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Enhanced nerve growth factor efficiency in neural cell culture by immobilization on the culture substrate.

Abstract：

:Nerve growth factor (NGF) immobilization on a culture substrate may dramatically reduce the amount of NGF required for pheochromocytoma (PC12) cell culture. Coverslips on which NGF had been immobilized, or with NGF added to the culture medium daily, were used to culture PC12 cells. We examined the effects of adding 5, 10, or 100 ng of NGF to cultures daily, and compared them to the effects of immobilizing 5, 10, or 100 ng of NGF on culture substrates in a single dose. Cultures with 10 or 5 ng NGF added daily showed dramatically decreased cell viability, mitochondrial metabolic activity, and neuronal differentiation compared to cultures with 100 ng NGF added daily, while also exhibiting increased apoptosis. In contrast, a single dose of 100 ng immobilized NGF yielded results similar to 100 ng NGF added daily (total: 300 ng over 3 days), and 10 or 5 ng immobilized NGF showed far better results than 10 or 5 ng NGF added daily. These results demonstrate that NGF immobilization can dramatically reduce the amount of NGF required in neuronal cell culture.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Bhang SH,Lee TJ,Yang HS,La WG,Han AM,Kwon YH,Kim BS

doi

10.1016/j.bbrc.2009.03.016

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

315-20

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(09)00468-9

journal_volume

382

pub_type

杂志文章

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