Participation of cathepsins B and D in apoptosis of PC12 cells following serum deprivation.

abstract：:The effects of chlorpromazine on either the activity of mouse brain nitric oxide synthase or the induction of lung nitric oxide synthase in mice and rats were studied. Chlorpromazine inhibited the nitric oxide synthase activity in mouse brain cytosol. This effect could be reversed by adding an excess of calmodulin. In...

journal_title：Biochemical and biophysical research communications

authors： Palacios M,Padron J,Glaria L,Rojas A,Delgado R,Knowles R,Moncada S

更新日期：1993-10-15 00:00:00

abstract：:Cartilage-derived growth factors, enhance proteoglycan synthesis in cultured chick-embryo chondrocytes, and have almost no effect on cell proliferation. Addition of cartilage derived growth factors to cartilage cells loaded with the fluorescent Ca2+ indicator quin 2, caused a rapid, concentration dependent decrease in...

journal_title：Biochemical and biophysical research communications

authors： Eilam Y,Beit-Or A,Nevo Z

更新日期：1985-10-30 00:00:00

abstract：:Recent evidence suggested that human cardiac stem cells (hCSCs) may have the clinical application for cardiac repair; however, their characteristics and the regulatory mechanisms of their growth have not been fully investigated. Here, we show the novel property of hCSCs with respect to their origin and tissue distribu...

journal_title：Biochemical and biophysical research communications

authors： Tateishi K,Ashihara E,Honsho S,Takehara N,Nomura T,Takahashi T,Ueyama T,Yamagishi M,Yaku H,Matsubara H,Oh H

更新日期：2007-01-19 00:00:00

abstract：:Cells of the monocyte series respond to follicle stimulating hormone (FSH) by poorly characterized mechanisms. We studied FSH-receptors (FSH-R) and FSH response in nontransformed human monocytes and in osteoclasts differentiated from these cells. Western blot and PCR confirmed FSH-R expression on monocytes or osteocla...

journal_title：Biochemical and biophysical research communications

authors： Robinson LJ,Tourkova I,Wang Y,Sharrow AC,Landau MS,Yaroslavskiy BB,Sun L,Zaidi M,Blair HC

更新日期：2010-03-26 00:00:00

abstract：:Human dendritic cells (DC) are key regulators of innate and adaptive immunity that can be divided in at least three major subpopulations: plasmacytoid DC (pDC), myeloid type 1 DC (mDC1) and myeloid type 2 DC (mDC2) exhibiting different functions. However, research, diagnostic and cell therapeutic studies on human DC s...

journal_title：Biochemical and biophysical research communications

authors： Schütz F,Hackstein H

更新日期：2014-01-10 00:00:00

abstract：:In order to establish a working cellular model for the study of post-receptor signaling events, insulin-dependent phosphatidylinositol 3' (PtdIns 3')-kinase activity was investigated in circulating mononuclear cells from normal subjects. The p85 alpha regulatory subunit of PtdIns 3'-kinase co-precipitated with activat...

journal_title：Biochemical and biophysical research communications

authors： Renard E,Grigorescu F,Lavabre C,Kahn CR

更新日期：1995-04-06 00:00:00

abstract：:Bacillus thuringiensis var. israelensis produces 130 kDa delta-endotoxin which is highly toxic to mosquito-larvae. The mosquito-larvicidal activity was delineated by sequential deletions from ends of the 1136 amino acids delta-endotoxin. A maximum of 459 amino acids could be removed from the carboxy-terminal of the to...

journal_title：Biochemical and biophysical research communications

authors： Pao-intara M,Angsuthanasombat C,Panyim S

更新日期：1988-05-31 00:00:00

abstract：:The Cdc6 protein is an essential regulator for initiation of DNA replication. Following the G1/S transition, Cdc6 is degraded through a ubiquitin-mediated proteolysis pathway. In this study, we tagged Cdc6 with green fluorescent protein (GFP) and used site-specific mutations to study the regulation of Cdc6 localizatio...

journal_title：Biochemical and biophysical research communications

authors： Luo KQ,Elsasser S,Chang DC,Campbell JL

更新日期：2003-07-11 00:00:00

abstract：:Cellular uptake of succinylated catalase (Suc-CAT; Mw 227 kDa), bovine serum albumin (Suc-BSA; Mw 70 kDa), superoxide dismutase (Suc-SOD; Mw 34 kDa) and soybean trypsin inhibitor (Suc-STI; Mw 21 kDa) was studied using primary cultures of bovine brain microvessel endothelial cells (BMECs) developed as an in vitro model...

