Leucine-rich repeat-containing G-protein coupled receptor 5 enriched organoids under chemically-defined growth conditions.


:An organoid is a complex, multi-cell three-dimensional (3D) structure that contains tissue-specific cells. Epithelial stem cells, which are marked by leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5), have the potential for self-renewal and expansion as organoids. However, in the case of intestinal organoids from Lgr5-EGFP-IRES-CreERT2 transgenic mice, in vitro expansion of the Lgr5 expression is limited in a culture condition supplemented with essential proteins, such as epidermal growth factor (E), noggin (N), and R-spondin 1 (R). In this study, we hypothesized that self-renewal of Lgr5+ stem cells in a 3D culture system can be stimulated by defined compounds (CHIR99021, Valproic acid, Y-27632, and A83-01). Our results demonstrated that dissociated single cells from organoids were organized into a 3D structure in the four compounds containing the ENR culture medium in a 3D and two-dimensional (2D) culture system. Moreover, the Lgr5 expression level of organoids from the ENR- and compound-containing media increased. Furthermore, the conversion of cultured Lgr5+ stem cells from 2D to 3D was confirmed. Therefore, defined compounds promote the expansion of Lgr5+ stem cells in organoids.


Hahn S,Kim MS,Choi SY,Jeong S,Jee J,Kim HK,Jeong SY,Shin H,Kim HS,Park JS,Yoo J




Has Abstract


2019-01-08 00:00:00














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    abstract::Resonance energy transfer (RET) is widely used to detect proximity between biomolecules. In transparent solution the maximum donor-to-acceptor distance for RET is about 70 A. We measured the effects of metallic silver island films on RET from the intrinsic tryptophan of a protein to a bound probe as the acceptor. Thes...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Malicka J,Gryczynski I,Kusba J,Shen Y,Lakowicz JR

    更新日期:2002-06-21 00:00:00

  • Bovine parathymosin: amino acid sequence and comparison with rat parathymosin.

    abstract::Parathymosin has been purified from calf liver and its primary sequence established, except for a segment containing approximately 11 amino acid residues in the central part of the polypeptide chain. Bovine parathymosin contains approximately 101 amino acid residues and shows 90% identity with rat parathymosin, with s...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Panneerselvam C,Clinton M,Wellner D,Horecker BL

    更新日期:1988-09-15 00:00:00

  • Interleukin-1 receptor antagonist prevents low dose streptozotocin induced diabetes in mice.

    abstract::The effect of the interleukin-1 receptor antagonist (IL-1ra) on development of hyperglycemia and insulitis in mice treated with multiple low doses of streptozotocin (STZ) was evaluated. C57BL/Ks mice were subjected to the following treatments: 1) Injections i.p. of phosphate-buffered saline (PBS) alone; 2) STZ (5 x 40...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sandberg JO,Andersson A,Eizirik DL,Sandler S

    更新日期:1994-07-15 00:00:00

  • Identification of the hRDH-E2 gene, a novel member of the SDR family, and its increased expression in psoriatic lesion.

    abstract::To identify novel psoriasis-associated genes, we focused on several ESTs (expressed sequence tags) whose expression was predominantly increased in the affected skin in patients with psoriasis vulgaris, as assessed by microarray assay. In this paper, a full-length cDNA corresponding to one of those ESTs (AI440266) was ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Matsuzaka Y,Okamoto K,Tsuji H,Mabuchi T,Ozawa A,Tamiya G,Inoko H

    更新日期:2002-10-11 00:00:00

  • OGT functions as a catalytic chaperone under heat stress response: a unique defense role of OGT in hyperthermia.

    abstract::Protein O-GlcNAcylation is proceeded by O-linked GlcNAc transferase (OGT) in nucleocytoplasm and is involved in many biological processes although its physiological role is not clearly defined. To identify the functional significance of O-GlcNAcylation, we investigated heat stress effects on protein O-GlcNAcylation. H...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sohn KC,Lee KY,Park JE,Do SI

    更新日期:2004-09-24 00:00:00

  • Neurotensin regulation of TSH secretion in the rat.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sheppard MC,Askew RD,Shennan KI,Franks S,Ramsden DB

    更新日期:1983-05-31 00:00:00

  • MicroRNA-376c suppresses non-small-cell lung cancer cell growth and invasion by targeting LRH-1-mediated Wnt signaling pathway.

    abstract::MicroRNAs (miRNAs) that negatively regulate gene expression have emerged as novel therapeutic tools for cancer treatment. In this study, we investigated the potential role of Liver receptor homolog-1 (LRH-1), a novel oncogene, in non-small-cell lung cancer (NSCLC), and examined the regulation of LRH-1 by miRNAs. We fo...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Jiang W,Tian Y,Jiang S,Liu S,Zhao X,Tian D

