Abstract：
:How rod-shaped bacteria form and maintain their shape is an important question in bacterial cell biology. Results from fluorescent light microscopy have led many to believe that the actin homolog MreB and a number of other proteins form long helical filaments along the inner membrane of the cell. Here we show using electron cryotomography of six different rod-shaped bacterial species, at macromolecular resolution, that no long (> 80 nm) helical filaments exist near or along either surface of the inner membrane. We also use correlated cryo-fluorescent light microscopy (cryo-fLM) and electron cryo-tomography (ECT) to identify cytoplasmic bundles of MreB, showing that MreB filaments are detectable by ECT. In light of these results, the structure and function of MreB must be reconsidered: instead of acting as a large, rigid scaffold that localizes cell-wall synthetic machinery, moving MreB complexes may apply tension to growing peptidoglycan strands to ensure their orderly, linear insertion.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Swulius MT,Chen S,Jane Ding H,Li Z,Briegel A,Pilhofer M,Tocheva EI,Lybarger SR,Johnson TL,Sandkvist M,Jensen GJdoi
10.1016/j.bbrc.2011.03.062subject
Has Abstractpub_date
2011-04-22 00:00:00pages
650-5issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(11)00446-3journal_volume
407pub_type
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