozymes in cardiomyocyte hypertrophy.

    abstract：:Cardiac hypertrophy is a major predictor of heart failure and of morbidity and mortality in developed countries. Many hormones and growth factors induce cardiac hypertrophy via activation of members of the phospholipase C (PLC) family. The expression pattern of the PLCbeta isozyme subfamily was investigated in neonata...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3255

    authors： Schnabel P,Mies F,Nohr T,Geisler M,Böhm M

    更新日期：2000-08-18 00:00:00

  • Decreased cpg15 augments oxidative stress in sleep deprived mouse brain.

    abstract：:Sleep deprivation (SD) has detrimental effects on the physiological function of the brain. However, the underlying mechanism remains elusive. In the present study, we investigated the expression of candidate plasticity-related gene 15 (cpg15), a neurotrophic gene, and its potential role in SD using a REM-SD mouse mode...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.11.132

    authors： Li CJ,Li JJ,Jiang Y,Mu YW,Lu DX,Xiao ZY,Jiang HY,Zhao JJ,Chen XH

    更新日期：2020-02-12 00:00:00

  • Human intestinal VIP receptor: cloning and functional expression of two cDNA encoding proteins with different N-terminal domains.

    abstract：:We describe here two human VIP receptor cDNA clones isolated from a jejunal epithelial cell cDNA library. The hIVR8 cDNA encodes a human VIP receptor consisting of 460 amino acids and has seven putative transmembrane domains like other G protein-coupled receptors. When expressed in COS-7 cells, hIVR8 conferred specifi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1517

    authors： Couvineau A,Rouyer-Fessard C,Darmoul D,Maoret JJ,Carrero I,Ogier-Denis E,Laburthe M

    更新日期：1994-04-29 00:00:00

  • Transplantation of dermal fibroblasts expressing MyoD1 in mouse muscles.

    abstract：:Transplantation of normal myoblasts into dystrophic muscles is a potential treatment for Duchenne muscular dystrophy (DMD). However, the success of such grafts is limited by the immune system responses. To avoid rejection problems, autologous transplantation of the patient's corrected myoblasts has been proposed. Regr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8995

    authors： Huard C,Moisset PA,Dicaire A,Merly F,Tardif F,Asselin I,Tremblay JP

    更新日期：1998-07-30 00:00:00

  • Alpha-ketoglutarate oxidoreductase, an essential salvage enzyme of energy metabolism, in coccoid form of Helicobacter pylori.

    abstract：:In the Krebs cycle of Helicobacter pylori, the absence of alpha-ketoglutarate dehydrogenase and succinyl CoA synthetase are shown. Instead, alpha-ketoglutarate is converted to succinyl CoA and succinate by alpha-ketoglutarate oxidoreductase (KOR) and CoA transferase (CoAT). In the present study, when H. pylori transfo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.08.078

    authors： Tsugawa H,Suzuki H,Nakagawa I,Nishizawa T,Saito Y,Suematsu M,Hibi T

    更新日期：2008-11-07 00:00:00

  • Microvesicles released constitutively from prostate cancer cells differ biochemically and functionally to stimulated microvesicles released through sublytic C5b-9.

    abstract：:We have classified microvesicles into two subtypes: larger MVs released upon stimulation of prostate cancer cells, sMVs, and smaller cMVs, released constitutively. cMVs are released as part of cell metabolism and sMVs, released at 10-fold higher levels, produced upon activation, including sublytic C5b-9. From electron...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.074

    authors： Stratton D,Moore C,Antwi-Baffour S,Lange S,Inal J

    更新日期：2015-05-08 00:00:00

  • Characterization of a novel human gene containing ANK repeats and ARM domains.

    abstract：:We report here on characterization of a novel human gene on chromosome 2q32, containing several ankyrin repeats and ARM domains. The gene FLJ25415, including at least 31 exons and spanning about 90kb of a gene-rich genomic region, is present in the human genome as a single copy. It seems highly conserved along the mam...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.186

    authors： Occhi G,Olivieri M,Rampazzo A,Danieli GA

    更新日期：2004-05-21 00:00:00

  • Study of DFF45 in its role of chaperone and inhibitor: two independent inhibitory domains of DFF40 nuclease activity.

    abstract：:CAD/DFF40, the nuclease responsible for DNA fragmentation during apoptosis, exists as a heterodimeric complex with DFF45/ICAD. This study determines the molecular mechanisms of regulation of DFF40 via the chaperone and inhibition activities of DFF45. We analyze proteins corresponding to the fragments (D1, D2, and D3) ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1497

    authors： McCarty JS,Toh SY,Li P

    更新日期：1999-10-14 00:00:00

  • Leucine-rich repeat-containing G-protein coupled receptor 5 enriched organoids under chemically-defined growth conditions.

    abstract：:An organoid is a complex, multi-cell three-dimensional (3D) structure that contains tissue-specific cells. Epithelial stem cells, which are marked by leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5), have the potential for self-renewal and expansion as organoids. However, in the case of intestinal or...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2018.11.003

    authors： Hahn S,Kim MS,Choi SY,Jeong S,Jee J,Kim HK,Jeong SY,Shin H,Kim HS,Park JS,Yoo J

    更新日期：2019-01-08 00:00:00

  • Small molecule inhibition of arylamine N-acetyltransferase Type I inhibits proliferation and invasiveness of MDA-MB-231 breast cancer cells.

    abstract：:Arylamine N-acetyltransferase 1 is a phase II metabolizing enzyme that has been associated with certain breast cancer subtypes. While it has been linked to breast cancer risk because of its role in the metabolic activation and detoxification of carcinogens, recent studies have suggested it may be important in cell gro...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.01.087

    authors： Tiang JM,Butcher NJ,Minchin RF

    更新日期：2010-02-26 00:00:00

  • EEN regulates the proliferation and survival of multiple myeloma cells by potentiating IGF-1 secretion.

    abstract：:The molecular mechanisms of multiple myeloma are not well defined. EEN is an endocytosis-regulating molecule. Here we report that EEN regulates the proliferation and survival of multiple myeloma cells, by regulating IGF-1 secretion. In the present study, we observed that EEN expression paralleled with cell proliferati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.03.127

    authors： Huang EW,Xue SJ,Li XY,Xu SW,Cheng JD,Zheng JX,Shi H,Lv GL,Li ZG,Li Y,Liu CH,Chen XH,Liu H,Li J,Liu C

    更新日期：2014-05-02 00:00:00

  • SPTLC1 inhibits cell growth via modulating Akt/FOXO1 pathway in renal cell carcinoma cells.

    abstract：:Serine palmitoyltransferase long chain-1 (SPTLC1), which is the rate-limiting enzyme for sphingolipid biosynthesis, has been indicated to be essential for carcinoma cell survival and proliferation in recent, but its role in the regulation of renal cell carcinoma (RCC) remains unknown. In the present study, we found th...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.09.073

    authors： Kong Z,Guo X,Zhao Z,Wu W,Luo L,Zhu Z,Yin S,Cai C,Wu W,Wang D,Liu Y,Duan X

    更新日期：2019-11-26 00:00:00