    更新日期:2016-05-13 00:00:00

  • Structural analysis of PseH, the Campylobacter jejuni N-acetyltransferase involved in bacterial O-linked glycosylation.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Song WS,Nam MS,Namgung B,Yoon SI

    更新日期:2015-03-20 00:00:00

  • Porcine guanylin and uroguanylin: cDNA sequences, deduced amino acid sequences, and biological activity of the chemically synthesized peptides.

    abstract::Guanylin and uroguanylin are structurally related intestinal peptide hormones which were purified from a limited number of mammals and are capable of activating the particulate guanylate cyclase-C. Although the biological functions of guanylin and uroguanylin are not yet clarified in detail, they are involved in the r...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Mägert HJ,Hill O,Zucht HD,Martin S,Meyer M,Forssmann WG,Adermann K

    更新日期:1999-05-27 00:00:00

  • Two domains in alpha interferons influence the efficacy of the antiviral response.

    abstract::Murine interferon-alpha 1 and murine interferon-alpha 4 share 80% of their amino acids, yet the proteins differ considerably in their ability to protect mouse or hamster cells against viral infection. With the aim of localizing areas within these proteins which influence the biological response we have constructed hyb...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


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    更新日期:1987-08-31 00:00:00

  • Rapid fragmentation of vimentin in human skin fibroblasts exposed to tamoxifen: a possible involvement of caspase-3.

    abstract::Tamoxifen (TAM), an anti-estrogen compound, is widely used for chemotherapy of breast cancer, although the molecular mechanisms underlying TAM cytotoxicity are obscure. Here, we show that TAM dramatically caused degradation of vimentin (VIM) in human skin fibroblasts, in a time and dose dependent manner. Addition of c...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hashimoto M,Inoue S,Ogawa S,Conrad C,Muramatsu M,Shackelford D,Masliah E

    更新日期:1998-06-18 00:00:00

  • Metabolism and detoxification of TNT by Phanerochaete chrysosporium.

    abstract::Several lines of evidence suggest that TNT detoxification by Phanerochaete chrysosporium is through reduction. Rates of TNT reduction were directly correlated with mycelial mass and TNT concentration. Toxicity was inversely related to the amount of fungus. TNT toxicity was identical in both ligninolytic and nonlignino...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Stahl JD,Aust SD

    更新日期:1993-04-30 00:00:00

  • Cloning and characterization of a novel RNA polymerase II C-terminal domain phosphatase.

    abstract::Reversible phosphorylation of RNA polymerase (RNAP) II's largest subunit C-terminal domain (CTD) is a key event during mRNA metabolism. The CTD phosphatase, FCP1, catalyzes the dephosphorylation of RNAP II and is thought to play a major role in polymerase recycling. In this study, we isolated a novel phosphatase gene ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Zheng H,Ji C,Gu S,Shi B,Wang J,Xie Y,Mao Y

    更新日期:2005-06-17 00:00:00

  • Combination of the chemotherapeutic agent 5-fluorouracil with an inhibitor of its catabolism results in increased micronucleus induction.

    abstract::The rate limiting step in 5-fluorouracil catabolism is catalyzed by the enzyme dihydropyrimidine dehydrogenase. Since degradation of 5-fluorouracil decreases its efficacy in chemotherapy, the inhibition of its catabolism is a promising tool. We investigated the formation of micronuclei in vitro in mouse L5178Y cells. ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Stopper H,Kühnel A,Podschun B

    更新日期:1994-09-15 00:00:00

  • Lead decreases parasitemia and enhances survival of Plasmodium berghei-infected mice.

    abstract::Malaria, a disease accounting for more than one million deaths per year, is caused by intraerythrocytic growth of Plasmodia. Parasitemia may be blunted by suicidal erythrocyte death or eryptosis, which is characterized by cell shrinkage and phosphatidylserine exposure. Triggers of eryptosis include lead nitrate (Pb(NO...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Koka S,Huber SM,Boini KM,Lang C,Föller M,Lang F

    更新日期:2007-11-23 00:00:00

  • CREB-mediated Bcl-2 expression in trichosanthin-induced Hela cell apoptosis.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Wang P,Yan H,Li JC

    更新日期:2007-11-09 00:00:00

  • Anti-influenza virus activity of synthetically polymerized phenylpropenoids.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Sakagami H,Nagata K,Ishihama A,Oh-hara T,Kawazoe Y

    更新日期:1990-11-15 00:00:00

  • Inhibition of protein import into mitochondria by amphiphilic cations: potential targets and mechanism of action.

    abstract::In this paper we describe for the first time the inhibitory effect of three amphiphilic cations, trifluoperazine, propranolol and dibucaine on mitochondrial protein import. The amphiphilic cations did not affect binding of mitochondrial precursor proteins to mitochondria. Import into mitoplasts was affected in a simil...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Pavlov PF,Glaser E

    更新日期:1998-11-09 00:00:00

  • A new approach to possible substrate binding mechanisms for nitrile hydratase.