journal_title：Biochemical and biophysical research communications

authors： Tokuda H,Masuda S,Takakura Y,Sezaki H,Hashida M

更新日期：1993-10-15 00:00:00

abstract：:The cDNA encoding a swelling-induced chloride conductance regulatory protein, plcln, was cloned from rabbit ciliary epithelium by using a polymerase chain reaction (PCR)-based approach. The open reading frame encoding 236 amino acids possesses high amino acid identity (93/%) with the previously cloned plcln from human...

journal_title：Biochemical and biophysical research communications

authors： Wan XL,Chen S,Sears M

更新日期：1997-10-29 00:00:00

abstract：:Normal human serum transferrin is present in several isoforms, due to differences in glycosylation. Transferrin has two potential glycosylation sites, both normally occupied by oligosaccharide chains. Two of the transferrin isoforms, called carbohydrate deficient transferrin, are specifically increased in patients wit...

journal_title：Biochemical and biophysical research communications

authors： Landberg E,Påhlsson P,Lundblad A,Arnetorp A,Jeppsson JO

更新日期：1995-05-16 00:00:00

abstract：:Specific receptors for murine TNF have been identified in homogenates of rodent brain. These receptors are saturable and bind TNF with sufficient affinity to ensure occupancy by cytokine elaborated during infection. 125I-mTNF was detected in four specific complexes of Mr 130,000, 90,000, 66,000 and 60,000 after affini...

journal_title：Biochemical and biophysical research communications

authors： Kinouchi K,Brown G,Pasternak G,Donner DB

更新日期：1991-12-31 00:00:00

abstract：:Many factors regulate the expression of specialised secondary metabolite biosynthetic gene clusters, which have been recognised as important for the discovery of novel microbial natural products. A cosmid library based on genomic DNA of the marine-derived Streptomyces puniceus Act1085 was constructed and screened to i...

journal_title：Biochemical and biophysical research communications

authors： Park W,Woo JK,Shin J,Oh KB

更新日期：2017-08-26 00:00:00

abstract：:We have characterized a novel surface protein from urea extract of whole cells of group A Streptococcus pyogenes (GAS). A major protein band (35kD) was found to hybridize with human IgG by Western blotting. A search of the N-terminal amino acid sequence of this protein by using the GAS genome sequence database reveale...

journal_title：Biochemical and biophysical research communications

authors： Kawabata S,Tamura Y,Murakami J,Terao Y,Nakagawa I,Hamada S

更新日期：2002-09-06 00:00:00

abstract：:MicroRNAs (miRNAs), small single-stranded regulatory RNAs capable of interfering with intracellular mRNAs that contain partial complementarity, are useful for the design of new therapies against cancer polymorphism and viral mutation. MiRNA was originally discovered in the intergenic regions of the Caenorhabditis eleg...

journal_title：Biochemical and biophysical research communications

authors： Ying SY,Lin SL

更新日期：2005-01-21 00:00:00

abstract：:Human hepatic lipase (HL) is known to bind to the cell surface of hepatocytes and the sinusoidal endothelium of the liver. In each case, it appears that the enzyme remains associated with the cell surface through an ionic interaction with heparan sulfate proteoglycans. However, it remains unclear as to which residues ...

journal_title：Biochemical and biophysical research communications

authors： Yu W,Hill JS

更新日期：2006-05-05 00:00:00

abstract：:Signaling by stem cell factor and Kit, its receptor, play important roles in gametogenesis, hematopoiesis, mast cell development and function, and melanogenesis. Moreover, human and mouse embryonic stem cells express Kit transcripts. Stem cell factor exists as both a soluble and a membrane-bound glycoprotein while Kit...

journal_title：Biochemical and biophysical research communications

authors： Roskoski R Jr

更新日期：2005-12-23 00:00:00

abstract：:After proliferative activation of rat liver cells in vivo by a partial hepatectomy a decrease of the calmodulin content in the three plasma membrane domains (blood sinusoidal, canalicular and lateral) was observed. At 24 hours after partial hepatectomy calmodulin was found to be 3 fold lower in the sinusoidal and late...

journal_title：Biochemical and biophysical research communications

authors： Pujades C,Bastos R,Enrich C,Bachs O

更新日期：1990-12-31 00:00:00

abstract：:Regarding the molecular mechanism of dynamin in receptor-mediated endocytosis, GTPase activity of dynamin has been thought to have a critical role in endocytic vesicle internalization. However, a recent report suggested that GTP-binding to dynamin itself activates the dynamin to recruit molecular machinery necessary f...

journal_title：Biochemical and biophysical research communications

authors： Jeong MJ,Yoo J,Lee SS,Lee KI,Cho A,Kwon BM,Moon MJ,Park YM,Han MY

更新日期：2001-04-27 00:00:00

abstract：:The family C G protein-coupled receptor (GPCR) T1R2 and T1R3 heterodimer functions as a broadly acting sweet taste receptor. Perception of sweet taste is a species-dependent physiological process. It has been widely reported that New World monkeys and rodents are not able to perceive some of the artificial sweeteners ...

