Implanting mouse embryo stain with a LNF-I bearing fluorescent probe at their mural trophectodermal side.

Abstract:

:Mouse embryos at implantation stage were stained successfully with lacto-N-fucopentaose I (LNF-I) bearing neoglycoprotein labeled with rhodamine synthesized by us for the first time. The fluorescent neoglycoproteins carrying LNF-II, -III, LND-I, or LNT failed to stain the embryos. The embryo was stained only at the cell surface of trophectoderm at the mural side. Since the attachment of the mouse embryo to the uteric epithelium occurs at its mural side trophectoderm and LNF-I is the key substance in mouse implantation (Lindenberg, S. et al, (1988) J. Reprod. Fert. 83, 149-158), the material stained with the probe carrying LNF-I appears to be the molecule responsive to attachment to the endometrium surface and leading to implantation.

authors

Yamagata T,Yamazaki K

doi

10.1016/0006-291x(91)92036-j

subject

Has Abstract

pub_date

1991-12-31 00:00:00

pages

1004-9

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)92036-J

journal_volume

181

pub_type

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