    abstract::We combined normal mode analysis (NMA) with cavity calculations as a method to get more insight into static crystal structures. We used nitrile hydratase (NHase) as a case study, and the crystal structure of a complex of Pseudonocardia thermophila NHase (1UGP) with n-butyric acid was chosen as a reference structure. T...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Taştan Bishop AO,Sewell T

    更新日期:2006-04-28 00:00:00

  • Sphingomyelin potentiates chemotherapy of human cancer xenografts.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Modrak DE,Lew W,Goldenberg DM,Blumenthal R

    更新日期:2000-02-16 00:00:00

  • Comparative gene expression in the symbiotic and aposymbiotic Aiptasia pulchella by expressed sequence tag analysis.

    abstract::Intracellular symbiotic relationships are prevalent between cnidarians, such as corals and sea anemones, and the photosynthetic dinoflagellate symbionts. However, there is little understanding about how the genes express when the symbiotic relationship is set up. To characterize genes involved in this association, the...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kuo J,Chen MC,Lin CH,Fang LS

    更新日期:2004-05-21 00:00:00

  • Biophysical characterization of Gir2, a highly acidic protein of Saccharomyces cerevisiae with anomalous electrophoretic behavior.

    abstract::Gir2 is an uncharacterized protein of Saccharomyces cerevisiae, containing a RWD/GI domain. In this work, we report the biophysical characterization of Gir2. His-tagged Gir2, expressed and purified from Escherichia coli, showed an abnormally slow migration on SDS-PAGE. The yeast expressed protein behaves similarly. Us...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Alves VS,Pimenta DC,Sattlegger E,Castilho BA

    更新日期:2004-01-30 00:00:00

  • Expression of death receptor 4 induces caspase-independent cell death in MMS-treated yeast.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Kang MS,Lee SK,Park CS,Kang JH,Bae SH,Yu SL

    更新日期:2008-11-14 00:00:00

  • Comparison of polyadenylic acid and oligouridylic acid messenger RNA associated proteins.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Ford G,Nichols JL

    更新日期:1984-05-16 00:00:00

  • Phosphorylation and chromatin mechanics: the central importance of substrate conformation in determining the patterns of HL-60 nuclear phosphorylation.

    abstract::HL-60 nuclear autophosphorylation was studied in vitro under circumstances in which the conformation of chromatin was manipulated with both polyamines and DNAse-I. A general re-arrangement of the phosphorylation patterns occurred as polyamines were removed and nuclei were observed to expand. DNAse-I treatment reduced ...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Testori A,Skinner JD,Murray AW,Burgoyne LA

    更新日期:1991-10-15 00:00:00

  • ERC-55, a binding protein for the papilloma virus E6 oncoprotein, specifically interacts with vitamin D receptor among nuclear receptors.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Imai T,Matsuda K,Shimojima T,Hashimoto T,Masuhiro Y,Kitamoto T,Sugita A,Suzuki K,Matsumoto H,Masushige S,Nogi Y,Muramatsu M,Handa H,Kato S

    更新日期:1997-04-28 00:00:00

  • A role for Rab5 activity in the biogenesis of endosomal and lysosomal compartments.

    abstract::Rab5 is a small GTPase that plays roles in the homotypic fusion of early endosomes and regulation of intracellular vesicle transport. We show here that expression of GFP-tagged GTPase-deficient form of Rab5b (Rab5bQ79L) in NRK cells results in the sequential formation of three morphologically and functionally distinct...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hirota Y,Kuronita T,Fujita H,Tanaka Y

    更新日期:2007-12-07 00:00:00

  • Monitoring of the ethionamide pro-drug activation in mycobacteria by (1)H high resolution magic angle spinning NMR.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Hanoulle X,Wieruszeski JM,Rousselot-Pailley P,Landrieu I,Baulard AR,Lippens G

    更新日期:2005-06-03 00:00:00

  • alpha-Mannosidase from Trichoderma reesei participates in the postsecretory deglycosylation of glycoproteins.

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    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Eneyskaya EV,Kulminskaya AA,Savel'ev AN,Shabalin KA,Golubev AM,Neustroev KN

    更新日期:1998-04-07 00:00:00

  • Growth inhibition of HL-60 cells by extracellular ATP: concentration-dependent involvement of a P2 receptor and adenosine generation.

    abstract::A single addition of ATP (20-1000 microM) to cultures of HL-60 cells resulted here in permanent, Ca(2+)-independent inhibition of cellular proliferation, evident 48 h following treatment. Extracellular ATP (ATPo) was maximally effective at 250 microM giving 90 +/- 1.5% growth inhibition. Up to a concentration of 250 m...

    journal_title:Biochemical and biophysical research communications

    pub_type: 杂志文章


    authors: Seetulsingh-Goorah SP,Stewart BW

    更新日期:1998-09-18 00:00:00