journal_title：Biochemical and biophysical research communications

authors： Liu B,Ha M,Meng XY,Khaleduzzaman M,Zhang Z,Li X,Cui M

更新日期：2012-10-19 00:00:00

abstract：:The amino acid sequences in the NH2-terminal region and some other parts of human gastric cathepsin E were investigated. The NH2-terminal sequencing revealed that the cathepsin E preparation which had been activated at pH 4.0 contained one major and one minor isozymes in an approximate molar ratio of 3:1. The NH2-term...

journal_title：Biochemical and biophysical research communications

authors： Athauda SB,Matsuzaki O,Kageyama T,Takahashi K

更新日期：1990-04-30 00:00:00

abstract：:Lysine-specific demethylase 1 (LSD1) is upregulated in many cancers, especially neuroblastoma. We set out to explore whether geranylgeranoic acid (GGA) inhibits LSD1 activity by using recombinant human LSD1. GGA inhibited LSD1 activity with IC50 similar to that of the clinically used drug tranylcypromine. In human neu...

journal_title：Biochemical and biophysical research communications

authors： Sakane C,Okitsu T,Wada A,Sagami H,Shidoji Y

更新日期：2014-01-31 00:00:00

abstract：:We found new photochemical intermediate of third rhodopsin-like pigment (tR) or slow cycling rhodopsin-like pigment (sR) in Halobacterium halobium, which was produced by simultaneous illumination with red and blue light. This illumination is employed for measurements of negative phototaxis. The formation of this inter...

journal_title：Biochemical and biophysical research communications

authors： Tomioka H,Kamo N,Takahashi T,Kobatake Y

更新日期：1984-09-28 00:00:00

abstract：:Alcohol dehydrogenase (ADH; EC 1.1.1.1) and aldehyde dehydrogenase (ALDH; EC 1.2.1.3.) are important enzymes involved in the biotransformation of both alcohols and aldehydes. Today, six classes of ADH and twelve classes of ALDH have been defined in mammals. Here we report the detection and localisation of three classe...

journal_title：Biochemical and biophysical research communications

authors： Cheung C,Smith CK,Hoog JO,Hotchkiss SA

更新日期：1999-07-22 00:00:00

abstract：:Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted ...

journal_title：Biochemical and biophysical research communications

authors： Korc M,Matrisian LM,Planck SR,Magun BE

更新日期：1983-03-29 00:00:00

abstract：:Ceramide, the backbone of sphingolipids, has been reported to be involved in various cellular responses including apoptosis. We recently established and characterized a C2-ceramide-resistant HL-60 subline designated HL-CR. HL-CR cells were resistant to not only ceramide but also anti-cancer drugs including daunorubici...

journal_title：Biochemical and biophysical research communications

authors： Sawai H,Kawai S,Domae N

更新日期：2004-06-18 00:00:00

abstract：:In the early postnatal period, cerebellar granule cells exhibit an activity-dependent downregulation of a set of immaturation genes involved in cell growth and migration and are shifted to establishment of a mature network formation. Through the use of a granule cell culture and both pharmacological and RNA interferen...

journal_title：Biochemical and biophysical research communications

authors： Okazawa M,Abe H,Nakanishi S

更新日期：2016-05-13 00:00:00

abstract：:Several lines of evidence provided by other workers indicate that within the same species thyroid hormone binding protein, the beta-subunit of prolyl hydroxylase, and protein disulfide isomerase are the same protein. We sought to determine if glycosylation site binding protein, a lumenal protein of the endoplasmic ret...

journal_title：Biochemical and biophysical research communications

authors： Kimura H,Noiva R,Mizunaga T,Yamauchi K,Horiuchi R,Cheng SY,Lennarz WJ

更新日期：1990-08-16 00:00:00

abstract：:The nucleotide sequence for mouse calcitonin was determined from a cDNA obtained using a polymerase chain reaction (PCR) based method, the rapid amplification of cDNA ends (RACE)-PCR. Primers designed from highly conserved regions in the coding sequences of known rat and human calcitonin cDNAs were used to amplify cal...

journal_title：Biochemical and biophysical research communications

authors： Rehli M,Luger K,Beier W,Falk W

更新日期：1996-09-13 00:00:00

abstract：:The E. coli ogt O6-alkylguanine-DNA alkyltransferase has two cysteine residues positioned identically with respect to cysteines in the E. coli ada O6-alkylguanine-DNA alkyltransferase. In order to assess their function, these residues were each substituted by a glycine to generate altered forms of the ogt protein. Mut...

journal_title：Biochemical and biophysical research communications

authors： Harris LC,Potter PM,Margison GP

更新日期：1992-08-31 00:00:00