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MicroRNA-2400 promotes bovine preadipocyte proliferation.

Abstract：

:MicroRNAs (miRNAs) play critical roles in the proliferation of bovine preadipocytes. miR-2400 is a novel and unique miRNA from bovines. In the present study, we separated and identified preadipocytes from bovine samples. miR-2400 overexpression increased the rate of preadipocyte proliferation, which was analyzed with a combination of EdU and flow cytometry. Simultaneously, functional genes related to proliferation (PCNA, CCND2, CCNB1) were also increased, which was detected by real-time PCR. Furthermore, luciferase reporter assays showed that miR-2400 bound directly to the 3'untranslated regions (3'UTRs) of PRDM11 mRNA. These data suggested that miR-2400 could promote preadipocyte proliferation by targeting PRDM11.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Wei Y,Cui YF,Tong HL,Zhang WW,Yan YQ

doi

10.1016/j.bbrc.2016.08.038

subject

Has Abstract

pub_date

2016-09-23 00:00:00

pages

1054-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)31293-1

journal_volume

478

pub_type

杂志文章

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