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Systematic time-dependent visualization and quantitation of the neurogenic rate in brain organoids.

Abstract：

:Organoids mimicking the formation of the brain cortex have been demonstrated to be powerful tools for developmental studies as well as pathological investigations of brain malformations. Here, we report an integrated approach for the quantification of temporal neural production (neurogenic rate) in organoids derived from embryonic brains. Spherical tissue fragments with polarized cytoarchitectures were incubated in multiple cavities arranged in a polymethylmethacrylate chip. The time-dependent neurogenic rate in the organoids was monitored by the level of EGFP under the promoter of Tbr2, a transcription factor that is transiently expressed in neural fate-committed progenitors during corticogenesis. Importantly, our monitoring system exhibited a quick response to DAPT, a drug that promotes neural differentiation. Furthermore, we successfully quantified the temporal neurogenic rate in a large number of organoids by applying image processing that semi-automatically recognized the positions of organoids and measured their signal intensities from sequential images. Taken together, we provide a strategy to quantitate the neurogenic rate in brain organoids in a time-dependent manner, which will also be a potent method for monitoring organoid formation and drug activity in other tissue types.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Kosodo Y,Suetsugu T,Kobayashi TJ,Matsuzaki F

doi

10.1016/j.bbrc.2016.12.187

subject

Has Abstract

pub_date

2017-01-29 00:00:00

pages

94-100

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(16)32261-6

journal_volume

483

pub_type

杂志文章

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